• Journal of Marine Life Science
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• v.8 no.2
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• pp.190-195
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• 2023

In this research, the marine medaka Oryzias javanicus underwent a 96 h exposure to two concentrations of the red tide dinoflagellate Karenia mikimotoi (1,000 and 5,000 cells mL^-1), and the temporal variations in biochemical responses related to antioxidant and immunity parameters were assessed in the liver tissue. The study revealed a significant increase in ichthyotoxicity with elevated cell concentrations of K. mikimotoi, especially evident at 96 h in marine medaka exposed to 5,000 cells mL^-1. At 1,000 cells mL^-1 of K. mikimotoi, the opercular respiratory rate showed a significant increase, whereas exposure to 5,000 cells mL^-1 resulted in a lowered rate. The intracellular malondialdehyde content was significantly elevated in response to both cell concentrations at 96 h. Regarding glutathione content, levels were significantly increased by exposure to both cell concentrations. Catalase and superoxide dismutase enzymatic activities experienced an increase at 1,000 cells mL^-1 of K. mikimotoi, while their activities were reduced at 5,000 cells mL^-1 at 96 h. The analysis of two immunity parameters, alternative complement pathway and lysozyme, demonstrated significantly reduced activities in the liver tissue exposed to 5,000 cells mL^-1 of K. mikimotoi. These findings aim to enhance the understanding of K. mikimotoi toxicity in marine fish by offering insights into biochemical responses associated with harmful algal blooms.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.2
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• pp.228-235
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• 2011

An nematophagous fungi Arthrobotrys thaumasia Nema-1 and Pseudomonas putida C-5, which degrade the collagen and gelatin, was isolated from controlled horticultural soils in Seonnam-myun, Sungju-gun, Kyungpook and Woosung-myun, Gongju-shi, Chungnam to develop biological nematode pesticide. When $5,000mL\;L^{-1}$ of A. thaumasia Nema-1 culture was treated to Meloidogyne incognita, the nematicidal activity resulted in 55% at 72 hours after treatment. While the nematicidal activity increased to 65% by treating the culture mixture of $5,000mL\;L^{-1}$ Nema-1 and P. putida C-5 after 72 hours. Furthermore, the nematicidal activity of the mixture containing cinnamon extract $50mg\;L^{-1}$, each $5,000mL\;L^{-1}$ of Nema-1 and C-5 culture was elevated to 89% at 72 hours after treatment, comparing to the result showed 17% and 57% of the nematicidal activity, respectively by the treatment of chemical nemato pesticide Fosthiazate $50mg\;L^{-1}$ and neem oil $2,000mL\;L^{-1}$. These results suggested that the mixture of microorganisms and plant extract were more effective biological nematicide than the case of only microorganism or plant extract for nematode control.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.1
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• pp.23-30
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• 2002

Eveuadies 71' kenaf (Hibiscus cannabinus L.) was grown at five plant densities (1, 2, 3, 4, and 5 plants per hill; 16,000, 32,000, 48,000, 64,000 and 80,000 plants/10a) from April 13 to October 8, 2001 in Jeju island to determine influence of plant number per hill on agronomic characteristics, forage yield and quality. As plant density increased from 1 to 5 plants per hill, the plant height averaged across two harvests increased 176 to 185 cm. Compared with the plant density of two plants per hill, the plant height decreased at higher plant densities (183, 180, and 166 cm for 3, 4 and 5 plants per hill). The highest yield of fresh forage (114.6 MT/ha), dry matter (17.3 MT/ha), crude protein (2.33 MT/ha), and total digestible nutrients (TDN) (9.21 MT/ha) was obtained at 2 plants per hill. As plant density increased from 1 to 5 plants per hill, crude protein, ether extract, nitrogen free extract, and TDN contents increased from 13.1 to 15.4%, 3.0 to 3.7%, 20.1 to 23.2% and 41.1 to 45.4%, respectively, while crude fiber and crude ash contents decreased from 41.2 to 34.9% and 9.4 to 8.2%, respectively.

