• 한국식품영양과학회지
• /
• 제41권12호
• /
• pp.1758-1763
• /
• 2012

본 연구의 목적은 곰취(Ligularia fischeri)와 한대리곰취(Ligularia fischeri var. spicifoprmis) 정유의 향취 및 화학성분을 비교하는데 있다. 곰취와 한대리곰취의 정유 함량은 각각 0.12%와 0.04%였으며, 역치수준은 각각 0.01%와 0.1%였다. 향수개발전문가에 의해 평가된 곰취 정유의 향취는 citrus, coniferous, green, oily, smoky, wet하였던 반면 한대리곰취 정유의 향취는 balsam, earthy, oily, wet하여 매우 상이하였다. 곰취 정유에는 총 19종의 화학성분이 검출되었는데 탄화수소가 14종(75%), 알코올이 4종(20%), 아세테이트가 1종(5%)이었으며, 주된 화학성분은 L-${\beta}$-pinene(36.02%), D-limonene(25.64%), ${\alpha}$-pinene(24.85%), ${\beta}$-phellandrene(5.39%), 3-carene(3.77%), ${\alpha}$-phellandrene(2.05%)이었다. 한대리곰취 정유에는 총 25종의 화학성분이 검출되었는데 탄화수소 17종(68%), 알코올 4종(16%), 아세테이트 3종(12%), 질소화합물 1종(4%)이었으며, 주된 화학성분은 D-limonene(39.80%), L-${\beta}$-pinene(35.43%) ${\alpha}$-pinene(11.94%), 3-carene(3.70%), ${\beta}$-cubebene(3.51%), ${\beta}$-phellandrene(2.38%)이었다. 한대리곰취 정유에서는 ${\rho}$-cymene, ${\gamma}$-muurolene, ${\gamma}$-cadinene, germacrene D, ingol 12-acetate, butyl 9,12,15-octadecatriene과 nimorazole이 미량 검출되었으나, 곰취 정유에서는 이러한 화학성분들이 검출되지 않았다. 본 연구의 결과 각각 독특한 향취를 갖는 곰취와 한대리곰취 정유는 향장산업제품 개발을 위한 소재로 활용될 수 있을 것이라 판단된다.

• 여성건강간호학회지
• /
• 제15권4호
• /
• pp.270-279
• /
• 2009

Purpose: This study was done to investigate the level of breastfeeding satisfaction, the relationship among knowledge about breastfeeding, attitude toward breastfeeding, self-efficacy of breastfeeding, intention of breastfeeding, and perceived social encouragement of breastfeeding and the factors influencing breastfeeding satisfaction in mothers 3~6 months after birth. Methods: For this descriptive research, 209 mothers who had been breastfeeding their babies agreed to participate in this study. Data on mother's satisfaction' knowledge, attitude, self-efficacy, intention, and perceived social encouragement regarding breastfeeding were collected by closed-ended questionnaires on September 14, 2007 at a contest for healthy breast-fed babies. Results: The major findings of this study were as follows: First, there were significant differences in the breastfeeding satisfaction level according to planned length of breastfeeding (F=10.43, p<.001). Second, there was a significant relationship between breastfeeding satisfaction and knowledge about breastfeeding (r=.37, p<.001), attitude toward breastfeeding (r=.62, p<.001), self-efficacy of breastfeeding (r=.66, p<.001), intention of breast feeding (r=.40, p<.001), and perceived social encouragement of breastfeeding (r=.32, p<.001). Finally, the strongest factor affecting mothers' breastfeeding satisfaction was attitude toward breastfeeding ($\beta=.38$). Other factors included self-efficacy of breastfeeding ($\beta=.36$), knowledge about breastfeeding ($\beta=.14$), perceived social encouragement of breastfeeding ($\beta=.10$), and planned length of breastfeeding ($\beta=.10$). Conclusion: This study suggests that nurses should help mothers improve breastfeeding satisfaction through promotion of knowledge about, attitudes toward, and self-efficacy of breastfeeding.

• 한국미생물·생명공학회지
• /
• 제14권3호
• /
• pp.219-223
• /
• 1986

사람 선유아세포 인터페론의 정제에 사용되는 단 clone성 항체생산 세포주를 조작하기 위하여 BA-LB/C mouse의 복강과 꼬리정맥을 통하여 HuIFN-$\beta$를 면역화시키고 그 비장세포(spleen cells) 와 NS-O 세포주를 세포융합 시켰다. 융합된 1300 hybrids를 ELISA방법으로 선별하고 soft agarose 방법과 limiting dilution방법으로 subcloning하여 높은 항체를 생성하는 것으로 판명된 11 hybrids를 재선별 하였다. 재선별된 11 hybrids 각각의 항체형 (Ig type)을 조사하고 최종 Protein A-sepharose와 친화성이 높은 IgG 2a/형의 clone # 4-1-19와 clone # 551-4-1을 선별하여 배양된 세포를 각각 nude(nu/nu) mouse 및 BALB/c mouse 복강에 접종배양 하였다. 이들 mouse복강액으로 부터 얻은 ascites fluid를 protein A-sepharose를 이용한 affinity column분획으로 항체를 정제하였으며 ascites fluid $m{\ell}$당 약 4mg의 정제된 항체를 얻을 수 있었고 SDS-polyacrylamide gel상에서 전기영동 시킨 결과 분자량 14-16만 dalton으로 추정되는 항체를 확인할 수 있었다.

