• Title/Summary/Keyword: 형광변수

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Laser Induced Microjet Drug Delivery System: Drug Permeation Depending on Laser Wavelength and Pulse Duration (레이저 유도 마이크로젯을 활용한 약물 전달 방식: 레이저 파장 및 펄스길이에 따른 약물 침투 분석)

  • Jang, Hun jae;Ham, Hwi chan;Yoh, Jai ick
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.41 no.7
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    • pp.463-468
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    • 2017
  • For transdermal drug delivery, needless injection system is composed of laser and microjet injector. Main mechanism of microjet injector is the laser-induced bubble. Nd:YAG and Er:YAG laser are used as a power source. Laser parameters such as pulse duration and wavelength are considered, which are core parameters to control the bubble motion. The Nd:YAG laser, pulse duration is short than bubble life time making cavitation like bubble while in Er:YAG laser, long pulse duration and high absorption in water drive bubble as a boiling bubble. Detailed motion of bubble and microjet is captured by the high speed camera. So it is observed that microjet characteristics are determined by the bubble behavior. The performance of drug delivery system is evaluated by fluorescent staining of guinea pig skin.

Design studies for mandrel type fiber-hydrophones with FEM (FEM을 이용한 맨드랠(Mandrel) 형광- 음향 수중 청음기의 설계에 관한 연구)

  • Im, Jong-In;Roh, Yong-Rae
    • The Journal of the Acoustical Society of Korea
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    • v.16 no.3
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    • pp.73-80
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    • 1997
  • This paper describes structural optimization of optical fiber-wound mandrel hydrophones with Finite Element Method (FEM). The hydrophone is supposed to have operation frequency range of up to 10 kHz and show omni-directional sensitivity pattern at 5 kHz. Studied parameters are mandrel geometry, molding thickness, and material properties of constitutional parts of the hydrophone. Theoretical calculation result shows that pressure sensitivity of the hydrophone increased as either mandrel length or molding thickness gets larger. Also higher pressure sensitivity requires a mandrel or molding material with relatively low Youngs modulus or Poissons ratio. Hydrophone bandwidth increases either as the mandrel length becomes shorter or as the mandrel becomes harder. The omni-directional characteristic is improved as the mandrel length becomes shorter, at 5 kHz. With the above results, we determine the structure of an optical fiber-wound mandrel hydrophone which has the pressure sensitivity of $30 {\times} 10_{-7}$ Rad./Pa, operation frequency range of up to 10 kHz, and shows omni-directional sensitivity pattern at 5 kHz.

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Chemical Washing of PAH-Contaminated Soil with Cyclodextrins as a Main Surfactant: A Labscale Study (사이클로덱스트린을 이용한 PAH오염토양의 화학적 세정)

  • Sung Hyun Kwon;Daechul Cho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.3 no.4
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    • pp.295-302
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    • 2002
  • PAHs (polycyclic aromatic hydrocarbons) deposited in soil are one of serious problems against sustainable land use. In this paper, chemical soil flushing in a packed sandy soil matrix using a natural surfactant, $\beta$-cyclodextrin (CD) was studied via a fluorescence spectroscopy and a dye labelling. The contaminants are lipophilic ring compounds- phenanthrene and naphthalene. Sand type and flushing intensity (rate and concentration) are chosen as important investigation variables. The removal efficiencies were proportional to flow rate, concentration, temperature of the flushing solution and voidity of the sand column. Initial sorption of the surfactant onto the soil matrix was found to be a key step while flow shear was more crucial in the latter steps. The residual portion of the surfactant, which was most likely to be due to the initial sorption, would not be so influential on this type of soil washing for long times. These results will be useful in future for pilot scale in situ washing and for establishing better soil washing strategy.