• Korean Journal of Food Science and Technology
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• v.28 no.6
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• pp.1065-1070
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• 1996

Antimutagenic effect of chitosan hydrolysates was investigated using Salmonella typhimurium reversion assay and SOS chromotest against 3-amino-1-methyl-5H-pyrido[4,3-b]lindole(Trp-P-2), aflatoxin $B_1$, 2-nitrofluorene and 4-nitroquinoline oxide. After partial acid hydrolysis of chitosan, six fraction of different molecular size were obtained by ultrafiltration. Chitosan hydrolysates showed antimutagenic effect of $0{\sim}78%$ on Trp-P-2, $0{\sim}92%$ on aflatoxin $B_1$ and $0{\sim}51%$ on 2-nitrofluorene in Salmonella typhimurium reversion assay. Inhibitory effect in Salmonella typhimurium reversion assay showed the highest at 5% concentration of fraction 6 in Trp-P-2, 10% concentration of fraction 5 in aflatoxin $B_1$ and 5% concentration of fraction 6 on 2-nitrofluorene. In SOS chromotest, chitosan hydrolysates showed anitimutagenic effect of $0{\sim}54%$ on Trp-P-2 and $0{\sim}77%$ on 4-nitroquinoline oxide, These results suggest that high molecular weight fraction of chitosan hydrolysates (MW>30,000) in most effective to inhibit mutagenicity of tested mutagens.

• The Korean Journal of Food And Nutrition
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• v.21 no.1
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• pp.15-21
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• 2008

In this study, the minimum inhibition concentration(MIC), growth inhibition activity, and colony forming inhibitory activity of water soluble propolis against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae and Salmonella enteritidis were tested. The MICs of the water soluble propolis against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, and Salmonella enteritidis were 312.5 ppm, below 156.3 ppm, 625 ppm, 10,000 ppm, above 10,000 ppm, 10,000 ppm, above 10,000 ppm, above 10,000 ppm, 10,000 ppm, and above 10,000 ppm, respectively. The growth inhibition concentrations against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, and Klebsiella pneumoniae were 156.3 ppm, below 156.3 ppm, 625 ppm, 5,000 ppm, 10,000 ppm, 10,000 ppm, 10,000 ppm, 10,000 ppm, and 5,000 ppm, respectively. However, 10,000 ppm did not inhibit the growth of Salmonella enteritidis. Finally, the colony forming inhibitory activities against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, and Salmonella enteritidis were 98.0%, 99.8%, 69.8%, 98.1%, 62.0%, 63.1%, 79.5%, 61.9%, 79.6%, and 0.0%, respectively.

• Journal of Korean society of Dental Hygiene
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• v.3 no.1
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• pp.1-12
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• 2003

The purpose of this study was to verify the reliability of the instrument and to analyze the contents of the humor. This study was conducted from December 16th, 2002 to January 13th, 2003, centering on Busan Dental Hospital & public health service. A total of 193 questionnaires was distributed for the survey. The result were as followings: 1. There were significant differences in humor values concerning age(F=1l.44, p=0.000), marriage(t= -3.556, p=0.000), education(F=14.83, p=0.000), clinical carrier(F=9.99, p=0.000), status(F=5.83, p=0.001), working place(F=7.39, p=0.000), and living parents(F=3.65, p=0.014). Humor values were higher for over-forties, married dental hygienists, public health service worker and no living parents. 2. There were no Significant correlation between stress and the dental hygienist's humor values. 3. There were significant differences in humor values concerning sources of the dental hygienist's humor. Data related "book/mass media"(t=-6.32, p=0.000), "conversation" (t=-12.05, p=0.000) and "daily life"(t=-10.33, p=0.000) are examples of these sources. 4. There were no significant differences in humor values concerning the dental hygienist's humor degree related frequency of used humor. 5. There were significant differences in humor values concerning the type of humor used, "word humor"(t=-7.00, p=0.000), "imitation"(t=2.68, p=0.008), "adequate situation" (t=-8.03, p=0.000), "technical terms"(t=6.65, p=0.000) pertain to this. 6. There were Significant differences in humor values concerning the time of using humor expression, "loose situation"(t=-3.75, p=0.000), "tired situation"(t=4.01, p=0.000), "tense situation"(t=5.37, p=0.000), "adequate situation"(t=-16.03, p=0.000) pertain to this.