• 임상간호연구
• /
• 제23권3호
• /
• pp.341-349
• /
• 2017

Purpose: Focusing manner is very powerful mind-body self-help and self-awareness competence. So focusing manner may affect nurses' emotional labor and nursing performance, but few are known about it to date. The purpose of this study was to identify the degree of focusing manner, emotional labor, nursing performance of clinical nurses, and the factors that affect nursing performance. Methods: In order to collect data, structured questionnaires were administered to 212 nurses who worked at 2 university hospitals located in D city. Data were analyzed by t-test, ANOVA, correlation and multiple regression using SPSS/WIN 20.0. Results: Nursing performance had positive relationship with focusing manner (r=.45, p<.001), and negative relationship with emotional labor (r=-.25, p<.001). And the most prediction factor influencing nursing performance was focusing manner (${\beta}=.41$, p<.001) and the other factors were age (${\beta}=.30$, p=.002), and emotional labor (${\beta}=.14$, p=.012). The total variance was 37.1% by those predictors (F=25.87, p<.001). Conclusion: Based on the results of this study, it is necessary to develop and educational program on focusing manner-oriented psychotherapy in order to improve the nursing performance. Also, the management system for controlling emotional labor needs to be set up for nurses in clinical settings.

• Preventive Nutrition and Food Science
• /
• 제17권1호
• /
• pp.87-91
• /
• 2012

Three oat cultivars and one oat breeding line were evaluated for chemical, hydration and pasting properties. Protein, starch and ${\beta}$-glucan levels ranged 11.13~14.37, 56.37~64.86 and 3.44~4.76%, respectively. The oat cultivars Daeyang and Seonyang contained higher ${\beta}$-glucan levels of 4.76 and 4.35%. The Daeyang variety had a higher water absorption index (WAI) of 2.83~3.35 (g/g), but a lower water solubility index (WSI) of 8.67~11.08%. Daeyang and Seonyang cultivars showed higher peak and trough viscosity, but lower breakdown and setback, indicating that they easily swell, and thus could possibly provide the desirable viscosity of an oat product. The ${\beta}$-glucan levels were correlated positively with WAI, peak and trough viscosity, and negatively to WSI, breakdown and setback viscosity.

• 한국보건간호학회지
• /
• 제29권3호
• /
• pp.488-502
• /
• 2015

Purpose: This study was conducted to determine the neighborhood environmental factors associated with physical activity among adults living in rural communities. Methods: A cross-sectional descriptive survey was conducted with a convenience sample of 201 adults living in three Ri in Y-city, Gyeonggi-do. Data were collected from face-to-face interview by trained interviewers and were analyzed using a zero-inflated negative binominal regression model. Results: Participants reported engaged in moderate or vigorous physical activity was 76.1%; 10.5% of participants reported that they met moderate physical activity recommendations and 14.5% of participants reported that they met vigorous physical activity recommendations. Zero-inflated negative binominal regression analysis showed association of increasing days of physical activity with social cohesion (${\beta}=.130$, p=.005), social network (${\beta}=-.096$, p=.003), and safety for crime (${\beta}=-.151$, p=.036), and no days of physical activity was associated with no attainment of education and marginally associated with increasing BMI. Conclusion: Neighborhood environmental factors including social cohesion, social network, and crime for safety were significantly associated with physical activity of rural adults. Community health nurses should expand an approach for individual behavior change to incorporate rural adults' specific neighborhood environmental factors into physical activity interventions.

• 한국직업건강간호학회지
• /
• 제28권1호
• /
• pp.36-43
• /
• 2019

Purpose: The purposes of this study were to investigate sleep quality and the related factors among clinical nurses. Methods: Data were collected from 208 nurses in general hospitals through self-report. Pittsburgh Sleep Quality Index (PSQI), Korean Epworth Sleepiness Scale (KESS), Insomnia Severity Index (ISS) were used. The data were analyzed t-test, ANOVA, $Scheff{\acute{e}}$ test, and multiple regression using the SPSS 20.0 program. Results: The mean scores of the subjects were $7.58{\pm}3.61$ on daytime sleepiness, $9.93{\pm}6.22$ on insomnia, and $7.29{\pm}3.05$ on sleep quality. The sleep quality in clinical nurses significantly influenced by insomnia (${\beta}=.55$, t=8.14, p<.001), daytime sleepiness (${\beta}=.16$, t=2.83, p=.005), and work satisfaction (${\beta}=-.15$, t=-2.35, p=.020). The three variables explained 55.9% of variance of sleep quality (F=10.73, p<.001). Conclusion: Sleep quality was influenced by daytime sleepiness, insomnia, and job satisfaction. To improve sleep quality, hospitals managers or leaders need to provide sleep promotion programs for preventing daytime sleepiness and insomnia.

