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Changes of Vitamin C and Chlorophyll Contents in Oi-Kimchi with Storage time (저장기간에 따른 오이김치의 비타민 C 및 클로로필 함량변화)

  • Park, Mi-Lan;Lee, Yeon-Jung;Kozukue, Nobuyuki;Han, Jae-Sook;Choi, Suk-Hyun;Huh, Sung-Mee;Han, Gyeong-Phil;Choi, Soo-Keun
    • Journal of the Korean Society of Food Culture
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    • v.19 no.5
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    • pp.566-572
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    • 2004
  • This study was carried out to examine the changes of pH, chlorophyll, chlorophyll patterns and ascorbic acid contents, and sensory evaluation in Oi-Kimhi by the different periods of storage. The contents of chlorophyll a and b were 52.2% and 14.44% at storage 0 days, 20.67% and 16.99% at storage 11 days,0% at storage 14 days, and decreased with storage times increased. But, phephorbide a and b were increased with storage times increased until storage 11 days, and were 28.44% and 1.25% at storage 0 days,42.63% and 15.79% at storage 11 days,0% at storage 12, 14 days. The contents of ascorbic acid were increased until storage 4 days, but afterwards were decreased with storage times increased, and were 2.57mg% at storage 0 days, 5.49mg% and 2.95mg% at storage 11 days, 0% at storage 14 days. Sensory evaluations of appearance, flavor, taste, texture, overall acceptability showed the highest at storage 8 days, and disliked after storage 10 days.

Structural basis of novel TRP14, thioredoxin-related protein that regulates TNE-$\alpha$ signaling pathways

  • Woo, Joo-Rang;Jeong, Woo-Jin;Rhee, Sue-Goo;Ryu, Seong-Eon
    • Proceedings of the Korea Crystallographic Association Conference
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    • 2003.05a
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    • pp.18-18
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    • 2003
  • Thioredoxin (Trx) is a small redox protein that is ubiquitously distributed from achaes to human. In diverse organisms, the protein is involved in various physiological roles by acting as electron donor and regulators of transcription and apoptosis as well as antioxidants. Sequences of Trx within various species are 27~69% identical to that of E. coli and all Trx proteins have the same overall fold, which consists of central five β strands surrounded by four α helices. The N-terminal cysteine in WCGPC motif of Trx is redox sensitive and the motif is highly conserved. Compared with general cysteine, the N-terminal cysteine has low pKa value. The result leads to increased reduction activity of protein. Recently, novel thio.edoxin-related protein (TRP14) was found from rat brain. TRP14 acts as disulfide reductase like Trx1, and its redox potential and pKa are similar to those of Trx1. However, TRP14 takes up electrons from cytosolic thioredoxin reductase (TrxR1), not from the mitochondrial thioredoxin reductase (TrxR2). Biological roles of TES14 were reported to be involved in regulating TNF-α induced signaling pathways in different manner with Trx1. In depletion experiments, depletion of TRP14 increased TNF-α induced phosphorylation and degradation of IκBα more than the depletion Trx1 did. It also facilitated activation of JNK and p38 MAP kinase induced by TNF-α. Unlike Trx1, TRP14 shows neither interaction nor interference with ASK1. Here, we determined three-dimensional crystal structure of TRP14 by MAD method at 1.8Å. The structure reveals that the conserved cis-Pro (Pro90) and active site-W-C-X-X-C motif, which may be involved in substrate recognition similar to Trx1 , are located at the beginning position of strand β4 and helix α2, respectively. The TRP14 structure also shows that surface of TRP14 in the vicinity of the active site, which is surrounded by an extended flexible loop and an additional short a helix, is different from that of Trx1. In addition, the structure exhibits that TRP14 interact with a distinct target proteins compared with Trx1 and the binding may depend mainly on hydrophobic and charge interactions. Consequently, the structure supports biological data that the TRP14 is involved in regulating TNF-α induced signaling pathways in different manner with Trx1.