• Korean Journal of Community Nutrition
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• v.7 no.2
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• pp.149-155
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• 2002

The purpose of this study was to investigate the consumption pattern of convenience flood products, and related factors among 229 Chungnam College student in Daejeon, using a written questionnaire. Of the students 31.9% were male, and 68.1% female. The results have been summarized as follows: 1) The averages household income ranged from ￦ 1,500,000 to ￦ 2,070,000 a month. Convenience flood intake of lunch was 46%, and of snacks was 31.4%. The frequency of purchasing convenience floods with a manufacturer s date was 6.09 $\pm$ 1.16, with packing conditions was 5.55 $\pm$ 1.21, with ingredients listed was 5.43 $\pm$ 1.23, and with a price was 5.40 $\pm$ 1.16. 2) In comparing household income by convenience food intake, those with household incomes ranging from ￦ 2,000,000 to ￦ 2,500,000 had a frequency of convenience flood intake of 2 to 3 times per month. When the pocket money level increased, the convenience flood intake increased. This study provides information on convenience flood intake.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.11
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• pp.1645-1652
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• 2015

We investigated the anti-wrinkle efficacy of hydrolysate from oyster protein by Protamex and Neutrase for the purpose of finding materials to assist skin health originating from marine organisms. There were about 7.9% free amino acids in the oyster hydrolysate, and contents of urea, taurine, alanine, and glycine were high. Oyster hydrolysate also showed collagenase inhibitory activity and was not toxic to CCD986sk human fibroblast cells. Yield of the fractions according to the molecular weight of oyster hydrolysate was 40% for less than 1,000 Da and 60.4% for less than 5,000 Da, respectively. Antioxidative effect, procollagen production, and matrix metalloproteinase-1 inhibitory activity were highest in 1,000~3,000 Da fractions. We observed that oyster hydrolysate and its less than 5,000 Da fraction are potential functional compounds for skin health and for improving wrinkles.

• Journal of Korean Society for Geospatial Information Science
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• v.6 no.1 s.11
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• pp.117-128
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• 1998

National digital maps (NDM) produced by diverse production methods through various stages are ready to distribute to public. The position accuracy problems in NDM should be inspected and evaluated to guarantee the quality of NDM. The purpose of this study is 1) to find out factors of impeding accuracy by examining the position accuracy of NDM on scales of 1:1,000 and 1:5,000, 2) to form the technical basis of making accurate digital maps and 3) to increase reliability and practical use of NDM. In this study, we found out 1) obstacles of making accurate mM especially in solving horizontal and vertical location accuracy problems and 2) error sources in production methods as well as stages. These results can be contributed to increase accuracy on modifying and upgrading NDM.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.22 no.1
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• pp.29-36
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• 2004

This study describes the development of coordinate transformation tool for transforming the digital map using newly derived transformation parameters which are determined from the data referred to the local geodetic datum and the geocentric datum (ITRF2000) and the distortion modelling derived from collocation method. We prepared 190 common points and used 107 points to calculate 7 transformation parameters. In order to evaluate an accuracy of coordinate transformation, 83 common points were tested. In this study, we used Molodensky-Badekas model to derive the 7 transformation Parameters. An accuracy of 0.22m was obtained applying 7 Parameters transformation and the distortion modelling together. It shows that the accuracy of coordinate transformation is improved 72% against the result of 7 parameters transformation only. We developed the transformation tool, GDKtrans, which can be transformed the digital map of scales 1/50,000, 1/25,000 and 1/5,000. We also analyzed the digital map of l/5,000 at six urban areas by GPS observations. The result shows less RMSE of about 1.9 m and large disagreement at position and features. Consequently, we suggests that l/5,000 digital map is necessary of whole revision.