• The Korean Journal of Food And Nutrition
• /
• v.15 no.2
• /
• pp.112-118
• /
• 2002

Sporulation in the fission yeast Schizosaccharomyces pombe has been regarded as an important model of cellular development and differentiation. S. pombe cells proliferate by mitosis and binary fission on growth medium. Deprivation of nutrients especially nitrogen sources, causes the cessation of mitosis and initiates sexual reproduction by matting between two sexually compatible cell types. Meiosis is then followed in a diploid cell in the absence of nitrogen source. DNA fragment complemented with the mutations of sporulation gene was isolated from the S. pombe gene library constructed in the vector, pDB 248' and designated as pDB(spo5)1. We futher analyzed six recombinant plasmids, pDB(spo5)2, pDB(spo5)3, pDB(spo5)4, pDB(spo5)5, pDB (spo5)6, pDB(spo5)7 and found each of these plasmids is able to rescue the spo5-2, spo5-3, spo5-4, spo5-5, spo5-6, spo5-7 mutations, respectively. Mapping of the integrated plasmid into the homologous site of the S. pombe chromosomes demonstrated that pDB(spo5)1, and pDB(spu5)Rl contained the spo5 gene. Transcripts of spo5 gene were analyzed by Northern hybridization. Two transcripts of 3.2 kb and 2.5kb were detected with 5kb Hind Ⅲ fragment containing a part of the spo5 gene as a probe. The small mRNA(2.5kb) appeared only when a wild-type strain was cultured in the absence of nitrogen source in which condition the large mRNA (3.2kb) was produced constitutively. Appearance of a 2.5kb spo5-mRNA depends upon the function of the meil, mei2 and mei3 genes.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.23 no.4
• /
• pp.247-254
• /
• 2003

A study was carried to compare two varieties of tall fescue(Festuca arundinacea Schr.): Fawn (endophyte infection) and Roa(endophyte-free). The study examined plant height, botanical composition, and dry matter yield of mixed pasture. The cattle grazed on a mixed pasture of tall fescue species and orchardgrass(Dactylis glomerata L.), perennial ryegrass(Lolium perenne L.) and white clover(Trifolium repens L.) during March 22, 1997, to September 21, 1997. The number of grazing animals was 4.3/ paddock(50m x50m) and was adjusted according to the condition of pasture. Dry matter yields determined on ungrazed pasture(7-time harvests) were 1,690 $\pm$407kg and 1,128 $\pm$ 238kg/ha on pastures consisting of Fawn and Roa, respectively. This difference was significant(P<0.01). There were 17.71 $\pm$ 1.27cm and 12.83$\pm$0.90cm in average plant lengths of Fawn and Roa(P<0.01), respectively The botanical composition of tall fescue and orchardgrass in the mixture treatment included in the Fawn variety were shown in 34.2 and 3.8％, comparing Roa with 17.1 and 9.6％, on the 21th of September, 1997, respectively. Tall fescue Roa represented a higher botanical composition than orchardgrass(a main species on Cheju). Crude protein content of Fawn was slightly less than that of Roa. From these results, we may conclude that Roa variety is nutritionally better, but is less adaptible to high temperature climate than Fawn. However, Roa variety is still better than orchardgrass in high temperature conditions.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.14 no.4
• /
• pp.307-315
• /
• 1994

The object of this experiment was to suggest the suitahle N-P-K fertilization level for orchardgrass(0G)-red clover(RC) mixtures under the intensive system of short-term pasture utilization. The fields trials were conducted over 3-year period(1991-1993) to evaluate dry matter yield. botanical composition, chemical composition and CP, DDM yield on the N-P-K levels (0-0-0. 50-100-100. 100-150-150, 150-2OO-200. 200-250-250 and 300-350- 350kg/ha). With increasing level of N-P-K, the DM yield of OG in the OG-RC mixture were significantly increased(P< 0.05), however, that of RC was remarkably decrcawd(P

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.5 no.3
• /
• pp.191-195
• /
• 1985

