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Effects of the Addition of Amino Acids and FBS on the In Vitro Development of Korean Native Cow IVP Embryos  

Park, Heum-Dae (Department of Biotechnology, Daeju University)
Park, Hyang (Department of Biotechnology, Daeju University)
Lee, Sang-Jin (Department of Animal Science, Sahm Yook University)
Kim, Jae-Myung (Infertility Medical Center of CHA General Hospital, Pochon CHA University)
Publication Information
Journal of Embryo Transfer / v.17, no.3, 2002 , pp. 211-218 More about this Journal
Abstract
These studies were carried out to investigate the effects of the addition of amino acids and FBS as source of exogenous nitrogen fixation added to medium on in vitro production of blastocyst derived from bovine follicular oocytes. The base medium was TCM-199 solution for in vitro maturation(IVM) of bovine follicular oocytes and Fer-TALP solution for in vitro fertilization(IVF) and YS solution for in vitro culture(IVC). IVC used the fertilized oocytes of 24-hr culture (day 1) after IVF. Rmbryos were cultured in drop-culture that contained 25 embryos per 10 ${mu}ell$. The results obtained are as follows: 1 The developmental rates of fertilized oocytes to blastocyst that developed from YS solution with NEAA derived from MEM alone were higher than those of YS solution without NEAA. 2. The developmental rates of fertilized oocytes to blastocyst that developed from YS solution with EAA derived RPMI 1640 alone were significantly higher than those of YS solution without EAA (p<0.05). 3. When added to EAA on day 5 after NEAA supplementation on day 1, the developmental rates of hatched blastocyst and blastocyst to hatched blastocyst were improved. 4. When removed to EAA on day 3, day 4 and day 5 from medium after NEAA and EAA supplementation on day 1. the developmental rates of blastocyst to hatched blastocyst were reduced. 5. When added to FBS as source of exogenous nitrogen fixation, the developmental rates of blastocyst and hatched blastocyst that developed from the later culture higher(day 5) than those of the early culture.
Keywords
essential amino acid; nonessential amino acid; FBS; embryo development;
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