• Title/Summary/Keyword: 효소공정

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A case Study on One-Step Bio-treatment including cellulase (셀룰라아제를 포함한 One-Step Bio-treatment 연구 적용사례)

  • Kim, Moon-Jung;Yoon, Min-Sun;Lee, Jung-Ho
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2012.03a
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    • pp.94-94
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    • 2012
  • 섬유산업은 원가 경쟁력 향상과 산업 고도화에 필요한 미래형 저에너지 염색가공 핵심 기술을 통한 고부가가치 섬유제품의 창출과 섬유산업의 선진화달성에 꾸준히 노력을 해 왔다. 염색가공업의 에너지 소비는 섬유산업에서 염색가공업이 연료 사용량의 77%, 전기사용량의 54%를 차지하여 섬유산업의 에너지절감을 위해서는 염색가공 공정에서의 에너지 절감이 가장 중요하다. 국내 염색가공 분야의 4백여 업체를 대상으로 실시한 애로 기술에 관한 설문 조사결과에 따르면 염색공업의 에너지 절약형 구조로의 전환을 위해서 가장 시급히 요구되는 우선기술 1순위는 에너지절약형 염색가공 공정기술로 에너지 절감의 필요성을 반영하고 있다. 효소(Enzyme)가 섬유산업에 도입되기 시작한 것은 면섬유의 호발에 아밀라제가 사용되기 시작하면서 부터이며, 최근 유럽선진국들의 강화된 환경규제와 눈부신 바이오테크놀러지(Biotechnology)의 발전에 기인하여 섬유산업에서 관심과 적용이 확대되고 있다. 섬유산업에서 효소적용의 장점은 효소 그 자체가 자연산물이기 때문에 생분해 되고 중성에 가까운 pH에서 반응하므로 처리액이 환경문제를 일으키지 않으며, 기질특이성을 가져 매우 선택적으로 반응하여 부반응으로 인한 섬유의 손상을 최소화 하는 효과를 들 수 있으며, 무엇보다 소량 저온반응으로 인한 에너지절감이 가장 큰 장점이라 할 수 있다. 실제 섬유산업에서는 전분호제를 제거하는 아밀라아제, 데님워싱 및 면섬유의 후가공에 사용하는 셀룰라아제, 표백 후 잔류하는 과산화수소를 제거하는 카탈라제 등을 적용하는 사례가 많아지고 있으며, 이 밖의 다양한 공정에 효소 이용연구가 활발히 진행되고 있다. 본 연구에서는 그 동안 섬유 업체에서 직물의 표면 잔털을 제거하여 매끄러운 외관과 선명한 색상을 재현하기위해 후가공으로 셀룰라아제를 이용했던 공정을 개선하여, 효소를 이용한 정련-Bio polishing-염색의 3공정을 1욕처리를 통해 기존 단독으로 진행되어진 기존제품과의 중량, 외관, 색상재현성, 정련성 등을 비교하여 에너지 절약형 공정기술의 효과를 극대화 해보았다.

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Optimization of enzymatic hydrolysis of legs proteins of black body fowl(Ogae) to produce peptides using a commercial protease (단백질 분해효소를 이용한 오계 다리육 펩타이드 생산 최적화)

  • Choi, So Young;Kim, A-Yeon;Yoo, Sun Kyun
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.1
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    • pp.176-185
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    • 2016
  • Yeonsan Ogae has been known as supporting health and high efficacy of treatment. In recent days, as the efficacy of functional peptides has known, the optimization of oligo peptides production and its characteristics from Ogae legs has been performed. Response surface method was used to perform the optimizaion of enzyme hydrolysis. The range of processes was temperature ( 40, 50 and $60^{\circ}C$), pH( pH 6.0, 7.0 and 8.0 ), and enzyme( 1, 2 and 3% ). The degree of hydrolysis, amino acids, molecular weight of products were analyzed. The optimum process of enzyme hydrolysis were determined as temperature $58^{\circ}C$, pH 7.5, and enzyme concentration 3%. At optimum conditions, the degree of hydrolysis after 2 h reaction was 75-80%. The amino acid and were 168.131 mg/100 g, respectively. The molecular weight of products by using MALDI-TOF was ranged from 300 to 1,000 Da.

