• Title/Summary/Keyword: 환경유전자

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Identification and Molecular Characterization of Superoxide Dismutase Genes in Pseudomonas rhodesiae KK1 Capable of Polycyclic Aromatic Hydrocarbon Degradation (PAH를 분해할 수 있는 Pseudomonas rhodesiae KK1의 SOD 유전자의 동정 및 분자학적 특성 분석)

  • Lee, Dong-Heon;Oh, Kye-Heon;Kim, Seung Il;Kahng, Hyung-Yeel
    • Journal of Life Science
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    • v.26 no.1
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    • pp.75-82
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    • 2016
  • Pseudomonas rhodesiae KK1 has been reported to degrade polycyclic aromatic hydrocarbons (PAHs), such as anthracene, naphthalene, and phenanthrene, which are considered major environmental contaminants. Interestingly, antioxidant genes, including superoxide dismutase, are known to be expressed at different levels in response to environmental contaminants. This study was performed to identify the superoxide dismutase gene in strain KK1, which may be indirectly involved with degradation of PAHs, as well as to investigate the expression pattern of the superoxide dismutase gene in cells grown on different PAHs. Two types of superoxide dismutase genes responsible for the antioxidant defense mechanism, Mn-superoxide dismutase (sodA) and Fe-superoxide dismutase (sodB), were identified in P. rhodesiae KK1. The sodA gene in strain KK1 shared 95% similarity, based on 141 amino acids, with the Mn-sod of P. fluorescens Pf-5. The sodB strain, based on 135 amino acids, shared 99% similarity with the Fe-sod of P. fluorescens Pf-5. Southern hybridization using the sod gene fragment as a probe showed that at least two copies of superoxide dismutase genes exist in strain KK1. RT-PCR analysis revealed that the sodA and sodB genes were more strongly expressed in response to naphthalene and phenanthrene than to anthracene. Interestingly, sodA and sodB activities were revealed to be maintained in cells grown on all of the tested substrates, including glucose.

Performance Evaluation of a Genetic Algorithm-Based Multiuser Detector (유전자 알고리즘 기반 다중사용자 복조기의 성능 평가)

  • 김동호;정희창;김성철;이연우
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.7A
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    • pp.1227-1233
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    • 2001
  • 본 논문에서는 유전자 알고리즘을 기반으로 한 새로운 다중사용자 복조기를 제안하고, 최적(optimum) 다중사용자 복조기의 Hopfield 신경망 다중사용자 복조기를 비교 대상으로 하여 원근문제가 존재하는 환경에서 컴퓨터 시뮬레이션을 통해서 비트오율 성능을 비교하였다. 시뮬레이션 결과, 원근문제에 존재하는 채널환경에서는 본 논문에서 제안한 구조는 상당히 적은 계산량으로 최적의 다중사용자 복조기와 Hopfield 다중사용자 복조기와 근접한 성능을 기대할 수 있었고, 원근문제가 존재하지 않는 경우에서는 Hopfield 신경망구조보다 우월한 성능향상을 얻을 수 있었다.

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조피볼락 (Sebastes schlegeli) 뇌하수체 호르몬의 유전자 발현에 미치는 nonylphenol의 영향

  • 김대중;임상훈;민관식;정지현;한창희;전중균;손영창
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2003.05a
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    • pp.154-155
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    • 2003
  • 생식선자극호르몬 (GTHs; FSH/LH)은 어류의 생식계를 조절하는 상위의 호르몬 이며, 난황형성 및 생식세포의 분화/발달을 촉진하는 중추적인 기능을 담당한다. 그러나, 해양의 환경오염물질이 어류 GTH의 유전자발현에 미치는 영향에 관한 연구는 매우 부족한 실정이다 (Khan et al., 2001; Yadetie and Male, 2002). 본 연구에서는 난태생 어류인 조피볼락 뇌하수체 호르몬의 유전자 발현에 미치는 내분비계 장애물질의 영향에 관하여 조사하였다. (중략)

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Resposes of Two Cold - Regulated Genes, BN28 and BN115, in Field -Grown Canola (Brassica napus L.) (포장에서 케놀라 저온반응성 유전자 발현)

  • Moontae, Song
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.1
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    • pp.69-76
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    • 1995
  • Cold acclimation involves changes in gene expression. BN28 and BN115 are two genes which are regulated by cold temperature and assumed having roles in cold acclimation. The objectives of this experiment was to explore the expression of BN28 and BN115 under field conditions. Six winter cultivars were planted at three different dates during the fall. The expression of the genes was determined by northern blot analysis of total RNA taken from leaves 15 to 30 day-intervals after planting. The expression of the two genes was detected within 15 days after planting well before onset of freezing tolerance in plants. This suggestes either their expression was a prerequisite of the freezing tolerance or their expression was regulated by other environmental factors as well as temperature. Two genes showed a different expression pattern suggesting they had a different regulatory system. Although timecourse increase in expression of the cold-regulated genes was matched with increase in freezing tolerance, the difference of expression in cultivar level at specific times of measurement was not correlated with freezing tolerance at the moment.

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Major gene identification for LPL gene in Korean cattles (엘피엘 유전자에 대한 한우의 우수 유전자 조합 선별)

  • Jin, Mi-Hyun;Oh, Dong-Yep;Lee, Jea-Young
    • Journal of the Korean Data and Information Science Society
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    • v.24 no.6
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    • pp.1331-1339
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    • 2013
  • The lipoprotein lipase (LPL) gene can be considered a functional candidate gene that regulates fatty acid composition. Oh etc (2013) investigated the relationship between unsaturated fatty acids and five novel SNPs, and had confirmed that three polymorphic SNPs (c.322G>A, c.329A>T and c.1591G>A) were associated with fatty acid composition. We have used generalized linear model for adjusted environmental effects and multifactor dimensionality reduction (MDR) method to identify gene-gene interaction effect of statistical model in general. We applied the MDR method on the identify major interaction effects of exonic single nucleotide polymorphisms (SNPs) in the LPL gene for economic traits in Korean cattles population.

Distribution of Toxin Genes and Antimicrobial Resistance of Vibrio parahaemolyticus Isolated from Seafood in Gwangju (광주지역 유통·판매 수산물에서 분리된 장염비브리오의 독소유전자 분포 및 항생제 내성 조사)

  • Jeong, Hye Jin;Lee, Min Gyou;Lee, Hyang Hee;Seo, Si Eun;Jeong, So Hyang;Cho, Bae Sik;Seo, Jung Mi
    • Journal of Food Hygiene and Safety
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    • v.37 no.2
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    • pp.63-68
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    • 2022
  • The purpose of this study is to investigate the distribution of toxin genes and antimicrobial resistance of Vibrio parahaemolyticus isolated from seafood in Gwangju. A total of 335 seafood, including 163 shellfish, 97 fish, and 36 mollusk, were tested in this study. As a result, V. parahaemolyticus was detected in 123 (36.7%) of 335 seafood. The tdh gene was not detected in all strains, while the trh gene was detected in 3 strains (2.4%). According to antimicrobial susceptibility test, 116 strains (94.3%) represent resistance to ampicillin, and 1 strain (0.8%) represents resistance to trimethoprim/sulfametoxazole. However, all strains were sensitive to 9 antimicrobial agents, including amikacin, chloramphenicol, tetracycline, and more. Therefore, the risk of V. parahaemolyticus isolated from seafood in Gwangju is considered low, but continuous monitoring of V. parahaemolyticus in seafood is required.