• Journal of Veterinary Clinics
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• v.12 no.1
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• pp.877-886
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• 1995

The recycling nuclear transplantation(NT) technique has the powerful potential of producing a large number of genetically identical embryos and offsprings from one embryo. Multiple generational cloning by this technique utilizes the NT embryo itself as the donor for the next generation of cloning. In this experiment, we have produced the third generational cloned embryos by recycling NT. Further we examined comparatively the electrofusion rate and in vitro developmental potential in the cloned embryos of the first second and third generations. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulberco's phosphate buffered saline containing 10 % fetal calf serum(FCS) at 47 hours after hCG injection. In the first generation NT, the nuclear donor embryos were synchronized in the phase of Gl/S transition of 32-cell stage. The first and second generation NT embryos developed to 16-cell were used as donor nuclei for second and third generation. The recipient cytoplasms were utilized the oocytes collected at 14 hours after hCG injection, following revoming the nucleus and the first polar body by micromanipulation. The separated blastomeres were injected into the enucleated recipient oocytes by micromanipulation and were fused by electrical stimulation. The electrofusion rate was seen to be 78.0, 88.0 and 90.3 % in the first second and third generation NT rabbit embryos, respectively. The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10 % FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. The in vitro developmental potential to blastocyst stage was significantly(P<0.05) decreased in the third(7.2 %) generation NT embryos compared to the first(53.1 %) and second(16.1 %) generation NT embryos. Following in vitro development to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The mean blastomere numbers and cell cycle numbers of NT embryos during the culture period were significantly(p<0.05) decreased in the second(93.9 cells and 6.55 cylces) and third(81.5 cells and 1.35 cylces) generation, compared to the first(189.9 cells and 7.55 cylces) generation.

• Journal of Korean Society of Forest Science
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• v.3 no.1
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• pp.15-17
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• 1963

1) An experiment was made at the newly constructed glass house of Chun chan Agriculture callege to find out facts about the late-raising of Cortinellus edodes (Berk) Ido et Imai during the winter season. 2) The host timbers used in this experiment were Quercus Serrata Thumb and Quercus crispulo BI.; 5 years old each, the diameter ranging from 6cm to 17cm, dividing them into 12 diameter groups with 10 timbers in each group. The total number of timbess was 240 in two blocks. 3) As the pre-treatment of host timber, the autumn generation in 1962 was inhibited, and before being put into the glass-house, they were stimulated of generation for 3 days after 3 days, water-soaking. 4) The temperature control was made by using two coal stoves and the humidity control by spraying and two full water tanks. During cloudy weather and night, the green house was covered to preserue constant temperature of $5^{\circ}C{\sim}10^{\circ}C$. 5) The result; Quercus Serrata Thumb. with lesser diameter than 9cm showed no generation either in as big timber as one with diameter 11 cm. On the other hand, too big timbers showed low rate of generation; the reason, at a guess, is the too short period of cultivation and insufficient water soaking and inappropriate temperature. 6) The most standard size of host timber was found out be those with diameter 12cm~15cm in Quercus serrata Thumb, and those with diameter 14cm~17cm in Quercus crispula BI. Quercus crispula seemed to have slightly greater efficiency of generation than Quercus Serrata Thumb., but it could not be concluded as "decisive". Lastly, the host timber used in this experiment were old and the rate of generation was low as a whole.

• Journal of the Korea Organic Resources Recycling Association
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• v.14 no.3
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• pp.91-100
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• 2006

In this study, the characterization of unit load generation and discharge from various type stall of piggery was conducted by investigation and analysis of contaminants loading from piggery urine, manure and wastewater. The results are summarized as follows: The unit load generation of filth increases as piggery grow older, but there was not large enough difference among those values of unit load evaluated for various stall types if mean values of each type of stall are considered. The generation amounts of manure and urine were total 4.57kg/head/d of 1.49kg manure/head/d and 3.08kg urine/head/d with consideration of 3 seasons and live weight. The finalized mean unit load generation of filth were estimated at BOD 199.5g/head/d, $COD_{cr}\;413.5g/head/d$, T-N 27.8g/head/d, T-P 5.3g/head/d with consideration of seasons and the type of stalls. The wastewater unit loads discharged from cement type stall were estimated at BOD 31.3g/head/d, $COD_{cr}\;95.6g/head/d$, T-N 8.9g/head/d, T-P가 3.1g/head/d. The sum of manure unit load generation considered with manure collection ratio(80%, 90%) and wastewater unit load was almost similar when compared to the unit load discharged from slurry type stall even though more or less difference were appeared according to each contaminants and parameters.

• Journal of the Korean Institute of Intelligent Systems
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• v.24 no.1
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• pp.40-45
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• 2014

This paper suggests the optimal generation dispatch algorithm for ensuring voltage stability margin considering high wind energy injection. Generally, with wind generation being installed into the power system, we would have to consider several factors such as the voltage stability margin because wind turbine generators are mostly induction machines. If the proportion of wind generation increases in the power system increases this would affect the overall stability of the system including the voltage stability. This paper considers a specific system that is composed of two areas: area 1 and area 2. It is assumed that generation cost in area 1 is relatively higher than that in area 2. From an economic point of view generation in area 1 should be decreased, however, in the stability point of view the generation in area 2 should be decreased. Since the power system is a nonlinear system, it is very difficult to find the optimal solution and the genetic algorithm is adopted to solve the objective function that is composed of a cost function and a function concerned with voltage stability constraints. For the simulations, the New England system was selected. The algorithm is implemented and Python 2.5.

