• Journal of Nutrition and Health
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• v.48 no.2
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• pp.140-148
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• 2015

Purpose: The purpose of this study was to evaluate the role of coffee in diabetic rats in order to prevent hyperglycemia and hyperlipidemia, and to improve antioxidant enzyme activity in streptozotocin induced diabetic rats. Methods: Thirty two male Sprague-Dawley rats (body weight $200{\pm}5g$) were divided into two groups; diabetic and nondiabetic groups. The groups were each randomly divided into two subgroups; fed control and coffee (5 g coffee powder/kg diet) diets. Diabetes was induced by intramuscular injection of 50 mg streptozotocin/kg body weight. Rats with blood glucose concentrations ${\geq}300mg/dL$ were considered diabetic for these experiments. All rats were fed an experimental diet and deionized water ad libitum for 4 weeks. Results: The results of this study indicate that body weight gain was significantly lower in diabetic groups than in nondiabetic groups regardless of diet. Mean food intake was significantly higher in diabetic groups than in nondiabetic groups, and significantly higher in the coffee group than in the control group in diabetic rats. Food efficiency ratio (FER) was significantly lower in diabetic groups than in nondiabetic groups regardless of diet. The fasting blood glucose of coffee supplemented groups was significantly lower compared with the control group in diabetic and nondiabetic rats. The levels of serum LDL-cholesterol and atherogenic index were significantly lower in the coffee group than in the control group in diabetic and nondiabetic rats, and serum HDL-cholesterol was significantly higher in the coffee group than in control groups. The contents of hepatic triglyceride were significantly lower in the coffee group than in the control group in diabetic and nondiabetic rats. The lipid peroxidation of malondialdehyde (MDA) contents was significantly lower in the coffee group than in the control group in diabetic and nondiabetic rats. Activity of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase in liver was not significantly different by experimental diets among all groups. Conclusion: In conclusion, effects of 0.5% coffee powder supplemented diet were beneficial on blood glucose and lipids in diabetic rats.

• Toxicological Research
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• v.20 no.3
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• pp.187-193
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• 2004

This study aimed to determine the association of maternal oxidative stress and adverse pregnancy outcome with serum vitamin C concentration and a myeloperoxidase (MPO) genetic polymorphism during pregnancy. We investigated 450 pregnant women who visited Ewha Womans University Hospital for prenatal care during gestational weeks 24～28. During the second trimester, we measured serum vitamin C levels and urinary 8-hydroxyde-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) as an oxidative stress biomarker. We determined the presence of a maternal MPO polymorphism (G-to-A substitution at nucleotide 463) using a PCR-RFLP assay. We compared the level of oxidative stress and birth weight with the vitamin C concentration and the presence of the MPO polymorphism. The mean level of maternal oxidative stress tended to be higher and the birth weight lower for MPO type A/A than for types A/G and G/G. Vitamin C levels above the 75 percentiles were associated with reduced concentrations of urinary MDA and 8-OHdG but increased birth weight. Our data demonstrate that oxidative stress and neonatal birth weight are associated with the MPO genetic polymorphism, with the association modified by the maternal vita-min C levels.

• Korean Journal of Community Nutrition
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• v.11 no.4
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• pp.479-487
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• 2006

Many epidemiological and observational studies show that distorted body image of normal body weight is widespread among Korean young females. This study was performed to evaluate the prevalence of overweight and underweight of young females and, to compare nutrient intakes, body composition, serum indices (lipids, MDA: Malondialdehyde, TAS : Total Antioxidant Status) with different BMI groups. The subjects were 75 university students in Seoul. We divided the subjects into 3 groups according to their BMI by IOTF guideline (UW: underweight group, BMI < 18.5, NW: normal body weight group; 18.5 $\leq$ BMI < 23.0, OW: overweight group; BMI $\geq$ 23.0). Data on dietary intakes, body compositions and serum indices were obtained in 3 groups. Differences on all of the above variables were assessed by body weight groups. Using IOTF guidelines, the prevalence of overweight and underweight in young females were 23%, 61%, 16% respectively. $\beta$-carotene and vitamin A intake of UW were significantly higher than that of NW (p < 0.05). Fiber intakes of NW was significantly higher than that of W (p < 0.05). But intakes of energy and the other nutrients were not significantly different among BMI groups. Calcium and folate intakes were 75%, 61% of KDRIs. Serum TGs were significantly higher in OW than that of NW, UW (p < 0.05), but level of MDA and TAS were not significantly different. The association of overweight and low intake of $\beta$-carotene and vitamin A may be one of many factors predisposing obese females to a high risk of oxidative stress later in life. This requires urgent nutritional intervention programs involving enough intake of fruit and vegetables, with modification of inappropriate dietary habits.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.8
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• pp.1310-1317
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• 2003

