• Title/Summary/Keyword: 01A29

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Molecular Cloning, mRNA Expression, and Localization of the G-protein Subunit Galphaq in Sheep Testis and Epididymis

  • Li, Zhen;Lu, Jieli;Sun, Xiaowei;Pang, Quanhai;Zhao, Yiwen
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.12
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    • pp.1702-1709
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    • 2016
  • The reproductive function of G-protein subunit Galphaq (GNAQ), a member of the G protein alpha subunit family, has been extensively studied in humans and rats. However, no data is available on its status in ruminants. The objectives of this study were to evaluate the expression pattern of the GNAQ in the testis and epididymis of sheep by polymerase chain reaction (PCR). The mRNA expression levels were detected by real-time fluorescent quantitative PCR, and cellular localization of GNAQ in the testis and epididymis was examined by immunohistochemistry. Additionally, GNAQ protein was qualitatively evaluated via western blot, with the results indicating that similarities between GNAQ mRNA levels from sheep was highly conserved with those observed in Bos taurus and Sus scrofa. Our results also indicated that GNAQ exists in the caput and cauda epididymis of sheep, while GNAQ in the testis and epididymis was localized to Leydig cells, spermatogonial stem cells, spermatocytes, Sertoli cells, spermatid, principal cells, and epididymis interstitial cells. The concentrations of GNAQ mRNA and protein in the caput and cauda epididymis were significantly greater than those observed in the corpus epididymis (p<0.01) and testis (p<0.05). Our results indicated that GNAQ exists at high concentrations in the caput and cauda epididymis of sheep, suggesting that GNAQ may play an important role in gonad development and sperm maturation.

The Study of Speech Rate in Normal-Speaking Adults and Children (정상 성인 및 아동의 구어속도에 관한 연구)

  • Ahn, Jong-Bok;Shin, Myung-Sun;Kwon, Do-Ha
    • Speech Sciences
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    • v.9 no.4
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    • pp.93-103
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    • 2002
  • The purpose of this study was to establish preliminary data on the speech rates in groups of normal speaking adults and children. The results of the present study are intended to serve as clinical measurement guidelines for diagnosis, assessment, treatment planning, and therapy progresses of stuttering. Thirty-one adults (16 females, 15 males), aged 18-30 years and thirty normally developing children (15 females, 15 males), aged 8-10, participated in the study. The subjects' reading of the Stroll (Jeong, 1994) passage and l-minute portion of talking about the daily routine were sampled. The adult speakers had rates of $308.29\pm22.57$ syllables per minute (SPM) or $108.06\pm6.17$ words per minute (WPM) during reading, and $252.87\pm40.86$ SPM and $92.26\pm17.12$ WPM during talking. The children had rates of $176.67\pm33.65$ SPM or $64.07\pm12.62$ WPM during reading, and $149.30\pm33.14$ SPM and $56.60\pm11.36$ WPM during talking. The results of t-tests for reading and talking tasks in adults showed that SPM in reading (t=2.211, p< .05) and WPM in talking (t=-2.284, p< .05) differed significantly by the gender. To answer the questions whether the rate is different across children' s gender and age, a two-way ANOVA was performed. Both SPM and WPM in reading tasks were significantly different between groups of children aged 8 and 10 (p< 01), In speaking tasks, both SPM and WPM were significantly different between groups of children aged 8 and 10, and between 9 and 10.

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Factors influencing awareness of dental health insurance among adults (성인의 치과건강보험 인식과 영향 요인 연구)

  • Lee, Kyeong-Hee;Lee, Sae-Bom;Jung, Eun-Young;Jo, Eun-Byeol;Jung, Eun-Seo
    • Journal of Korean society of Dental Hygiene
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    • v.18 no.5
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    • pp.771-783
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    • 2018
  • Objectives: This study aimed to identify methods that help medical consumers to be aware of relevant information regarding changes in dental health insurance policy. Methods: The following results were obtained from a survey regarding awareness and satisfaction with dental health insurance policy among the general public aged 20 years or older in Seoul, Gyeonggi Province, and other areas from August 1, 2017 to August 23, 2017. Results: The correlation between adults' awareness of and satisfaction with dental health insurance was 0.471, where satisfaction with dental health insurance was enhanced with increasing awareness of dental health insurance (p<0.001). Factors that significantly affected awareness of dental health insurance were marriage (married p<0.05), medical service (dental university hospital, p<0.01), and satisfaction with dental health insurance (p<0.001); these factors explained 29.2% of the variance. Factors that significantly affected satisfaction with dental health insurance were final education (high school graduation, p<0.05), medical service (dental hospital, p<0.05), and awareness of dental health insurance (p<0.001); these factors explained 27.8% of the variance. Conclusions: Importantly, awareness of the government's operation of dental health insurance and the benefits of such dental health insurance were low, suggesting that the government and national health insurance agency should improve the level of transparency in dental health insurance operations and continuously promote dental health insurance benefits to the public.

