• Korean Journal of Veterinary Service
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• v.13 no.1
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• pp.69-74
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• 1990

The present study was conducted to investigate the incidence of pasteurella multocida infection in kyungbuk swine herds during the period from July 1989 to November 1989 and some properties of the isolated organisms. P. multocida was isolated from lungs of 155 slaughtered pigs, 43(27.7%) pigs were culture positive. The majority of biochemical and cultural properties of the P. multocida isolates were identical to those of the standard strains. The capsular serogroups and drug susceptibility of 43 isolates of P. multocida from pigs with pneumonic lesions were investigated. P. multocida isolates were typed for capsular serogroupes A by hyaluronidase inhibition of capsule and D by acriflavin auto agglutination. Most isolates(60.4%) were type A, 18.6% were type D, and the remaining 21.0% were untypable. In antimicrobial susceptibility test these isolates of P. multocida were susceptible in order of ampicillin (86.0%), trimethoprim sulfamethoxazole(83.7%), colistin(81.4%), chloramphenicol(79.1%), but the majority of them were resistant in order of streptomycin(30.2%), triple sulfa (4.6%).

• Korean Journal of Microbiology
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• v.27 no.3
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• pp.231-236
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• 1989

The larger segment of double stranded RNA genome from a new serotype of Infectious Pancreatic Necrosis Virus (IPNV), DRT, has been partially cloned at Sma I site in pUC19 and compared with the restriction maps of VR-299 and Sp. Restrction sites found in DRT was distinct and hence a new serotype. The cDNA clones of DRT were about 800, 850, and 1, 400 bp long each and do not share any common restiction site. It is not clear yet if there exist any overlapping sequences among them. This partial cloning, however, was sufficient for the comparison of restriction maps with the other serotypes.

• Journal of Periodontal and Implant Science
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• v.26 no.2
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• pp.365-375
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• 1996

5 serotypes(a, b, c, d, e) of Actinobacillus actinomycetemcomitans showed distinct hybridization patterns(DNA fingerprinting patterns) when the bacterial DNA were hybridized with randomly cloned 4.7-Kb sized DNA probe. The sizes of hybridized bands in each serotypes were different among serotypes and represented unique patterns of hybridization with the probe used. The serotype a showed two bands of fingerprinting patterns: 23.1 kb and 2.5 kb respectively. Serotype b and c showed single band: 6.6 kb and 9.5 kb, respectively. Serotype d and e showed two bands of hybridization: 23.1 kb and 2.8 kb, and 23.7 kb and 2.1 kb, respectively. The results indicate that this standard fingerpriting patterns of DNA hybridization with 4.7 kb probe can be further used for genotyping clinical isolates of Actinobacillus 8ctinomycetemcomitansand its relevance with periodontal disease activity.

• Animal Systematics, Evolution and Diversity
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• v.6 no.2
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• pp.165-172
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• 1990

The patterns of serum proteins in seven species of Korean rodents were analyzed by immunoprecipitin, immunodiffusion, and immunoelectrophoresis. It is found that the serum proteins of each species were different with one another and thatntigenic divergence among the seven species seems to be enormous , ie., serological correspondence ranged from 99.6 in Rattus norvegicus caraco(suborder Myomorpha) to 2.7 in Tamias sibircus asiaticus (suborder sciuromorpha). In the six species of the family Muridae (Suborder Myomorpha), the lowest value of 8.2 was shown in Clethrionmys rufocanus regulus (Subfamily Microtinae).

• Korean Journal of Veterinary Service
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• v.25 no.3
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• pp.259-273
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• 2002

The present study was conducted to investigate the prevalence, biochemical properties and serotypes of Salmonella organisms in the domestic animals in Western Gyeongbuk province during the period from January to December 2000. Salmonella spp were isolated from 51(1.62%) of 3,141 cases of domestic animals. Serotypes of isolates were S enteritidis 17(29.6% of isolates), S agona 11(20.3%), S rissen 8(14.8%), S gallinarum 4(7.4%), S derby 3(5.5%), S typhimurium 2(3.7%), S travis 1(1.8%), S montevideo 1(1.8%) and untypable 4(12.9%), in order. The majority of isolates were highly susceptible to amikacin, ampicillin, ciprofloxacin, cephalothin, chloramphenicol, trimethoprim-sulfamethoxazole, norfloxacin and gentamicin, whereas all isolates were resistant to bacitracin, erythromycin, tiamulin and tylosin, and the majority of them were highly resistant to penicillin, streptomycin and tetracycline.

• Korean Journal of Microbiology
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• v.23 no.4
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• pp.291-296
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• 1985

Leptospira isolated from patients and natural paddy water were further studied to confirm their serologic specificity with the bacteria in infected animal tissue and autopsied tissue of patients died with leptospirosis. And pathologic patterns of the inoculated antimal and the virulence of the bacteria in the animals were also studied. The findings are summarized as follows; $LD_{50}$ dose of the bacteria in guinea-pig were $1-2{\times}10^9$ cells and mice were found to be susceptible to them even though the degree of susceptibilities were much inferior to guinea pig. The bacteria were recovered from various organs; demonstrated massive hemorrages due to diapedesis and monocyte infiltration were observed, in some cases, intramedullary hemorrages of the infected kidnies and hematuria were recognized. All of the hyperimmune sera were strongly reacted with the tissues from autopsied human case who died of EPHF(Epidemic Pulmonary Hemorrhagic Fever) in immunofluorescent antibody tests.

