• Journal of the Korean Society for Marine Environment & Energy
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• v.6 no.2
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• pp.16-27
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• 2003

For the last decade, some 400 small and large oil spill accidents have occurred every year. Such accident blow a serious damage to the marine resource and ecosystem, which can't be estimated in terms of economic and environmental losses. The physical/chemical methods used currently may be effective at the initial stage of accidents, but they can't serve to remove the spilled oil completely. Moreover, the dispersant may lead to a secondary contamination detrimental to the lives inhabiting wet lands, beaches and tidal zone. Thus, a new decomposing technology Is required for the environmentally sensitive areas. Bioremediation is the active use of biological techniques to mitigate the consequences of a spill using biological processes and refers both of stimulation of pollutant biodegradation and/or to enhance ecosystem recovery Bioremediation is an economically attractive method for the clean-up of oil-contaminated area. Bioremediation has been demonstrated to be an effective oil spill countermeasure for use in cobble, sand beach, salt marsh, and mud flat environment.

• Korean Journal of Ecology and Environment
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• v.36 no.2 s.103
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• pp.97-107
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• 2003

Fish vitellogenin produces in female liver during oogensesis through estradiol cycle, and produces even in male liver by endocrine-disrupting chemicals (EDCs) such as alkylphenols. The resulting effects of EDCs lead to the low fecundity of female and the feminization (eg. shrinkage of testis) in male. Especially, the production of vitellogenin in male indicates the environmental contamination of EDCs, resulting in the modulation of gene expression profiles and the monitoring of environmental contamination at specific area. In this paper, we suggest that fish vitellogenin is useful for biomonitoring for environmental contamination and would be substantially useful as a biomarker for a detection of EDCs in aquatic environment.

• The Journal of the Korea Contents Association
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• v.9 no.6
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• pp.1-8
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• 2009

There are a lot of influences, such as location of camera, luminosity, brightness, and direction of light, which affect the performance of 2-dimensional image recognition. This paper suggests an adaptive method for face-image recognition in noisy environments using evolvable filtering and feature extraction which uses one sample image from camera. This suggested method consists of two main parts. One is the environmental-adjustment module which determines optimum sets of filters, filter parameters, and dimensions of features by using "steady state genetic algorithm". The other another part is for face recognition module which performs recognition of face-image using the previous results. In the processing, we used Gabor wavelet for extracting features in the images and k-Nearest Neighbor method for the classification. For testing of the adaptive face recognition method, we tested the adaptive method in the brightness noise, in the impulse noise and in the composite noise and verified that the adaptive method protects face recognition-rate's rapidly decrease which can be occurred generally in the noisy environments.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.521-521
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• 2016

상수도 관망은 수원에서 수요절점까지 물을 안정적으로 공급하는 것을 목표로 한다. 상수도 관망의 최적설계는 수리학적 제한조건 (절점의 수압, 관로의 유속)을 만족하는 범위에서 비용을 최소화하는 설계안을 얻는 것으로 Savic and Walters (1997)는 유전 알고리즘 (Genetic Algorithms, Holland 1975)을 적용한 상수도 관망 설계 프로그램인 GANET를 제안하였고, Maier et al. (1996)은 개미군집알고리즘 (Ant Colony Optimization Algorithm, Dorigo et al. 1996)을 상수도 관망 최적설계에 적용한 후 그 결과가 유전 알고리즘에 비해 우수함을 증명하는 등 상수도 관망 최적설계에 관한 연구가 활발히 진행되어 오고 있다. 유전알고리즘은 선택, 교차, 돌연변이의 반복계산 과정을 통하여 최적해를 찾는 최적화 기법이다. 이 과정에서 결정변수는 유전자 (Gene)의 집합으로 표현되며, 염색체 (Chromosome) 내에서 근접한 유전 인자들은 일종의 Building Block을 형성하게 된다. Building Block은 좋은 해를 갖는 유전 인자를 높은 확률로 보관하여 지역해에 빠질 가능성을 줄이는 반면, 유전형 (Genotype)이 표현형 (Phenotype)을 충분히 모방하여 표현하지 못한 경우 오히려 최적해의 탐색을 방해할 수 있다는 한계점을 갖는다. 유전 알고리즘을 상수도 관망 최적설계에 적용하였을 때에도 이 한계점은 여실히 드러난다. 관로의 관경을 결정변수로 설정한 후 유전형으로 표현하였을 때, 관망도 상에서 근접하지 않은 두 관로가 염색체 내에서 연속으로 나열된다면 두 관로 간의 연관성이 실제보다 크게 고려되기 때문이다. 한편, 하모니써치 (Harmony Search, Geem et al. 2001) 알고리즘은 즉흥 연주 (Improvisation)를 통해 최상의 화음을 만들어내는 현상으로부터 착안하여 만들어진 최적화기법으로 연산 기법은 무작위선택, 기억회상, 피치조정 등으로 구성되어 있으며, 결정변수에 해당하는 연주자가 독립적으로 행동하며 해를 탐색한다는 점에서 유전알고리즘과 큰 차이를 갖는다. 본 연구에서는 유전알고리즘의 Building Block에 의해 발생하는 오류를 개선하고자, 상수도 관망 최적설계 연구에 많이 사용되는 Hanoi 관망 (Fujiwara and Khang 1990) 관로의 정렬 순서를 여러 가지 기준으로 설정하여 관망데이터를 구축한 후 하모니써치와 유전 알고리즘을 적용하여 최적화를 수행하였고 그 결과를 비교하였다. 그 결과 유전 알고리즘과 달리 하모니써치 알고리즘의 경우, 관로의 나열 순서와 상관없이 우수한 최적해 탐색 결과를 보이는 것을 확인할 수 있었다.

