• Title/Summary/Keyword: 1,4-첨가

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Fermentation Properties of Yogurt Added by Lycii fructus, Lycii folium and Lycii cortex (구기자, 구기엽 및 지골피를 첨가한 요구르트의 발효 특성)

  • 조임식;배형철;남명수
    • Food Science of Animal Resources
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    • v.23 no.3
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    • pp.250-261
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    • 2003
  • This experiment was carried out to examine the fermentation properties of yogurt with Lycii fructus, Lycii folium and Lycii cortex powder, and extract additives at concentrations of 0.5, 1.0, 2.0, 4.0, and 6.0%. Lactic acid bacteria was used in a mixed starter culture of Streptococcus salivarius ssp. thermophilus(ST36) and Lactobacillus delbrueckii ssp. bulgaricus(LB12). When the boxthorn was added with extract types, the changes of pH, acidity and lactic acid bacteria counts of yogurt during the fermenation of 3 hours were pH 5.64, titratable acidity 0.85%, 5.80xl0$\^$6/cfu/ml of viable cell counts for control yogurt, whereas those were pH 4.10∼5.06, titratable acidity 0.98∼1.27%, 1.80∼9.60x10$\^$7/ cfu/ml of viable cell counts for Lycii fructus extract yogurt. The lactose hydrolysis ratio was better for 1.0% Lycii fructus extract yogurt(42.00%) and 1.0% Lycii folium extract yogurt(41.46%) than for control yogurt(28.40%). Also, content of lactic acid of 1.0% Lycii fructus(11.9 times) and 1.0% Lycii folium extract yogurt(10.6 times) produced more than control yogurt(7.3 times). The viscosity of yogurt was better for boxthorn extract yogurt(1,027∼1,382 cps) than for control yogurt(975cps). The sensory scores of color, taste and overall acceptability of yogurt with 0.5, and 1.0% Lycii fructus extract additive were better than other groups. The yogurts made with increased Lycii fructus extract concentration(0.5∼6.0%), showed the increase of lactic acid, titratable acidity, number of lactic acid bacteria, viscosity and lactose hydrolysis rate compared to the treatments of 0.5, 1.0, 2.0, and 4.0% Lycii folium and Lycii cortex extract and powder yogurt. We gained excellent results from the yogurt to which Lycii fructus extract was added with 0.51.0% concentration.

Enhanced Expression of Foreign Gene in Baculovirus-Infected Insect Cells Using a Silkworm Hemolymph (누에 체액을 이용한 외래 유전자의 발현효율 증대)

  • 우수동;김혜성
    • Journal of Sericultural and Entomological Science
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    • v.37 no.2
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    • pp.181-185
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    • 1995
  • To enhance expression of foreign gene by the novel expression vector, pBmKSK1, of Bombyx mori nuclear polyhedrosis virus, E. coli $\beta$-galactosidase gene expressing recombinant virus was infected in BmN-4 cells and various concentrations of silkworm hemolymph were added to the recombinant virus-infected BmN-4 cells containing fetal bovine serum. The expression efficiency of foreign gene was determined by $\beta$-galactosidase activity in the culture media. The results showed that the silkworm hemolymph was effective to expression of foreign gene in the BmN-4 cells, suggesting that the silkworm hemolymph could be substituted for fetal bovine serum in the BmN-4 cells to enhance expression of foreign gene.

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Quality Characteristics of Lamb Stock According to Salt Contents - Using a High Pressure Extraction Cooking Method - (소금첨가량을 달리한 양 육수의 품질 특성 - 고압가열추출방식으로 -)