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Changes of Volatile Compounds in the Pericarp of Chopi (Zanthoxylum piperitum DC.) During Maturation (초피 과피의 성숙정도에 따른 향기성분의 변화)

  • ;;;;;;;;Ken C. Sink
    • Journal of Life Science
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    • v.13 no.2
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    • pp.206-213
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    • 2003
  • This study was conducted to estimate volatile compounds in pericarp of Zanthoxylum piperitum DC (Chopi). Chopi which harvested on lune 2, July 14 and September 11 in 2001 was dried at room temperature for one week. Fifty-two, 47, and 44 volatile compounds were analyzed with GC-MS in pericarp harvested on lune 2, July 14 and September 11, respectively. Eight terpenes including myrcene, ${\gamma}$-terpinene, $\alpha$-terpinolene, $\alpha$-phellandrene and $\beta$-caryophyllene were detected in pericarp harvested on tulle 2 and July 14, but not $\alpha$-phellandrene and $\beta$-caryophyllene in pericarp harvested on September 11. Thirteen alcohols or terpene alcohols including linalool L and citronellol were detected in pericarp harvested on lune 2, and added cis-linallol oxide and piperitol isomer in pericarp harvested on July 14 and September 11. Three aldehydes or terpene aldehydes were not affected by degree of maturation, but citronellal was increased in pericarp harvested on September 11. Five volatile compounds of ketones containing cryptone and piperitone were detected, and their concentration was changed during maturation. Six esters including lavandulyl acetate and $\alpha$-terpinenyl acetate were detected in pericarp harvested on lune 2, and [(E)-6,7-ephoxy-3,7-dimethyl-2-octenyl]ester of acetic acid was added in pericarp harvested on July 14 and September 11. Seven hydrocarbons including $\delta$-cadinene and neopentylidene cyclohexane were detected in pericarp harvested on June 2 and $\alpha$-muurolene was newly added in pericarp harvested on July 14 and September 11. We suggest that kinds and concentration of volatile compounds in pericarp were remarkably different from those in mature stage.

Identification of Quantitative Trait Loci for Resistance to Soybean Cyst Nematode Race 14 (콩시스트 선충 race14에 대한 저항성 유전자좌 구명)

  • Choi, In-Soo;Kim, Yong-Chul
    • Journal of Life Science
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    • v.13 no.4
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    • pp.375-382
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    • 2003
  • A direct and precise explanation of soybean resistance to soybean cyst nematode will be possible only when the individual gene(s) involved in the resistance are tagged. This study was conducted, (1) to identify and localize quantitative trait loci for resistance to soybean cyst nematode race 14 on RAPD map, (2) to identify the magnitude and mode of inheritance for each quantitative trait loci, and (3) to identify the best combinations of quantitative trait loci for resistance to soybean cyst nematode race 14. Thirty markers (29 RAPD and 1 RFLP) showed significant association with resistance to soybean cyst nematode race 14. From MAPMAKER/QTL analysis, we identified two regions (linkage group C-7 and linkage group C-9) for resistance to soybean cyst nematode .ace 14. The first quantitative trait loci that was localized at 6.0 cM from $H06^1$ on linkage group C-7 showed a dominant inheritance mode. However, we can not exclude the possibility of additive inheritance mode. The second quantitative trait loci that was localized between $B15^2$ and $E01^1$ on linkage group C-9 also showed a dominant mode of inheritance. One pair of flanking markers ($H06^1$ and $H06^2$) and B15$^2$ were used for multiple regression analysis. Marker combination that included 2 markers, $B15^2$ and $H06^1$, explained the highest total variance (22.9%) for resistance to soybean cyst nematode race 14. Further localization of genes for resistance to soybean cyst nematode race 14 and examination of interaction between quantitative trait loci will accelerate the exploitation of resistance to soybean cyst nematode.

Isolation, Regeneration and the Maturation Induction of Free-Living Gametophytes of Undaria pinnatifida (Phaeophyceae) (갈조류 미역 유리배우체의 분리, 재생 및 성숙 유도)

  • Yoo, Hyun Il;Kim, Su Hong;Lee, Gi hyun;Ha, Dong Soo;Hwang, Eun Kyoung
    • Korean Journal of Environmental Biology
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    • v.35 no.4
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    • pp.622-630
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    • 2017
  • The induction of regeneration and the maturation of the free-living gametophytes of Undaria pinnatifida were studied at temperatures(5, 10, 15 and $20^{\circ}C$), irradiance (5, 10, 20 and $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$) and photoperiods (14 : 10, 12 : 12 and 10 : 14 h L: D). Female gametophyte fragments were maintained in active regeneration without them reaching sexual maturity under conditions of $15^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D); by contrast, the conditions for male gametophytes were slightly different at $15^{\circ}C$, $5{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 14 : 10 h (L: D). The sexual maturation of female and male gametophytes took place under $5^{\circ}C$, $20-40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D) and $15^{\circ}C$, $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for srain improvement as well as the cross breeding of U. pinnatifida.