Effects of dietary cholesterol and/or taurine supplementation on plasma and hepatic lipid peroxidation status and antioxidant enzyme activities were evaluated in rats fed one of the following semisynthetic diets for 5 weeks: control diet (CD, cholesterol-free and taurine-free diet); high cholesterol diet (HCD, CD+1.5% cholesterol): high taurine diet (HTD, CD+1.5% taurine): high cholesterol and high taurine diet (HCHTD, HCD + 1.5% taurine). Plasma malondialdehyde (MDA) level was not influenced by dietary cholesterol or taurine supplementation, while hepatic MDA level was 70% higher in rats fed HCD compared to the value for CD rats (p<0.05). Our observation that taurine supplementation significantly decreased the hepatic MDA level of rats fed HCD, but failed to decrease lipid peroxidation of rats fed CD indicates that the protective effect of taurine in the liver against lipid peroxidation is manifested only under the hypercholesterolemic environment. Plasma and hepatic glutathione peroxidase (GSH-Px) activities were not affected by dietary supplementation of cholesterol or taurine. However, hepatic superoxide dismutase (SOD) activity was significantly reduced by dietary taurine supplementation (p <0.05), and thus significantly lower in rats fed HTD compared to the value for CD (p<0.05). Plasma total cholesterol concentration was positively correlated with hepatic cholesterol concentration as expected (r=0.712, p<0.001). Plasma (r=0.399, p<0.05) and hepatic cholesterol levels (r=0.429, p<0.05) showed a significantly positive correlation with hepatic MDA concentration, respectively. Plasma taurine concentration was negatively correlated with hepatic SOD activity (r=-0.481, p<0.01), and tended to be negatively correlated with hepatic GSH-Px activity without showing statistical significance (r=-0.188, p<0.05). These results indicate an antioxidative effect of taurine in rats with elevated level of lipid Peroxidation due to high intake of dietary cholesterol. Future application of taurine as a safe candidate for a hypolipidemic agent without adversely affecting body's antioxidant defense system is speculated.

• Korean Journal of Food Science and Technology
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• v.38 no.1
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• pp.99-103
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• 2006

Hepatoprotective effects of Bulro Kugi (Lycium chinense Mill) fruit extracts on $CCl_4-administered$ rats were investigated in vivo. Administration of $CCl_4$ increased plasma glutamic oxalacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and lactate dehydrogenase (LDH) activities, induced lipid peroxidation as measured by malondialdehyde (MDA) content of rat liver, and significantly increased liver weight. Feeding of B. Kugi (Lycium chinense Mill) slightly increased body weight gain, although not significantly different from normal group. B. Kugi (Lycium chinense Mill) fruit extracts reduced blood cholesterol level and inhibited $CCl_4-induced$ increases of plasma GPT, GOT, and LDH activities, whereas increased contents of MDA and cytochrome P-450, and GST activity in liver tissue of $CCl_4-administered$ rats. Roasted B. Kugi (Lycium chinense Mill) fruit extract showed highest hepatoprotective effect among samples tested. These results suggest water extracts of B. Kugi (Lycium chinense Mill) fruit possess promising hepatoprotective activity against $CCl_4-induced$ hepatic damage in rats.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.10
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• pp.1251-1257
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• 2008

For the first 42 days, we made rats obese by feeding them potato starch instead of corn starch and after that we fed them transformed potato starch by 4 groups for 70 days. The 4 groups are GPS group, SPS group, EZ group and H40 groups and each were fed normal potato, small potato, enzyme treated potato, and acid treated potato starches, respectively. We determined body weight and feeding efficiency, lipid profiles in serum, lipid peroxidation in tissues and redox antioxidant system as GSH and GP-x in vivo. As a result, there was no difference in the increment of body weight in the groups of GPS, EZ and H40. Therefore EZ group showed lower body weight increment than other groups. While GPS group and SPS group did not show significant difference in blood glucose, cholesterol level, LDL-cholesterol and TC, and their measured values were lower than those of EZ and H40 groups. No significant difference was found in HDL-cholesterol level except for GPS group. Furthermore, when calculating atherogenic index (AI) by HDL-cholesterol and TC contents, H40 group showed higher measured value than other groups. When measuring the lipid peroxidation in serum, kidney and liver tissues, the serum lipid peroxidation in H40 group was higher than others. In the tissue of liver and kidney, EZ and H40 groups showed significantly lower contents than others. The content of GSH showed different tendency in each tissue, but the measured value of GP-x activity was lower in SPS group.

