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Sequence and Characterization of the Genomic Clone of the FVFD16 and FVFD30 Gene Isolated from Flammulina velutipes (팽이버섯에서 분리된 FVFD16과 FVFD30 유전자의 게놈클론의 염기서열 및 특성)

  • Kim, Dool-Yi;Azuma, Tomo-Nori
    • The Korean Journal of Mycology
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    • v.28 no.1
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    • pp.26-31
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    • 2000
  • We isolated genomic clone of FVFD16 and FVFD30 gene specifically expressed during fruit body formation of Flammulina velutipes [(Curt: Fr.) Sing] and determinated the sequences. The FVFD16 gene is including two introns in open reading frame, and FVFD30 gene is including four introns. The introns were matched GT/AG rule. The FVFD16 and FVFD30 genes contained CAAT box with similarity arrange and TATA box. CT-rich region was presented before the transcription start point. FVFD30 gene is investigated that expected the most activity of CCACC arrange. The result of FVFD16 gene analysis showed 80% homology by cDNA clone that is gene family. From the results of genomic southern blot analysis, we presumed more than two copy number gene family of FVFD16 and FVFD30 gene.

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Molecular Divergences of 16S rRNA and rpoB Gene in Marine Isolates of the Order Oscillatoriales (Cyanobacteria) (남조세균 흔들말목(Cyanobacteria, Oscillatoriales) 해양 균주의 16S rRNA와 rpoB 유전자 변이)

  • Cheon, Ju-Yong;Lee, Min-Ah;Ki, Jang-Seu
    • Korean Journal of Microbiology
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    • v.48 no.4
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    • pp.319-324
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    • 2012
  • In this study, we investigated molecular divergences and phylogenetic characteristics of the 16S ribosomal RNA (rRNA) and RNA polymerase beta subunit (rpoB) gene sequences from the order Oscillatoriales (Cyanobacteria). The rpoB of Oscillatoriales showed higher genetic divergence when compared with those of 16S rRNA (p-distance: rpoB=0.270, 16S=0.109), and these differences were statistically significant (Student t-test, p<0.001). Phylogenetic trees of 16S rRNA and rpoB were generally compatible; however, rpoB tree clearly separated the compared Oscillatoriales taxa, with higher phylogenetic resolution. In addition, parsimony analyses showed that rpoB gene evolved 2.40-fold faster than 16S rRNA. These results suggest that the rpoB is a useful gene for the molecular phylogenetics and species discrimination in the order Oscillatoriales.

1$\times$16 DMUX Using Holographic Volume Gratings (홀로그래픽 부피격자를 이용한 1$\times$16 DMUX)

  • Lee, Kwon-Yeon;An, Jun-Won;Kim, Nam;Lee, Hyun-Jae;Seo, Wan-Seok
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.37 no.5
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    • pp.31-38
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    • 2000
  • We propose a new photorefractive demultiplexer(DMUX) which can select the 16 channel signal in WDM optical communication system using the wavelength selectivity and the demultiplexing property through multiple recording of holographic volume grating in photorefractive Fe-LiNbO$_{3}$ crystal. For the multiple writing of the 16 channels having uniform diffraction efficiency, the rotation multiplexing technique and the exposure time schedule are used. Designed DMUX has the 16 channels with 0.5nm spacing between 670nm and 677.5nm and the bandwidth of 0.16nm. From the experimental results, the diffraction efficiency of each channel is 8.3 $\pm$0.62%, the optical loss from fresnel reflection and absorption on the crystal is 0.4cm-1, the 3㏈ bandwidth is 0.16 $\pm$0.005nm and the channel spacing is 0.46~0.5nm.

