• Journal of the Korean Wood Science and Technology
• /
• v.36 no.4
• /
• pp.26-36
• /
• 2008

As the height of the light-framed building increases, the lateral load and overturn-moment are increased and the possibility of the building overturn becomes larger. Because the shear wall resists lateral load in light-framed building, the reinforcement of shear wall is required. In order to reinforce the light-framed shear wall, using lag screw fastener type (B-HD) and using bolt type (S-HD) hold-down connectors were applied for test. And domestic larch lumbers, $38{\times}140mm$ and $89{\times}140mm$, KS 2nd grade, were used for the stud. The North American OSB panels were used for sheathing panel. Static loads, load speed 6 mm/min, were applied on top of the wall. As a result, shear strength of the wall that using hold-down connector was improved sufficiently. And when applying the S-HD type hold-down connector, stud should be reinforced against weakening by drilled hole. As increasing the number of lag screw, the number of bolt and the product allowable strength, the strength of shear wall that using hold-down connector was also increased. When applying hold-down connector to light-framed building using 38 mm stud, it must be reinforced by enlarging the thickness of stud like as 38 mm doubled column.

• Korean Journal of Microbiology
• /
• v.31 no.4
• /
• pp.279-285
• /
• 1993

Alterations of the p53 gene arc among the most frequent genetic changes in human cancer and often result in increased levels of p53 protein within the malignant cells. Detection of accumulated p53 protein can be a useful prognostic tool in human cancer. In order to make polyclonal antibodies for immunohistochemical screening. human p53 gene was expressed in E. coli in the form of GST (glutathione S-transfi.:rase) fusion proteins. Two p53 gene fragments. which were N('()I small fragment encoding amino acid residues of 1-151-: and Ncol large fragment of 159-393. were subeloned into the unique BamHI site present within the pGEX-2T vector using BamHI linker and recombinant plasmids pGTNS and pGTNL were constructed. respectively. The p53 cDNA fragment (from pC53-$SN_3$,) encoding amino acid 38-145 (proline at residue 72) was amplified by polymerase chain reaction(PCR). The amplified DNA was digested with BamHI and Prull and inserted into the BamHI-Smal sites of pG EX-2T and recombinant plasmid pGTBP was constructed. After IPTG induction of these plasmids for 4 hours. fusion proteins were purified from E. coli extracts with glutathione Sepharose beads. The bound proteins were resolved by 10% SDS-polyacrylamide gel electrophoresis and the molecular weights were 54 kDa. 53 kDa and 40 kDa. respectively. Approximately one milligram of fusion proteins were purified from 1 -liter cultures.

• Journal of Conservation Science
• /
• v.34 no.5
• /
• pp.379-385
• /
• 2018

This study examined the condition of degraded plastic artifacts and conducted materials assessments for conservation treatment. The properties and bond characteristics of five types of adhesives(EVA, PVAc, Cyanoacrylate) were evaluated to select the adhesive most suitable for conservation treatment. The degradation of plastic artifacts caused cracks, peelings, and surface warping. According to the FT-IR analysis, the synthetic resin used in the artifact was estimated to EVA type. The properties of the adhesives were evaluated through acidity, and hardness measurements. The results showed that acidity varies with the type of adhesive, and that the hardness of PVAc was higher than those of EVA. On comparing the bond characteristics of the samples after artificial degradation, it was found that PVAc and Cyanoacrylate increased the chromaticity and hardness of the samples, while EVA was the most stable with relatively little change. Thus, EVA was confirmed to be the most suitable materials for conservation treatment of degraded plastic artifacts. In this study, as the experiment of treatment materials based on actual artifact, it is expected that it could be used a basis research for conservation treatment of plastic artifacts.

