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Responses in Hepatic Xenobiotic Metabolizing and Antioxidant Enzymes in Javelin Goby Acanthogobius hasta Collected at Shihwa Lake  

Lee, Ji-Seon (Kangnung National University.EMBRC)
Jeong, Jee-Hyun (Dong-eui University)
Han, Chang-Hee (KORDI South Sea Institute)
Shim, Won-Joon (Dong-eui University)
Jeon, Joong-Kyun (Kangnung National University.EMBRC)
Publication Information
Korean Journal of Environmental Biology / v.26, no.2, 2008 , pp. 94-101 More about this Journal
Abstract
The aim of this study was to assess the responses of mixed function oxygenase (MFO) and antioxidative systems of feral Javelin goby, Acanthogobius hasta, caught in two sites of different pollution level in Shihwa lake, which has been a highly polluted lake by organic pollutants from nearby industrial complexes and sites. Enzymes analyzed in phase I of MFO system are cytochrome P450 (CYP), NADPH-cytochrome P450 reductase (P450R), NADH-cytochrome b5 reductase (b5R), and ethoxyresorufin deethylase (EROD). Phase II enzyme of glutathione S-transferase (GST) in MFO system was also investigated. Moreover, oxidative-enzyme system including catalase (CAT), glutathione reductase (GR) and total-glutathione peroxidase (GPX) activities and glutathione concentration in both of oxidized (GSSG) and reduced form (GSH) were determined. P450R, b5R, and GST activities of fish are relatively high in the polluted area, whereas hepatic EROD activity levels of fish in polluted area were lower than those of unpolluted area. CYP concentrations are not different between areas. These results indicated that feral Acanthogobius hasta were adaptive to highly polluted environment and exposed to oxidative stress in Shihwa lake.
Keywords
xenobiotic metabolizing enzyme; antioxidative system; Javelin goby; Acanthogobius hasta; Shihwa lake;
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