• Journal of Animal Science and Technology
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• v.55 no.1
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• pp.41-49
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• 2013

In vitro and in situ incubation studies were conducted to determine effects of yeast culture supplements (Saccharomyces cerevisiae) on cellulolytic bacterial function and fiber digestion in rice straw. In vitro dry matter digestibility of rice straw gradually increased according to supplemental levels of yeast culture (0.0, 0.2, 0.4, 0.6, 0.8 and 1.0%). Digestibility of rice straw started to increase apparently when yeast culture was added more than 0.6% level (p<0.05). Also, we reconfirmed that in vitro dry matter digestibility was significantly increased by 0.6% of yeast culture addition in 4% NaOH treated and non-treated rice straws (p<0.05). When in situ dry matter digestibility was tested in Korean native goats fed basal diet or experimental diet which contained 1.0% of yeast culture, the yeast culture feeding improved in situ dry matter digestibility in both 4% NaOH treated and non-treated rice straws (p<0.05). In case of real-time PCR monitoring cellulolytic bacterial function, the bacterial population attached on rice straw showed the increasing trends with higher level of yeast culture spraying on rice straw. F. succinogenes and R. flavefaciens were significantly increased in accordance to spraying levels of yeast culture (0.0, 0.1 and 0.3%) at both 12 and 24 hrs of in situ incubation (p<0.05). R. albus was significantly higher population in yeast culture spraying than non-soraying at 12 hrs of in situ incubation (p<0.05). These bacterial populations were showed the increasing trends with digestibility enhancement of rice straw according to the higher levels of yeast culture supplement. Overall, these results clearly suggest that the presence of yeast culture result in noticeable increase of rice straw digestion, which is modulated via good effect on cellulolytic bacterial attachment to fiber substrates.

• Journal of Animal Environmental Science
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• v.6 no.3
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• pp.201-209
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• 2000

Optimal conditions to utilize egg type male chicks from hatchery for cultivating yeast(Saccharomyces cerevisiae) and the effects of the yeast culture on growth of broiler chicks were investigated. The protein concentration of the spent cockerel extracts was the highest when extracted for 72 hours. Optimal water volume added to the spent cockerel chicks for the extraction was 1.5 times to the cockerel chicks weight (v/w ratio). Lipid in the extracts from the spent cockerel chicks did not affect on the yeast growth. The number of yeast cultured in the SCELP2 medium containing spent cockerel extracts and 4 % sugarcane molasses was higher by 26 % than that in the YEPD medium containing 1 % yeast extract, 2 % bacto pepton and 2 % glucose. Also the number of yeast cultured in the SBYW2 medium containing SCEP2 medium containing SCELP2 and 4 % brewer's yeast waste was increased by 8 %, compared to that in the SCELP2 medium. Body weight gain of chicks fed 4 % yeast culture supplementations cultivated in the SBYW2 medium was increased at 5 weeks by 9 %, relative to no supplementation(P<0.05). The results from this study suggest that the spent cockerel chicks can be utilized as nitrogen sources to produce yeast culture for animal feed.

• Korean Journal of Poultry Science
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• v.29 no.2
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• pp.109-116
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• 2002

The objective of this study was to investigate the effect of feeding organic acid mixture and yeast culture on the Performance and e99 quality of laying hens fur sixteen weeks. Four hundred and fifty 19-wk-old ISA Brown layers were alloted to five treatments with five replications of each. The supplemental levels of organic acid mixture(OAM) and yeast culture(YC) in the experimental diets were 0(control), OAM 0.1, 0.2%, and YC 0.1, 0.2%, respectively. Corn -Soy basal diet containing 16.0% CP and ME 2,770 kca1/kg ME. Eggs were collected and weighed every day. Eg3 Production, fred intake and fled conversion were recorded in every four weeks. Fatty acid composition of e99 Yolk, e99she11 breaking strength, thickness and Haugh unit were measured a(her every eight weeks. Egg Production and daily egg mass of birds fed 0.2% OAM and 0.1% YC tended to be higher than those of the other treatment groups. Egg weight was the highest in hens fed with 0.2% OAM treatment, but was not significantly different. Feed conversion of hens in all OAM and YC treatments was lower than those of control, but was not statistically different. Eggshell breaking strength of hens on OAM treatments tended to be higher than those of YC treatment and control. Haugh units of the birds fed OAM was significantly higher than controls (P<0.05). 599 yolk color score of OAM treatment was higher than those of YC treatments and control, but the difference was not significant. At 27-wk-old, arachidonnic acid content in eggs from the birds fed 0.1% OAM diet was significantly higher(P<0.05) than controls. The results of this experiment indicated that dietary organic acid mixture and yeast culture tended to improve the egg Production and internal egg quality.

• Journal of Animal Science and Technology
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• v.45 no.1
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• pp.41-48
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• 2003

