• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1977.10a
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• pp.198.2-198
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• 1977

1) 목적 : Glutathione은 천연에 미양으로 분포하고 있으며 의약용으로도 매우 중요하기 때문에 효모에 의한 glutathione생산에 관한 보고가 많지만 glutathione을 다대 축적하는 효모 및 배양조건에 관한 보고는 적다. 본 실험에서는 glutathione을 다량 생산하는 효모의 screening과 배양조건을 검토하여 보고 하고자 한다.(중략)

• Journal of Animal Environmental Science
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• v.6 no.3
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• pp.201-209
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• 2000

Optimal conditions to utilize egg type male chicks from hatchery for cultivating yeast(Saccharomyces cerevisiae) and the effects of the yeast culture on growth of broiler chicks were investigated. The protein concentration of the spent cockerel extracts was the highest when extracted for 72 hours. Optimal water volume added to the spent cockerel chicks for the extraction was 1.5 times to the cockerel chicks weight (v/w ratio). Lipid in the extracts from the spent cockerel chicks did not affect on the yeast growth. The number of yeast cultured in the SCELP2 medium containing spent cockerel extracts and 4 % sugarcane molasses was higher by 26 % than that in the YEPD medium containing 1 % yeast extract, 2 % bacto pepton and 2 % glucose. Also the number of yeast cultured in the SBYW2 medium containing SCEP2 medium containing SCELP2 and 4 % brewer's yeast waste was increased by 8 %, compared to that in the SCELP2 medium. Body weight gain of chicks fed 4 % yeast culture supplementations cultivated in the SBYW2 medium was increased at 5 weeks by 9 %, relative to no supplementation(P<0.05). The results from this study suggest that the spent cockerel chicks can be utilized as nitrogen sources to produce yeast culture for animal feed.

• Journal of Animal Science and Technology
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• v.55 no.1
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• pp.41-49
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• 2013

In vitro and in situ incubation studies were conducted to determine effects of yeast culture supplements (Saccharomyces cerevisiae) on cellulolytic bacterial function and fiber digestion in rice straw. In vitro dry matter digestibility of rice straw gradually increased according to supplemental levels of yeast culture (0.0, 0.2, 0.4, 0.6, 0.8 and 1.0%). Digestibility of rice straw started to increase apparently when yeast culture was added more than 0.6% level (p<0.05). Also, we reconfirmed that in vitro dry matter digestibility was significantly increased by 0.6% of yeast culture addition in 4% NaOH treated and non-treated rice straws (p<0.05). When in situ dry matter digestibility was tested in Korean native goats fed basal diet or experimental diet which contained 1.0% of yeast culture, the yeast culture feeding improved in situ dry matter digestibility in both 4% NaOH treated and non-treated rice straws (p<0.05). In case of real-time PCR monitoring cellulolytic bacterial function, the bacterial population attached on rice straw showed the increasing trends with higher level of yeast culture spraying on rice straw. F. succinogenes and R. flavefaciens were significantly increased in accordance to spraying levels of yeast culture (0.0, 0.1 and 0.3%) at both 12 and 24 hrs of in situ incubation (p<0.05). R. albus was significantly higher population in yeast culture spraying than non-soraying at 12 hrs of in situ incubation (p<0.05). These bacterial populations were showed the increasing trends with digestibility enhancement of rice straw according to the higher levels of yeast culture supplement. Overall, these results clearly suggest that the presence of yeast culture result in noticeable increase of rice straw digestion, which is modulated via good effect on cellulolytic bacterial attachment to fiber substrates.

• Korean Journal of Food Science and Technology
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• v.29 no.5
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• pp.1021-1027
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• 1997

We examined some properties of yeast cell wall lytic enzyme produced by Dicyma sp. YCH-37. Several metal ions, reducing reagents, and chemical modifiers have little effects on the lytic activity, except guanidine-HCl. Yeast cells of early log phase were more susceptible to the enzyme than those of stationary phase, and heat-treated cells were more easily lysed than intact living ones. Yeast cells pretreated with organic solvents such as butanol and acetone were more susceptible to the enzyme than intact living ones. Yeast cells cultured in Yeast extract-Malt extract medium containing 0.5 M ammonium sulfate were easily lysed by the lytic enzyme, and yeast cells cultured without shaking were more easily lysed by the enzyme than those with shaking. When SDS, ${\beta}-mercaptoethanol$, Triton X-100, sodium sulfite, and KCl were added to enzyme reaction mixture each, lysis of yeast cells was more effective.

• Journal of Animal Environmental Science
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• v.7 no.1
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• pp.21-28
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• 2001

Optimal conditions for utilizing the slaughtered porcine blood and liver for yeast culture and the effects of the yeast cultures on the growth of broiler chicks were investigated. The quantity of yeast cultured for 24hours in the BSG medium containing blood extracts containing 5% glucose and in the LSG medium containing liver extracts containing 5% glucose were higher by 4% and 10%, respectively, than that in the YEPD medium containing 1% yeast extracts, 2% bacto pepton and 2% glucose. Optimal concentrations of ammonium sulfate supplementation to the BSG medium to increase the quantity of yeast cultured for 24 and 48 hours were 100 mM(1.3%) and 50 mM(0.65%), respectively. The optimal pH for yeast culture in BSG medium ranged from 6 to 7. One percent supplementation of either ammonium sulfate or taurine to LSG medium increased the quantity of yeast by 18% and 9%, respectively, compared to no supplementation. The body weight of chicks fed with 2% and 4% yeast culture supplementations cultivated increased at the 4th week by 10%, with relative to no supplementation. The results from this study suggest that the slaughtered porcine blood and liver can be utilized for yeast culture which is used in animal diets.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1979.10a
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• pp.246.3-247
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• 1979