This field experiment was undertaken to assess the effects of three levels of inclination ($10^{\circ},\;20^{\circ},\;and\;30^{\circ}$) and four rates of $N-P_2O_2-K_2O$ (O-O-O, 14-10-20, 28-25-25, and 42-40-40kg/10a) on establishment, forage yield and quality and botanical composition of new established mixed grass-clover sward. This first part is concerned with the forage yield, yield component and botanical composition. The results obtained after a two-year experiment are summarized as follows: 1. Dry matter yields of mixed grass-clover sward were greatly decreased by increasing the inclination grade of hilly pasture, whereas it of weeds was not significantly influenced. Comparing with weeds, the establishment of introduced pastures was relatively low with increasing the grade of inclination. 2. By increasing the NPK rate, the yields of grasses and mixed forages and the rate of introduced pastures were greatly increased at all grades of inclination, whereas the change in the weed yield was not significant. 3. There were the differenced in the forage yield and yield component, and efficiency of fertilizers by the grade of inclination, which indicated the necessity of different management on the selection of suitable grass species, seeding rate, amount of fertilizer application, and the methods of grassland establishment, management and utilization.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.31 no.1
• /
• pp.33-38
• /
• 2011

This experiment was conducted to compare the dry matter yield and forage quality according to the seed mixture of annual legumes and Italian ryegrass (Lolium multiflorum, IRG) on paddy field at the experimental field of Jeollanam-Do Agricultural Research and Extension Services from 2007 to 2008. The five treatments used in this experiment were IRG single (cv. Kowinearly), IRG+Chinese milk vetch mixture, IRG + Crimson clover (cv. Linkarus) mixture, IRG + Austrian winter pea (Pisum sativum subsp. arvense cv. forage Pea) mixture and IRG + Hairy vetch (Vicia villosa. cv. Oregon Common) mixture. And the mixing ratio of Italian ryegrass + annual legumes were 70 : 30 as based mono seed rate. The dry matter (DM) percentage at harvest was 23.6~26.8%. The DM percentage among IRG single and mixture treatments were similar. The yields of fresh and CP were high in IRG + Forage pea mixtures as a 40,100 kg and 625 kg per ha, respectively (p<0.05). The yields of dry matter were high in IRG + Forage pea and IRG + hairy vetch pea mixtures as a 9,470 kg and 9,500 kg per ha, respectively (p<0.05). But the forage quality did not show difference between Italian ryegrass mono-culture and annual legumes mixture. The Av. $P_2O_5$ in IRG+Forage pea mixture was 78 mg/kg and concentration of K were 0.52~0.88. In conclusion, the mixture of IRG and Forage pea, dry matter yield, protein yield increased, and was effective in enhancing the stability of cultivation.

• Korean Journal of Soil Science and Fertilizer
• /
• v.14 no.1
• /
• pp.31-36
• /
• 1981

This pot experiment was conducted to find out the optimal fertilization ratio of the anions (N : S : P) in various soil conditions. Soil samples were collected from uncultivated mountains and hills where development for grasslands is under consideration. The optimum ratios were computed by the $Hom{\grave{e}}s$ systematic variation technique. The results were summarized as follows : 1. The optimum fertilization ratios of the anions (N : S : P) for the high yields of grass - clover mixed sward at various areas were obtained (Table 5). 2. The grass yields in mixed sward were increased by the increase of specific soil characteristics, CEC, OM, T-N, avail-$P_2O_5$. However. it was not recognizable by the contents of the cations, the base saturation, and the pH. 3. The relatively high yields of clover were positively related to the Ca and Mg contents in soils, and it was a factor of the increased total yields of mixed sward. 4. The relatively high optimum fertilization ratios of S in volcanic ash soil in the Cheju district and P in sediment lithosol in Daegu should be taken into account in determing the fertilization rate for high yield of clover in mixed sward.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.23 no.2
• /
• pp.81-90
• /
• 2003

This pot experiment was conducted to find out the effects of boron application(B$_{0}$; 0.0, B$_1$; 0.2, $B_2$;2.0, $B_3$;6.0, $B_3$;6.0, $B_4$;15.0me B/pot) on the forage performance in the pure and mixed swards of orchardgrass and white clover. This 1st part was concerned with the changes in the growth, flowers and flower buds, and roots/nodules of forages. The results obtained are summarized as follows: 1. At the $B_3$ and $B_4$ treatments, the B toxicity was more seriously in white clover than in orchardgrass in the first half of cutting orders, and reduced in the latter half. In white clover, it was more worsened in a mixture than in a pure culture. It was somewhat reduced at the best growth stage of each forage. 2. In orchardgrass, the B toxicity($B_3$,$ B_4$) showed the chlorosis on leaf tips, shallow leaf, little tillers, and weak stems. Whereas it showed the chlorosis/necrosis on old leaf edge, little and weak stolons in white clover. 3. Comparing with the B deficiency($B_{0}$ , $B_1$) and toxicity($B_3$, $B_4$), the optimum B application($B_2$) influenced markedly good growth of shoot, root, nodule, and flower (flower number, blooming period, early full flower) in white clover. 4. Comparing with orchardgrass, white clover was greatly influenced by the boron application. However, this responses of white clover to boron were reduced in a grass-clover mixture with additional fertilization. It was recognized that the good forage performance in a grass-clover mixture could be regulated by the adequate applications of boron and additional fertilizers.s.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.5 no.1
• /
• pp.53-61
• /
• 1985

