• Journal of the Korean Society of Food Science and Nutrition
• /
• v.41 no.12
• /
• pp.1758-1763
• /
• 2012

This study was conducted to compare fragrance and volatile chemicals of essential oils in Gom-chewi (Ligularia fischeri) and Handaeri Gom-chewi (Ligularia fischeri var. spicifoprmis). Essential oils were extracted by steam distillation of leaves of Gom-chewi (GC) and Handaeri Gom-chewi (HGC), after which samples were collected by solid-phase micro extraction and the compositions of the essential oils were analyzed by gas chromatography-mass spectrometry (GC-MS). The yields of the essential oils in GC and HGC were 0.12% and 0.04%, respectively, and the threshold levels of the essential oils in GC and HGC were 0.01% and 0.1%, respectively. There were 19 constituents of the essential oil of Gom-chewi: 14 carbohydrates, 4 alcohols, and 1 acetate, and the major constituents were L-${\beta}$-pinene (36.02%), D-limonene (25.64%), ${\alpha}$-pinene (24.85%) and ${\beta}$-phellandrene (5.39%). In the essential oil of HGC, 25 constituents were identified: 17 carbohydrates, 4 alcohols, 3 acetates, and 1 N-containing compound, and the major constituents of HGC were D-limonene (39.74%), L-${\beta}$-pinene (35.43%) and ${\alpha}$-pinene (11.94%). The minor constituents of HGC were ${\rho}$-cymene, ${\gamma}$-muurolene, ${\gamma}$-cadinene, germacrene D, ingol 12-acetate and butyl 9,12,15-octadecatriene and nimorazole were not identified in the GC essential oil. Overall, the results showed that the fragrance and chemical compositions of essential oils in GC and HGC differed, suggesting that both essential oils could be used for the development of perfumery products.

• Women's Health Nursing
• /
• v.15 no.4
• /
• pp.270-279
• /
• 2009

Purpose: This study was done to investigate the level of breastfeeding satisfaction, the relationship among knowledge about breastfeeding, attitude toward breastfeeding, self-efficacy of breastfeeding, intention of breastfeeding, and perceived social encouragement of breastfeeding and the factors influencing breastfeeding satisfaction in mothers 3~6 months after birth. Methods: For this descriptive research, 209 mothers who had been breastfeeding their babies agreed to participate in this study. Data on mother's satisfaction' knowledge, attitude, self-efficacy, intention, and perceived social encouragement regarding breastfeeding were collected by closed-ended questionnaires on September 14, 2007 at a contest for healthy breast-fed babies. Results: The major findings of this study were as follows: First, there were significant differences in the breastfeeding satisfaction level according to planned length of breastfeeding (F=10.43, p<.001). Second, there was a significant relationship between breastfeeding satisfaction and knowledge about breastfeeding (r=.37, p<.001), attitude toward breastfeeding (r=.62, p<.001), self-efficacy of breastfeeding (r=.66, p<.001), intention of breast feeding (r=.40, p<.001), and perceived social encouragement of breastfeeding (r=.32, p<.001). Finally, the strongest factor affecting mothers' breastfeeding satisfaction was attitude toward breastfeeding ($\beta=.38$). Other factors included self-efficacy of breastfeeding ($\beta=.36$), knowledge about breastfeeding ($\beta=.14$), perceived social encouragement of breastfeeding ($\beta=.10$), and planned length of breastfeeding ($\beta=.10$). Conclusion: This study suggests that nurses should help mothers improve breastfeeding satisfaction through promotion of knowledge about, attitudes toward, and self-efficacy of breastfeeding.

• Microbiology and Biotechnology Letters
• /
• v.14 no.3
• /
• pp.219-223
• /
• 1986

In order to preparr the hybridoma cells which produce a monoclonal antibody to human fibroblast interferon(Hu IFN-$\beta$), spleen cells from BALB/cmice immunized with the purified Hu IFN-$\beta$ were fused with NS-O cells, a myeloma cell line. Forty hybrids with high titer among 1300 hybrids Isolated by an ELISA screening method were subcloned using the soft agarose cloning and limiting dilution methods, and 11 hybrids were selected. As a result of iso-typing the hybrids using the mouse monoclonal typing kit, two hybridomas were found to produce 1gG 2a type of monoclonal an-tibodies. The ascites fluid from nude mice inoculated intraperitoneally with the above hybridomas was removed and purified using a protein A-Sepharose CL-4B. Monoclonal antibody was proven to have only the heavy and light chains on SDS-polyacrylamide gel electrophoresis.

