• Title/Summary/Keyword: -cyclodextrin

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Purification and Immobilization of Cyclodextrin glucanotransferase from recombinant Bacillus subtilis

  • Seo, Hyo-Jin;Kim, Yeong-Hwa;Kim, Seong-Gu
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.671-674
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    • 2001
  • Cyclodextrin glucanotransferase(CGTase) derived from recombinant Bacillus subtilis was partial purified and concentrated by ultrafiltration. The prepared CGTase were immobilized on various matrices by ionic interaction or covalent bond. CGTase covalently bound on CNBr-activated sepharose 4B were identified to be the highest immobilization activity among various immobilization methods. The optimum conditions for CGTase immobilization were determined; $30^{\circ}C$, 6Orpm, using O.2g CNBr-activated sepharose 4B in pH 6.0 phosphate buffer and 9hr immobilization.

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Bioavailability Studies on Suspension of Inclusion Complexes of Piroxicam with Cyclodextrins (Piroxicam-Cyclodextrin 포접화합물의 현탁제에 대한 생체내 이용율의 연구)

  • Park, Sun Hee;Lee, Chang Hoon;Choi, Young Wook;Park, Gee Bae;Kim, Johng Kap
    • Korean Journal of Clinical Pharmacy
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    • v.1 no.1
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    • pp.9-14
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    • 1991
  • Inclusion complexes of piroxicam with $\alpha,\;\beta\;and\;\gamma- cyclodextrins$ were prepared and suspended to enhance the bioavailability of piroxicam. A quantitative analysis was employed HPLC for the determination of piroxicam in the rabbit serum after a single oral dose in suspension of piroxicam and each of inclusion complexes of piroxicam with $\alpha,\;\beta\;and\;\gamma- cyclodextrins$, respectively. The bioavailability and serum level of piroxicam exhibited the highest in piroxicam clathrated $\beta-cyclodextrin$ than both piroxicam and the other complexes administered. and the total area under the curve of serum concentration versus time for their inclusion complexes were larger than that of piroxicam.

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