• Conservation Science in Museum
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• v.14
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• pp.81-89
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• 2013

This study concerns UV-Vis spectrophotometry and 3D-fluorescence spectrophotometry analysis of textile parts of blue and green tones dyed with indigo of blue tone and turmeric, gardenia, goldthread and amur cork tree of yellow tone. In order to verify whether the kinds of textiles affected analysis result of each dye, silk and cotton textile samples were produced. According to the analysis of the degree of reflection of UV-Vis spectrophotometry, unique reflection spectrum of indigo appeared regardless of the kinds of textiles when they were dyed with indigo. As for textiles of green tone, the 3D-fluorescence spectroscopic analysis result showed that unique spectrums of yellow dyes, turmeric, goldthread and amur cork tree appeared regardless of the kinds of textiles but the fluorescence spectrums of gardenia and indigo did not appear.

• Microbiology and Biotechnology Letters
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• v.37 no.3
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• pp.280-286
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• 2009

To investigate anti-phytopathogenic fungal activity of Coptis chinensis, the methanol extract and its organic solvent fractions were prepared. Using the extract and the fractions, in-vitro spore-germination inhibition and mycelial-growth inhibition activities were evaluated against Colletotrichum gloeosporioides, Phytohpthora capsici, Pyricularia grisea, Rhizoctonia solani, Botryosphaeri dothidea, Glomerella cingulata, respectively. Treatment of the methanol extract (500 mg/mL) into the spore of phytopathogenic fungi completely inhibited germinations for 5 days, except B. dothidea, and showed strong antifungal activities against P. grisea and B. cinerea, and antioomycetes activity against P. capsici. The minimal growth inhibition concentrations of the methanol extract against P. grisea, B. cinerea and P. capsici were 300, 300, and 500 mg/mL, respectively. For practical application of C. chinensis in red-pepper field, the hot-water extract (1,000 mg/mL) was prepared in commercial facility, after evaluation of heat stability and solvent-extraction yields of antifungal substances. The 3-times leaf-spray of the extract from June to August, 2008 did not show any deleterious effect to red-pepper. In fact, the leaf-spray promoted plant growth including leaf, root and fruit. The average weight and rind of each fruit were increased to 119% and 117% comparison to those of without treatments. Our results suggest that C. chinensis is a useful source for control of red-pepper diseases and plant growth.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.1
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• pp.1-10
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• 2017

Cimicifuge dahurica (C. dahurica), Coptis chinensis (C. chinensis) and Phellodendri amurense (P. amurense) extracts has an detoxification effect and Magnol obovata bark (M. obovata) extracts has an antibacterial effect in oriental medicine. This study investigated the possibility of the extract mixture as a functional cosmetic ingredient by mixing C. dahurica, C. chinensis, P. amurense and M. obovata extracts. MTT assay was carried out for toxicity test and DPPH/ABTS experiments for antioxidant effects of the extract mixture. Results show that the extract mixture was safer and antioxidant effects in a dose-dependent manner than single extract of the mixture. The mixture effectively inhibited NO (nitric oxide) production, which indicate good efficacy for anti-inflammation. The mixture also protected UVB-induced cell damage and increased type 1 pro-collagen synthesis in fibroblast. In addition, it's treatment inhibited the melanin synthesis and tyrosinase activity by lowering expression of MITF, tyrosinase and TRPs in B16F10 melanoma cell. These results suggest that medicinal herbal extract mixture may be useful as a functional ingredient for anti-aging and skin whitening cosmetic formula.

• Korean Journal of Food Science and Technology
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• v.31 no.3
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• pp.764-770
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• 1999

