• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.193-200
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• 2007

Pollen grains of seven taxa on the family Pyrolaceae, known to be distributed in Korea (Pyrola dahurica(Andreses) Kom., P. incarnata (DC.) Fisch. ex Kom., P. japonica Klenze ex Alefeld, P. minor L., P. renifolia Max., Orthilia secunda (L.) House, Chimaphila japonica Miq.), were examined by the light and the scanning electron microscope in order to evaluate their taxonomic significances. The pollen units of genus Pyrola, Orthilia, Chimaphila were tetrad, monad, polyad respectively. This characteristics was good character for delimiting the genus, and Orthilia secunda (L.) House was more proper scientific name than Pyrola secunda L. based upon this character. Pollen size, tricolporate length, surface sculpturing pattern were different slightly among the treated taxa, and surface sculpturing patterns of Pyrola dahurica, P. incarnate, P. renifolia, Chimaphiza japonica were different from previous reports. Evolutionary trends of pollen grains in Pyrolaceae were inferred from the pollen units and aperture shapes.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.284-290
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• 2010

This study was carried out to breed a self-compatible variety of Tiger Lily from the seedlings originated from Chinju city, Gyeongsangnam-do. The morphological characteristics, ploidy, and the resistance of seedlings to virus infection were investigated. A progeny test was also conducted to examine whether the propagated progenies had the same characteristics as the mother plant. The self-compatible diploid lily variety developed in this study showed a tall type like native triploid lily, Lilium tigrinum, and bulbils were formed on both lily varieties. The morphological characteristics of the flowers and leaves were not much different between the varieties, while the size of the bulbils and bulbs showed significant differences. The percentage of seed generation by self-pollination was 72.6% for the self-compatible variety, while there was no seed generated for native triploid lily. The number of chromosome was 2n = 26 (x = 13, diploid) for the self-compatible variety, while 2n = 39 (x = 13, triploid) in native lily variety. The progenies of the self-compatible diploid lily variety showed the same characteristics as those of its mother plant in morphology, seed germination, and polyploidy. The mother plant of the self-compatible diploid lily variety showed 58% pollen germination and the 2-year-old and 3-year-old progenies showed similar germination percentages. The pollen grains of Korean native triploid lily, however, never germinated.

• Journal of Food Hygiene and Safety
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• v.38 no.4
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• pp.202-210
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• 2023

To analyze the pesticide residues in commercial bee pollen products in South Korea, 61 samples were collected and screened for 339 pesticides. Results revealed that approximately 34% (>LOQ) of samples were contaminated with at least one pesticide. The pesticide residue detection rates of domestic and imported samples were 31% and 44%, respectively. Furthermore, the pesticide residue detection rate of online distribution (60%) was higher than that of offline distribution (27%). Fifteen pesticides were discovered in bee pollen, and pendimethalin, chlorfenvinphos, chlorpyrifos, and fluazinam were detected in 7, 6, 3, and 2 order of frequency, respectively. Even though its concentration was low, chlorfenvinphos which is banned in food crops in the United States, European Union, and Korea, was detected in bee pollen samples commonly. Therefore, continuous investigation of pesticide residues in bee pollen products and their acceptance criteria is required for safety.

• Applied Microscopy
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• v.32 no.4
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• pp.345-360
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• 2002

Pollen morphology of four genera, 22 species of Korean Crassulaceae has been examined with light and scanning electron microscopy. The presence/absence of the aperture margo and granules was useful characters to clarify the generic boundaries, and the shapes of the streate were significant to delineate taxonomic relationships within the genus Sedum. The pollen grains of Korean Crassulaceae were small and medium in size. Hylotelephium viviparum has the smallest pollen grains, and H. viridescens the largest. The pollen grains are tricolporate, circular in polar view and were prolate to prolate-spheroidal in equatorial shape. Korean Crassulaceae species have striate grains. The close pollen morphological relationships among Hylotelephium, Meterostachys and Orostachys species were concordant to the results based on the previous molecular and morphological studies. The results of the clustering analysis using pollen characters support the segregation of Sedum sensu lato which has been proposed by the previous authors. Within the genus Sedum, S. kamtschatium is closely related to S. middendorffianum with granules on the surface of the pollen grains. Sedum polytrichoides, a member of the Sedum, was clustered to the species of the Meterostachys, Hylotelephium and Orostachys, and needs to reexamine the taxonomic status. O. malacophyllus collected at Chungdong-Jin was significantly differ from the same species from Gampo in pollen and morphological characters.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 1994.07a
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• pp.23-24
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• 1994

