• Tuberculosis and Respiratory Diseases
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• 제61권3호
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• pp.285-288
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• 2006

인터페론 알파는 만성활동성C형간염 환자에서 항바이러스제로 사용되는 면역조절제로, 인터페론 치료가 사르코이드증을 발생시키거나 악화시킬 수 있다고 알려져 있다. 저자 등은 C형간염바이러스 활성화로 인터페론과 ribavirin으로 치료를 받은 후 기존의 폐사르코이드증이 악화되었다가 자연 호전된 예를 경험하였기에 보고하는 바이다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권13호
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• pp.5515-5519
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• 2015

The single nucleotide polymorphism (SNP) ss469415590 in the interferon lambda-4 (IFNL4) gene has recently been reported to have an association with treatment response in chronic hepatitis C. However, any importance of the SNP in association with response to pegylated interferon (PEG-IFN) therapy in patients with chronic hepatitis B (CHB) is unclear. We retrospectively analyzed data for Thai patients with CHB treated with PEG-IFN for 48 weeks. Virological response (VR) for HBeAg-positive CHB was defined as HBeAg seroconversion plus HBV DNA level <2,000 IU/mL at 24 weeks post-treatment. VR for HBeAg-negative CHB was defined as an HBV DNA level <2,000 IU/mL at 48 weeks. The SNP was identified by real time PCR using the TaqMan genotyping assay with MGB probes. A total 254 patients (107 HBeAg-positive and 147 HBeAg-negative) were enrolled in the study. The distribution of TT/TT, ${\Delta}G/TT$ and ${\Delta}G/{\Delta}G$ genotypes was 221 (87.0%), 32 (12.6%) and 1 (0.4%), respectively. Patients with non-TT/TT genotypes had significantly higher baseline HBV DNA levels than patients with the TT/TT genotype. In HBeAg-positive CHB, 41.2% of patients with TT/TT genotype versus 50.0% with non-TT/TT genotype achieved VR (P=0.593). In HBeAg-negative CHB, the corresponding figures were 40.3% and 43.5%, respectively (P=0.777). There was no significant correlation between the SNP genotypes and HBsAg clearance in both groups of patients. In summary, ss469415590 genotypes were not associated with response to PEG-IFN in Thai patients with HBeAg-positive and HBeAg-negative CHB.

• Fisheries and Aquatic Sciences
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• 제21권3호
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• pp.5.1-5.8
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• 2018

Stimulator of interferon gene (STING) is induced by various inflammatory agents, such as lipopolysaccharide and microbial pathogens, including virus and bacteria. In this study, we obtained a full-length cDNA of a STING homolog from olive flounder using rapid amplification of cDNA ends PCR technique. The full-length cDNA of Paralichthys olivaceus STING (PoSTING) was 1442 bp in length and contained a 1209-bp open reading frame that translated into 402 amino acids. The theoretical molecular mass of the predicted protein sequence was 45.09 kDa. In the PoSTING protein, three transmembrane domains and the STING superfamily domain were identified as characteristic features. Quantitative real-time PCR revealed that PoSTING expressed in all the tissues analyzed, but showed the highest level in the spleen. Temporal expression analysis examined the significantly upregulated expression of PoSTING mRNA after viral hemorrhagic septicemia virus (VHSV) stimulation. In contrast, no significant changes in the PoSTING expression were detected in Edwardsiella tarda-challenged group compared to the un-injected control. The expression of P. olivaceus type I interferon (PoIFN-I) was also highly upregulated upon VHSV challenge. These results suggest that STING might be involved in the essential immune defense against viral infection together with the activation of IFN-I in olive flounder.

• Tuberculosis and Respiratory Diseases
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• 제70권1호
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• pp.69-73
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• 2011

The combination therapy of pegylated interferon and ribavirin is the mainstay of treatment for chronic hepatitis C patients. Anti-viral therapy is commonly associated with side effects such as headache, fever, myalgia, and arthralgia. However, anti-viral therapy can continue because these side effects are mostly mild and can be improved with supportive management. Anti-viral therapy should be stopped promptly if serious side effects, such as interstitial pneumonitis or hemolytic anemia occur, although those serious side effects are rare. There were a few case reports of interferon-related interstitial pneumonitis worldwide. In Korea, one atypical case report of interstitial pneumonitis has been reported, which followed the combination therapy of interferon-alpha and ribavirin in a patient with chronic hepatitis C. We present a case of interstitial pneumonitis and pancytopenia following the combination therapy of pegylated interferon and ribavirin in a patient with chronic hepatitis C.

