• BMB Reports
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• v.48 no.1
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• pp.13-18
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• 2015

Alzheimer's disease severely compromises cognitive function. One of the mechanisms to explain the pathology of Alzheimer's disease has been the hypotheses of amyloid-pore/channel formation by complex $A{\beta}$-aggregates. Clinical studies suggested the moderate alcohol consumption can reduces probability developing neurodegenerative pathologies. A recent report explored the ability of ethanol to disrupt the generation of complex $A{\beta}$ in vitro and reduce the toxicity in two cell lines. Molecular dynamics simulations were applied to understand how ethanol blocks the aggregation of amyloid. On the other hand, the in silico modeling showed ethanol effect over the dynamics assembling for complex $A{\beta}$-aggregates mediated by break the hydrosaline bridges between Asp 23 and Lys 28, was are key element for amyloid dimerization. The amyloid pore/ channel hypothesis has been explored only in neuronal models, however recently experiments suggested the frog oocytes such an excellent model to explore the mechanism of the amyloid pore/channel hypothesis. So, the used of frog oocytes to explored the mechanism of amyloid aggregates is new, mainly for amyloid/pore hypothesis. Therefore, this experimental model is a powerful tool to explore the mechanism implicates in the Alzheimer's disease pathology and also suggests a model to prevent the Alzheimer's disease pathology.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.20 no.1
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• pp.36-44
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• 2010

The accurate prediction of dynamic characteristics of high speed rotors, such as gas turbines, is important to avoid the possibility of operating the machinery near the critical speeds or unstable speed regions. However, the dynamic analysis methods and softwares for gas turbines have been developed in the process of producing many gas turbines by manufacturers and most of them have seldom been disclosed to the public. Recently, commercial FEM softwares, such as SAMCEF, ANSYS and NASTRAN, started supporting some rotordynamics analysis modules based on 3-D finite elements. In this paper, the dynamic analysis method using commercial S/W, especially ANSYS, is attempted for the small-size gas turbine engine rotor, and the analysis capability and limitations of its rotordyamics module are evaluated for further improvement of the module. As the preliminary procedure, the rotordyamic analysis capability of ANSYS was tested and evaluated with the reference models of the well-known dynamics. The limitations in application of the rotordynamics module were then identified. Under the current capability and limitations of ANSYS, it is shown that Lee diagram, a new frequency-speed diagram enhanced with the concept of $H{\infty}$ in rotating machinery, can be indirectly obtained from FRFs computed from harmonic response analysis of ANSYS. Finally, it is demonstrated based on the modeling and analysis method developed in the process of the S/W verification that the conventional Campbell diagram, Lee diagram, mode shapes and critical speeds of the small-size gas turbine engine rotor can be computed using the ANSYS rotordynamics module.

• Journal of the Korean Institute of Intelligent Systems
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• v.25 no.1
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• pp.78-84
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• 2015

Depth estimation is often required for robot vision, 3D modeling, and motion control. Previous method is based on the focus measures which are calculated for a series of image by a single camera at different distance between and object. This method, however, has disadvantage of taking a long time for calculating the focus measure since the mask operation is performed for every pixel in the image. In this paper, we estimates the depth by using the focus measure of the boundary pixels located between the objects in order to minimize the depth estimate time. To detect the boundary of an object consisting of a straight line and a circle, we use the Hough transform and estimate the depth by using the focus measure. We performed various experiments for PCB images and obtained more effective depth estimation results than previous ones.

• Proceedings of the Korea Water Resources Association Conference
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• 2009.05a
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• pp.2220-2224
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• 2009