본 연구는 성숙과의 저장중에 세포벽분해효소가 세포벽을 분해해서 물성의 변화를 유발함으로 일어나는 과실의 연과가 품질과 저장성의 저하 뿐만 아니라 영양적, 경제적 손실을 초래한다는 점을 고려하여 대추의 성숙중에 일어나는 연화현상을 연구하고자 성숙에 따른 경도, 세포벽분해효소의 활성, 세포벽 다당류 pectin질, 비섬유성 중성당 및 조직의 변화를 조사하였다. 경도는 대추의 숙성에 따라 감소하였고, polygalacturonase와 $\beta$-galactosidase의 활성은 각각 변색기와 완숙기에 나타난 이후 급격히 증가하였다. 세포벽 다당류인 pectin질과 알칼리 지용성 hemicellulose는 완숙기가지 증가햐였으나 cellulose는 완숙기에 산가용성 hemicellulose와 cellulose를 제외한 세포벽 다당류의 함량은 다소 감소하였다. 대추의 세포벽 비섬유성 중성당으로 rhamnose, arabinose, xylose, mannose, galactose, glucose가 동정되었고, 성숙동안에 pectin질에서는 arabinose, mannose, galactose와 총 비섬유성 중성당의 함량이 감소하였고, 산가용성 hemicellulose에서는 xylose와 mannose가 뚜렸하게 증가하였으나 중성당은 변화없었으며, 알칼리 가용성 hemicellulose에서는 성숙에 따른 변화가 거의 없었다. pectin질의 경우 수용성 pectin, EDTA 용해성 pectin 및 총 pectin은 성숙중에 증가하는 경향이있으나 불용성 pectin은 감소하는 경향이였으며 과숙기에는 불용성 psctin EDTA용해성 pectin 및 총 pectin의 함량은 모두 현저히 감소하였다. 대추의 성숙중 조직에서는 pectin질로 구성된 중충의 붕괴현상이 뚜렸하게 나타났다.발이 절실히 필요한 실정이다. 이러한 배경으로 본 강연에서는 효소적갈변 저해제의 개발과 그들의 식품가공에의 적용 현환 및 화장품, 의약품으로의 응용에 대해 설명하고자 한다.L주에 비해 S주는 수정후 용과가 더 심하다. 9) 화분관의 행동은 수정력과 완전히 일치된다. 즉 L-selfing, $L{\times}L$, S-selfing, $S{\times}S$등의 부적법 수분에서는 화분관은 화주의 미중에서 정지되지만 $L{\times}S$, $S{\times}L$,에서는 수분 약 40-50분 후이면 화분관은 자방까지 도달된다. 10) S주는 웅본으로 오인되어 있지만 인위적법수분을 하면 수정력이나 화분관의 행동은 L주에서와 동일하다. 11) S화분은 완전하지만 L화분은 약 70%가 내용공허한 Adortive pollen 이다. 12) L화분중 나머지 30%도 S화분에 비해서 염색도가 낮은것이 많고 S화분 같이 농염되는 것은 극히 소수이다. 13) 본장물은 분화가 고도로 진행된 전형적인 이형예작물이여 마치 Dimorphism 에서 Dioecious 에로 이행되는 수가 있다는 것을 표시하는 증거가 되는 것 같다. 다소 높은 산소농도 3%~5% 이산화탄소 농도 5~8%에서 저장하는 것이 효과적일 것으로 판단되었다.철쭉군목으로 대표되나 군단이 하의 군목들은 다소 차이를 보이는 것으로 나타났다. 중간상인이론의 수정이 필요하다고 본다.가\ulcorner 본 논문에서는 표면적 형태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$ elements)로 가정한다. 즉, [+wh] 의미의 겹의문사는 동일한 구성요 소를 지닌 병렬적 합성어([