Determination of Energy and Time Requirement for Cooking Pigeon Pea (Cajanus cajan)

  • Akinoso, Rahman;Oladeji, Ojeronke Dewum
    • Journal of Biosystems Engineering
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    • v.42 no.1
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    • pp.56-61
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    • 2017
  • Purpose: High energy requirement and long cooking time are limiting consumption of pigeon pea (Cajanus cajan), a nutritious food. This study was performed to estimate energy and time demand by different methods of cooking pigeon pea. Methods: Pigeon pea (150 g) was soaked in 2.0 L of water at ambient temperature ($29{\pm} 2^{\circ}C$) to determine hydration behavior. Cooking experiments were conducted using aluminum and pressure-cooking pots. Efficiency of cooking was evaluated using four types of cooking appliances (kerosene, liquefied petroleum gas (LPG), electric, and charcoal stoves). Normal (continuous heating until the food was satisfactorily cooked) and control (controlling the energy input to closely match the actual energy required) cooking were conducted. Energy requirement and duration of cooking were determined using standard procedures. Results: Soaking increased moisture content from 11.99 to 30.01% in 90 min, while water absorption rate decreased with soaking duration. In cooking 150 g of pigeon pea using kerosene stove, presoaked normal pressure-pot cooking method consumed the least energy (10 800 kJ) and time (205 min), while unsoaked normal cooking consumed the highest energy (18 450 kJ) and time (336 min). Using LPG stove, unsoaked normal cooking method required the highest energy (52 470 kJ), while presoaked control pressure-pot required the least energy (14 405 kJ). For electric stove, the lowest energy (15 560 kJ) and shortest duration (105 min) were recorded during control cooking of presoaked sample in the pressure-pot. Conclusions: Control cooking was not practicable using charcoal stove. Generally, kerosene stove consumed the least energy, while electric stove was found to have the shortest duration of cooking.

A Survey on Elementary, Middle and High School Students' Attitude and Eating Behaviors about Kimchi in Seoul and Kyunggido Area (서울.경기지역 초.중.고등학생의 김치 섭취 실태 및 태도 조사)

  • Moon, Hae-Jin;Lee, Young-Mee
    • Journal of the Korean Society of Food Culture
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    • v.14 no.1
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    • pp.29-42
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    • 1999
  • This study was performed with 482 subjects(278 boys and 204 girls in Seoul and Kyunggido area) to find out the attitude and eating behaviors about Kimchi on Korean young generation. General characteristics about subject and attitude and eating behavior were surveyed by self-developed questionnaires. 85.9% of the subjects were nuclear family type and 49.9% of respondent's mother had full-time job and 96.1% of subjects were middle or upper economic status. The preference for Kimchi, 60.4% of respondents answered that they liked Kimchi. According to grade, the rate of preference about Kimchi was significantly different, higher grade students were found higher preference on Kimchi : 64.4% of middle and 70.4% of high school students liked Kimchi. The eating patterns of Kimchi, up to 60% of respondents answered they ate Kimchi in every meal time, which suggested that it was still an important side dish in Korean meal among teenagers. The eating behaviors on Kimchi, $85{\sim}90%$ of respondents answered that they preferred Kimchi in meal time. Baechu Kimchi(chinese cabbage Kimchi) and Gakdugi(radish cube Kimchi) was highly preferred. And their preference of food which made from Kimchi (such as Kimchi fried rice, Kimchi stew, Kimchi pancake) was highly evaluated. 65.6% of residents pointed out that 'Kimchi is very nutritious food'. The respondent's structure of value on Kimchi were not significantly difference between sex and age. But it was significantly difference(p<0.01) between the groups with preference and familiarity on Kimchi. The group had higher preference and familiarity on Kimchi agreed to the positive values about Kimchi. These result suggested that various kinds of Kimchi should be developed for young generation to meet their taste and also continuous nutrition education about traditional food especially Kimchi should be promoted at home and school.