  • Hong, Woo-Pyo;Kim, Dong-Seok;Choi, Soo-Keun
    • Culinary science and hospitality research
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    • v.18 no.2
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    • pp.149-161
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    • 2012
  • The purpose of this study is to examine the quality characteristics of lamb stock by different salt contents in it. For this purpose, mechanical tests (color value, pH, sugar content, salinity, amino-acid content) and sensory evaluation (attribute difference test & acceptance) were conducted, showing the following results; Salinity and sugar increased significantly (p<0.01, p<0.001) with salt contents increased. The 0.1% salt addition group contained 32 kinds of free amino-acids, while 0.2% and 0.3% contained 34. 0.4% salt addition group turned out to contain 34 kinds of free amino-acid in it. The amount of the free amino-acid content was also the highest with 0.3% sample group, followed by 0.2%, 0.4%, and 0.1% sample group. In the test group, 9 kinds of essential amino-acid were discovered, and all samples contained histidine and arginine, which were essential in the growth of children. Among all samples, arginine appeared to be the highest in content. Each of the essential amino-acid content varied with statistical significance among the sample groups. In addition, 14 kinds of amino-acids other than the above mentioned were discovered, as well. The 0.3% salt addition group showed the highest 'after taste' value, and the differences among the sample groups were statistically significant(p<0.001). The acceptance test of the lamb stock showed that the flavor and mouth feel did not vary among the sample groups with statistical significance. The control group had the highest value for appearance, measuring scoring 4.32, and the sample group of 0.2% salt addition showed the lowest value of appearance, scoring 3.63. As for the preference on the taste, the highest value of 4.09 came from the 0.3% salt addition group. The differences between the sample groups were statistically significant. Finally, with regard to the overall quality, the 0.3% salt addition group had the highest value of 4.23 while the control group scored the lowest, 3.52. The differences among the samples were statistically significant. The overall result of the study indicated that the optimal rate of salt addition in making lamb stock was 0.3%.

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Effects of the Addition of Amino Acids and FBS on the In Vitro Development of Korean Native Cow IVP Embryos (아미노산과 FBS의 첨가가 한우난자의 체외발달에 미치는 영향)

  • Park, Heum-Dae;Park, Hyang;Lee, Sang-Jin;Kim, Jae-Myung
    • Journal of Embryo Transfer
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    • v.17 no.3
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    • pp.211-218
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    • 2002
  • These studies were carried out to investigate the effects of the addition of amino acids and FBS as source of exogenous nitrogen fixation added to medium on in vitro production of blastocyst derived from bovine follicular oocytes. The base medium was TCM-199 solution for in vitro maturation(IVM) of bovine follicular oocytes and Fer-TALP solution for in vitro fertilization(IVF) and YS solution for in vitro culture(IVC). IVC used the fertilized oocytes of 24-hr culture (day 1) after IVF. Rmbryos were cultured in drop-culture that contained 25 embryos per 10 ${mu}ell$. The results obtained are as follows: 1 The developmental rates of fertilized oocytes to blastocyst that developed from YS solution with NEAA derived from MEM alone were higher than those of YS solution without NEAA. 2. The developmental rates of fertilized oocytes to blastocyst that developed from YS solution with EAA derived RPMI 1640 alone were significantly higher than those of YS solution without EAA (p<0.05). 3. When added to EAA on day 5 after NEAA supplementation on day 1, the developmental rates of hatched blastocyst and blastocyst to hatched blastocyst were improved. 4. When removed to EAA on day 3, day 4 and day 5 from medium after NEAA and EAA supplementation on day 1. the developmental rates of blastocyst to hatched blastocyst were reduced. 5. When added to FBS as source of exogenous nitrogen fixation, the developmental rates of blastocyst and hatched blastocyst that developed from the later culture higher(day 5) than those of the early culture.

Comparison of Biological Activity Between Soybean Pastes Adding Sword Bean and General Soybean Pastes (작두콩 첨가 된장과 일반 된장의 생리활성 특성 비교)