• Journal of Korean Clinical Nursing Research
• /
• v.23 no.3
• /
• pp.341-349
• /
• 2017

Purpose: Focusing manner is very powerful mind-body self-help and self-awareness competence. So focusing manner may affect nurses' emotional labor and nursing performance, but few are known about it to date. The purpose of this study was to identify the degree of focusing manner, emotional labor, nursing performance of clinical nurses, and the factors that affect nursing performance. Methods: In order to collect data, structured questionnaires were administered to 212 nurses who worked at 2 university hospitals located in D city. Data were analyzed by t-test, ANOVA, correlation and multiple regression using SPSS/WIN 20.0. Results: Nursing performance had positive relationship with focusing manner (r=.45, p<.001), and negative relationship with emotional labor (r=-.25, p<.001). And the most prediction factor influencing nursing performance was focusing manner (${\beta}=.41$, p<.001) and the other factors were age (${\beta}=.30$, p=.002), and emotional labor (${\beta}=.14$, p=.012). The total variance was 37.1% by those predictors (F=25.87, p<.001). Conclusion: Based on the results of this study, it is necessary to develop and educational program on focusing manner-oriented psychotherapy in order to improve the nursing performance. Also, the management system for controlling emotional labor needs to be set up for nurses in clinical settings.

• Preventive Nutrition and Food Science
• /
• v.17 no.1
• /
• pp.87-91
• /
• 2012

Three oat cultivars and one oat breeding line were evaluated for chemical, hydration and pasting properties. Protein, starch and ${\beta}$-glucan levels ranged 11.13~14.37, 56.37~64.86 and 3.44~4.76%, respectively. The oat cultivars Daeyang and Seonyang contained higher ${\beta}$-glucan levels of 4.76 and 4.35%. The Daeyang variety had a higher water absorption index (WAI) of 2.83~3.35 (g/g), but a lower water solubility index (WSI) of 8.67~11.08%. Daeyang and Seonyang cultivars showed higher peak and trough viscosity, but lower breakdown and setback, indicating that they easily swell, and thus could possibly provide the desirable viscosity of an oat product. The ${\beta}$-glucan levels were correlated positively with WAI, peak and trough viscosity, and negatively to WSI, breakdown and setback viscosity.

• Journal of Korean Public Health Nursing
• /
• v.29 no.3
• /
• pp.488-502
• /
• 2015

Purpose: This study was conducted to determine the neighborhood environmental factors associated with physical activity among adults living in rural communities. Methods: A cross-sectional descriptive survey was conducted with a convenience sample of 201 adults living in three Ri in Y-city, Gyeonggi-do. Data were collected from face-to-face interview by trained interviewers and were analyzed using a zero-inflated negative binominal regression model. Results: Participants reported engaged in moderate or vigorous physical activity was 76.1%; 10.5% of participants reported that they met moderate physical activity recommendations and 14.5% of participants reported that they met vigorous physical activity recommendations. Zero-inflated negative binominal regression analysis showed association of increasing days of physical activity with social cohesion (${\beta}=.130$, p=.005), social network (${\beta}=-.096$, p=.003), and safety for crime (${\beta}=-.151$, p=.036), and no days of physical activity was associated with no attainment of education and marginally associated with increasing BMI. Conclusion: Neighborhood environmental factors including social cohesion, social network, and crime for safety were significantly associated with physical activity of rural adults. Community health nurses should expand an approach for individual behavior change to incorporate rural adults' specific neighborhood environmental factors into physical activity interventions.

• Korean Journal of Occupational Health Nursing
• /
• v.28 no.1
• /
• pp.36-43
• /
• 2019

Purpose: The purposes of this study were to investigate sleep quality and the related factors among clinical nurses. Methods: Data were collected from 208 nurses in general hospitals through self-report. Pittsburgh Sleep Quality Index (PSQI), Korean Epworth Sleepiness Scale (KESS), Insomnia Severity Index (ISS) were used. The data were analyzed t-test, ANOVA, $Scheff{\acute{e}}$ test, and multiple regression using the SPSS 20.0 program. Results: The mean scores of the subjects were $7.58{\pm}3.61$ on daytime sleepiness, $9.93{\pm}6.22$ on insomnia, and $7.29{\pm}3.05$ on sleep quality. The sleep quality in clinical nurses significantly influenced by insomnia (${\beta}=.55$, t=8.14, p<.001), daytime sleepiness (${\beta}=.16$, t=2.83, p=.005), and work satisfaction (${\beta}=-.15$, t=-2.35, p=.020). The three variables explained 55.9% of variance of sleep quality (F=10.73, p<.001). Conclusion: Sleep quality was influenced by daytime sleepiness, insomnia, and job satisfaction. To improve sleep quality, hospitals managers or leaders need to provide sleep promotion programs for preventing daytime sleepiness and insomnia.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.4
• /
• pp.525-528
• /
• 2009