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Clinical Effect of Acupuncture Combined with Herbal Medicine in Treatment of PSD: A Systematic Review and Meta-Analysis (뇌졸중 후 우울증에 대한 침과 한약의 결합 치료 효과: 체계적 문헌 고찰 및 메타분석 연구)

  • Sakong, Jong-Won;Lim, Su-Yeon;Kim, Sang-Ho;Chung, Dae-kyoo
    • Journal of Oriental Neuropsychiatry
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    • v.31 no.3
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    • pp.169-186
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    • 2020
  • Objectives: The purpose of this study was to provide clinical evidence to support the use of acupuncture combined herbal medicine for post-stroke depression. Methods: Studies were identified by searching Pubmed, EMBASE, Cochrane Library, NDSL, RISS, KMbase, KISS, and CNKI. Randomized controlled trials that verified effects of the acupuncture combined with the herbal medicine for PSD were included in this study. Literature searches of English, Chinese, and Korean databases were performed. Two authors independently extracted the data and assembled the study quality. Results: Of 698 RCTs screened, 17 RCTs were selected. Most of the studies had unclear risk of bias. Among the 17 RCTs, 10 were meta-analyzed. As for the outcome measurement, the effective rate and the HAMD were most commonly used. The meta-analysis of the studies revealed that the PSD cure effective rate in the acupuncture combined with herbal medicine group was higher than that in the Western medicine group (RR: 1.23, 95% CI: 1.14 to 1.33, p<0.00001, I2=0%). Also, the HAMD in the acupuncture combined with herbal medicine group was significantly different compared to the Western medicine group (MD -5.54, 95% CI -9.80 to -1.29, p=0.01, I2=99%). The quality of the selected RCTs was low. Conclusions: Acupuncture combined with herbal medicine is effective for treating post-stroke depression. However, It is worth noting that the included studies were of relatively poor quality. The sample sizes were also small. Based on the findings of this study, further investigations into the diagnosis and treatment of PSD are warranted.

Effect of Seeding Rate on Growth, Yield and Chemical Composition of Forage Rape Cultivars

  • Cho, Nam-Ki;Jin, Woo-Jong;Kang, Young-Kil;Kang, Bong-Kyoon;Park, Yang-Mun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.1
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    • pp.54-58
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    • 1998
  • Four introduced forage rape cultivars 'Akela', 'Ramon', 'Sparta', and 'Velox' and a leading rapeseed cultivar Hall-ayuchae were seeded on 1 October 1994 on 20-cm rows at seeding rates of 3, 5, 7, 10, 13, and IS kg/ha to select the best adapted forage rape cultivars and to determine the optimum seeding rate in the Cheju area. Days to flowering of each cultivar was not significantly affected by seeding rate. Average plant height increased from 151 to 164 cm as seeding rate increased from 3 to 10 kg/ha and then decreased to 156 cm at 15 kg/ha. Stem diameter linearly decreased with increasing seeding rate. The optimum seeding rate for the greatest dry matter and protein yields of five cultivars ranged from 10.7 to 11.8 and 10.8 to 14.4 kg/ha, respectively. Dry matter yields of five cultivars ranged from 7.72 and 22.01 Mg/ha. Sparta produced the greatest dry matter yield, followed by Akela, Ramon, Hallayuchae, and Velox. Crude protein yields of five cultivars ranged from 0.29 to 1.08 Mg/ha. Sparta produced the greatest crude protein yield, followed by Akela, Ramon, Velox, and Hallayuchae. As seeding rate increased, crude protein content linearly increased but crude fiber content linearly decreased. The forage cultivars had higher crude protein and lower crude fiber than the oilseed cultivar Hallayuchae. Our results demonstrate that Sparta was the best adapted cultivar to Cheju area and the optimum seeding rate for Sparta was about 10 kg/ha.

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Effects of Saururus chinensis Baill on Glutathione and Antioxidative Activity Against TCDD-treated Rats (환경 Hormone에 대한 삼백초의 Glutathione 및 항산화 활성 효과)

  • Ha, Bae-Jin
    • Journal of Food Hygiene and Safety
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    • v.18 no.3
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    • pp.161-165
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    • 2003
  • The effects of Saururus chinensis Baill administration on the biochemical parameters of function in liver of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treated rats were investigated. Saururus chinensis Baill (200mg/kg) was administered into rats intraperitoneally for four weeks, seven days after the injection of TCDD(1 ${\mu}g/kg$). We examined the antioxidative enzymatic activity by measuring the level of AST and ALT in serum and SOD, Catalase, GPx, GSH and GSSG in liver tissue of rats. STT group showed 70.7% of inhibitive effect in AST acrivity compared to TTA group. ALT level of STT group was decreased to the level of NTT group. SOD and Catalase in TTA group were lower than in NTT group, but SOD and Catalase in STT group were increased by 82% and 55.45% respectively compared to TTA group. GSH contents in STT group were 74.20% increased compared to TTA group. GSSG contents in STT group were 61.08% decreased compared to TTA group. These results suggest that Saururus Chinensis Baill has a potent hepathprotective effect against TCDD intoxicated rats.