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Fundamentals and Applications of Multi-functional NSOM Technology to Characterization of Nano Structured Materials (다기능 NSOM (mf-NSOM) 을 이용한 나노 구조 재료 분석에 관한 원리와 응용)

  • Lee Woo-Jin;Pyun Su-Il;Smyrl W. H.
    • Journal of the Korean Electrochemical Society
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    • v.7 no.2
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    • pp.108-123
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    • 2004
  • Imaging of surfaces and structures by near-field scanning optical microscopy (NSOM) has matured and is routinely used for studies ranging from biology to materials science. Of interest in this review paper is a versatility of modified or multi-functional NSOM (mf-NSOM) to enable high resolution imaging in several modes: (1) Concurrent fluorescence and Topographical Imaging (gases) (2) Microspectroscopy (gases) (3) Concurrent Scanning Electrochemical and Topographical Imaging (SECM) (liquids) (4) Concurrent Photoelectrochemical and Topographical Imaging (PEM) (liquids) The present study will summarize some of the recent advances in mf-NSOM work confirmed and supported by the results from several other imaging techniques of optical, fluorescence, electron and electrochemical microscopy. The studies are directed at providing local information on pitting precursor sites and vulnerable areas on metal and semiconductor surfaces, and at reactive sites on heterogeneous, catalytic substrates, especially on Al 2024 alloy and polycrystalline Ti. In addition, we will introduce some results related to the laser-induced nanometal (Ag) synthesis using mf-NSOM.

Fabrication of $CaSO_4:Eu$ TLD and Its Physical Characteristics ($CaSO_4:Eu$ TLD의 제작과 물리적 특성)

  • Kim, Do-Sung;Park, Myeong-Hwan
    • Journal of Sensor Science and Technology
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    • v.8 no.5
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    • pp.388-393
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    • 1999
  • In this study, the $CaSO_4:Eu$ TLDs are fabricated and their trap parameters are determined. The optimum concentration of Eu for fabrication of the $CaSO_4:Eu$ TLD is 0.5 mol% and optimum temperature is $600^{\circ}C$ for 2 hours sintering in air. The glow curve of $CaSO_4:Eu$ consists of two glow peaks and these peaks are isolated by thermal bleaching method. Trap parameters of two glow peaks are measured using the initial rise, the peak shape, the heating rate and the least square curve fitting methods. The activation energies of the glow peak I and II are 1.00 eV and 1.09 eV, and the frequency factors are $7.04{\times}10^{11}\;s^{-1}$ and $5.12{\times}10^{11}\;s^{-1}$ and the kinetic orders are 1.11 and 1.33, respectively.

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Effect of Irrigation Starting Point of Soil on Chlorophyll Fluorescence, Stem Sap Flux Relative Rate and Leaf Temperature of Cucumber in Greenhouse (시설 토양 오이재배에서 관수개시점 처리가 광합성 형광반응, 줄기수액흐름 및 엽온에 미치는 영향)

  • An, Jin Hee;Jeon, Sang Ho;Choi, Eun Yong;Kang, Ho Min;Na, Jong Kuk;Choi, Ki Young
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.46-55
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    • 2021
  • This experiment was conducted to investigate the effect on chlorophyll fluorescence, stem sap flux relative rate (SFRR) and leaf temperature of cucumber when irrigation is controlled using a soil moisture tensiometer. Cucumber (Cucumis sativus L.) 'Chungchun' was irrigated of 10-10-20 kPa and 20-10-10 kPa by soil starting point of irrigation at each growth stage. At the 66 days after treatment (DAT) of 736 to 854 W·m-2 and above 32℃, chlorophyll fluorescence variables (Fo, Fm, Fv/Fm) values showed significantly different between treatments. The Fo and Fv/Fm value in the daytime (10:30 am to 6:00 pm) at 66 DAT was higher in 20-10-10 kPa treatment than in 10-10-20 kPa treatment. The Fv/Fm value decreased when the leaf temperature was increased. There was no difference in leaf growth (length, width and area) at 28 and 66 DAT, but the chlorophyll content (SPAD value) was significantly higher in 20-10-10kPa treatment. SFRR and leaf temperature increased with light intensity and temperature increased. In both treatments, the SFRR started to increase sharply between 8 am and 9 am when the solar radiation is 170 W·m-2 or higher. The soil temperature of the treatments decreased after irrigation, that showed 31.0℃ at 10-10-20kPa and 28.5℃ at 20-10-10kPa on July 5 (820W·m-2 at 1 pm). However, there was no difference in SFRR, leaf temperature, temperature difference (leaf temperature - air temperature) and VPD between treatments. SFRR was significantly positive correlate with the leaf temperature (p < 0.01, r = 0.770). The SFRR and leaf temperature showed positive significant correlation with solar radiation, temperature, soil temperature, soil moisture content and VPD. There was a negative significant correlation with relative humidity and temperature difference.