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Performance of IEEE 802.16j using Cooperative Relaying in Correlated Shadow Fading (음영감쇄 환경에서의 IEEE 802.16j 상호 협조 중계 방식 성능 평가)

  • Ha, Dong-Ju;Kim, Suk-Chan;Park, Dong-Chan;Kim, Young-Il
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.44 no.5
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    • pp.47-53
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    • 2007
  • The purpose of IEEE 802.16j is to extend coverage and to enhance throughput by using relay station additionally to conventional IEEE 802.16e . The cellular system experiences performance degradation at the cell edge due to pathloss, shadow and multipath fading. We can get advantage of spatial diversity gain by using relays more than two cooperatively in the IEEE 802.16j system. Cooperative relaying using space-time code provides better performance under multipath fading and has more robustness against the shadow fading than single relaying. In this paper, we investigate the performance of IEEE 802.16j using cooperative relaying by link level simulation. We also show that the cooperative relaying system achieves better performance than the conventional single relaying system. We apply realistic shadow model considering correlations between shadow fadings of different relaying paths. It is shown that the performance of the system depends highly on the spatial location of relay stations.

The expression of human Spt16 is associated with cell proliferation (인간 Spt16 단백질 발현과 세포 증식 사이의 연관성에 관한 연구)

  • Gwak, Jung-Sug;Cho, Mun-Ju;Ryu, Min-Jung;Oh, Sang-Taek
    • Journal of Life Science
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    • v.17 no.3 s.83
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    • pp.381-385
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    • 2007
  • Facilitates chromatin transcription (FACT) is a chromatin-specific elongation factor required for transcription of chromatin templates in vivo and in vitro. FACT consists of human homologue of the Saccharomyces cerevisiae Spt16/Cdc68 protein (hSpt16) and the high mobility group-1-like protein structure-specific recognition protein-1 (SSRP-1). Here we show that the protein level of hSpt16 is massively down-regulated in quiescent T98C cells using both immunofluorescence and western blot analysis. In contrast, we observe high level of the hspt16 expression in the proliferative T98G cells. Interestingly, the expression of SSRP-1 is not altered in both quiescent and proliferative states. Taken together, our findings implicate that the expression of hSpt16 is associated with the proliferative state and can be used as a proliferation marker.

Theoretical investigation for the molecular structure and Charge transport property analysis of C16H16O3 as a candidate of liquid-crystal (액정 후보 물질로서 C16H16O3의 분자구조 및 전하이동성 특성분석에 관한 연구)

  • Park, Hye-Min;Kim, Seung-Joon
    • Analytical Science and Technology
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    • v.20 no.1
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    • pp.61-69
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    • 2007
  • The geometrical parameters, total and relative energies, vibrational frequencies, the HOMO-LUMO energy gap, and reorganization energies for the neutral molecule, anion, and cation of $C_{16}H_{16}O_3$ have been determined using density functional method (DFT). The highest level of theory employed in this study is $B3LYP/6-311G^{**}$. Harmonic vibrational frequencies were determined at the $B3LYP/6-311G^{**}$ level of theory. All positive vibrational frequencies were obtained to confirm minimum structures. The HOMO-LUMO energy gap and reorganization energies were calculated to predict the charge transport property of liquid-crystal.

A Mobility Supporting MAC Protocol to Improve Handover Performance in IEEE 802.16j Multi-hop Relay Networks (IEEE 802.16j 멀티 홉 릴레이 네트워크에서의 핸드오버 성능 향상을 위한 이동성 지원 MAC 프로토콜)

  • Yang, Hyeon-Chae;Lee, Mee-Jeong
    • Journal of KIISE:Information Networking
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    • v.35 no.5
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    • pp.437-452
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    • 2008
  • IEEE 802.16j MR networks introduce RSs (Relay Stations) within the IEEE 802.16 system in order to enhance the data throughput and extend the coverage. However, the current standardization defines that the BS (Base Station) controls MS's (Mobile Station's) handover, not only it induces the large signaling overhead but also handover latency could increase. In this paper, we propose a handover protocol in the MR networks where the high capability RSs that can process the MS's control functions are deployed. First, we classify the handover scenarios for the MR networks with the high capability RSs. Then, we define the MAC handover procedure, corresponding MAC management messages and the transmission routes for the proposed messages so that an 802.16e MS can perform seamless handover without noticing it is attached to an MR network. The simulation results show that the proposed handover protocol not only reduces the MAC management message overhead transferred through the wireless links compared to IEEE 802.16j/D1, which is the current standard for MR networks, but also performs the rapider and more secure MS handover than IEEE 802.16e and IEEE 802.16j/D1.

