• Journal of Bio-Environment Control
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• v.15 no.1
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• pp.47-52
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• 2006

This studies were carried out in summer season to increase high temperature tolerance using hydrogen peroxide treatments on cucumber in greenhouse. The water stress of cucumber in greenhouse by the hydrogen peroxide treatments showed as control>250 mM>500 mM treatments in order. The photosynthesis rate of cucumber at $30^{\circ}C$ did not show difference with each hydrogen peroxide treatment in temperature controlled greenhouse. However, the photosynthesis rate of cucumber in the control and hydrogen peroxide treatments at $40^{\circ}C$ was significantly different. The photosynthesis rate of cucumber in combined treatment with 1,000 $mg{\cdot}L^{-1}\;CO_2$ supply and hydrogen peroxide was also higher than control, however, there was no different of photosynthesis in 250 mM and 500 mM treatment. The value of $F_v/F_m$ and $F_m/F_o$ of chlorophyll fluorescent in 500 mM hydrogen peroxide treatment at $40^{\circ}C$ was highest. Also the activity of POD, the antioxidant enzyme, was higher with high hydrogen peroxide concentration than the other treatments. The high temperature limits for growth were $43^{\circ}C$ in the control, $44^{\circ}C$ in the 250 mM and $46^{\circ}C$ in the 500 mM according to analyze chlorophyll fluorescent $F_o$. The high temperature tolerance in cucumber increased approximately $3^{\circ}C$ by the hydrogen peroxide treatments under this experiment conditions.

• Korean Journal of Environmental Biology
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• v.29 no.1
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• pp.68-73
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• 2011

The single cell gel electrophoresis (SCGE) assay is a microelectrophoretic technique for assessments of DNA damage at the level of the individual eukaryotic cell. The SCGE assay, due to its simplicity, sensitivity and need of a few cells, has advantages compared to other genomic damage assays such as sister chromatid exchange, chromosomal aberration and micronucleus test. In this study, investigated were the levels of DNA damage and the repair kinetics in the coelomocytes of Eisenia fetida treated with HgCl2 and ionizing radiation by means of the SCGE assay. For detecting DNA damage and repair in coelomocytes, earthworms (E. fetida) were irradiated with six doses of ${\gamma}$-rays (0, 2.5, 5, 10, 20 and 50 Gy) and in vivo exposed to mercuric chloride at 0, 80 and 160 mg $kg^{-1}$ for 48 hours. Then the Olive tail moments were measured during 0~12 hours after irradiation and 0~72 hours after Hg treatment. The results showed that the more the oxidative stress was induced by mercury and radiation, the longer the repair time was required. Also, the results suggest that the SCGE assay may be used as an important tool for comparison of the sensitivity of different species to oxidative stresses.

• Korean Journal of Environmental Biology
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• v.26 no.2
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• pp.94-101
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• 2008

The aim of this study was to assess the responses of mixed function oxygenase (MFO) and antioxidative systems of feral Javelin goby, Acanthogobius hasta, caught in two sites of different pollution level in Shihwa lake, which has been a highly polluted lake by organic pollutants from nearby industrial complexes and sites. Enzymes analyzed in phase I of MFO system are cytochrome P450 (CYP), NADPH-cytochrome P450 reductase (P450R), NADH-cytochrome b5 reductase (b5R), and ethoxyresorufin deethylase (EROD). Phase II enzyme of glutathione S-transferase (GST) in MFO system was also investigated. Moreover, oxidative-enzyme system including catalase (CAT), glutathione reductase (GR) and total-glutathione peroxidase (GPX) activities and glutathione concentration in both of oxidized (GSSG) and reduced form (GSH) were determined. P450R, b5R, and GST activities of fish are relatively high in the polluted area, whereas hepatic EROD activity levels of fish in polluted area were lower than those of unpolluted area. CYP concentrations are not different between areas. These results indicated that feral Acanthogobius hasta were adaptive to highly polluted environment and exposed to oxidative stress in Shihwa lake.

• Journal of Life Science
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• v.20 no.1
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• pp.22-26
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• 2010

Seven rice germplasms with red pigmentation within the pericarp were isolated from a large mutant collection. These red pericarp phenotypes resulted from the functions of the Rc, Rd and RdRc genes. Among them, two brown pericarp of the Rc type, four red pericarp of the RdRc type, and one white pericarp of the Rd type were identified. Morphological and agronomic characteristics of those rice germplasms were studied. The Rc type germplasms have the faint red or brown color pericarp and the Rd types produce the white pericarp, whereas the RdRc type germplasms have the dark red pericarp. Most of the important agronomic characteristics including plant stature, tillering ability, spikelet fertility, and total grain yield were lower in the colored rice than those of the wild-type control. All of the studied colored rice germplasms had a tendency of easy seed-shattering in comparison to the control. These characteristics of newly identified germplasms will be useful for identifying the genes responsible for pericarp color phenotype determination.