• 한국미생물·생명공학회지
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• 제46권4호
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• pp.320-325
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• 2018

E형 간염바이러스는 전세계적으로 매년 2천만건의 감염을 일으키는 것으로 알려져 있다. 흥미로운 것은 임산부에서 특히 높은 치사율을 보이는데, 무려 20-30%의 사망률을 보인다는 점이다. 면역능이 있는 감염환자에서는 E형 간염바이러스 감염은 별다른 증상 없이 자연 치유되지만, 면역능이 낮은 에이즈환자나 고령환자에서는 만성감염을 일으킬 수도 있으며 사망에 이르게 할 수도 있다. 따라서, E형 간염바이러스의 증식억제에 면역반응의 중요성이 높으며, 특히 인터페론 반응이 중요한 역할을 할 것으로 사료된다. 본 연구팀은 E형 간염바이러스의 프로티아제가 RIG-I 의존적 제 1형 인터페론 반응을 유의미하게 억제하는 것을 관찰하였다. 또한 바이러스 프로티아제가 RIG-I의 하위 신혼전달 분자의 하나인 MAVS의 활성도 농도 의존적으로 억제하는 것으로 분석하였다. E형 간염바이러스에 의한 제 1형 인터페론 반응 억제의 구체적인 작용 기작을 밝힌다면 효과적인 항바이러스제제의 개발의 초석을 놓을 수 있을 것으로 기대된다.

• Biomolecules & Therapeutics
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• 제1권2호
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• pp.266-269
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• 1993

The acute toxicity of a recombinant human interferon $\alpha$A (code name: LBD-007) was evaluated in both sexes of ICR mice, 5 weeks old, by the oral, subcutaneous and intravenous routes of administration. Based on the results, LBD-007 was not considered to induce any toxic effect on the mice in mortalities, clinical findigs, body weights and gross findings. It is suggested that LD$_{50}$ values in mice would be $>48{\times}10^8$ IU/kg in the oral, subcutaneous or intravenous routes.s.

• 생약학회지
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• 제30권4호
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• pp.363-367
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• 1999

Palmultang(PMT) consists of Ginseng Radix Alba, Atractylodis Rhizoma Alba, Hoelen, Glycyrrhizae Radix, Rehmanniae Radix Preparata, Paeoniae Radix, Cnidii Rhizoma and Angelicae Gigantis Radix. PMT enhanced the lucigenin chemiluminescence and the engulfment of fluorescein-conjugated E. coli particles and inhibited the production of nitric oxide in murine peritoneal macrophage. PMT enhanced the production of ${\gamma}-interferon$, interleukin-2 and the cell viability in murine thymocyte, but did not affect the production of interleukin-4. These results indicate that PMT enhances the phagocytosis of macrophage via the stimulation of ${\gamma}-interferon$ production in $T_H1$ cells and the reduction of nitric oxide production in peritoneal macrophage.

• Journal of Microbiology and Biotechnology
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• 제29권12호
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• pp.1873-1881
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• 2019

The innate immune response serves as a first-line-of-defense mechanism for a host against viral infection. Viruses must therefore subvert this anti-viral response in order to establish an efficient life cycle. In line with this fact, Kaposi's sarcoma-associated herpesvirus (KSHV) encodes numerous genes that function as immunomodulatory proteins to antagonize the host immune system. One such mechanism through which KSHV evades the host immunity is by encoding a viral homolog of cellular interferon (IFN) regulatory factors (IRFs), known as vIRFs. Herein, we summarize recent advances in the study of the immunomodulatory strategies of KSHV vIRFs and their effects on KSHV-associated pathogenesis.

• 한국어병학회지
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• 제16권3호
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• pp.139-146
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• 2003

We have investigated the biological effects of recombinant IL-1$\beta$ (rIL-1$\beta$) on the expression of antiviral genes such as Myxovirus-3 (MX-3) and Interferon regulating factor-1 (IRF-1), which are related to type I interferon. When ten micrograms of rIL-1$\beta$ were treated, we observed the stimulatory effect on the expression of these antiviral genes. Interestingly, at the early stage of stimulation, these genes were down-regulated and then up-regulated by the results obtained that the expressions of these genes were decreased at day 1 post-injection and gradually increased at day 3 post-injection. Thus, the stimulatory effect of rIL-1$\beta$ on the expression of MX-3 and IRF-3 gene might be an indirect stimulatory effect because significant up-regulation was delayed until day 3 post-injection.

• KSBB Journal
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• 제5권3호
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• pp.195-200
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• 1990

대장균의 lipoprotein promoter, lactose promotor 및 operator 와 lipoprotein의 signal sequence 를 가지는 vector에alpha-IFN 유전자가 cloning된 plasmid pIF-Ill-B를 여러종류의 대장균 숙주 세포에 형칠전환하여 alpha-IFN 의 생산성, 생장특성을 조사하였다. 또한 plasmid 자체와 cloning된 alpha-IFN 유전자의 발현유도가 세포생장에 미치는 영 향을 조사하였다.