• Biomedical Science Letters
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• v.22 no.1
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• pp.24-28
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• 2016

Tuberculosis (TB) remains an unsolved community health problem since identification of its causing microorganism called Mycobacterium tuberculosis (MTB) by Robert Koch in 1882. Annually, eight million TB cases are newly reported and 2~3 million patients die from TB. Pulmonary TB is highly infectious and untreated pulmonary TB patients are believed to infect >10 people in a year. The conventional methods for diagnosis of TB are chest X-ray and isolation of the causing microorganisms from patient specimens. Screening of TB is conducted with smeared sputum in slides, and TB is confirmed by identification of MTB in cultured specimens. One of the fatal pitfalls of screening detection for smeared sputum is that it is impossible to distinguish MTB and other acid-fast bacilli (AFB) because they are stained equally with Ziehl-Neelsen (ZN) stain. Culture of MTB is the most reliable method for diagnosis of TB but it takes 4~8 weeks. In this report, we suggest a fast and highly-reliable MTB detection method that distinguishes AFB in sputum samples. Purified DNA from the AFB stained slide samples offered by The Korean Institute of Tuberculosis were used to detect infected MTB in patients. PCR, real-time PCR and reverse blot hybridization assay (REBA) methods were applied to purified DNA. Conclusively, the real-time PCR method was confirmed to produce high sensitivity and we were able to further detect drug-resistant MTB with REBA.

• The Journal of the Korea institute of electronic communication sciences
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• v.8 no.12
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• pp.1875-1882
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• 2013

Micro-array technology contributes numerous achievements such as ordering of gene network and integration of genomic. This technology is well established as means for investigating patterns of gene expression. DNA micro-arrays utilize Affymetric chips where a large quantity of DNA sequences may be synthesized. There are two general type of conventional DNA array spotter: contact and piezoelectric. The contact technology used spotting pin technology to make contact with the glass slide surface. This may caused damage or scratches to the surface matrix where protein will be contaminated and may not bind specifically. Piezoelectric technology available at this present time on the other hand requires the analyzer to print the result that can only be done within the laboratory despite of mass production. Therefore, in this paper, high-throughput technology is developed for providing greater consistency in feature spot without touching the glass slide surface.

• Journal of the Korean Society of Costume
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• v.64 no.4
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• pp.162-175
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• 2014

This study focuses on Korea's traditional-style soryebok, in terms of its foundation, progression, and structural features. The research methods employed here include literature analyses of relevant laws and an official daily gazette, and practical analyses of artifacts and photographic documents. Especially, the artifacts of heukdannyeong(black uniform for officials with a round neck) in the form of chaksu(tight sleeves) were examined, which was regulated as soryebok in Eulmiuijegaehyeok. The term "soryebok" firstly appeared in "使和記略"(Sahwagiryak) written by Park Yeong-hyo, a foreign envoy to Japan, in 1882. Emperor's invitation written in the book asked him to wear daeryebok at the ritual and soryebok to the banquet. Soryebok was not adopted in Gapshinuijegaehyeok in 1884. However, several illustrated documents of the modern banquets at that time reveal that sabok, which was included in the 1884 reform, was used as soryebok. According to the Gapouijegaehyeok in December 1894, courtiers were required to wear heukdannyeong as daeryebok, and add juui(topcoat) and dapho(sleeveless coat) as tongsangyebok when they visited the royal palace. In Joseon's first daeryebok system, the term "tongsangyebok" that had been used in Japan was employed before soryebok was used. According to Eulmiuijegaehyeok in August 1895, the term "soryebok" originated from a costume ritual for courtiers of the Joseon Dynasty. Soryebok featured heokbannyeong chaksupo, samo (winged hat for officials), sokdae(belt), and hwaja (shoes for officials). There are around 24 artifact pieces of heukdannyeong in the form of chaksu(tight sleeves) in the following locations: garments of Prince Heungwangun and Wansungun, the court artifacts, Korea University Museum, Yun Ung-ryeol's family housed at Yonsei University, and Kyungwoon Museum. Artifacts have mu(godet) pulled back and a topcoat-like triangular mu. In conclusion, heukdannyeong, traditional-style soryebok has significance in the history of modern dress because streamlined traditional clothes and newly introduced Western dress system were able to be combined.