Studies on the Triglyceride Composition of Some Vegetable Oils -II. Triglyceride Composition of Corn Oil- (식물유(植物油)의 Triglyceride 조성(組成)에 관한 연구(硏究) -제(第) 2 보(報) : 옥수수유(油)의 triglyceride 조성(組成)-)

  • Choi, Su-An;Park, Yeung-Ho
    • Korean Journal of Food Science and Technology
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    • v.14 no.3
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    • pp.226-231
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    • 1982
  • In order to define triglyceride compositions in fat and oil triglycerides were separated by thin layer chromatography (TLC) from corn oil, and the separated triglycerides were graduated according to each partition number(PN) by high performance liquid chromatography (HPLC) using column of ${\mu}-Bondapack\;C_{18}$ and each graduation was graduated again according to acylcarbon number by gas liquid chromatography(GLC). Fatty acid compositions were analyzed by GLC after their graduation were methylated in according to PN. The triglyceride compositions were estimated by synthesizing the above three results. The estimated triglycerides consisted of 36 kinds in corn oil. The major triglyceride compositions of sample oil were as follows: 21.5%$(C_{18:2},\;C_{18:2},\;C_{18:1})$, 17.4%$(C_{18:1},\;C_{18:2},\;C_{18:1})$, 15.4%$(C_{18:1},\;C_{18:2},\;C_{16:0})$, 11.1%$(C_{16:0},\;C_{18:2},\;C_{18:2})$, 9.0%$(C_{18:1},\;C_{18:1},\;C_{18:1})$, 8.0%$(C_{18:2},\;C_18:2},\;C_{18:2})$, 5.7%$(C_{18:1},\;C_{18:1},\;C_{16:0})$, 2.2%$(C_{16:0},\;C_{16:0},\;C_{18:2})$, 1.6%$(C_{18:2},\;C_{18:2},\;C_{18:2})$, 1.1%$C_{18:2},\;C_{18:0},\;C_{16:0})$, 1.1%$(C_{16:0},\;C_{16:0},\;C_{18:1})$.

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A study on the analyzing of uncertainty for actual evapotranspiration: flux tower, satellite-based and reanalysis based dataset (실제증발산 자료의 불확실성 파악에 관한 연구: flux tower, 인공위성 및 재분석자료)

  • Baik, Jongjin;Jeong, Jaehwan;Park, Jongmin;Choi, Minha
    • Journal of Korea Water Resources Association
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    • v.52 no.1
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    • pp.11-19
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    • 2019
  • In this study, the actual evapotranspiration products of Global Land Data Assimilation System (GLDAS), Global Land Evaporation Amsterdam Model (GLEAM) and MOD16, which are satellite- and reanalysis-based dataset, were validated at the flux tower sites (i.e., CFK and SMK) managed by Korea Institute of Hydrological Survey, and the uncertainty and correlation analysis were conducted using Triple Collocation (TC) method. The result of validation with the flux tower showed better agreement in the order of GLEAM> GLDAS>MOD16. At the result of three combinations (S1: flux tower vs. GLDAS vs. MOD16, S2: flux tower vs. GLDAS vs. GLEAM, S3: flux tower vs. GLEAM vs. MOD16), the order of best to worst is GLEAM, GLDAS, MOD16, and flux tower for CFK (GLDAS> GLEAM>MOD16>flux tower for SMK). Since the error variance and correlation coefficients of the flux tower show relatively worse performance in TC analysis than the other products, By applying TC method to three products (GLDAS vs. GLEAM vs. MOD16), the uncertainty of each dataset were evaluated at the Korean Peninsula, As a results, the GLDAS and GLEAM performed reasonable performance (low error variance and high correlation coefficient), whereas results of MOD16 showed high error variance and low correlation coefficient at the cropland.