Optimization of the Manufacturing of Process Butter by Response Surface Methodology and Its Texture and Rheological Properties (반응표면분석법에 의한 가공버터 제조의 최적화 및 Rheology 분석)

  • Suh, Mun-Hui;Yoon, Kyeong;Baick, Seung-Chun
    • Journal of Dairy Science and Biotechnology
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    • v.26 no.2
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    • pp.51-56
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    • 2008
  • Using central composite design, we have designed optimization of the manufacturing of processed butter. And response surface analysis by least-square regression was used Statistical Analysis System(SAS). Central composite design can be achieved by response surface techniques that allow flexibility in modeling and analysis. Response surface methodology(RSM) was used to optimize hardness(%) using as independent variables; the content of butter($X_1$), ranging from 50 to 90(%), the content of soybean oil($X_2$), from 0 to 20(%), and the hydrogenated soybean oil($X_3$) from 0 to 4(%). The results on the regression coefficients calculated for overrun by response surface by least-square regression(RSREG) were followed. It was considered that the linear regression was significant(p<0.01). As for the processed butter, the regression model equation for the hardness(Y, %) to the change of an independent variable could be predicted as follow: $Y=60.88-8.92X_2-{29.3X_2}^2$. The optimal for the manufacturing of processed butter were determined at the content of butter of 88.22%, soybean oil of 6.71% and hydrogenated soybean oil of 2.36%, respectively. Optimum compositions were resulted in hardness of 65.78 N. Finally the reference sample(Butter in the morning, Seoul Dairy Co-op.) and processed butter manufacturing under the optimal conditions were compared with spreadability test. The spreadability scores result from reference sample and butter under optimal conditions was not found a significant difference.

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Study on Prognostic Factors using Computerized Tomographic Findings and Ability in daily Life(ADL) Evaluation in Patients with Hypertensive Intracerebral Hemorrhage (고혈압성(高血壓性) 뇌출혈(腦出血) 환자(患者)의 Brain-CT소견과 일상생활능력(ADL)평가를 통한 예후인자에 대한 고찰)

  • Jung, Seung-Hyun;Shin, Gil-Cho;Lee, Won-Chul
    • The Journal of Korean Medicine
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    • v.18 no.1
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    • pp.87-100
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    • 1997
  • Clinical Observation was made on 29 cases of Hypertensive intracerebral hemorrhage patients in the ICU of In-Chon Oriental Medical Hosptital of Dongguk University from October in 1994 to June in 1996. The observation are ability in daily life(ADL) of patients by Location and Type of Hemorrhage, Amounts of Hematoma, Graeb's Score, Intraventricular Hemorrhage, States 4th Ventricle, Surrounding Edema around the Hematoma, Middle Line Shift, Age, Level of Consciousness. Pupillary Light Reflex and Treatment Modalities. Our conclusions on Prognostic Factors using Computerized Tomographic Findings and Ability in daily Life(ADL) Evaluation in patients with Hypertensive Intracerebral Hemorrhage Patients are as follows. A variety of prognostic factors that influence ADL5+6(%) were observed. 1. ADL5+6($\%$) of total cases was 34.9%. The prognosis were unfavorable when high Graeb score(P<0.05), dilated 4th ventricle(P<0.01), much surrounding edema around the hematoma (P<0.05), unilateral unreactive or both unreactive pupillary light reflex(P<0.05). 2. There was no difference of ADL5+6(%) in both hypertensive basal ganglionic and thalamic intracerebral hemorrhage. 3. The prognosis gets poorer as the volume of hematoma is more than 16cc. But there was no difference of ADL5+6(%) in each group. 4. The prognosis gets poorer in cases with IVH than without IVH. But there was no difference of ADL5+6(%) in each group. 5. The prognosis gets poorer as the middle line shift is more than 6mm. But there was no. difference of ADL5+6(%) in each group. 6. The prognosis gets poorer as the level of consciousness is more than drowsy. But there' was no difference of ADL5+6(%) in each group.

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