  • Lee, Hak-Tae;Kim, Jong-Ho;Lee, Sang-Sun
    • Journal of Food Hygiene and Safety
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    • v.24 no.1
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    • pp.94-101
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    • 2009
  • This study was carried out to analyse the biological activity in soybean pastes with the sword beans and general soybean pastes. The electron donating activity of soybean pastes with the sword beans(J2, J4) was higher than other soybean pastes with value of $81.5{\pm}2.2%$ and $88.3{\pm}0.7%$, respectively. And the content of total phenol compounds was higher in J2 and J4 compared to the others with value of $1773.8{\pm}2.9\;mg/kg$ and $1785.7{\pm}3.9\;mg/kg$ respectively. ACE inhibitory effect was higher in J2 and J4 than other soybean pastes with value of $63.1{\pm}1.0%$, $62.1{\pm}1.4%$, respectively. And aglycone type(daidzein, genistein) isoflavone content was higher than glucoside type(daidzin, genistin). But tyrosinase inhibitory effect was not different among test samples. From the results, soybean pastes with the sword beans(J2, J4) have higher anti-oxidant activity and ACE inhibitory effect than other soybean pastes, therefore, it may have potential to prevent hypertension.

Properties of the Mixed Fermentation Milk Added with Red Ginseng Extract (홍삼 추출물 첨가 혼합 발효유의 특성)

  • Bae Hyoung-Churl;Nam Myoung-Soo
    • Food Science of Animal Resources
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    • v.26 no.1
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    • pp.127-135
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    • 2006
  • This experiment was carried out to examine the fermentation properties of yogurt with bovine milk and soybean milk at the mixed ratio of 2:1 and added 0.1, 0.2, 0.4 and 1.0% red ginseng extract. The effect on promoting the fermentation by additives 0.1, 0.2, 0.4 and 1.0% red ginseng extracts were higher and pH was $3.90{\sim}3.94$ when Lactobacillus acidophilus KCTC3150 and Lactobacillus salivarius ssp. salivarius CNU27 were used. Titratable acidity showed a little inhibiting due to increasing red ginseng extract content. The average viable counts of lactic acid bacteria after 15 hour culture was the highest level of $6.26{\times}10^8cfu/mL$ when Lactobacillus acidophilus KCTC3150 was used, and the additives content of red ginseng extract was 1.0% The production of lactic acid was the highest and the concentration was 332.22 mM when Lactobacillus acidophilus KCTC3150 was used, and the additives content of red ginseng extracts was 1.0% Lactose hydrolysis was completely hydrolyzed when Lactobacillus acidophilus KCTC3150 and Lactobacillus salivarius ssp. salivarius CNU27 were used. The highest viscosity of yogurt was 780 cP when Lactobacillus acidophilus KCTC3150 and Lactobacillus salivarius subsp. salivarius CNU27 were used and red ginseng extract was added 1.0% The overall acceptability, $4.17{\pm}0.64$, was the highest when Lactobacillus salivarius subsp. salivarius CNU27 was used and the additives content of red ginseng extract was 0.2%.

The Effect of Roasted Soybean Flour Supplimentation to Jeolpyons(Korean Rice Cake) on Changing the Contents of Amino Acid, Amylose, and Minerals (볶은 콩가루 첨가가 절편의 아미노산, 아밀롯 및 무기물 함량에 미치는 영향)

  • 정해옥;정복미
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.4
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    • pp.614-617
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    • 1994
  • This study was undertaken to determine the changes in amino acids, amylose and mineral contents of Jeolpyons(Korean rice cake) supplemented with various levels of roasted soybean flour (RSF). Amino acids content of Jeolpyons increased as RSF content increased. Especially the contents of total essential amino acids of Jeolpyon supplemented with RSF(5, 10, 15 and 20%) increased 1.22 to 2.74 times greater than those of the ocntrol. Amylose, which is related to retrogradatin effect on rice products decreased about 0.9 to 4.7% by increasing the soybean flour contents. Mineral contents by jeolpyon containing RSF increased as RSF contents increased.