하천의 수질모의를 위해서는 정상상태 모델인 QUAL2E 가 널리 사용되어 왔다. 그러나 우리나라는 기후 특성상 하절기에 강우가 집중되고 경우에 따라서는 댐등에 의해 장기간 수류의 지체현상이 발생하므로 지역 및 시기에 따라 발생하는 수질 문제가 서로 다를 수 있다. 따라서 우리나라의 대부분의 수체에서는 시간에 따라 모의를 할 수 있는 비정상 상태의 수질모델이 적용되어야 할 필요가 종종 발생한다. 미국공병단에서 개발된 CE-QUAL-W2 모델은 2차원 모델로서 수리동역학과 수질반응역학을 한데 묶어서 풀이하였다는 점에서 획기적인 변화로 볼 수 있다. 이 모델은 종방향으로 길고 수심이 깊으며 상대적으로 하폭이 좁은 형태의 수체에 적합하며 하폭방향의 수질 변화를 나타내는 데 사용되지 못하는 단점이 있다. WASP(Water Quality Analysis and Simulation Program) 은 미국 환경부에서 개발한 비정상상태 3차원 수질모델로서 세계적으로 다양한 수체에 널리 사용되어 왔다. 이 모델에서 1차원적 흐름을 예측할 수 있는 DYNHYD 라는 수리학적 부프로그램은 2차원 또는 3차원 상황에서는 사용할 수가 없었음에 따라 수리학적 고려가 빈약한 것이 이 모델의 가장 큰 약점으로 지적되어 왔다. 최근 미국 환경부는 EFDC(Environmental Fluid Dynamics Code) 라는 3차원 수리동역학 프로그램을 이용하여 대상 수체의 수리학적 거동을 모의하고 그 결과를 WASP7 에 연계시킬 수 있도록 하여 기존의 단점을 대폭 보완하였다. 본 연구에서는 금강 상류에 위치하고 있는 용담호를 대상으로 EFDC 를 이용하여 2005년 1년간 수위 및 수온성층현상을 예측하고 그 결과가 WASP 에 연결되어 사용될 수 있도록 하였다. 적절한 격자의 수를 결정하기 위하여 다양한 경우가 시행착오적으로 시험되었으며 비교적 적은 숫자의 격자로도 수위 및 수온의 모의가 가능하다는 것을 발견하였다.

• Coupled systems mechanics
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• v.1 no.4
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• pp.381-395
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• 2012

Dynamic response of Pile Supported Structures is highly depended on Soil Pile Structure Interaction. In this paper, by comparison of experimental and numerical dynamic responses of a prototype jacket offshore platform for both hinge based and pile supported boundary conditions, effect of soil-pile-structure interaction on dynamic characteristics of this platform is studied. Jacket and deck of a prototype platform is installed on a hinge-based case first and then platform is installed on eight skirt piles embedded on continuum monolayer sand. Dynamic characteristics of platform in term of natural frequencies, mode shapes and modal damping are compared for both cases. Effects of adding and removing vertical bracing members in top bay of jacket on dynamic characteristics of platform for both boundary conditions are also studied. Numerical simulation of responses for the studied platform is also performed for both mentioned cases using capability of ABAQUS and SACS software. The 3D model using ABAQUS software is created using solid elements for soil and beam elements for jacket, deck and pile members. Mohr-Coulomb failure criterion and pile-soil interface element are used for considering nonlinear pile soil structure interaction. Simplified modeling of soil-pile-structure interaction effect is also studied using SACS software. It is observed that dynamic characteristics of the system changes significantly due to soil-pile-structure interaction. Meanwhile, both of complex and simplified (ABAQUS and SACS, respectively) models can predict this effect accurately for such platforms subjected to dynamic loading in small range of deformation.

• Journal of Microbiology and Biotechnology
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• v.28 no.9
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• pp.1482-1492
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• 2018

Fusarium oxysporum trypsin (FOT) is a fungal serine protease similar to mammal trypsin. The FOT could be successfully expressed in Pichiapastoris by engineering the natural propeptide APQEIPN. In this study, we constructed two recombinant enzymes with engineered amino acid sequences added to the N-terminus of FOT and expressed in P. pastoris. The N-terminal residues had various effects on the structural and functional properties of trypsin. The FOT, and the recombinants TE (with peptide YVEF) and TS (with peptide YV) displayed the same optimum temperature ($40^{\circ}C$) and pH (8.0). However, the combinants TE and TS showed significantly increased thermal stability at $40^{\circ}C$ and $50^{\circ}C$. Moreover, the combinants TE and TS also showed enhanced tolerance of alkaline pH conditions. Compared with those of wild-type FOT, the intramolecular hydrogen bonds and the cation ${\pi}$-interactions of the recombinants TE and TS were significantly increased. The recombinants TE and TS also had significantly increased catalytic efficiencies (referring to the specificity constant, $k_{cat}/K_m$), 1.75-fold and 1.23-fold than wild-type FOT. In silico modeling analysis uncovered that the introduction of the peptides YVEF and YV resulted in shorter distances between the substrate binding pocket (D174, G198, and G208) and catalytic triad (His42, Asp102, and Ser180), which would improve the electron transfer rate and catalytic efficiency. In addition, N-terminal residues modification described here may be a useful approach for improving the catalytic efficiencies and characteristics of other target enzymes.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.16 no.3
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• pp.291-299
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• 2018