• Economic and Environmental Geology
• /
• v.15 no.1
• /
• pp.41-47
• /
• 1982

Typical stratigraphic sequences of Paleozoic form the Baegunsan Syncline (equal to Hambaeg Syncline) and Taebaegsan Group is the basement in the southern part of Kangweon Province. Deformation pattern depends on their stratigraphic site and their internal properties. In the biotite schists and meta-sandstones of Pre-Cambrian sequences, flow folds and ptygmatic folds are developed, and high strain deformation is pervasive. Deformational patterns of Cambro-Ordovician are variable because of their various formations. Fracturing is pervasive in the Jangsan Quartzite and Daegi and Maggol Limestone. Welldeveloped slaty cleavage and angular folds of kink and chevron types are the prevailing structures of pelitic rocks. The most characteristic feature of limestone alternated with argillite is the "Compositional cleavage (Author's proposal)" known as the "Worm-eatern" structures. It was known that this structures have a sedimentary origin. But their preferred orienation of long axis of erosional lime holes, originated from tectonic deformation of folding. And this structures have the same character as the axial plane cleavages. Fracturing and long wave concentric folds are dominant characters of the Upper Paleozic sequences. In this area, two folds are superimposed. Field studying and analysis of structures show that the cross-folds of NS-direction are pervasive and were deformed by the EW-directional Baegunsan Syncline. The cross-folding is the major and penetrative deformation and prior to Baegunsan Syncline.

• Korean Journal of Environmental Biology
• /
• v.32 no.4
• /
• pp.382-388
• /
• 2014

Shortfin eel (Anguilla bicolor pacifica) is a species of commercial importance and its production is greatly affected due to the infection by Heterosporis anguillarum. In this study, we evaluated the effect of H. anguillarum infection on the growth of Shortfin eel. A disease that trunk muscle of cultured shortfin eel, Anguilla bicolor pacifica, were irregular and resulted in death, breakout of the commercial eel culture farm. We observed that the trunk muscle of infected eels were irregular and represented white or yellowish externally. Histopathologically, a great numbers of large or small spores and sporophorocysts were also observed in degenerated muscle layer. The cloning of specific gene of H. anguillarum, encoding small subunit ribosomal RNA (SSU-rRNA) was amplified by the polymerase chain reaction(PCR) from the muscle lesion of diseased eel. The size of clone gene is well matched with the size of small subunit ribosomal RNA of H. anguillarum and thus confirming the infection by H. anguillarum.

• The Journal of Engineering Geology
• /
• v.17 no.4
• /
• pp.601-613
• /
• 2007

It is reported that factors affecting the behavior of reinforced earth retaining walls built on soft ground are not only basic physical properties but also the increase of load by the reinforced earth retaining walls, consolidation period, pore water pressure, etc. This study analyzed the behavior of reinforced earth retaining walls and soft ground using SAGE CRISP, a ground analysis program. First, we examined the effect of the replacement method, which was to prevent the excessive displacement of reinforced earth retaining walls, in improving the behavior of the walls. Second, we compared and analyzed how the behavior of ground is affected by the vertical interval of stiffeners on the back of reinforced earth retaining walls after the application of the replacement method. Lastly, we proposed the optimal replacement width and depth in the application of the replacement method. The results of this study proved that the replacement method is considerably effective in improving the behavior of reinforced earth retaining walls. In addition, the vertical interval of stiffeners on the back of reinforced earth retaining walls appeared effective in improving the horizontal displacement of the top of retaining walls but not much effective in improving the vertical displacement of the back of retaining walls. In addition, improvement in horizontal-vertical displacement resulting from the increase in replacement width was not significant and this suggests that the increase of replacement width is not necessary. With regard to an adequate replacement depth, we proposed the ratio of replacement depth to the height of retaining walls(D/H) according to the ratio of the thickness of the soft layer to the height of retaining walls(H/T).