• Proceedings of the Korean Quaternary Association Conference
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• 2004.11a
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• pp.75-76
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• 2004

일산 이산포 BH-4 발굴단면의 퇴적물 시료로부터 화분 포자 및 담수성과 해양성조류화석이 다양하고 풍부하게 산출되었다. 산출된 식물기원 미화석 중, 목본화분 $25{\sim}60%$, 초본화분 $15{\sim}70%$ 그리고 포자 $4{\sim}30%$ 각각 구성 되었다. 또한 담수성 조류(freshwater algae)는 전체 단면구간에서 산출된 반면, 와편모류(dinoflagellate), 아크리타치(acritarch)와 같은 해양성조류(marine algae)는 하부단면구간(절대고도: 3.31-2.74 m)에서만 산출되었다. 산출된 화분 포자 및 통계처리기법(CONISS)을 이용하여 2개의 지역화분대 (local pollen a assemblage zone) 및 4개의 아화분대(local pollen assemblage subzone)가 설정되었다. 제I화분대(시료 $Al-2{\sim}A6-1$, 고도 $2.70{\sim}3.31\;m$)는 다시 제Ia아화분대(시료 $Al-2{\sim}A3-1$, 고도 $2.70{\sim}3.00\;m$)와 제Ib아화분대(시료 $A4-2{\sim}A6-1$, 고도 $3.01{\sim}3.31\;m$)로 세분되었으며; 제II화분대(시료 $A7-2{\sim}A10-1$, 고도 $3.32{\sim}3.72\;m$) 역시 두개의 아화분대인 제IIa(시료 $A7-2{\sim}A8-1$, 고도 $3.32{\sim}3.51\;m$)와 제lIb(시료 $A9-2{\sim}A10-1$, 고도 $3.53{\sim}3.72\;m$)로 이루어졌다. 화분군집조성에 의하면, 미화석들이 퇴적되었던 시기동안에 2개의 산림(forest)형태가 발달했던 나타났다. 제I화분대(고도, $3.31{\sim}2.74\;m$)의 시기동안에는 침엽수와 낙엽활엽수로 이루어진 혼합림으로, 주요 식생은 2엽소나 무(Diploxylon), 가문비((Picea), 낙엽송(Larix)의 침엽수와 참나무(Quercus), 자작나무(Betula), 개암나무(Corylus), 중국굴피 나무속(Pterocarya)의 낙엽활엽수 등이 산악-구릉지에서 주로 서식하였던 것으로 나타났다. 또한 습생나무인 오리나무(Alnus), 버드나무(Salix)등과 해안가 등지에서 잘 서식하는 양치식물과 사초과(Cyperaceae), 명아주과(Chenopodiaceae) 같은 풀들이 하구언(estuary)의 해안가에 주로 서식하였던 것으로 나타났다. 이 시기동안에 해양성조류인 와편모류와 담수성 조류가 동시에 다양하게 산출되는 것은 하구언 퇴적환경이었음을 잘 지시해 준다. 제II화분대(고도, 43.72{\sim}3.36\;m$)가 시작되면서 혼합림은 침엽수림으로 바뀌었으며, 낙엽활엽수들의 서식지가 2엽소나무로 대치되면서 침엽수가 주요 수종을 이루었던 것으로 나타났다. 아마도 이런 산림형태는 자연 기후환경변화 뿐만 아니라 인간간섭에 의해 나타난 결과로 생각된다. 이 시기에 경작식물인 벼과(Gramineae), 옥수수(Zea), 메밀(Fagopyrum)과 논밭의 뚝 등지 에서 서식하는 돼지풀(Ambrosia) 등이 처음으로 산출되는 것으로 보아 인간간섭이 행해졌음을 잘 뒷 받침해준다. 또한 담수성 조류는 변함없이 산출되는 반면, 해양성조류인 와편모류가 산출되지 않는 것으로 보아 해안환경에서 다소 멀 어진 저지대의 습윤한 충적대지환경으로 변하였던 것으로 판단된다.