• Neonatal Medicine
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• v.15 no.1
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• pp.44-53
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• 2008

Purpose : Epidemic keratoconjunctivitis (EKC) caused by adenovirus is a highly contagious disease, which has been reported as outbreaks involving adults in the community. However, there has been no report on EKC outbreak by adenovirus in a neonatal intensive care unit (NICU) in Korea. Aims of this study were to investigate the EKC outbreak by adenovirus type 8 in NICU and to confirm an effectiveness of polymerase chain reaction (PCR) for diagnosis. Methods : Conjunctival swab or nasopharyngeal aspirate specimens were taken from all patients and tested by viral culture and PCR. Adenovirus serotype was determined by sequencing of PCR product of selected region of hexon gene using the virus isolates or specimens. Results : An outbreak of EKC occurred which was involving 12 preterm infants in the NICU of the Seoul National University Children's Hospital between July 12th and August 1st, 2005. Three hospital staffs and one family member of the neonate were also affected. Adenovirus was detected in 12/12 (100%), 6/11 (54.5%) by PCR and virus culture, respectively. Eleven PCR-positive neonates were identified as serotype 8 by sequencing. The first affected 4 babies have had routine ROP (retinopathy of prematurity) examinations one week ago. While previous outbreaks were sustained for a few months, the event in our unit was controlled without complications in 3 weeks. Conclusion : We analyzed the EKC outbreak by adenovirus type 8 in NICU. Adenovirus serotype was identified by PCR and sequencing with high sensitivity for the first time in Korea, so we suggest this method can be very useful for rapid diagnosis and infection control.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1210-1214
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• 2001

To determine the effects of dietary restriction on obese type 2 diabetes we measured body weight, blood glucose and serum lipid level in dietary restricted Otsuka Long Evans Tokushima Fatty (OLETF) rats. OLETF rats (obese diabetic rats) and LETO rats (control rats) were grouped into 3 groups; control (free feed) group, 20% dietary restricted (20% DR) group and 40% dietary restricted (40% DR) group. Body weight of rats was measured every weeks and the level of glucose, triglyceride (TG), total cholesterol (TC) and HDL-cholesterol in blood of rats were also determined at 12 weeks after dietary restriction. Body weight of control, 20% DR and 40% DR groups were increased by 41%, 20% and 10%, respectively in LETO rats and by 24%, 10% and -2%, respectively in OLETF rats. Blood glucose level of LETO rats were decreased by 12% on 40% DR compared to control group but the differences between control group and 20% DR group was not observed. The blood glucose level of OLETF rats were decreased by 20% in 40% DR group and by 15% in 20% DR group. The levels of blood triglyceride in 20% DR and 40% DR group were decreased by 20%, 15% in LETO rats and by 37%, 32% in OLETF rats, respectively Total cholesterol revel was not changed by dietary restriction in LETO rats, but significant changes were observed in OLETF rats by both 20% and 40% dietary restriction. HDL-cholesterol levels were also increased by dietary restriction in both LETO and OLETF rats. These results suggested that body weight and blood glucose, serum triglyceride and total cholesterol levels were decreased by dietary restriction and these changes are more susceptive in diabetic rats than non-diabetic animals.

• Journal of Veterinary Clinics
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• v.27 no.3
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• pp.246-251
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• 2010

Mastitis is an inflammation of the mammary gland that develops as a response to pathogenic microorganisms. Somatic cell count (SCC) and bacteriological analysis are the most accepted tools to determine udder health. This study evaluated the effects of intra-mammary gland infusion of silver ion against clinical mastitis in Holstein cows. Silver ion (20 ${\mu}g$/ml) was infused through the intra-mammary gland in quarters having clinical mastitis and milk was collected to determine SCC, and levels of lactoferrin and bovine serum albumin (BSA). Silver ion infusion decreased udder inflammation, firmness and swelling, and reduced clots, BSA, lactoferrin and SCC in milk. However, milk yield and circulating blood cells remained unchanged. The silver ion-mediated reductions of BSA and SCC indicate reduced inflammation and bacterial activity in silver ion-treated mammary glands in Holsteins with mastitis, which may be exploited in a curative strategy.

• Parasites, Hosts and Diseases
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• v.31 no.2
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• pp.157-164
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• 1993

Acanthamoebn culbertsoni is a pathogenic free-living amoeba causing primary amoebic meningoencephalltls (PAME) in human and mouse. Several reports on the immune responses in mice with this amoebic infection have been published, but the effects of transferred passive Immunity on the active immunization In offspring mice have not been demonstrated. This experiment was done to observe the effect of active Acanthamoebn culbertsoni was cultured in the CGV medium axenlcally. Female BALB/c mice weighing about 20g were immunized through the intraperitoneal injection of Acanthamoeba cuLbensoni trophozoites 1 × 106 each three times at the interval of one week. Offspring mice were immunized two times. The mice were inoculated Intranasally with 1 × 104 trophozoites under secobarbital anesthesia. There was a statistical difference in mortality between the transferred immunity group and the active immunization group. Statistical differences were not demonstrated in antibody titer between both groups. But L3T4+ T ce11/Ly2+T cell ratio was increased in the transferred Immunity group more than active immunization group of the offspring mice at the age of 5 weeks. There was no differences statistically in mortality between both groups. It was recognized that active immunization in offspring mice born to immune mother could modulate the immune status according to the time of Immunization.