Sensibility Evaluation on the Correlated Color Temperature in White LED Lighting (백색 LED 조명의 색온도에 관한 감성평가)

  • Jee, Soon-Duk;Lee, Sang-Hyuk;Choi, Kyoung-Jae;Park, Joung-Kyu;Kim, Chang-Hae
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.22 no.4
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    • pp.1-12
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    • 2008
  • The aim of this study is to investigate the sensitivity evaluation of human beings in reacting to the correlated color temperature of the optical properties of white LED lighting. For the sake of this study, white light-emitting diode modules have been fabricated their correlated color temperature have been measured, test cabinets for the sensitivity evaluation have been constructed with the white LED modules, and their sensitivity reactions on the test cabinets have been evaluated and analyzed. The sensitivity reaction has been evaluated by the semantic differential method with 15 selected questions, and the reliability and the content validity of their lighting have been analyzed to 3 factors which foe the activity as the first factor, the stability as the second one, the potency as the third one, respectively. For the data analysis on the sensitivity reaction, the dependent variable is the score of the sensitivity evaluation and the independent one is the correlated color temperature of the test module. The results of this study is as follows: In the case of the sensitive evaluation on the activity and the potency in the white LED lighting compared with the fluorescent lamp, the subjects have made higher mark on $MA_3$ with 8,300[K], and in the factor of the stability, they have made higher mark on $MA_1$ with 3,800[K].

Mouse Embryonic Stem Cell Uptakes of Buforin 2 and pEP-1 Conjugated with EGFP (생쥐 배아 줄기세포의 Buforin 2 및 pEP-1에 결합된 EGFP의 세포 내 수송)

  • Jung, Su-Hyun;Park, Seong-Soon;Lim, Hyun-Jung;Cheon, Yong-Pil
    • Development and Reproduction
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    • v.11 no.2
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    • pp.111-119
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    • 2007
  • Differentiation of cells can be induced through modulation of endogenous regulators using exogenous factors. Useful transfection systems to transport a specific exogenous regulator into cell have been tried but still there are many obstacles to overcome. In this study, we examined the transfection efficiency of cell permeable peptides (CPPs) in mouse embryonic stem cell under the various conditions. To identify the CPP-mediated translocation of a protein, we employed recombinant CPP-enhanced green fluorescent protein (EGFP). Viability of R1 cells was different between experimental groups depending on the kind of CPP and the concentration of CPP-EGFP. Translocation of CPP-EGFPs into the R1 cells was not detected until 30 min after CPP-EGFPs treatment in all groups. After 1 hr, translocation of pEP-1-EGFP-N was detected, but it could not be detected in the other group. Transfection of pEP-1EGFP-N was independent on its concentration. The time course did not show saturation even after 24 hr in pEP-1-EGFP-N. These results showed that the permeability depended on the kind of CPP and the location of His-tag in the case of examined CPPs, and did not need biological energy. On summary, the efficiency of transfection of CPP-EGFP depends on the CPP sequences but the culture time is not a key factor in transfection for the mouse embryonic stem cell. For the future studies to improve the efficiency of translocation of protein into embryonic stem cells, it is needed to develop modified CPP or mediator. The studies would be very useful to induce the differentiation of embryonic stem cells.