RD-14 자연순환 실험에 대한 RELAP5 코드 모사

  • 양채용;조용진;김인구;이석호;이종인
    • Proceedings of the Korean Nuclear Society Conference
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    • 1996.11a
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    • pp.376-380
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    • 1996
  • RD-14은 CANDU형 발전소를 묘사하기 위한 실험장치로서 여러 초기조건에서 자연순환 실험이 수행되었다. 본 연구에서는 RD-14 실험장치의 자연순환 실험을 RELAP5 코드로 모사하여 결과를 비교·평가하였다. 본 연구는 CANDU형 발전소 사고해석의 검증용 코드로서 RELAP5 코드의 적용 타당성을 평가하기 위한 과정으로 수행되었다. 계산결과, RELAP5 코드는 RD-14 실험장치의 자연순환 실험을 잘 예측하고 있음을 보여주고 있어 CANDU형 발전소의 자연순환 평가에 유용하다는 결론을 얻었다.

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Accelerator Mass Spectrometry in Environmental Research (가속기 질량 분광분석법을 이용한 환경 연구)

  • 문창범
    • Journal of Korean Society for Atmospheric Environment
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    • v.16 no.4
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    • pp.421-428
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    • 2000
  • Accelerator Mass Spectrometry (AMS) is presented for the applications to the environmental research. Traditionally the radiocarbon (14C) measurements have been made for the purpose of dating the archaeological and geological samples. 14C measurements using AMS however have become the more useful methods for the study of environmental science such as antropogenic modification of atmosphere and the determination of mixing ratios of fossil and biomass products. Such 14C AMS measurements have been extended to the discovery of new chemical tracers for investigations of sources of excessive contributions of CO2, CO, CH4 and other carbon-bearing molecules in the atmosphere.

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Plans to Improve the Quality of Ready-Mixed Concrete and a Auggestion of Legislating a Concrete Engineer Certification (레미콘의 품질확보 방안 및 콘크리트 기사 자격제도)

  • 박승범
    • Magazine of the Korea Concrete Institute
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    • v.14 no.4
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    • pp.14-21
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    • 2002
  • 국내에서 시공되는 콘크리트 구조물의 대부분은 레미콘(ready-mixed concrete)으로 시공되고 있기 때문에 레미콘의 품질관리(quality control) 및 품질확보(quality security)는 곧 콘크리트 구조물의 품질과 내구성의 확보를 위해 매우 중요한 사항이다. 최근 콘크리트 구조물의 잦은 사고로 인해 콘크리트의 품질문제가 사회문제로 나타나고 있으며 이에 대비한 콘크리트 품질보증 시스템의 확립이 시급한 실정이다. 그러나 레미콘의 품질환경은 원재료의 품질저하, 레미콘 운반시간의 증대 및 기술개발의 미흡 등 주변여건이 좋은 편은 아니며 레미콘 산업도 경쟁시대에 돌입하면서 레미콘 품질이나 기술우위의 시대로 변화되고 있다.(중략)

Interview - 제14회 한용교포장인상

  • (사)한국포장협회
    • The monthly packaging world
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    • s.215
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    • pp.99-105
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    • 2011
  • (사)한국포장협회(회장 윤영욱)는 지난 2월 25일 '포장인의 날'을 맞이해 제14회 한용교포장인상 시상식을 개최했다. 제14회 한용교포장인상 수상자는 장학생 부문 ${\blacktriangle}$ 최우석(연세대학교 패키징학과) ${\blacktriangle}$ 서예슬(용인송담대학 포장코디네이션 전공) ${\blacktriangle}$ 곽주현(순천청암대학 물류패키징과) 학생이 수상했으며, 일반 부문은 수상자를 내지 못했다. 한용교포장인상은 한국포장협회 한용교 명예회장이 희사한 재원으로 포장인들에게 연구의 욕을 높이고 자긍심을 고취, 포장산업을 발전시키고자 지난 1996년 제정됐다. 본 고에서는 제14회 한용교포장인상 수상자들의 활약상을 살펴본다.

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