• Korean Journal of Veterinary Service
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• v.42 no.3
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• pp.153-159
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• 2019

The objective of this study was to identify mutations in the quinolone resistance determining region (QRDR) of the gyrA, gyrB, parC and parE genes, and the presence of plasmid-mediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrS, aac(6')-lb-cr and qepA in 40 nalidixic acid- resistant ($NA^R$) Salmonella isolates isolated from poultry slaughterhouse. The MIC of NA and ciprofloxacin for 40 $NA^R$ Salmonella isolates was $128{\sim}512{\mu}g/mL$ and < $0.125{\sim}0.25{\mu}g/mL$, respectively. The Salmonella isolates were resistant to NA (100%), gentamicin (5.0%) and ampicillin (2.5%). All $NA^R$ Salmonella isolates represented point mutation in codons Aspartic acid(Asp)-87 (90%) and Serine(Ser)-83 (10%) of QRDR of gyrA gene: $Asp87{\rightarrow}glycine$, $Ser83{\rightarrow}tyrosine$. No mutations were observed in QRDR of the gyrB, parC and parE gene. Moreover PMQR genes was not found in any of the tested isolates. Our findings showed that DNA gyrase is the primary target of quinolone resistance and a single mutation in codon Asp87 and Ser83 of the gyrA gene can confer resistance to NA and reduced susceptibility ciprofloxacin in Salmonella isolates.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.37-37
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• 2019

벼 재배포장에서 화학약제의 반복적인 사용으로 인한 병원균의 약제내성과 환경오염은 커다란 문제가 되고 있다. 이러한 약제내성을 극복하기 위한 노력의 하나로 유전자수준의 약제내성기작의 규명과 대체물질의 발굴이 필요하다고 사료된다. 이에 본 연구에서는 식물종이 지니는 광범위한 다양성에 주목하였고, 700여 종의 식물추출물을 벼 도열병균에 처리해 균사성장억제효과를 조사하는 선별실험을 실시하였고, 영릉향과 지모추출물이 높은 균사성장억제효과를 보이는 것을 확인했다. 영릉향과 지모 추출물이 화학약제를 대체하는 대안물질로서 가능성을 조사하기위해 균사성장억제능력 확인실험, 분획, HPLC (High Performance Liquid Chromatography), 포자발아 및 부착기 형성확인검정, ABC transporter의 발현조사, 환경독성실험등을 실시했다. 실험 결과, 영릉향과 지모 추출물을 벼 도열병균에 처리하였을 때 약제저항성유전자 ABC transporter의 기능이 저하되고 화학약제에 대한 내성이 감소를 확인했으며, 분획과 HPLC분석을 통해 영릉향과 지모의 유효성분을 확인했다. 또한, 실제 포장에서 영릉향 추출물과 지모 추출물을 사용하여 벼 도열병 방제가를 확인하는 포장시험을 실시한 결과, 각각 63%와 62%의 목 도열병 방제가를 확인 하였으며, 인축환경에 대한 유해성을 조사하기 위해 어류, 설치류, 중치류, 곤충을 대상으로 영릉향 추출물을 처리해 급성독성시험 실시한 결과, 고농도의 투여량에서도 독성이 없는 것을 확인했다. 위의 실험 결과를 토대로, 영릉향과 지모추출물이 화학농약에 의한 환경오염을 막을 수 있고, 인축과 환경에 피해를 입히지 않는 친환경자재로 친환경적인 생물농약의 신소재로서 가능성을 나타냈다고 사료된다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.6
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• pp.453-458
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• 2001