Effects of nitrogen incorporated with 15N-nitrogen, which was applied to grown alone and in mixture with grass and legumes of forage crops, on the yield of dry matter, total nitrogen content, percentage of nitrogen derived from fertilizer (P.N.D.F.), A-value were studied, and the results obtained are as follows: 1. With nitrogen for the yield of dry matter was highly effective only to orchardgrass and were also effect to the early stages of alfalfa and birdsfoot trefoil in case of a mixed sowing. 2. Alone and mixed sowings showed the yield of dry matter in decreasing order of alfalfa<red clover>orchardgrass>birdsfoot trefoil, and orchardgrass + alfalfa>orchardgrass + red clover>orchardgrass + birdsfoot trefoil, respectively. 3. In case of grown alone, orchardgrass gave higher percentage of nitrogen derived from fertilizer (P.N.D.F.) than legume, whereas in case of grown in mixture it was in reverse order throughout the plots. It appears likely that orchardgrass was rather supplied soil nitrogen by legume then by nitrogen application. And P.N.D.F. was getting smaller in the latter stage of all forage crops in case of grown alone. In case of grown in mixture, however, and inconsistent P.N.D.F. was obtained from orchardgrass. 4. The A-value in case of grown alone was decreased in order of alfalfa>red clover>orchardgrass>birdsfoot trefoil. In contrast, however, the A-value in case of grown in mixture wes decreased in order of orchardgasss+alfalfa (3:7)>orchardgrass+red clover (3:7)>orchardgrass+red clover (3:7)>orchardgrass+alfalfa(5:5)>orchardgrass+birdsfoot trefoil (5:5)>orchardgrass+red clover (5:5)>orchardgrass+birdsfoot trefoil (3:7).

• Journal of Animal Environmental Science
• /
• v.6 no.1
• /
• pp.53-58
• /
• 2000

A manure management plan is important for all daily operations. This study was conducted to investigate the effect of application level of liquid cattle manure on the soil properties and changes of BOD and COD content in run-off water at the mixtures swards of National Livestock Research Institute RDA Suweon in 1995. The experiment was arranged in a randomized block design with five treatments consisting of no fertilizer chemical fertilizer application of 40, 60 and 80MT/ha as liquid cattle manure. The quantity of run-off for a no fertilizer was the highest of 1,469.4mm but that for a application level of liquid cattle manure of 60MT/ha was the lowest of 1,278.1mm. The change of BOD in run-off for a no fertilizer was the lowest of $19.84m{\ell}/{\;}{\ell}$, but that for a application level of liquid cattle manure of 80MT/ha was the highest of $36.22m{\ell}/{\;}{\ell}$. Change of COD in run-off for a no fertilizer was the lowest of $21.28m{\ell}/{\;}{\ell}$ but that for a application level of liquid cattle manure of 80MT/ha was the highest of $37.51m{\ell}/{\;}{\ell}$. Available phosphorus and total-N content of soil chemical properties was higher at liquid cattle manure than chemical fertilizer.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.43 no.2
• /
• pp.88-94
• /
• 2023

This study was conducted to analyze and compare the dry matter yield of Italian ryegrass (IRG) cultivated under monoculture and mixed culture system to recommend suitable varieties that can be cultivated. Italian ryegrass cultivars, Green Fram (GF, extremely early-maturing), Kowinearly (KE, early-maturing), Kowinmaster (KM, mild-maturing), and Hwasan 104 (H104, late-maturing), were used for mono or mixed cultivation. The average monthly temperature in Cheonan over the past 30 years tended to be similar, but that in November and March are judged to be abnormal weather. The dry matter yield of GF+H104 was significantly higher during harvest than that of GF (p<0.05). The dry matter yields of KE and KE+KM were significantly higher during harvest than the output standards of KE and KM. There was no significant difference between the dry matter yield of H104 and KM (p>0.05), but KM had the highest yield of 16,763.1 kg/ha. Analysis showed that the highest dry matter yield during IRG harvest was obtained under monoculture and KE+KM mixed culture. Because the occurrence frequency of abnormal weather such as drought during spring is increasing recently, it is judged that IRG cultivation using early and middle growth is necessary to prepare for abnormal weather.