• Journal of Life Science
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• v.27 no.5
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• pp.591-599
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• 2017

It is well established that endurance performance is negatively affected by environmental heat stress. Numerous scientific investigations have attempted to improve performance in the heat with pre-cooling and per-cooling for endurance athletes. Some cooling strategies are more logistically challenging than others, and thus are often impractical for use in training or competition. The purpose of this study was to review the literature on the use of cooling interventions in the improvement of performance and recovery from exercise-induced heat stress. We undertook an examination that focused on the effects of pre-cooling and per-cooling on the improvement in endurance performance and the effects of post-exercise cooling on recovery. The benefits for pre-cooling and per-cooling strategies undertaken in the laboratory setting could be employed by athletes who compete in hot environmental conditions to improve performance. Most laboratory studies have shown improvements in endurance performance following pre-cooling and per-cooling, and in recovery following post-cooling. Cooling strategies such as cooling vest, neck cooling collar, menthol and ice slurry are practically relevant to sports field. Cooling interventions that can be applied frequently to reduce thermal strain prior to, during and directly after training appear to be the best effective strategy to improve performance and recovery. Future research is warranted to investigate the effectiveness of practical pre-cooling and per-cooling strategies in competition or field settings.

• Journal of the Korean Society of Marine Environment & Safety
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• v.16 no.2
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• pp.201-207
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• 2010

Recently, about 600 cases of marine accidents occur annually in Korea. According to many studies and analyses on occurrence of marine accidents, 70~80% of marine accidents were caused by human factors. Among the human factors, navigation officers' fatigue is very important factor. Although navigation officers' fatigue serves as an important role in marine accidents, there is no method to exactly examine the degree of officers' fatigue. Accordingly, this study analyzed human factors according to types of marine accidents and extracted important five factors affecting navigation officers' fatigue through the questionnaire survey by means of literatures and 5-point scale. In addition, evaluation factors of marine accident risks caused by fatigue factors were divided and structured by using ISM. Lastly, it found out the importance of each fatigue factor drawn by AHP and decided marine accidents that were most highly caused by navigation officers' fatigue in order. At the result, weights were high as sleep time 0.385, stress 0.302, health condition 0.139, rest time 0.099, alcohol and drug 0.074 in fatigue factors, and death and injury 0.328, collision 0.308, grounding 0.195, sinking 0.094, fire accident 0.075 in evaluation factors of marine accident risks. Therefore, the control plan to lower marine accident risks should be prepared on the basis of high weight factors.

• Journal of Life Science
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• v.23 no.10
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• pp.1223-1229
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• 2013

Ursolic acid (UA) a bio-active ingredient found in a variety of fruits and vegetables, and it has potent antioxidant activity. However, the role of UA in mouse embryonic stem (ES) cells is poorly understood. This study investigated the functional role of UA in regulating the development of mouse ES cells under hypoxia. Hypoxia did not exert a significant effect on the undifferentiated state of mouse ES cells. However, it induced reactive oxygen species (ROS) generation and increased the level of lactate dehydrogenase (LDH) production at 48 h of hypoxic exposure. Conversely, oxidative stress induced by hypoxia was significantly inhibited by UA ($30{\mu}M$) pretreatment. Hypoxia significantly decreased cell survival and the level of [$^3H$] thymidine incorporation, both of which recovered following pretreatment of UA. In addition, UA decreased the apoptotic effect of hypoxia by attenuating caspase-3 cleavage or by recovering cellular inhibition of the apoptotic protein (cIAP)-2 and Bcl-2 expression. We further found that UA decreased senescence-associated beta-galactosidase activity. We suggest that UA is a natural antioxidant and one of the functional modulators of hypoxia-induced survival, apoptosis, proliferation, and aging in mouse ES cells.

• Proceedings of The Korean Society of Health Promotion Conference
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• 2005.09a
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• pp.55-88
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• 2005