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Effects of Dietary Kaolin on the Performance, Product Quality and Feeding Environment of Growing Ducks (고령토를 첨가한 사료의 급여가 육용오리의 능력과 생산물의 품질 및 사육환경에 미치는 영향)

  • Lee W. J.;Lee K. H.
    • Korean Journal of Poultry Science
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    • v.32 no.2
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    • pp.81-87
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    • 2005
  • A study was carried out to examine the effects of dietary kaolin on the performance, feed and nutrient efficiency, meat quality and feeding environment using 200 day-old growing ducts. Four replicates of 10 birds each was assigned to diets containing 0(C), 1 (T1), 2(T2), 3(T3) and $4(T4)\%$ of kaolin. Body weight gain in T1 and T2 were higher than in $C(p<0.05)$ whereas weight gain in T3 and T4 were not significantly different from the control. Feed intake and feed conversion ratio tended to increased as the level of dietary kaolin increased, but without significant differences among treatments. Mortality of growing ducks during 6 week period was not significantly different among treatments. Dressing percentage was highest in T1 and lowest in T4 $(p<0.05)$. The utilizability of nutrients except for crude fat Increased$(p<0.05)$, as the level of dietary kaolin increased. Moisture content and $NH_3$ gas emission of excreta was not significantly influenced by dietary kaolin. $H_2S$ gas emission of excreta in T2, T3 and T4 was lower compare to that in C and $T1(p<0.05)$. Cholesterol content in breast and thigh meat was not different among treatments. Values for pH, WHC, TBARS, POV and Cooking loss of breast and thigh meat were not significantly different or did not show any trend among treatments.

Quality Characteristics of White Pan Bread with Mesangi(Capsosiphon fulvecense) (매생이를 첨가한 식빵의 품질 특성)

  • An, Hye-Lyung;Lee, Kwang-Suck;Park, Sang-Jun
    • Journal of the East Asian Society of Dietary Life
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    • v.18 no.4
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    • pp.563-568
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    • 2008
  • The principal objective of this study was to determine the optimal ratio of mesangi added to bread. In our experiments, the mesangi was added at levels of 0, 1, 2, 3, 4%. The properties of the prepared breads were analyzed in terms of volume of dough, specific volume, CrumbScan, color and sensory evaluation. The volume of the dough to which 1% mesangi has been added was the greatest and the specific volume of the bread to which 2% mesangi was added was also greater than that of the control bread. The bread to which 4% mesangi has been added evidenced the highest elongation at 1.39, the most profound thickness(0.05 cm) and the most prominent chromaticity of the skin. With regard to color values, as more mesangi was added the L value decreased. As the result of the sensory evaluation, the bread to which 1% mesangi was added was found to be the most preferable in terms of moistness, softness, taste and overall acceptability.

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Thermoelectric Properties of Al4C3-doped α-SiC (Al4C3 첨가 α-SiC의 열전변환특성)

  • 박영석;배철훈
    • Journal of the Korean Ceramic Society
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    • v.40 no.10
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    • pp.991-997
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    • 2003
  • The effect of A1$_4$C$_3$ additive on the thermoelectric properties of SiC ceramics were studied. Porous SiC ceramics with 47∼59% relative density were fabricated by sintering the pressed $\alpha$-SiC powder compacts with A1$_4$C$_3$at 2100∼220$0^{\circ}C$ for 3 h in Ar atmosphere. Crystalline phases of the sintered bodies were identified by powder X-Ray Diffraction (XRD) and their microstructures were observed with a Scanning Electron Microscope (SEM). In the case of A1$_4$C$_3$ addition, the phase transformation of 6H-SiC to 4H-SiC could be observed during sintering. The Seebeck coefficient and electrical conductivity were measured at 550∼95$0^{\circ}C$ in Ar atmosphere. In the case of undoped specimens, the Seebeck coefficients were positive (p-type semiconducting) possibly due to a dominant effect of the acceptor impurities (Al, Fe) contained in the starting powder and electrical conductivity increased as increasing sintering temperature. Electrical conductivity of A1$_4$C$_3$doped specimen is larger than that of undoped specimen under the same condition, which might be due to the reverse phase transformation and increasing of carrier density. And the Seebeck coefficient of A1$_4$C$_3$ doped specimen is also larger than that of undoped specimen. The density of specimen, the amount of addition and sintering atmosphere had significant effects on the thermoelectric property.