Hydrolysis of Cellulose by Immobilized Cellulase in a Packed Bed Reactor (충진층 반응기에서 고정화 cellulase에 의한 셀룰로스 가수 분해)

  • Kang, Byung Chul;Lee, Jong Baek
    • Journal of Life Science
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    • v.23 no.11
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    • pp.1365-1370
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    • 2013
  • Immobilized cellulase on weak ion exchange resin showed a typical Langmuir adsorption isotherm. Immobilized cellulase had better stability with respect to pH and temperature than free cellulase. Kinetics of thermal inactivation on free and immobilized cellulase followed first order rate, and immobilized cellulase had a longer half-life than free cellulase. The initial rate method was used to characterize the kinetic parameters of free and immobilized enzyme. The Michaelis-Menten constant $K_m$ was higher for the immobilized enzyme than it was for the free enzyme. The effect of the recirculation rate on cellulose degradation was studied in a recycling packed-bed reactor. In a continuous packed-bed reactor, the increasing flow rate of cellulose decreased the conversion efficiency of cellulose at different input lactose concentrations. Continuous operation for five days was conducted to investigate the stability of long term operation. The retained activity of the immobilized enzymes was 48% after seven days of operation.

Optimization of Proteolytic Enzyme Treatment for the Production of Spirulina Extract (단백질 분해 효소를 이용한 스피루리나 추출물 제조 공정 최적화)

  • In, Man-Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.9 no.2
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    • pp.550-555
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    • 2008
  • An efficient production method of spirulina extract was developed by enzymatic treatment using proteolytic enzymes. The suitable dosage of Tunicase, a cell lytic enzyme, was used to be 2.0% (w/w). To maximize solid recovery and spirulina extraction (SE) index, which indicates nucleic acid-related substances content, the dosage of Alcalase, commercially available pretense, was found to be 1.0% (w/w). By simultaneous treatments using optimal dosages of Tunicase and Alcalase, the highest SE index and solid recovery were obtained. The SE index and solid recovery of simultaneous treatments were notably enhanced by 100% ($11.4%\;{\rightarrow}\;22.8%$) and 56% ($45.2%\;{\rightarrow}\;70.7%$), respectively, than those of the non-treated extracts.

The Effect of Acid Hydrolysis and Enzymatic Saccharification in Bioethanol Production Process Using Fruit Peels (과일껍질을 이용한 바이오에탄올 생산 공정에서 산 가수분해 및 효소당화의 영향)

  • Lee, Seung Bum;Kim, Hyungjin
    • Applied Chemistry for Engineering
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    • v.25 no.6
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    • pp.619-623
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    • 2014
  • The acid hydrolysis and enzymatic saccharification were carried out for the production of cellulosic ethanol. The possibility of bio-energy production from tangerine peel and apple and watermelon rind was evaluated by determining the optimum production condition. The optimum conditions for the production of cellulosic ethanol from fruit peel were as follows: the sulfuric acid concentration and reaction time of acid hydrolysis for the ethanol production from an apple rind were 20 wt% and 90 min, respectively. The concentration of sulfuric acid for tangerine peel and a watermelon rind at the hydrolysis time of 60 min were 15 wt% and 10 wt%, respectively. A viscozyme was proven as the best conversion for the ethanol production when using enzymatic saccharification from fruit peels. The optimum enzymatic saccharification time for tangerine peel and apple and watermelon rind were 60, 180, and 120 min, respectively.

The Optimum Condition of SSF to Ethanol Production from Starch Biomass (전분질계 바이오매스의 동시당화발효 조건 최적화)

  • Na, Jong Bon;Kim, Jun Seok
    • Korean Chemical Engineering Research
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    • v.46 no.5
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    • pp.858-862
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    • 2008
  • The Simultaneous Saccharification and Fermentation(SSF) of ethanol production from potato starch studied with respect to growth pH, temperature, substrate concentration. The glucoamylase and Saccharomyceses cerevisiae have a capacity to carry out a single stage SSF process for ethanol production. The characteristics, termed as starch hydrolysis, accumulation of glucose, ethanol production and biomass formation, were affected with variation in pH, temperature and starch concentration. The maximum ethanol concentration of 12.9g/l was obtained using a starch concentration 30g/l, which represent an ethanol yield of 86%. The optimum conditions for the maximum ethanol yield were found to be a temperature of 38, pH of 4.0 and fermentation time of 18hr. Thus by using the control composite design, it is possible to determine the accurate values of the fermentation parameters where maximum production of ethanol occurs.

Development of (α-Amylase Coated Magnetic Nanofiber for the Hydrolysis of Starch. ((α-Amylase가 고정화된 Magnetic Nanofiber를 이용한 전분 분해공정 개발)

  • Kim, Hyun;Lee, Jung-Heon
    • Journal of Life Science
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    • v.17 no.9 s.89
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    • pp.1260-1265
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    • 2007
  • Magnetically separable enzyme-coated nanofibers were developed for the hydrolysis of starch. Stability of ${\alpha}-amylase-coated$ nanofiber was greatly improved and its residual activity was maintained over 92.7% after 32 days incubation at room temperature and under shaking conditions (200 rpm). The recovery of enzyme was high and enzyme activity after 10 recycle was 95.2% of its original activity. Developed enzyme-coated nanofibers were used for the hydrolysis of starch. When 0.5 mg of magnetically separable enzyme nanofibers was used, 40 g/l of starch (2 ml) was completely degraded within 40 min. The continuous enzyme reactor was developed and used for starch hydrolysis and 76% of starch (30 g/l) was hydrolyzed with 1 hr residence time.