• 한국식품영양과학회지
• /
• 제38권4호
• /
• pp.525-528
• /
• 2009

본 연구는 국내에서 재배되고 있는 포도씨 14종에 대한 식물성 스테롤의 함량과 지방산 조성을 분석하였다. 포도씨의 식물성 스테롤의 함량은 그 품종별로 차이가 있었으나 14품종의 포도씨 모두 $\beta$-sitosterol(64.9$\sim$119.3 mg/100 g)의 함량이 가장 높게 나타났다. 지방산 조성의 비율은 palmitic(16:0, 6.8$\sim$16.8%), palmitoleic(16:1, 0.1$\sim$0.2%), stearic(18:0, 4.7$\sim$5.2%), oleic(18:1, 16.6$\sim$26.1%), linoleic(18:2, 16.6$\sim$20.1%), linolenic acid(18:3, 0.3$\sim$0.5%) 및 arachidic acid(20:0, 0.3$\sim$0.5%)로 분석되었다. 국내에서 재배되고 있는 포도씨 14종의 식물성 스테롤과 지방산의 조성은 유사하였으나 그 함량에는 차이가 있었다. 14품종의 포도씨 중 국내에서 가장 많이 경작되고 있는 Campbell early는 식물성 스테롤의 함량과 지방산 조성에 있어서 비교적 우수한 함량을 보였다. 따라서 Campbell early를 가공함으로써 포도씨유 등과 같은 우수한 품질의 가공품 생산이 가능할 것으로 기대된다.

• 대한의생명과학회지
• /
• 제14권3호
• /
• pp.131-137
• /
• 2008

Adipogenesis is a complex sequence of events that culminates in the differentiation of fibroblast-like preadipocytes into specialized lipid-filled adipocytes and also involves a cascade of expression of many transcription factors such as peroxisome proliferator-activated receptor ${\gamma}(PPAR{\gamma})$ and CCAAT/enhancer-binding proteins (C/EBPs). $PPAR{\gamma}$ and C/EBPs transcriptionally transactivate adipocyte specific genes, including fatty acid transport protein (FAT/CD36) and leptin. To determine whether $17{\beta}$-estradiol modulates $C/EBP{\alpha}$ actions on adipogenesis in high fat diet-fed female ovariectomized (OVX) C57BL/6 mice, mice were treated with $17{\beta}$-estradiol for 7 days and the effects of $17{\beta}$-estradiol on adipose tissue mass and expression of adipocyte specific gene as well as $C/EBP{\alpha}$ were measured. Compared to vehicle-treated OVX control mice, OVX mice treated with $17{\beta}$-estradiol for 7 days had lower adipose tissue weights that were similar to weights in high fat diet-fed sham-operated (Sham) mice. OVX mice showed the increased expression of $C/EBP{\alpha}$ mRNA compared with Sham mice. However, $17{\beta}$-estradiol treatment in OVX mice inhibited OVX induced-$C/EBP{\alpha}$ activation, indicating that $17{\beta}$-estradiol may act as an inhibitor of $C/EBP{\alpha}$ action. Moreover, $17{\beta}$-estradiol decreased mRNA levels of adipocyte marker genes, such as lipoprotein lipase, FAT/CD36 and leptin, to levels in Sham mice. These results suggest that down-regulation of adipogenesis by $17{\beta}$-estradiol may be due to reduced adipose $C/EBP{\alpha}$ activities in female OVX C57BL/6 mice.

• Journal of Radiation Protection and Research
• /
• 제23권2호
• /
• pp.83-88
• /
• 1998

키토산은 키틴의 탈아세틸화반응을 통해서 얻어진 유전적 독성이 없는 천연착화제로써 방사성동위원소 혹은 중금속 이온의 제거제 및 체내 흡수 억제제로 알려져 왔다. 본 연구에서는 분자량이 다른 C-14 chitosan을 정맥투여 한 후 C-14 chitosan 분자량별 마우스 체내 대사과정을 알아보고자 하였다 ICR계 웅성 마우스(8-10주령, 체중 30-35g)를 사용하였다. C-14 chitosan은 증류수로 희석한 다음, 꼬리정맥을 통해 정맥 투여하였다. C-14 chitosan 투여후 6시간, 1일, 3일, 5일, 7일째 마우스를 희생시켜 혈액, 간, 신장, 비장, 폐, 근육, 고환, 오줌을 채취하였으며, 각각의 ${\beta}$-방사능을 측정하여 상대농도를 구하였다. 대부분의 C-14 chitosan이 6시간째에 오줌을 통해 체외 배출되었고, 체내대사과정은 분자량이 서로 다름에도 불구하고 비슷하였다. 간, 신장, 비장등에서 높은 방사능을 나타내었다. 조직간의 상대농도는 6시간째에 증가하다가 서서히 감소함을 알 수 있었다. 결론적으로 정맥 투여한 키토산은 분자량에 상관없이 대부분 오줌을 통해 체외배출 되고, 체내 장기중의 대사과정은 비슷하게 나타났다.