우유를 원료로하여 만든 치-즈나 발효유에는 Streptococcus나 Lactobacillus와 같은 유산균이 주로 사용되는데 이러한 제품에 발효가 많이 존재한다는 것이 보고되어 있으나, 그 존재의의에 대해서는 아직 불명한 점이 많다. 따라서 본 실험에서는 발효유에 존재하는 효모의 역활을 검토하기 위하여, 발효유제품의 starter로 사용되는 Lactobacillus와 효모 Saccharomyces fragillis 그리고 Deba-ryomyces hansenii 사이에 어떠한 상호작용이 있는지 를 알아보았다. 본 실험에 사용한 유산균수는 L.casei와 L. bulgaricus를 시판발효유제품에서 분리하였고 효모균수는 당학과 보존중인 D. hansenii, S. fragilis를 사용하였다. 유산균과 효모에 대한 단독배양과 혼합배양을 실시하고, 온도와 배양기간중에 나타나는 생균수와 산도변화를 관찰하였다. 유산균의 생균수 측정에는 B.C.P agar, 효모는 Y.M agar를 사용하였다. 산도는 일반분석법에 준하여 실시하였다.

• Journal of the Korean Applied Science and Technology
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• v.32 no.1
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• pp.93-99
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• 2015

The aim of this study was to analyze nutritional compositions of rapeseed pollen granules and to determine the possible usage of pollen granules as a yeast culture medium. Rapeseed pollen granules (per 100 g) were consisted of carbohydrate 58.9 g, protein 20.8 g, fat 4.1 g, ash 2.5 g and water 13.7 g. And fructose (13.7 g), glucose (11.1 g), and sucrose (6.6 g) of sugars and K (606.7 mg) and P (603.3 mg) of minerals were highly contained. In addition, free amino acids such as glutamic acid (2,482.4 mg), aspartic acid (2,136.5 mg), lysine (1,648.3 mg), and leucine (1,631.1 mg) were present at a higher level. When liquid medium, which was made from cracked pollen granules (5, 10, 15, 20, 25, 30, and 40 g/L), was tested for yeast culture, liquid medium containing pollen granules over 15 g/L showed higher yeast growth than YPD medium (control). Liquid medium containing both cracked pollen granules (15 g/L) and NaCl (1 ~ 20 g/L) improved yeast growth than the liquid medium without NaCl. In addition, when yeast growth was tested on solid medium made from pollen granules (15 g/L) at $30^{\circ}C$ for 2 days, yeast colonies were equally well-formed like those grown on YPD medium. Overall, rapeseed pollen granules have potential properties on yeast growth and could be used as a primary source for yeast culture.

• Applied Biological Chemistry
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• v.48 no.4
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• pp.382-387
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• 2005

The aim of this study was to identify binding properties of germanium (Ge) in Germanium-fortified Yeast using optimum manufacturing process. The ratio of yeast cell and germanium solution was 1 : 0.5 (50%), and pH 6.5, $35^{\circ}C$ and 20 h during fermentation, and Germanium-fortified Yeast produced. In results of the XRD, NMR and FT-IR analysis, it was different adding inorganic Ge $(GeO_2)$ during fermentation process from transformed into germanium in Germanium-fortified Yeast. And germanium concentration was not shown any difference before and after in the dialysis test with SGF (simulated gastric fluids). Therefore, Germanium-fortified Yeast of Geranti made by using biosynthetic technology was considered that transformed into organic properties during fermentation process. And, this result showed that Germanium-fortified Yeast was not dissociated under SGF (simulated gastric fluids) condition because of its structural binding safety. Thus, Germanium-fortified Yeast was transformed into organic germanium during biosynthetic cultivation. It is expected that this Germanium-fortified Yeast can be applied as a new dietary functional materials for cellular immunity, recovery of injured cells and immune system, and possible anticancer activities by activation immune cells like macrophage.

• Journal of Aquaculture
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• v.7 no.4
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• pp.225-237
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• 1994

Survival and growth rates of flounder larvae fed rotifers and Artemia nauplii enriched by various bio-enrichment techniques were investigated. Enriched materials used in the study were $12\omega-yeast$, 2 commercial emulsified oil and microparticulated products. $\omega-yeast$ were added with one kinds of oils (cuttlefish liver oil, sardine oil, Alaska pollock liver oil, linseed oil) at the level of $5\%,\;15\%\;and\;25\%$. The results are as follows: 1. The growth rate of rotifers cultured with $\omega-yeast$ added $15\%$ cuttlefish liver oil was the best among 12 kinds of $\omega-yeast$ and this performance was almost the same with that of rotifers fed on Chlorella. 2. Survival and growth rates of flounder larvae fed on rotifers and Artemia nauplii enriched with $\omega-yeast$ and the other commercial products were higher than that those of larvae cultured with only Chlorella and non-enriched Artemia. The optimum contents of the cuttlefish liver oil in $\omega-yeast$ for rotifers and Artemia enrichment were $15\%\;and\;25\%$, respectively. 3. Optimum enrichment time and supply amount of $\omega-yeast$ for Artemia nauplii were 6 hr., 1.5 g/g cyst, respectively.

• 베이커리
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• no.11 s.352
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• pp.58-60
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• 1997