• Journal of Korean Society on Water Environment
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• v.20 no.4
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• pp.333-338
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• 2004

This study was carried out to investigate the generation and discharge characteristics of non-point pollutants from farmlands in Nak-dong river basin. Annual unit generation load of nitrogen and phosphorus by fertilization in the test paddy field was almost similar to those calculated by the fertilization standards of district agricultural technology center, but it was extremely higher in case of the test dry field. By comparing annual total generation load of nutrients from fertilization to the data of fertilizer marketing, the accurate forecasting of generation load of pollutants was achieved by marketing data. The annual total discharge ratio of nutrients through infiltration and overflow from the farmland of the test paddy field were 9.5% and 1.1%, respectively, and those in the test dry field were 22.0% and 0.1%, respectively. The monthly discharge load of nutrients were shown the highest proportioned to the discharge load from lands, but it showed higher in phosphorus, which was caused by the intermittent discharge of phosphorus accumulated in drainage.

• Korean Journal of Human Ecology
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• v.4 no.1
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• pp.13-29
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• 1995

The purposes of this study are to investigate the characteristics of recognitions and attitudes of new generation family on the family life, and to help the family members of the new generation family to be able to adapt to the future family and society with the right senses and attitudes on the family life. Three-hundred married men and women who were less than 35 years old, and who continued marriage for less than five years were selected. The results of this study were as follow; 1) Types of family composition was mostly nuclear family, and got help from their parents in managing household work. 2) Most new generation family was more likely to share equal roles and rights in household affairs, decision making and ownership of real properties. 3) Most new generation family considered the personality and the sense of value as the most important conditions which their spouse must have. And they were affirmative to wives' employment. 4) Most new generation family wanted 2 children. And preferred showed that they did not care much about son preference. 5) Most new generation family showed strong sense of responsibility for taking care of their old parents. However, they showed independency of financial planning for later years. 6) In many ways of life, their recognitions and attitudes were progressive but their were conservative in practical life as the older generations did.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.25 no.5
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• pp.104-109
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• 2011

In this paper, after an acceleration typed ion wind generator which could format strong electric field in air was manufactured and installed, the effects of the electrode configuration and distance of acceleration type ion wind generator with triangle structure on the ion wind generation characteristics were investigated. As a result, the ion wind generator with curvature multipoint electrode could generate higher ion wind velocity and ion wind generation yield than others with multipoint electrode, curvature line electrode, line electrode structure. The ion wind generator with curvature multipoint electrode showed a peak ion wind velocity of 1.33[m/s] at 19.0[kV] and a ion wind generation yield of 0.12[m/Ws] at 15.0[kV].

• Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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• 2004.02a
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• pp.73-81
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• 2004

National Geographic Information Institute (NGII) in Korea, through National Geographic Information System (NGIS) Program, has prepared to generate and disseminate digital elevation data for Korea. This is a pilot research to propose a policy for generation, maintenance, and supply of Korea Digital Elevation Data (KDED). Customer demands for accuracy and resolution of DEM was surveyed through questionnaire. In order to investigate the quality, the technical efficiency and the production cost, a tentative DEM in a small test site was generated based on digital topographic maps (original paper map scale 1 :5,000), analytical plotter, and LIDAR. Accuracy standard for KDED was derived based on source data and generation methods. As results of this research, we recommend uniformly spaced grid model for KDED. Its preferable grid space is 5m in urban and its vicinity; and 10m in field and mountainous area. LIDAR has been valuated as a proper KDED generation method fulfilling customers demand for the accuracy.

• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1427-1433
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• 2019

In this paper, Gas engine was tested for the energy of synthesis gas. As excess air ratio increase 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6 in 1800 rpm and synthesis gas, thermal efficiency generally decrease and power generation was 34 kWm at λ 1.4. And excess air ratio increase 1, 1.1, 1.2, 1.3, 1.4 in power generation 34 kWm, thermal efficiency generally increase 34.2%, 36.9%, 37.2%, 37.4%, 38.1%. Total efficiency through power generation consumes 38.7 kg/h of fuel at 30 kWe load and recovers 57.3% of waste heat by recovering 57.3 kW of waste heat through 32.1% power generation efficiency and heat recovery from cooling water and exhaust gas. The total efficiency was 85.8%.

• Journal of the Korean Chemical Society
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• v.48 no.6
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• pp.583-589
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• 2004

In a stream of aqueous sample, trace arsenic ions were quantitatively coprecipitated and detected in ICP-AES through hydride generation. In was used as a coprecipitating reagent. The precipitate was collected on a filter and dissolved by HCl. The eluted As was sent into the reaction coil to generate hydrides and analyzed by ICP. With optimal conditions, and with a sample of 0.3 mL, an enrichment of 70 was obtained with the sampling speed of 10/hr. When compared with coprecipitation and hydride generation technique, the sensitivity was increased by 7 and 10 times, respectively. The limit of detection limit$(3{\sigma})$ was 0.020 ${\mu}g\;L^{-1}$ and the precision was 7-10%. Separation of $As^{3+}\;and\;As^{5+}$ were possible using citric acid in hydride generation.