Under a pyro-processing concept, an electrolytic reduction process has been developed to reduce uranium oxide in molten salt by electrochemical means as a part of spent fuel treatment process development. Accordingly, a model based on electrochemical theory is required to design a reactor for the electrolytic reduction process. In this study, a 1D model based on the phase-field theory, which explains phase separation behaviors was developed to simulate electrolytic reduction of uranium oxide. By adopting parameters for diffusion of oxygen elements in a pellet and electrochemical reaction rate at the surface of the pellet, the model described the behavior of inward reduction well and revealed that the current depends on the internal diffusion of the oxygen element. The model for the electrolytic reduction is expected to be used to determine the optimum conditions for large scale reactor design. It is also expected that the model will be applied to simulate the integration of pyro-processing.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.27 no.5
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• pp.379-384
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• 2014

This article describes the dynamic behaviors of a rubber tired gantry crane(RTGC) under typical load conditions which are used in the design of gantry cranes. In order to investigate the dynamic characteristics of an RTGC, we developed a finite element crane model for its huge structure. The finite element model was validated with the modal test results, e.g., natural frequencies and normal modes. And other components of RTGC were converted into detailed 3D CAD models and finally transformed to rigid body models in a dynamic simulation program ADAMS. The load conditions considered in this paper were a normal operating condition(OP1) and container hanging condition with no external loads. As a result, we could find there was large influence of crane's vibration owing to its structural stiffness and deformation. And the vibration of crane could made the movements of RTGC, which occurs crash or malfunction of crane works.

• Journal of Hydrogen and New Energy
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• v.29 no.1
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• pp.41-55
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• 2018

General polymer electrolyte fuel cell (PEMFC) operates at less than $80^{\circ}C$. Therefore liquid phase water resulting from electrochemical reaction accumulates and floods the cell which in turn increases the mass transfer loss. To prevent the flooding, it is common to employ serpentine flow channel, which can efficiently export liquid phase water to the outlet. The major drawback of utilizing serpentine flow channel is the large pressure drop that happens between the inlet and outlet. On the other hand, in the high temperature polymer electrolyte fuel cell (HT-PEMFC), since the operating temperature is 130 to $180^{\circ}C$, the generated water is in the state of gas, so the flooding phenomenon is not taken into consideration. In HT-PEMFCs parallel flow channel with lower pressure drop between the inlet and outlet is employed therefore, in order to circulate hydrogen and air in the cell less pumping power is required. In this study we analyzed HT-PEMFC's different flow channels by parallel computation using previously developed 3-D isothermal model. All the flow channels had an active area of $25cm^2$. Also, we numerically compared the performance of HT-PEMFC parallel flow channel with different manifold area and Rib interval against the original serpentine flow channel. Results of the analysis are shown in the form of three-dimensional contour polarization curves, flow characteristics in the channel, current density distribution in the Membrane, overpotential distribution in the catalyst layer, and hydrogen and oxygen concentration distribution. As a result, the performance of a real area fuel cell was predicted.

• ALGAE
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• v.30 no.2
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• pp.163-170
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• 2015

Tyrosinase inhibitors are an important component of cosmetic products. Our previous studies have proposed that eckol isolated from the brown alga Ecklonia cava, can be explored as a tyrosinase inhibitor. However, cellular activities and mechanism of action of eckol remain unknown. Therefore, the current study analyzed the eckol binding modes using the crystal structure of Bacillus megaterium tyrosinase. The effects of eckol on melanin synthesis induced by ${\alpha}$-melanocyte stimulating hormone in B16F10 melanoma cells were also investigated. We predicted the 3D structure of tyrosinase and used a docking algorithm to simulate binding between tyrosinase and eckol. These molecular modeling studies were successful (calculated binding energy value, $-115.84kcal\;mol^{-1}$) and indicated that eckol interacts with Asn205, His208, and Arg209. Furthermore, eckol markedly inhibited tyrosinase activity and melanin synthesis in B16F10 melanoma cells. We also found that eckol decreased the expression of tyrosinase, tyrosinase-related protein (TRP) 1, and TRP2. These results indicate that eckol is a potent inhibitor of melanogenesis, and this finding may be useful for the development of novel pharmaceutical and cosmetic agents.