• Applied Microscopy
• /
• v.39 no.1
• /
• pp.65-72
• /
• 2009

Cryo-SEM which enables specimens to be observed in frozen form has been used to study liquid specimens in their native states. Cryo-methods, sample preparation for cryo-SEM, are quite complex and involve several discrete but vitally interconnected steps which are rapid cooling, fracturing, sectioning, etching and coating. It is important to select practical techniques and to optimize conditions of each steps considering analytic purpose and specimen characters, viz., sample dimension, water contents. In this study, etching methods and sample preparation before freezing had been studied for observation of cyanobacteria, Synechocystis sp. PCC 6803 using cryo-SEM and their cryo-SEM images were compared to Conventional SEM (CSEM) images treated by chemical fixation. We could observe the improved morphological images of the pili of the surface and membranes of Synechocystis sp. PCC 6803 and the three-dimensional architectures of their biofilm, which were difficult to observe using chemical fixation and CSEM. These results suggest that cryo-methods/cryo-SEM are useful techniques for morphological study of biological specimen.

• Clinics in Shoulder and Elbow
• /
• v.16 no.2
• /
• pp.123-129
• /
• 2013

Purpose: The purpose of this study is to compare the clinical and radiological outcomes of two surgical treatments, transacromial pin fixation and hook plate fixation, for unstable distal clavicle fractures. Materials and Methods: Twenty four patients with Neer type II distal clavicle fractures who underwent surgery with transacromial pin fixation (Group I: 12 patients) and hook plate fixation (Group II: 12 patients) were included. Reduction and union were evaluated using plain radiographs. Functional evaluation was performed according to UCLA score and Constant-Murley score at last follow-up. Results: All 24 cases showed bone union. Complete union took an average of 10 weeks for group I and 11.7 weeks for group II. Average UCLA score was 33 (group I) and 32.8 (group II). Average Constant score was 88.5 (group I) and 88.8 (group II). No significant difference for each variable was observed between the two groups. Conclusion: For the surgical treatment of Neer type II distal clavicle fractures, transacromial pin fixation and hook plate fixation are both useful methods.

• Applied Chemistry for Engineering
• /
• v.17 no.6
• /
• pp.604-608
• /
• 2006

Polymer composites for measuring the radioactive contamination are prepared by coating ZnS(Ag) powders as a scintillator on polysulfone base layer. The composites consist of the active layer for a scintillation reaction with radioactive wastes and the transparent support layer for transmittance of light photons emitted by scintillation in the active layer. The binding of the active layer, including ZnS(Ag), on the support layer is proceeded via coating with polysulfone as a binder, without any extra adhesive. The coating was obtained by either casting via a Doctor Blade as applicator or screen printing. The prepared composites feature a monolithic structure, resulting in the complete adhesion between two layers. The composite prepared by the casting technique using an applicator holds a good detection efficiency in measuring the alpha radionuclide, but its structure becomes fragile because of warping in morphology. On the contrary, the composite prepared by the screen printing shows a good detection capacity as well as a good stability in a mechanical shape.

• Journal of the Korean Geotechnical Society
• /
• v.27 no.12
• /
• pp.17-25
• /
• 2011

A fabric of soil media may change due to certain factors such as dissolution of soluble particles, desiccation, and cementation. The fabric changes affect the mechanical behavior of soils. The purpose of this study is to investigate the effects of geo-material dissolution on shear strength. Experiments and numerical simulations are carried out by using a conventional direct shear and the discrete element method. The dissolution specimens are prepared with different volumetric salt fraction in sand soils. The dissolution of the specimens is implemented by saturating the salt-sand mixtures at different confining stresses in the experimental study or reducing the sizes of soluble particles in the numerical simulations. Experimental results show that the angle of shearing resistance decreases with the increase in the soluble particle content and the shearing behavior changes from dilative to contractive behavior. The numerical simulations exhibit that macro-behavior matches well with the experimental results. From the microscopic point of view, the particle dissolution produces a new fabric with the increase of local void, the reduction of contact number, the increase of shear contact forces, and the anisotropy of contact force chains compared with the initial fabric. The shearing behavior of the mixture after the particle dissolution is attributed to the above micro-behavior changes. This study demonstrates that the reduction of shearing resistance of geo-material dissolution should be considered during the design and construction of the foundation and earth-structures.