Antimicrobial activities were assayed through the hot-water extracts from 41 species of medicinal plants against Helicobacter pylori which is known as the ulcerogenic phathogen. Opuntia ficus-indica, Houttuynia cordata, Sinomenium acutum, and Coptis japonica showed the MIC at the concentrations less than 100 ppm, Pulsatilla koreana, Forsythia koreana, Rheum undulatum, and Perilla frutescens less than 200 ppm, Belamcanda chinensis, Arctium lappa, Cassia tora, Citrus tachibana, Siegesbeckia orientalis, and Caesalpinia sappan less than 300 ppm by the 2-fold dilution method. In disc method only three of them were confirmed to have antimicrobial activities which were increased in the order Perilla frutescens, Coptis japonica, Caesalpinia sappan. Three extracts were partitioned with chloroform, ethyl acetate and butanol in sequence and examined for the activity to inhibit H. pylori. The major ativities were observed in ethyl acetate fraction of Caesalpinia sappan, butanol fraction of Perilla frutescens, butanol and chloroform fraction of Coptis japonica. The partitioned fractions were found to have increased antimicrobial activities in all extracts. The experiments in which the extracts were added into urea R broth containing the crude urease derived from H. pylori resulted in the increase of pH and optical density at 560 nm to 8.15 and 1.7 respectively. Urease activity of H. pylori was inhibited over 80% by Caesalpinia sappan, Perilla frutescens, and Coptis japonica, of which Caesalpinia sappan suppressed up to 95%.

• Applied Chemistry for Engineering
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• v.17 no.1
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• pp.52-57
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• 2006

Coptidis Rhizoma, an antimicrobial agent from natural source, is known to have the antiviral effect on the Candida albicans that causes the infectious dermatitis. The valuable protein was extracted from the Coptidis Rhizoma, To prevent denaturalization from external stimulus and improve adsorption onto the skin, the nano-sized liposomes were prepared as a carrier. The CPR-containing liposomes showed an average diameter of 187 nm, surface charge of 3.337 mV and 33% encapsulation efficiency. The release behavior of CRP from the liposome was investigated with various temperature and releasing time. The PVA solution was coated on the surface of liposome to improve the stability. The coated liposome showed slow release behavior in comparison with the non-coated liposome. The CRP in the liposome maintained the effect on the Candida albicans after treating it at 50 and with ultraviolet for 24 h.

• Journal of Life Science
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• v.25 no.7
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• pp.748-756
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• 2015

The report explores the possibility that the single extract of natural substances and the mixture of single extracts of natural substances create a synergy effect to increase the antimicrobial activity. It also compares the previous researches to find out if the natural medicinal herbs’ extract has antimicrobial activity on dandruff causative organism, Malassezia furfur. For the experiment on the mixture of single extracts of natural substances, the results are like following: 1. Staphylococcus aures’s antibacterial activity is resistant to mixture of three natural substances. 2. Escherichia coil’s antibacterial activity is resistant to mixture with Coptidis rhizoma. 3. Candida albicans’ antifungal activity is resistant to mixture with Chinese galls, which means the different results may be expected when tested with each germ. 4. On the other hand, Malassezia furfur has no antifungal activity on the single extract of natural substances and shows weak antifungal activity, whose diameter is 3.20 mm when tested with the mixture of 50% of Coptidis rhizoma and 50% of Phytoncide. The result is totally different from the one on the same eumycetes, C. albicans. That is because M. furfur has more lipophilic chemicaled cell walls than C. albicans does and it also consists of lamella layer, inner plasma membrane and intermediate multiple layers.

• Journal of Conservation Science
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• v.26 no.4
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• pp.429-436
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• 2010

Dyestuffs extracted from fifteen kinds of natural dyeing materials were researched on their antimicrobial performance against 3 kinds of bacteria and 2 kinds of fungi in order to develop conservation/storage materials of traditional and natural dyed papers. The three types of mordants were evaluated by using the same method and then mixed with the dyestuffs to research their antimicrobial performance. With those results, dyed paper was made by mixing mordants with 9 kinds of dyestuffs with excellent antimicrobial performance. Among the dyed papers, Cotinus coggygria, Coptis chinensis, Phellodendri amurense, Rhus javanica and Acer ginnala multiple mordant papers were found to have an antibacterial quality against fungi, while, in the insect repellent experiment using termites, Coptis chinensis and Phellodendri amurense dyed papers were found to have insect repellent qualities. Also, with regard to the research result about the affect of dyeing paper on other materials like metal, Coptis chinensis have relatively stable qualities. Also, the three types of dyeed paper by Cotinus coggygria, Coptis chinensis and Phellodendri amurense, were found to have a tendency to remove harmful gases. Not only can these dyed papers be used for making storage box for cultural properties to prevent various organic artifacts from bio-damaging, but they can also be utilized as functional finishing materials in various spaces.