• The KIPS Transactions:PartD
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• v.10D no.6
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• pp.885-896
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• 2003

The typws of service using location Information are being various and extending their domain as wireless internet tochnology is developing and its application par is widespread, so it is prospected that LBS(Location-Based Services) will be killer application in wireless internet services. This location information is basic and high value-added information, and this information services make prior GIS(Geographic Information System) to be useful to anybody. The acquisition of this location information from moving object is very important part in LBS. Also the interfacing of acquisition of moving object between MODB and telecommunication network is being very important function in LBS. After this, when LBS are familiar to everybody, we can predict that LBS system load is so heavy for the acquisition of so many subscribers and vehicles. That is to say, LBS platform performance is fallen off because of overhead increment of acquiring moving object between MODB and wireless telecommunication network. So, to make stable of LBS platform, in this MODB system, acquisition of moving object location par as reducing the number of acquisition of unneccessary moving object location. We study problems in acquiring a huge number of moving objects location and design some acquisition model using past moving patternof each object to reduce telecommunication overhead. And after implementation these models, we estimate performance of each model.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.119-122
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• 2013

Pollens have been known to possess various biological properties. Therefore, pollens have been extensively used in functional food, folk medicine, and beverage industry to improve human health. This study was conducted to establish the optimized protocol for cytoplasm isolation of bee pollen. Data of biochemical parameters and fatty acid profiles were obtained from pollens of Acorn and Darae(Actinidia arguta). Contents of crude protein and crude fat were 24.1% and 11.8% in Acorn pollen, and those of Darae pollen showed 35.8% and 8.7% in crude protein and crude fat respectively. Also after lyophilizing of Acorn pollen, content of crude protein was increased to 26.5%. Main fatty acids were palmitic acid(C16 : 0), oleic acid(C18 : 1), linoleic acid(C18 : 2) and linolenic acid(C18 : 3) in bee pollen. Linoleic acid(37.3%) was dominant fatty acid in Acorn pollen that is one of essential fatty acids. Linolenic acid(48.3%) was dominant fatty acid in Darae pollen that is a polyunsaturated fatty acid. The proportion of unsaturated fatty acids to total acid content was 73.2% in Acorn and 63.2% in Darae pollen, and especially that of polyunsaturated fatty acids was higher than 55%.

• Korean Journal of Plant Resources
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• v.16 no.1
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• pp.61-73
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• 2003

External and anatomical characters such as stem, leaf, ovary, calyx lobe, ultrastructure of stigma epidermis of leaf blade and midvein, corolla lobe, seed coat and pollen were examined on Pterygocalyx volubilis and Tripterospermum japonicum of Korean Gentianaceae. Fruit characters(capsule and berry), absent or not of corona and pollen characters(reticulate and striate) has been used by identification characters of two taxa. But study results, external characters(root, leaf, calyx lobe, stigma, anther), internal characters(calyx love, ovary) and ultrastructure characters(epidermis o leaf blade and midvein, corolla lobe, seed coat) were useful for species identification. Taxonomic relationships among problematic taxa were discussed.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.35-40
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• 2003

In this study we investigated the effect of gametocides on the number of larger-sized pollen in anther, and also induced callus from the anther culture of Zoysia japonica Steud. Before culturing, we have observed pollens in anther through fluorescence and electron microscopes to know pollen dimorphism. There were two types of pollens observed. One type (30-36 ${\mu}{\textrm}{m}$ in diameter) consisted of vacuolated, larger-sized pollens and the other (15-20 ${\mu}{\textrm}{m}$ in diameter) smaller-sized ones with dense cytoplasm and plenty of amyloplasts. Within few hours, all the smaller-sized pollens were dead, while larger-sized ones were viable for one or two days. To induct larger-sized pollens, various gametocides were leaf-sprayed on three booting stages cultured under 4$0^{\circ}C$ /15$^{\circ}C$ (day/night) before anther culturing. Number of these larger pollens were few (less than 1%) in anther without spraying gametocides. GA$_3$increased the number of larger-sized pollens when applied at mid-booting stage. GA$_3$ with 50 mg/L treatment caused the highest percentage (25.4%) of the larger-sized pollen. Anthers with GA$_3$ treatment were only produced calli on AA medium (modified B$_{5}$+8.0 mg/L 2,4-D ＋0.2 mg/L kinetin), but callus formation was quite low (less than 1%).).