• Journal of radiological science and technology
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• v.29 no.2
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• pp.83-92
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• 2006

Based on the data of cervical cancer patients who were treated by the radiotherapy with concurrent chemotherapy at the radiation oncology department of National Cancer Center from January 2002 to February 2003, we have studied the method recommended by ICRU 38 to maximize the prescription dose to the planing target volume (PTV) with minimizing the dose to surrounding normal organs. Clinical stage of the patients are 1 patient for stage IB, 3 patients for IIA, 19 patients for IIB, 3 patients for IIIA, 3 patients for IIIB and 1 patient for IV. All patients took the MRI before treatment and the maximum size of the gross tumor volume were under 4cm for 17 patients and from 4 cm to 6 cm for 12 patients and above 6 cm for 1 patient. The results show that while the irradiated volume can be reduced with optimized dose distribution using PTV treatment planning (p<0.0001) when the remained tumor size is small, the surrounding normal organs will receive unnecessarily large dose when the irradiated tumor volume is relatively large. This is because there is some limitation in controling the intensity of radiation in Fletcher Williamson Applicator. To overcome the limit of applicator and to achieve the optimal dose distribution, we have virtually applied 4 needles with Fletcher Williamson Applicator for 10 patients who have relatively large tumor and studied the change in dose distribution before and after application. The results show that this new virtual treatment plan reduces the volume covered by 100 % isodose (p=0.0608, p=0.0607) and reduces the dose of normal organs (p=0.0162, p=0.008). This evidence suggest that this method is superior than the currently used method such as PTV treatment and ICRU treatment.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.1
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• pp.3-15
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• 1997

Iron oxide compounds in 8 selected Cheju Island soil samples have been analized by X-ray fluorescence spectrometer(XRF), X-ray diffractometry(XRD), selected chemical techniques, and $M{\ddot{o}}ssbauer$ spectroscopy. The result of this analysis by XRF shows that the rate of quantity of $Fe_2O_3$ in 8 soil samples was from 8.03wt.%(Daejeong paddy soil) to 18.21wt.%(Songag soils). Songag, Heugag and Gueom soils were detected to have lower peaks of intensity of hematite by XRD. In addition, these soils were not detected to have hematite and goethite peaks. Ferrihydrite, which is a short-range-order mineral commonly present in volcanic ash soil, was not detected by XRD due to low concentration and/or poor cristallinity. Ferrihydrite contents estimated from Feo values were 8.8~35.2g/kg for volcanic ash soils and 0.85g/kg for the Daejeong soil. Most of the soil samples represented by the paramagnetic $Fe^{3+}$ doublet obtained from $M{\ddot{o}}ssbauer$ spectra at room temperature and 18K were considered to arise from the presence of ferrihydrite, superparamagnetic goethite, and silicate minerals. Also the paramagnetic $Fe^{2+}$ doublets are attributable to primary minerals such as olivine, illite, chlorite, augite, biotite, and hornblende. Goethite and hematite were identified as the dominant crystalline iron oxides in these soils from $M{\ddot{o}}ssbauer$ spectra obtained at room temperature and 18K. All the soil samples exhibited strong superparamagnetic relaxation. Collapse of the $M{\ddot{o}}ssbauer$ magnetic hyperfine splitting at room temperature was due to the small size(${\sim}180{\AA}$) of the oxide particles and/or Al-subsituted goethite.

• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.257-261
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• 1998

The repeated subcultures of in vitro plant materials in wasabi became highly vitrified and the capacity for multiple shoot formation from the vitrified plant materials was very low. In order to improve the quality of in vitro propagated planting materials, the experiments were carried out using culture vessels capped with membrane filter(MF). When vitrified shoots were cultured on MS medium with 0.2mg/L BA in the vessels with MF or without MF for 60 days, the shoots in the vessels with MF did not vitrified. In contrast, the shoots grown in the vessels without MF vitrified at 65%. The stomates of vitrified leaves were circular and inflated, whereas those of normal leaves acclimatizated in the vessels with MF were ovate in shape. The hardened shoots were also cultured on MS media without sucrose containing 0.01mg/L IBA in vessels with(photoautotrophic culture) or without(control) MF. Sucrose was necessary for survival of the in vitro plantlets in the vessels without MF. After 20 days of culture, the shoots in the vessels without MF on the sucrose-free media turned yellow and died. But the shoots in the vessels with MF in the sucrose-free media produced a lot of roots. When shoots were cultured on MS medium with 2% sucrose containing 0.01mg/L IBA in the vessels with(photomixotrophic culture) or without(heterotrophic culture) MF, best growth occured in photomixotrophic culture.