• KIEE International Transactions on Electrophysics and Applications
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• v.12C no.4
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• pp.201-207
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• 2002

A Pin-Cylinder discharge type ozonizer was designed and manufactured. The increase or decrease of temperature greatly influences on the characteristics of ozone generation of a discharge type ozonizer. The characteristics of ozone concentration ( $O_{3con}$), ozone generation ($_{3g}$) and ozone yield .ate ($_{3Y}$) of the ozonizer were investigated by varying the gas flow rate (Q), the discharge power ($_{Wd}$ ) and the temperature (T). At T = 20($^{\circ}C$), the values of ( $O_{3con}$) were found as 7800,5300,3000 and 2300(ppm) at Q=1,2,4 and 6(1/min) respectively. The corresponding values of ( $O_{3g}$) were found as 917, l247,1411 and 1623(mg/h) and those of ( $O_{3Y}$) were 93,126,143 and 164(g/kWh) respectively. When the temperature is decreased to -50($^{\circ}C$), the values of ( $O_{3con}$) became 12000,8000,5200 and 3600(ppm) at Q=1,2,4 and 6(1/min) respectively. The corresponding values of 0,9 were obtained as 1411,1882,2446 and 2600(mg/h) and those of ($O_{3Y}$) were 143,190,247 and 2631g/kWh] respectively. Hence as the temperature was decreased from 20 to -50[。C], the efficiencies of ozone generation were increased by 54,51,73 and 60[%] at Q=1,2,4 and 6(1/min) respectively.ctively.

• Journal of Microbiology and Biotechnology
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• v.29 no.12
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• pp.1882-1893
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• 2019

β-Glucosidases and β-xylosidases are two categories of enzymes that could cleave out non-reducing, terminal β-D-glucosyl and β-D-xylosyl residues with release of D-glucose and D-xylose, respectively. In this paper, two functional β-glucosidase Dth3 and β-xylosidase Xln-DT from Dictyoglomus thermophilum were heterologously expressed in E.coli BL21 (DE3). Dth3 and Xln-DT were relatively stable at 75℃ and were tolerant or even stimulated by glucose and xylose. Dth3 was highly tolerant to glucose with a K_i value of approximately 3 M. Meanwhile, it was not affected by xylose in high concentration. The activity of Xln-DT was stimulated 2.13-fold by 1 M glucose and 1.29-fold by 0.3 M xylose, respectively. Furthermore, the βglucosidase Dth3 and β-xylosidase Xln-DT showed excellent selectivity to cleave the outer C-6 and C-3 sugar moieties of ASI, which established an effective and green method to produce the more pharmacologically active CAG, an exclusive telomerase activator. We measured temperature, pH and dosage of enzyme using a single-factor experiment in ASI biotransformation. After optimization, the optimal reaction conditions were as follows: 75℃, pH 5.5, 1 U of Dth3 and 0.2 U of Xln-DT, respectively. Under the optimized conditions, 1 g/l ASI was transformed into 0.63 g/l CAG with a corresponding molar conversion of 94.5% within 3 h. This is the first report to use the purified thermostable and sugar-tolerant enzymes from Dictyoglomus thermophilum to hydrolyze ASI synergistically, which provides a specific, environment-friendly and cost-effective way to produce CAG.

• Korean Journal of Plant Taxonomy
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• v.36 no.3
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• pp.227-255
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• 2006

Takenoshin Nakai (1882-1952), Japanese taxonomist and professor of the University of Tokyo, who was one of Korean flora specialists, conducted his plant exploration from 1909 to 1942 after he completed two volumes of Flora Koreana. His collections, which are currently deposited at the University of Tokyo herbarium (TI), are important research resources. Since all collection sites were simply described in his collection sites in Romanized characters based on Japanese pronunciation, it is difficult for Korean as well as Japanese botanists to pinpoint those sites using the current or the old Korean maps. No single literature has been available regarding his collection routes and sites, although this information is essential to current plant systematic research in Korea. From this current study, all locality names were reviewed based on his own collections as well as his written literatures, and those were listed as the order of his collection dates with seven maps here.