Development of On-line Monitoring Techniques for Fumaric Acid and Succinic Acid by Flow Injection Analysis (흐름주입분석기술에 의한 푸마르산과 숙신산의 모니터링 기술 개발)

  • 손옥재;김춘광;이종일
    • KSBB Journal
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    • v.18 no.5
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    • pp.377-384
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    • 2003
  • On-line monitoring techniques for fumaric acid and succinic acid were developed by flow injection analysis (FIA). For the determination of fumaric acid, two enzymes, fumarase and malic dehydrogenase were immobilized on VA-epoxy Biosynth E3-carrier and integrated into a FIA-system with a fluorescence detector. For the analysis of succinic acid, isocitrate lyase and isocitrate dehydrogenase were also immobilized on VA-epoxy polymer support and used in a FIA system. The immobilized enzymes in two FIA systems were characterized systematically, e.g. optimum pH and temperature, inhibitory effects etc. Two FIA systems were also used to on-line monitor the concentrations of fumaric acid and succinic acid in biotechnological processes. Good agreement between on-line monitored data and off-line data measured by HPLC showed extensive application of the FIA systems in bioprocesses.

축산물의 저알레르기화 처리

  • Lee, Bu-Ung;Heo, Mun-Yeong;Jang, Un-Gi;Kim, Tae-Hwa
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2004.10a
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    • pp.1-29
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    • 2004
  • 본 연구에서 저알레르기 처리 공정은 Autoclaving, 가열처리, micro wave, dry heating, 초음파, 효소, 인산염, 천연효소, 가용화, 복합처리 등의 처리 공정으로 하였다. 저 allergy처리에서 allergy가 완전히 억제되는 것은 가열처리를 한 것으로 쇄양 B(추출액+가열 3분), autoclave 처리, micro파 처리, dry heating처리, 복합처리를 했을 때이다. 또한 천연효소(키위)를 침지한 후 tolergen과 같이 3분간 가열했을 때 allergy가 억제되는 것으로 나타났다. 즉 가열처리로 인한 단백질 구조 변성으로 이러한 결과를 보인 것으로 보인다. 인산염의 경우도 어느 정도 억제가 되는 것으로 보이고 있다. 나머지 처리들은 거의 효과를 보이고 있지 않다. 천연효소와 tolergen(쇄양)을 그냥 처리했을 때에는 allergy 억제효과는 없는 것으로 나타났다. 우유의 저 allergy 처리는 효소, autoclave, micro 파, NaOH 처리, 복합처리에서 감소되는 것으로 나타났다. 이러한 결과는 western blotting으로도 확인되었으며 그 억제율 %은 식육에서는 상당한 가열처리를 통하여 알레르기를 감소시킬 수 있는 것을 볼 수 있으며 또한 인산염, 가용화(NaOH처리)도 저 알레르기 효과가 있음을 알 수 있다. 키위, 쇄양 단독 처리 시 저 알레르기 효과가 없지만 약간의 가열을 통하여 알레르기가 감소됨을 알 수 있다. 우유는 효소나 autoclave 처리만이 저 allergy 효과가 각각 28%, 45%로 적게 나타났다. 모든 복합처리의 경우에서는 그 억제율이 41-96%로 높은 효과가 있음을 알 수 있다. 천연효소처리와 인산염 처리된 식육의 전자현미경적 관찰은 control과 비교시 조직의 변화가 없고 둘다 근육 단백질 구조를 분산시키는 것으로 나타났다. 또한 고기를 단계별 복합처리로 저 allergy 처리는 단계별로 점차적으로 allergy가 감소되었다. 즉 단계별로 억제가 안되는 것부터 억제되는 처리를 복합적으로 처리한 것으로 그 단계는 천연효소처리에 인산염 처리, 여기에 초음파 처리, 마지막 단계로 3분 끓이면 억제율이 68%까지 억제되었다. 이는 단일처리시 전혀 억제를 못하는 처리를 단계별로 한 단계씩 더해가면 allergy 억제효과가 나타난다고 할 수 있겠다. 초음파 처리도 역시 저 allergy 처리 공정에 이용될 수 있는데 이것은 그 처리로 인해 새로운 알러젠이 생성될 수도 있다. 또한 복합처리로 allergy를 감소시키면 연속적이고 동시적으로 하기 때문에 원가를 절감할 수 있다.

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