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Responses of Photosynthetic Characters to Waterlogging in Soybean [Glycine max (L.) Merrill] (과습에 따른 콩 광합성 관련 형질 반응)

  • Lee, Jae-Eun;Kim, Hong-Sig;Kwon, Young-Up;Jung, Gun-Ho;Lee, Chun-Ki;Yun, Hong-Tai;Kim, Chung-Kon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.2
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    • pp.111-118
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    • 2010
  • Stress due to excess water is one of the most limiting factor for soybeans to high yield under wet climates. This study aimed to identify the photosynthetic responses of soybeans to waterlogged growing condition with 5 soybean varieties by waterlogging for 10 days at V5 and R2 stage, respectively. Chlorophyll fluorescence decreased more rapidly at R2 stage waterlogging than at V5 stage waterlogging in all soybean tested varieties. The degree of recovery was much more in Pungsannamulkong and Muhankong( 95~97% of control) than in Jangyeobkong and Myungjunamulkong at 5 days after waterlogging. Photosynthetic rate, transpiration and stomatal conductance were also increased more rapidly in Pungsannamulkong and Muhankong than in Jangyeobkong and Myungjunamulkong after waterlogging irrespective of waterlogging stages. As the result of multiple regression analysis in order to identify the effects of stomatal conductance and transpiration to the photosynthetic rate, the R2 value of stomatal conductance in control and waterlogging treatment was 0.7293 and 0.7582, respectively. If the transpiration, another dependent variable, was added to the regression formula, there was not so big difference in the variation of photosynthetic rate. This result means that if just one factor of them(the stomatal conductance and transpiration) be measured in the case of waterlogged condition, the changes of photosynthetic rate can be estimated.

Photosynthesis, Growth and Yield Characteristics of Peucedanum japonicum T. Grown under Aquaponics in a Plant Factory (식물공장형 아쿠아포닉스에서 산채 갯기름의 광합성, 생육 및 수량 특성)

  • Lee, Hyoun-Jin;Choi, Ki-Young;Chiang, Mae-Hee;Choi, Eun-Young
    • Journal of Bio-Environment Control
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    • v.31 no.1
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    • pp.67-76
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    • 2022
  • This study aimed to determine the photosynthesis and growth characteristics of Peucedanum japonicum T. grown under aquaponics in a plant factory (AP) by comparing those grown under hydroponic cultivation system (HP). The AP system raised 30 fishes at a density of 10.6 kg·m-3 in a 367.5 L tank, and at HP, nutrient solution was controlled with EC 1.3 dS·m-1 and pH 6.5. The pH level ranged from 4.0 to 7.1 for the AP system and 4.0 to 7.4 for the HP system. The pH level in the AP began to decrease with an increase in nitrate nitrogen (NO3-N) and lasted bellower than pH 5.5 for 15-67 DAT. It was found that ammonium nitrogen (NH4-N) continued to increase even under low pH conditions. EC was maintained at 1.3 to 1.5 dS·m-1 in both systems. The concentration of major mineral elements in the fish tank was higher than that of the hydroponics, except for K and Mg. There was no significant difference in the photosynthesis characteristics, but the PIABS parameters were 30.4% lower in the AP compared to the HP at the 34DAT and 12.0% lower at the 74DAT. There was no significant difference in the growth characteristics, but the petiole length was 56% longer in the leaf grown under the AP system. While there was no significant difference in the fresh and dry weights of leaf and root, the leaf area ratio was 36.43% higher in the AP system. All the integrated results suggest that aquaponics is a highly-sustainable farming to safely produce food by recycling agricultural by-products, and to produce Peucedanum japonicum as much as hydroponics under a proper fish density and pH level.