• Korean Journal of Plant Resources
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• v.22 no.4
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• pp.330-336
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• 2009

The purpose of this study is to know the effect of wood vinegar ingestion on antioxidative defense system in trained rats. Twenty-eight male Sprague-Dawley male rats were used as the subject. Experimental groups were divided into CON-SED(control + sedentary), CON-EX(control + exercise), WV-SED(wood vinegar ingestion + sedentary), and WV-EX(wood vinegar ingestion + exercise), respectively. Body weight and stored fat weight(mesentery, retroperitoneal and epididymal) were not significantly differed in each group. Concentration of TG and FFA was not differed by wood vinegar ingestion. Concentration of glucose was revealed significant difference in exercise group rather than sedentary group, but there was no difference by wood vinegar ingestion between the groups. Contents of SOD in WV-EX group was significantly higher than other three groups. Contents of MDA in CON-EX group was significantly lower than CON-SED group, while the difference between CON group and WV group was not observed. In conclusion, wood vinegar ingestion with exercise seems to have a positive beneficial effects on reducing oxidative stress.

• Journal of Life Science
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• v.16 no.7 s.80
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• pp.1090-1096
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• 2006

The purpose of this study was to investigate the effects of p. Linteus and C. militaris supplement on lactate, phosphorous, ammonia, MDA(malondialdehyde), GPX(glutathione peroxidase) and TAS(total antioxidant status) following $VO_{2max}$, and 85% $VO_{2max}$ exercise. The 15 male college students were divided into three groups: 5 P. liteus supplied(PL), 5 C. militaris supplied(CM), and 5 placebos supplied(PB) and compared the differences between supplement before and after. Obtained results were as follows: In the exercise performance time, there were no differences in PL, CM and PB groups. Regular supplement of P. linteus or C. militaris partially reduces fatigue induction factors. Also, P. linteus or C. militaris supplement decreases MDA, increases GPX and TAS. Therefore, it can be concluded that P. linteus or C. militaris supplement not only have a pharmacological effect for clinical treatment, but also have a maintenance effects on the tissue oxidant-antioxidant system after exercise.

• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.150-160
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• 2012

The object of this study was to evaluate the effects of dietary supplementation of mulberry leaves and silkworm excreta ethanol extracts on weight performances, blood characteristics, cecal microflorae of chickens. Two hundred forty male broiler chicks(Ross) were fed diets for five weeks containing 0.1%(MLA) and 1%(MLB) of mulberry leaves ethanol extracts, and 0.1%(SEA) and 1%(SEB) of silkworm excreta ethanol extracts. Weight performance did show no significant difference in all test groups which were fed with supplementation of mulberry leaves and silkworm excreta ethanol extracts. They showed better weight gain and feed conversion than the negative control group which was fed only with forage without antibiotics. ABTS(2'-azine-bis[3-ethylbenzothiazoline-6-sulfonic acid]) test was conducted to investigate free radical scavenging activity of blood in tested groups. ABTS scavenging activities of tested groups were higher than control groups in significant level, though there was no significant difference(P = 0.396). Specifically, MLB group showed the highest scavenging activity. Blood-level concentration of MDA, which is an indicator of lipid peroxidation, was also decreased in tested groups and the lowest level was observed in SEA(P = 0.001). As storage time increased at $4^{\circ}C$, muscle-level MDA concentrations of all tested groups were generally increased and significant difference was obsereved between tested groups and controls in total increase of MDA concentration($P=4.417{\times}10^{-3}$). In cecal microflorae, SEA and SEB showed decreased total microbe population compared to NC($P=6.462{\times}10^{-5}$) and even to PC. Supplementation of mulberry leave and silkworm excreta ethanol extract did show a similar inhibition effect against Salmonella sp., furthermore, MLB did enhanced the growth of Lactobacillus sp.($P=3.636{\times}10^{-7}$). In summary, ethanol extract of silkworm excreta may be a potential alternative of antibiotics for chicks. In addition, both of ethanol extracts supplementation to broiler chicks would be very useful not only to improve antioxidant effect of blood but also to suppress lipid peroxidation without any loss of weight performance in poultry farming.

• Korean Journal of Food Science and Technology
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• v.29 no.1
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• pp.167-172
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• 1997

Effect of combined preparation (DWP715) containing Alaska pollack extract, maltol, ascorbic acid and nicotinamide on decreasing of blood alcohol was evaluated in human blood. Treatment of DWP715 prior to administration of 25% alcohol (100 mL) decreased alcohol concentration in blood and showed significant difference after 2 hours. The pharmacokinetic parameters such as area under the concentration-time curve (AUC), $C_{max},\;T_{max}\;and\;T_{1/2}$ were also decreased and delayed when compared with control values. Effects of DWP715 on anti-fatigue and anti-oxidation activities were also studied in the restraint stress model using various parameters (GOT, GPT, LDH values and organ weights) on mild condition and examined through the content of lipid peroxide induced by 2% $CCl_4$ in mouse livers. While GPT level, thymus and adrenal weight were not influenced by DWP715 dosing, LDH, GOT level and spleen weight used as a parameter against fatigue and stress states were recovered almost to the nomal level. Furthermore, lipid peroxidation due to $CCl_4$ was significantly inhibited by DWP715 treatment. These results suggest that DWP715 seems to metabolize the blood alcohol rapidly and to restore the damaged liver and fatigue conditions which was caused by alcohol metabolism to normal condition.