• Korean Journal of Food Science and Technology
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• v.50 no.1
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• pp.117-121
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• 2018

Puffing process modifies the chemical and physical properties of the grains. In this study, oats were puffed by subjecting them to pressure of 1.0 and 1.2 MPa, following which the bioactive constituents and antioxidant activities in the oat extracts were investigated. The polyphenol content in puffed oat extracts increased in a pressure-dependent manner (109 and 157 mg gallic acid equivalent/100 g at 1.0 and 1.2 MPa, respectively). In addition, gallic acid was synthesized after puffing ($518{\mu}g/g$ of extract at 1.0 MPa) and was the most abundant phenolic acid in puffed oats. The antioxidant activities, which were determined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activities, were improved in oat extracts after puffing (+245 and +184% at 1.2 MPa, respectively). In conclusion, puffing process of oats increased the extractability of polyphenols, including gallic acid, which positively affected its antioxidant activities. These results will provide useful information when using puffed oats for food production.

• Current Research on Agriculture and Life Sciences
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• v.5
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• pp.185-192
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• 1987

Anethole, eugenol, isoeugenol, safrole and isosafrole are ingredients of refined oils which are obtained from some plants and their chemical structures are very similar. They are mainly used as a flavoring agent, food additive, carminative. dental analgesics and for many drugs. But, there is no report about their effect on gastric secretion and gastric motility. To examine the effect of anethole, eugenol, isoeugenol, safrole and isosafrole on gastric secretion in rats and gastric motility in pigeons, this paper was investigated. The results were as follows ; 1. All of 5 essential oils showed significant inhibitory effect compared with control group on gastric secretion at the rate of 1.00 ml/100 g, 0.50 ml/100 g B. W. in the rat. 2. Eugenol, isoeugenol and isosafrole showed significant inhibitory effect on gastric secretion at the rate of 0.25 ml/100 g B. W. in the rat. 3. Isosafrole showed the most inhibitory effect on gastric secretion in 5 essential oils. 4. All of 5 essential oils, in a more or less degree. showed temporary inhibitory effect on gastric motility in the pigeon. 5. In conclusion, all of 5 essential oils showed inhibitory action on gastric secretion in the rat and gastric motility in the pigeon.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.4 s.54
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• pp.349-357
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• 2005

New antioxidative substances for cosmeceuticals were screened from natural resources such as microbial metabolites, mushrooms, and medicinal plants. Four antioxidants were isolated from the fungal metabolite of Eupenicillium shearii and their structures were determined to be new phenolic compounds. The compounds were designated as melanocins A, B, C, and D. Melanocins $A{\sim}D$ exhibited free radical scavenging activity on DPPH and superoxide with $EC_{50}$ values of $21{\sim}94\;and\;7{\sim}84{\mu}M$, respectively, which were stronger activity than those of ${\alpha}-tocopherol$ and BHA. Melanocin A showed anti-wrinkle effects on the UV-irrated hairless mouse skin. A novel hispidin antioxidative compound designated as inoscavin A was isolated from the fruiting body of the mushroom, Inonotus xeranticus. Inoscavin A scavenged superoxide radical with $EC_{50}$ values of $0.03{\mu}g/mL$, and inhibited rat liver microsomal lipid peroxidation with $EC_{50}$ values of $0.3{\mu}g/mL$. Benzastatins $A{\sim}G$, the novel antioxidants isolated from the culture of Streptomyces nitrosporeus showed potent lipid peroxidation inhibitory activity with $EC_{50}$ values of $3{\sim}30{\mu}M$. A cyclopentene compound with strong hypopigmentary effect was isolated from the fungal metabolite of Penicillium sp. and identifed as terrein. Terrein significantly reduced melanin levels in a melanomacyte cell line, Mel-Ab. It showed 10 times stronger activity than kojic acid, but exhibited no cytotoxic effect even in $100{\mu}M$. It was suggested that terrein reduced melanin synthesis by reducing tyrosinase production by MITF down-regulation.

• Korean Journal of Environmental Biology
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• v.37 no.1
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• pp.93-108
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• 2019

To investigate the temporal-spatial distribution of picophytoplankton in relation to different water masses in the northern East China Sea (ECS), picophytoplankton abundance were investigated using flow cytometry with environmental factors in 2016-2017. The results from the analysis of flow cytometer data showed that Synechococcus appeared across all seasons, exhibiting its minimum abundance in winter and maximum abundance in summer. Furthermore, high abundance was detected in the surface mixed layer during spring and summer when vertical stratification occurs; in particular, Synechococcus exhibited maximum abundance in thermocline layer, indicating a close correlation to water temperature and thermocline formation. In addition, the abundance of Synechococcus indicated a decrease in the western seas in 2017 compared to 2016 under the strong influence of the Changjiang Diluted Water (CDW). This was determined by the significant influence of the CDW on the abundance of Synechococcus during summer in the northern waters of the ECS. In contrast, Prochlorococcus did not appear during winter and spring, and its distribution was limited during summer and autumn in the eastern seas under the influence of the Kuroshio current. The largest range of Prochlorococcus distribution was confirmed during autumn without the influence of the CDW. Thus, the distribution pattern of each picophytoplankton genus was found to be changing in accordance to the extension and reduction of sea current in different seasons and periods of time. This is anticipated to be a useful biological marker in understanding the distribution of sea currents and their influence in the northern waters of the ECS.