Healthy Japan 21의 목적은 삶의 질을 향상시킴은 물론 노동가능 인구집단의 유병률을 줄이고 건강수명을 연장시키기 위하여, 21세기 모든 일본인들이 더욱 건강하고 행복한 삶을 향유할 수 있도록 국가사업을 활성화하는 것이다. 구체적 목적은 조기사망을 예방하고 건강생활을 향상하는 것으로, Healthy Japan 21의 전략적 기획과정에서 건강수명의 연장을 실현하기 위하여 2010년까지 달성되어야 하는 구체적인 목적들이 또한 제시되었다. 조기사망을 감소시키기 위하여 사고, 암, 자살, 심장병 감소의 중요성을 인식하고 9개의 주요 목표로 영양, 신체활동, 휴식과 이완, 금연, 절주, 구강보건, 당뇨병 예방, 심혈관계질환 예방, 암 예방을 설정하였다. 흡연, 알콜, 식사 그리고 운동과 같은 생활양식은 스트레스, 비만, 고혈압과 같은 위험요인 및 질병관리와 관련이 있으며, 위험요인은 암, 심장병, 구강질환의 발생과 밀접한 관련을 갖는다. 따라서 질병의 발생을 예방하기 위해서는 건강증진과 일차예방이 강조되어야 한다. 일차예방에 중점을 두기 위해서 우리는 전통적인 질병관리의 중점사항인 정기적인 건강검진을 통해 질병 조기발견을 노력을 게을리 하지 말아야 할 것이다. 아울러 의료비 감소, 병상에 있는 사람들의 감소, 사회세의 감소도 또한 달성되어야 하는 사업의 결과로 설정되어 있다. 가장 최근의 Healthy Japan 2000(1998-99)의 평가에 따르면 목표들의 15%가 달성되었거나 초과 달성된 것으로 나타났다. 이 중 아동과 청소년의 사망률의 경우, 1-14세 아동의 사망률은 1987년부터 26%가 감소되어 2000년도 목표인 인구 100,000명단 28명의 사망을 초과 달성한 것으로 평가되었다. Healthy Japan 21의 두 가지 주요 전략은 일반 인구집단을 위한 전략과 고위험 집단을 대상으로 하는 전략으로 구성된다. 개인의 건강한 생활양식으로의 변화를 포함한 우리의 건강증진 노력은 사람 중심으로 개인의 선택을 기반으로 하고 있다. 이러한 노력을 지원하기 위하여, 각 개인이 정보를 갖은 상태에서 올바른 선택을 할 수 있도록 적당량의 올바른 정보를 제공하는 것이 필수적이다. 이와 같은 일본의 건강증진계획은 2000년 3월에 Healthy Japan 21이 설립되었으며, 2000-2002년 사이 모든 현이 자신의 사업계획을 설정하였으며, 2001-2005년에는 약 반수 정도의 지방자치단체들이 자신들의 사업계획을 확정하였다. 건강증진을 이루는 중요한 수단은 파트너 쉽에 있다. 정부조직 뿐 아니라 건강보험회사, 보건의료서비스 제공자, 교육단체, 대중매체, 사기업, 봉사단체 등을 포함한 건강분야의 조직들은 자신들의 전문적 기술들을 한데 모아 서로 협력하여야 한다. 또 하나의 중요한 수단은 건강 지지적인 환경이다. 개인의 건강증진 노력을 체계화함으로써 지지적인 환경을 조성할 수 있다. Healthy Japan 21에 대한 평가는 2005년에 중간평가가, 2010에 최종평가가 있을 예정이다. 평가결과들은 이후에 진행될 사업의 향상을 위한 기준으로 활용될 예정이다.

• Journal of Environmental Health Sciences
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• v.43 no.5
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• pp.370-381
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• 2017

Objectives: The rate at which the nationwide highway is outsourcing the duties of toll gate cashiers to the public is increasing. The aim of this study is to conduct research on job stress for the rate at which the fares are collected by national highway toll gate cashiers. Methods: The population selected was a statistically significant group making up 10% of the total number of people surveyed in 2015, with a population of approximately 5,495 persons. The sample size is intended to cover the maximum sample size of 550 samples and the maximum number of samples that should be obtained. After allocating the number of samples by quota allocation, the qualitative function of Quality Function Deployment (QFD) was applied by random sampling. Results: Job stress ranked at $46.6{\pm}11.5$ points was found to be under the stress of 50% reported in other occupations. Job stress items showed higher for "job instability" compared to other types of tasks. Also, it appeared lower in "conflict in relationships." This is a result of the work characteristics of highway service operators. Conclusions: Time was found to have the highest influence on the workload (${\beta}=0.80$, p. 0.001), which calls for a reduction in time and workload. est influence on the workload (${\beta}=0.80$, p. 0.001), which requires a reduction in time and workload.

• Korean journal of applied entomology
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• v.39 no.1
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• pp.31-41
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• 2000

Community analysis of oribatid mites was conducted in Namsan and Kwangreung coniferous forests which have been received by different degrees of environmental pressures through urbanization processes. Oribatid mites were sampled in the litter and soil layer of study sites from May 1993 to October 1994. Although two sites have been under similar weather condition, seasonal changes in oribatid mites density did not show a synchronized pattern. Density in spring and summer showed stable pattern with low fluctuations, but unstable pattern in autumn between 1993 and 1994. And these patterns were highly correlated with precipitation. The density and species number were higher in the litter layer than in the soil layer and showed no typical seasonal changes. The dominant species were Scheloribates latipes (1 l.78%), Pergalumna altera (8.92%), Eohypochthonius crassisetiger (7.58%), Scheloribates sp. (6.89%) and Suctobelbella yezoensis (5.04%) in Namsan, and Ceratozetes japonicus (25.72%), Punctoribates punctum (14.15%), Trichogalumna nipponica (10.96%) and Ramusella sengbuschi (5.08%) in Kwangreung. The number of species with high constany were 10 and 18 in Namsan and Kwangreung, respectively. Namsan showed the feature of urban forests. In analysis of species diversity, species richness was significantly higher in Kwangreung than in Namsan, while shannon (H') and evenness index (J') were higher in Namsan than in Kwangreung. The values of shannon index (H') in Namsan and Kwangreugn were 2.83 and 2.62, respectively and evenness index (J') were 0.78 and 0.67, respectively. The value of similarity index between two sites was 0.68.