• Journal of Life Science
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• v.20 no.7
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• pp.1143-1150
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• 2010

The antibacterial activity of methanol extracts from 70 kinds of oriental medicinal herbs on four strains of Staphylococcus aureus and S. epidermidis was investigated. The results showed that C. japonica, C. sappan, R. javanica, R. tanguticum, and S. miltiorrhiza had an antibacterial activity on all the strains used. Among these, C. japonica and R. javanica, which showed excellent antibacterial activity, were extracted with water, ethanol, methanol, and ethyl acetate for further study of antibacterial activities. The results showed that the boiled water extract of C. japonica had the best antibacterial activity. Assuming that the antibacterial activity of C. japonica originated from berberine, which has been reported many times, the berberine content of the boiled water extract of C. japonica was analyzed and the result was 13.88%. To compare the antibacterial activity of berberine with that of other antibiotics, berberine chloride and three other kinds of antibiotics were investigated, which showed that berberine chloride had an antibacterial activity on KCCM 35494 S. epidermidis only when the concentration was higher than 600 mg/l, and it did not show antibacterial activity in the other strains. Based on these results, it was concluded that the antibacterial activity of the boiled water extract of C. japonica on the strains used in this study originated from high concentration of berberine or substances other than berberine. Therefore, identification of the substance will be necessary.

• Conservation Science in Museum
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• v.11
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• pp.61-72
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• 2010

This is the analysis of material dyed with Korean yellow dyes such as tumeric, amur cork tree, goldthread, gardenia, and the flowers of sophora japonica using nondestructive ultraviolet-visible and fluorescence spectrophotometry. In order to find out whether type of fabric or mordant influences analysis results, test fabrics were made using two types of fabric(silk and cotton) and dyed using three different mordants(no mordant, alum, iron). After analysis with UV-Vis reflectance spectrum on the dyed fabric, when the fabric was dyed with tumeric, amur cork tree and goldthread, the results were similar with no mordant and alum mordant, whereas there was a difference with an iron mordant. Also when the fabric was dyed using gardenia, different fabrics brought different results but there was no difference in results with mordants. On the other hand, when the fabric was dyed using the flowers of sophora japonica, there was no difference with fabrics but with mordants. After analysis with 3D-fluorescence spectrum, fabrics dyed with tumeric, amur cork tree and goldthread showed their own fluorescent spectrum with no regard to fabric and mordant; but with gardenia, there were differences with different fabrics whereas with the flowers of sophora japonica, there were differences with mordants.

• The Journal of Korean Medicine
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• v.29 no.4
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• pp.55-67
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• 2008

Objectives: The purpose of this study was to investigate the effect of Hwangryunhaedok-tang, its major component Coptidis Rhizoma, and the major component of Coptidis Rhizoma, berberine, on the expression of behavioral sensitization induced by the repeated administration of nicotine. Methods: Experimental ratswere divided into 3 groups and each group treated with saline, nicotine, and Hwangryunhaedok-tang. Experiments were performed on 4 further groups, each group treated with saline, nicotine, Coptidis Rhizoma, and berberine. To obtain rats addicted todrugs, nicotine was injected repeatedly. After 3 days of withdrawal phase, on the 11th day, rats were re-exposed to nicotine and thus able to induce the expression of the activity sensitization phenomenon successfully. To examine the change of c-Fos in the brain of experimental rats with the nicotine-induced activity sensitization, their neurological activation rate was assessed by c-Fos staining with Fos-like immunihistochemistry of the dopamine releasing area, the striatum and the nucleus accumbens. Results: The result demonstrate that Hwangryunhaedok-tang, its major component Coptidis Rhizoma and berberine,the major component of Coptidis Rhizoma, significantly reduced the walking activity rate in the drug addict models induced by nicotine administration, and Coptidis Rhizoma and its major component berberine reduce the marker of the neurological activation c-Fos, and thus revealed an effect of suppressing drug addiction. Conclusions: These results suggest the possibility of Hwangryunhaedok-tang, its major component Coptidis Rhizoma and the major component of Coptidis Rhizoma, berberine,as therapeutic for nicotine addiction.