• Korean Journal of Environmental Biology
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• v.37 no.1
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• pp.19-30
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• 2019

To investigate the characteristics of seasonal environment and phytoplankton community structure in the coastal area of Dokdo, a survey of Dokdo around waters was conducted during the four seasons. Phytoplankton of 4 phylum 72 species in four seasons were collected in Dokdo around water. The seasonal mean abundance of phytoplankton were $3.32{\times}10^4cells\;L^{-1}$ in winter, $1.04{\times}10^4cells\;L^{-1}$ in spring, $0.28{\times}10^4cells\;L^{-1}$ in summer, and $4.86{\times}10^4cells\;L^{-1}$ in autumn in Dokdo around water. During winter, the diatoms Chaetoceros spp. had dominated. During spring, when the nutrients in the euphotic layer were depleted, the nano-flagellates and Cryptomonas appeared at surface layer. In summer, the abundance of phytoplankton was relatively low, which lead to occurrence of diatoms such as genus of Chaetoceros, Rhizosolenia, and Skeletonema. In autumn, Pseudo-nitzschia spp. was the most dominant species and tropical species such as Amphisolenia sp. and Ornithocercus magnificus were observed, implying that they may have introduced within warm water current such as Kurosiwo Current. Therefore, although natural phytoplankton communities in the vicinity water of Dokdo are mainly influenced by Tsushima Warm Current branched Kurosiwo Current, their population dynamics was affected on the spatio-temporal change of physicochemical factors by short-term wind events, namely "island effect". Long-term survey research is needed to facilitate food-web response in marine ecosystem associated with phytoplankton biomass and physicochemical factors including the warm water current in oligotrophic offshore water of Dokdo, which may have significant role for sustainable use of Dokdo.

• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.236-245
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• 2019

Fruit ripening is a genetically programmed process involving a number of biochemical and physiological processes assisted by variations in gene expression and enzyme activities. This process generally affects the phytochemical profile and the bioactive principles in fruits and vegetables. To appraise the variation in bioactive principles of fruits from Rosa rugosa during its ripening process, we analyzed the changes in antioxidant and anti-elastase activities and polyphenolic compounds during the four ripening stages of fruits. Overall, an extract of unripe fruits contained the highest levels of total phenolic and flavonoid contents, radical scavenging activity, reducing power, oxygen radical antioxidant capacity, and elastase inhibitory activity, compared with the extracts of fruits at other stages of ripening. Additionally, we found that the reduction of flavonoid content occurs because of decreased transcriptional levels of genes involved in flavonoid biosynthesis pathway during the ripening process. Based on HPLC analysis, we found that the extract of unripe fruits contained the highest amount of myricetin, caffeic acid, chlorogenic acid, syringic acid, and p-coumaric acid and suggested that the antioxidant and anti-elastase activities of the extract obtained from stage 1, should be mediated by the presence of these compounds. Additionally, we analyzed the interaction sites and patterns between these compounds and elastase using the structure-based molecular docking approach, and suggested that chlorogenic acid strongly interacted with elastase. Together, these findings suggest that the maturity of fruits has profound effects on the pharmaceutical value of R. rugosa.

• Korean Journal of Environmental Biology
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• v.38 no.4
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• pp.658-666
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• 2020

The purpose of this study was to select a suitable planting substrate for multilayered plantings in an apartment landscape space. The experiment was conducted between May to October 2019, at the National Institute of Horticultural and Herbal Science. Planting substrate was prepared in six repetitions of eight treatment zones using mulching material, horticultural soil, bottom ash, and subgrade soil. Rosa hybrid 'Barkarole' and Hydrangea macrophylla 'Nikko Blue' were selected as the experimental plants. We investigated the monthly variation and effect of the substrate type on the growth (plant height, number of branches, leaf length, leaf width, and plant area of the substrates) of the plants. In R. hybrid 'Barkarole' grown in 20 cm of horticultural soil and 10 cm of bottom ash, the plants were taller(102.2±5.8 cm), had more branches (5.5±0.6 each), longer leaves (10.9±1.0 cm), and greater leaf width (6.2±0.5 cm) and plant area (4077.1±416.6 cm²)(p<0.05). H. macrophylla 'Nikko Blue' showed the best growth from 3cm of mulching, 20cm of horticultural topsoil, and 10cm of bottom ash, which resulted in taller plants (43.6±2.1 cm), more branches (4.9±0.8 each), longer leaves (7.2±0.5 cm), and greater leaf width(4.3±0.3 cm) and plant area (344.5±43.2 cm²). Through this study, it was possible to propose an optimal planting substrate for shrubs for multi-layered landscaping.