In this study, phytosterol content and fatty acid composition of grape seeds from fourteen different cultivars produced in Korea were determined. Although the total phytosterol contents depended upon the cultivars, the major phytosterol was $\beta$-sitosterol (64.9$\sim$119.3 mg/100 g) in all samples. The fatty acid composition of the grape seeds were analyzed as palmitic (16:0, 6.8$\sim$16.8%), palmitoleic (16:1, 0.1$\sim$0.2%), stearic (18:0, 4.7$\sim$5.2%), oleic (18:1, 16.6$\sim$26.1%), linoleic (18:2, 16.6$\sim$20.1%), linolenic acid (18:3, 0.3$\sim$0.5%) and arachidic acid (20:0, 0.3$\sim$0.5%). Campbell early, the mainly produced and consumed cultivar in Korea, contained relatively high phytosterol content compared to other samples and high proportion of unsaturated fatty acids. Therefore, the seeds from Campbell early might be used for functional oil production and as food ingredients.

• Biomedical Science Letters
• /
• v.14 no.3
• /
• pp.131-137
• /
• 2008

Adipogenesis is a complex sequence of events that culminates in the differentiation of fibroblast-like preadipocytes into specialized lipid-filled adipocytes and also involves a cascade of expression of many transcription factors such as peroxisome proliferator-activated receptor ${\gamma}(PPAR{\gamma})$ and CCAAT/enhancer-binding proteins (C/EBPs). $PPAR{\gamma}$ and C/EBPs transcriptionally transactivate adipocyte specific genes, including fatty acid transport protein (FAT/CD36) and leptin. To determine whether $17{\beta}$-estradiol modulates $C/EBP{\alpha}$ actions on adipogenesis in high fat diet-fed female ovariectomized (OVX) C57BL/6 mice, mice were treated with $17{\beta}$-estradiol for 7 days and the effects of $17{\beta}$-estradiol on adipose tissue mass and expression of adipocyte specific gene as well as $C/EBP{\alpha}$ were measured. Compared to vehicle-treated OVX control mice, OVX mice treated with $17{\beta}$-estradiol for 7 days had lower adipose tissue weights that were similar to weights in high fat diet-fed sham-operated (Sham) mice. OVX mice showed the increased expression of $C/EBP{\alpha}$ mRNA compared with Sham mice. However, $17{\beta}$-estradiol treatment in OVX mice inhibited OVX induced-$C/EBP{\alpha}$ activation, indicating that $17{\beta}$-estradiol may act as an inhibitor of $C/EBP{\alpha}$ action. Moreover, $17{\beta}$-estradiol decreased mRNA levels of adipocyte marker genes, such as lipoprotein lipase, FAT/CD36 and leptin, to levels in Sham mice. These results suggest that down-regulation of adipogenesis by $17{\beta}$-estradiol may be due to reduced adipose $C/EBP{\alpha}$ activities in female OVX C57BL/6 mice.

• Journal of Radiation Protection and Research
• /
• v.23 no.2
• /
• pp.83-88
• /
• 1998

Chitosan is a nontoxic natural chealtor which was made by chitin, and reduced a contamination of radiostrontium in animals. In this experiment, A different molecular weight of C-14 chitosan was intravenously administered to mice, and then the distribution of C-14 chitosan in the body was observed. Male mice (8 to 10 weeks, body weight of 30 to 35g) of ICR strain were used. C-14 chitosan was diluted with saline and then given intravenously in mice. After the administration of C-14 chitosan, mice was sacrificed at the 6th hour, 1st, 3rd, 5th, and 7th day. Beta radioactivities in the blood, liver, kidney, liver, muscle, testis, and urine was measured using a liquid scintillation analyzer. Most of the C-14 chitosan was excreted through urine within 6 hours. Biodistribution of C-14 chitosan was similar despite the difference of moleclar weight. Higher distributions of radioactivities were found in the liver, kidney, spleen. The relative concentration in tissue increased for the 6 hours and then decreased. In conclusion, most of C-14 chitosan was excreted through urine despite the difference of molecular weight. and, low molecular weight of C-14 chitosan showed higher distribution than high molecular weight of C-14 chitosan in tissues.