• Transactions of the Korean hydrogen and new energy society
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• v.26 no.3
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• pp.212-220
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• 2015

The engineering plastic of sulfonated polyether ether ketone (SPEEK) as a polymer matrix has been developed in this lab to replace Nafion, solid polymer electrolytes of perfluorosulfonic acid membrane which has several flaws such as high cost, and limited operational temperature above $80^{\circ}C$. The SPEEK was prepared in the sulfonation reaction of polyether ether ketone (PEEK). The organic-inorganic blended composite membranes were prepared by sol-gel casting method with loading the highly dispersed ceria and cesium-substituted tungstosilicic acid (Cs-TSiA) with cross-linking agent contents of 0.01 mL. In conclusion, CL-SPEEK/Cs-TSiA/ceria 1% membrane showed the optimum results such as 0.1882 S/cm of proton conductivity at $80^{\circ}C$, and 99.61 MPa of tensile strength which were better than Nafion 117 membrane.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.12A
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• pp.1876-1882
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• 1999

In this paper, we have proposed a FCB detection algorithm for the GSM system which is european cellular standard. The detection algorithm can be implemented using received signal sampler, correlator, and post detection combiner. GSM mobile phone can use the proposed algorithm for detection of the Broadcasting Channel, and to obtain the initial timing estimate. The proposed algorithm has a architecture which is suitable for DSP or ASIC implementation, and required memory size is small. The performance of the algorithm is a function of the processing data window size and the threshold values. Proper window size and the threshold values can be determined by analyzing the correlator and combiner. The proposed algorithm has been implemented using DSP, and the performance was verified using baseband simulation. The simulation assumed frequency offset values of 0ppm and 15ppm with the receiver filter bandwidth set at both minimum and maximum. It is shown that the algorithm is robust under various assumptions, and suitable for real implementations.

• Journal of the Korea Fashion and Costume Design Association
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• v.11 no.1
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• pp.1-16
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• 2009

Ip-yeong or gat-keun decorate heuk-rip, supplementing its simplicity. When the wind blew, a dynamic beauty was created, especially if the gat-keun was made with long straps of jade or silk. Basically, ip-yeong was a practical chin strap to hold the gat tight on the head. Commoners made ip-yeong by folding cotton cloth or fine gauze. However, as available materials became diversified, people used cloth, jade, agate, amber, coral, gold medallion, rock crystal and bamboo as well, juk-yeong, which was made with bamboo, became popular especially when Heungsun Daewon-gun, the father of King Kojong, decreed that people wear simple clothes. Most records concerning ip-yeong in Chosun-wangjo-shillok, the authentic record of the Joseon Dynasty, are related to forbidding a sumptuous life. The book also suggests that ip-yeong was bestowed by the king or was offered to foreign diplomats as gifts. Ip-yeong doesn't seem to be a unique system for Korea. Based on portraits or paintings where ip-yeong can be found, it seems to have been widely used during the Yuan dynasty and the Goryeo dynasty. The system disappeared in China as the Ming Dynasty was established, but it remained in use in the Korean Goryeo dynasty and through the Joseon. Literature suggests that the same materials were used for ip-yeong from the beginning of Joseon to the end of the dynasty. Guyeongja refers to a ring that connects an ipja to a chin strap. Guyeongja made with silver, bronze and jade still remain. In gungjungbalgi, the royal court inventory book, records of imogarye can be found (1882), where ten pairs of pure gold strap rings and ten pairs of gold-plated ones were used for a royal wedding.

• 한국해양학회지
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• v.15 no.2
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• pp.123-128
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• 1980

Variabilities of tidal range at Inchon were described based on observed values. Relationships between tidal ranges and harmonic costants of tide were also examined. Fortnightly variation is predominant and its range is 571.3cm. Mean of maximum spring range(ΔH/sun max/) is 887.2cm and that of minimum neap range(ΔH/sun min/) is 315.9cm. Mean tidal range(ΔH) is 634.3cm. Diurnal inequality is shown about 141cm on an average and monthly inequality is also shown about 100cm. Yearly inquality appears with a range of about 35cm, maxima in March and September, and minima in June and December. There may exist 18 1 years periodicity with a range of about 45cm. There are some relationships between ridal ranges and amplitudes of M$\_$2/ and S$\_$2/, such as ΔH=2.172 H$\_$m/, ΔH$\_$max/=3.043 H$\_$m/, ΔH$\_$min/=1.071 H$\_$m/, ΔH$\_$max/=2.198 (H$\_$m/ + H$\_$s/), and ΔH$\_$min/=1.740 (H$\_$m/ - H$\_$s/).