The Rural Development Administration (RDA) of Korea now operates a system called Rice Variety Selection Tests (RVST), which are now being implemented in eight Agricultural Research and Extension Services located in eight province RVST's objective is to provide accurate yield estimates and to select well-adapted varieties to each province. Systematic evaluation of entries included in RVST is a highly important task to select the best-adapted varieties to specific location and to observe the performance of entries across a wide range of test sites within a region. The rice yield data in RVST for ordinary transplanting in Kangwon province during 1997-2000 were analyzed. The experiments were carried out in three replications of a random complete block design with eleven entries across five locations. Additive Main effects and Multiplicative Interaction (AMMI) model was employed to examine the interaction between genotype and environment (G$\times$E) in the biplot form. It was found that genotype variability was as high as 66％, followed by G$\times$E interaction variability, 21％, and variability by environment, 13％. G$\times$E interaction was partitioned into two significant (P＜0.05) principal components. Pattern analysis was used for interpretation on G$\times$E interaction and adaptibility. Major determinants among the meteorological factors on G$\times$E matrix were canopy minimum temperature, minimum relative humidity, sunshine hours, precipitation and mean cloud amount. Odaebyeo, Obongbyeo and Jinbubyeo were relatively stable varieties in all the regions. Furthermore, the most adapted varieties in each region, in terms of productivity, were evaluated.

• Journal of Korean Society for Geospatial Information Science
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• v.8 no.1 s.15
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• pp.51-63
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• 2000

Dynamic traffic assignment(DTA) has been a topic of substantial research during the past decade. While DTA is gradually maturing, many aspects of DTA still need improvement, especially regarding its formulation and solution algerian Recently, with its promise for In(Intelligent Transportation System) and GIS(Geographic Information System) applications, DTA have received increasing attention. This potential also implies higher requirement for DTA modeling, especially regarding its solution efficiency for real-time implementation. But DTA have many mathematical difficulties in searching process due to the complexity of spatial and temporal variables. Although many solution algorithms have been studied, conventional methods cannot iud the solution in case that objective function or constraints is not convex. In this paper, the genetic algorithm to find the solution of DTA is applied and the Merchant-Nemhauser model is used as DTA model because it has a nonconvex constraint set. To handle the nonconvex constraint set the GENOCOP III system which is a kind of the genetic algorithm is used in this study. Results for the sample network have been compared with the results of conventional method.

• Korean Journal of Environmental Biology
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• v.40 no.4
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• pp.464-476
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• 2022

In recent years, macroalgal bloom occurs frequently in coastal oceans worldwide. It might be attributed to accelerating climate change. "Green tide" events caused by proliferation of green macroalgae (Ulva spp.) not only damage the local economy, but also harm coastal environments. These nuisance events have become common across several coastal regions of continents. In Korea, green tide incidences are readily seen throughout the year along the coastlines of Jeju Island, particularly the northeastern coast, since the 2000s. Ulva species are notorious to be difficult for morphology-based species identification due to their high degrees of phenotypic plasticity. In this study, to investigate temporal variation in Ulva community structure on Jeju Island between 2015 and 2020, chloroplast barcode tufA gene was sequenced and phylogenetically analyzed for 152 specimens from 24 sites. We found that Ulva ohnoi and Ulva pertusa known to be originated from subtropical regions were the most predominant all year round, suggesting that these two species contributed the most to local green tides in this region. While U. pertusa was relatively stable in frequency during 2015 to 2020, U. ohnoi increased 16% in frequency in 2020 (36.84%), which might be associated with rising sea surface temperature from which U. ohnoi could benefit. Two species (Ulva flexuosa, Ulva procera) of origins of Europe should be continuously monitored. The findings of this study provide valuable information and molecular genetic data of genus Ulva occurring in southern coasts of Korea, which will help mitigate negative influences of green tide events on Korea coast.

• Korean Journal of Microbiology
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• v.49 no.3
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• pp.237-244
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• 2013

Bacterial diversities in the guts of sea cucumbers (Apostichopus japonicus) and shrimps (Litopenaeus vannamei) were investigated using barcoded or tag-encoded 454 pyrosequencing of 16S rRNA genes. In sea cucumbers, most of sequences were related to two genera, the genus Propionigenium in the phylum Fusobacteria and an unclassified genus in the family Flavobacteriaceae of phylum Bacteroidetes. Shrimps showed various kinds of genera including Lactococcus, Leuconostoc, Prochlorococcus, and Vibrio as well as the unclassified genera in the families, Flavobacteriaceae, Rhodobacteraceae, Desulfobulbaceae, and Helicobacteraceae and in the order Mycoplasmatales. Unclassified genera containing environmental sequences only are more than half of genera from sea cucumbers and shrimps. Sea cucumbers and shrimps could be unexplored sources of novel microbes and the bacterial diversity of them was revealed by high throughput 454 pyrosequencing.