Two experiments were conducted to investigate the effect of dietary supplementation of yeast culture on the performance of broiler chicks and noxious gas emission in broiler houses. Two hundred forty and three hundred and twenty, one day old Cobb male broiler chicks in Expt 1 and Expt 2, respectively were alloted to four treatment levels of yeast culture (0, 0.1, 0.2, 0.4%). To each treatment, 60 birds were assigned in Expt 1 and 5 replicates of 16 birds each were assigned in Expt 2. Basal diets contained 21.5% and 19.0% CP, and 3,100kcal/kg ME for the starting and finishing periods, respectively. Ammonia and $CO_2$ gas emission were detected twice a day for seven days during the five week period of Expt 1. Weight gain, feed intake and feed efficiency were measured for five weeks in Expt 2. Intestinal microbes, blood cholesterol and ND antibody titer were examined at the end of Expt 2. In Expt 1, the concentration of $NH_3$ in the house of birds fed yeast culture tended to be lower than the control. It was significantly lower in the 0.4% yeast culture treatment than the control (P<0.05). $CO_2$ concentration was significantly lower in all yeast culture treatments regardless of its dietary supplemental level than the control (P<0.05). different from others. Feed efficiency (feed/gain), however, was significantly improved in all yeast culture treatments relative to that of the control for starting period (P<0.05) and that of 0.2% yeast culture treatment was significantly lower than those of the control and 0.4% for the overall period. Total number of E. coli in the ileum of birds fed yeast culture at 0.1 and 0.2% was significantly lower than those of the control and 0.4% in the ileum. The CFU of Lactobacillus spp. of birds fed yeast culture at 0.1% was higher in the cecum compared to other treatments (P<0.05). Total cholesterol level of chicks fed 0.1% yeast culture seemed to be lower compared to that of other treatments, whereas LDL-cholesterol level was significantly lower than those of the control and 0.4% treatment. ND antibody titer tended to be higher in the yeast culture treatments than the control, but was not significantly different. The results of these experiments indicated that 0.2% yeast culture may have a potential to reduce the noxious gas emission in broiler houses and maximize the performance of broiler chicks.

• KOREAN POULTRY JOURNAL
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• v.23 no.9 s.263
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• pp.155-157
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• 1991

• KOREAN POULTRY JOURNAL
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• v.28 no.2 s.316
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• pp.179-185
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• 1996

• Journal of Animal Environmental Science
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• v.7 no.1
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• pp.21-28
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• 2001

Optimal conditions for utilizing the slaughtered porcine blood and liver for yeast culture and the effects of the yeast cultures on the growth of broiler chicks were investigated. The quantity of yeast cultured for 24hours in the BSG medium containing blood extracts containing 5% glucose and in the LSG medium containing liver extracts containing 5% glucose were higher by 4% and 10%, respectively, than that in the YEPD medium containing 1% yeast extracts, 2% bacto pepton and 2% glucose. Optimal concentrations of ammonium sulfate supplementation to the BSG medium to increase the quantity of yeast cultured for 24 and 48 hours were 100 mM(1.3%) and 50 mM(0.65%), respectively. The optimal pH for yeast culture in BSG medium ranged from 6 to 7. One percent supplementation of either ammonium sulfate or taurine to LSG medium increased the quantity of yeast by 18% and 9%, respectively, compared to no supplementation. The body weight of chicks fed with 2% and 4% yeast culture supplementations cultivated increased at the 4th week by 10%, with relative to no supplementation. The results from this study suggest that the slaughtered porcine blood and liver can be utilized for yeast culture which is used in animal diets.

• Journal of Animal Science and Technology
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• v.45 no.2
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• pp.219-228
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• 2003

Two experiments were conducted to investigate the influence of dietary supplementation of yeast culture on the nutrient digestibility, performance and meat quality in growing-finishing pigs. Corn-soy basal diets contained 20, 18, and 16% CP and 3,265kcal/kg ME at different growth stages. One hundred and twenty pigs(8wk-old) were allocated into four supplementation levels of yest culture (0, 0.1, 0.2, 0.4%) in Expt 1. Weight gain, feed intake and feed efficiency were periodically recorded for 112 days. A metabolic feeding trial was conducted to measure the nutrient digestibility. Physical and chemical characteristics of the Longissimus Dorsi muscle(LM) from the pigs were measured at the end of experiment (Expt 2). In Expt 1, weight gain and feed efficiency were not different among the dietary treatment groups during the overall period. The digestibilities of protein and fiber were greater in 0.1% and 0.2% yeast culture-supplemented groups than in control (P<0.05). However, fat and ash digestibilities were not improved by the dietary treatment. In Expt 2, the LM protein content in 0.1% yeast culture-supplemented group, but not in 0.2% or 0.4%-supplemented group, was greater than that in the control group (P<0.05). The LM pH, purge loss, cooking loss and color were not affected by the dietary treatment. The LM shearing force was less in 0.2% yeast culture-supplemented group than in the control group (P<0.05). Other LM physicochemical properties did not differ between the dietary treatment groups. Total saturated and unsaturated fatty acids percentages and total cholesterol content of the LM did not differ across the dietary treatment groups.

• The Korean Journal of Mycology
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• v.39 no.3
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• pp.227-228
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• 2011

Fibrinolytic activities of culture concentrates of various yeasts were investigated. The concentrates of the culture broth of Saccharomyces cerevisiae Y99-7 showed the strongest fibrinolytic activity of 25 mm (clear zone). The fibrinolytic activity of Saccharomyces cerevisiae Y99-7 was more high in the culture concentrates from PD broth rather than that of yeast extract-peptone dextrose cultures (clear zone : 22.7 mm).

• The Korean Journal of Mycology
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• v.40 no.1
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• pp.60-64
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• 2012

Physiological functionalities of culture concentrates from various fungi were investigated. The culture concentrates from Saccharomyces cerevisiae Y277-3 showed the highest tyrosinase inhibitory activity of 42.7%. Among mold physiological functionalities, the culture concentrates from Aspergillus orygae CN20-3-1-4 showed the highest antioxidant activity of 15.8%. The other functionalities of fungi were very low or not detected. The intracellular tyrosinase inhibitor from Saccharomyces cerevisiae Y277-3, which showed the highest physiological functionalities was maximally produced when the strain was cultured in PD broth at $30^{\circ}C$ for 24 h.