• Korean Journal of Environmental Biology
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• v.37 no.4
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• pp.690-706
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• 2019

Paddy fields play an important role in the biodiversity conservation of agroecosystems as man-made wetlands. This study focused on the floristic composition and characteristics of paddy fields in South Korea. The flora surveys were conducted in 33 areas in nine provinces at two times (June and August) in 2016. The results showed that the vascular plants of the paddy fields in South Korea included 389 taxa, composed of 51 families, 219 genera, 350 species, four subspecies, 30 varieties, and five forms. Among the 51 families, Gramineae was the most diverse in species (76 taxa), followed by Compositae (61 taxa), Leguminosae (32 taxa), Cyperaceae (27 taxa), Polygonaceae (15 taxa), and Scrophulariaceae (12 taxa). Based on the occurrence frequency of each species, Digitaria ciliaris (Retz.) Koel. (100%), and Echinochloa crus-galli (L.) P.Beauv. (100%) were the highest, followed by Acalypha australis L. (97.0%), Bidens frondosa L. (97.0%), Alopecurus aequalis Sobol. (97.0%), Rorippa palustris (Leyss.) Besser (93.9%), Ludwigia prostrata Roxb. (93.9%), Lemna perpusilla Torr. (93.9%), Mazus pumilus (Burm. f.) Steenis(90.9%), Rorippa palustris(Leyss.) Besser(87.9%), Eclipta prostrata(L.) L.(87.9%), Commelina communis L.(87.9%), Artemisia princeps Pamp.(84.8%), Erigeron annuus (L.) Pers.(84.8%), Cyperus microiria Steud.(84.8%), and Aneilema keisak(Hassk.) Hand.-Mazz. (81.8%). The biological type of paddy fields in South Korea was determined to be Th-R₅-D₄-t type. Rare plants were found in six taxa: Aristolochina contorta Bunge, Penthorum chinense Pursh, Sagittaria sagittifolia subsp. leucopetala (Miq.) Hartog, Ottelia alismoides (L.) Pers., Pseudoraphis ukishiba Ohwi, and Acorus calamus L. Eighty taxa contained naturalized plants composed of 15 families, 54 genera, 76 species, three varieties, and one form. The urbanization and naturalization indices were 24.8% and 20.6%, respectively.

• Weed & Turfgrass Science
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• v.5 no.2
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• pp.71-81
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• 2016

This study was conducted to investigate the weed occurrence and distribution on the orchard fields (apple, pear, peach, grape) at 387 sites in Chungbuk province of Korea from May to September in 2015. From the result of this survey, 200 weed species in 47 families were identified and classified to 82 annuals, 40 biennials and 78 perennials. Based on the occurrence ratio, the most dominant weed species in Chungbuk province orchard fields were higher in order of Digitaria ciliaris (4.12%), Echinochloa crus-galli (3.60%), Stellaria aquatica (3.45%), Artemisia princeps (3.07%) and Chenopodium album (3.06%). The composition of major occurred weed families, Compositae, Poaceae, Leguminosae and Cyperaceae were 21, 9, 6 and 6%, respectively. Coefficient of similarity between a various orchards based on the degree of dominance were ranged from 68.7-91.8%. The most important weed species at apple, peach, grape was Digitaria ciliaris while these for pear was Poa annua. Fifty-one exotic weed species were also identified. The results of this study could be useful information for estimation of future weed occurrence, weed population dynamics and establishment of weed control methods on the orchard fields in Chungbuk province of Korea.

• Weed & Turfgrass Science
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• v.4 no.4
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• pp.295-307
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• 2015

Surveys of weed species in paddy fields were conducted to identify weed occurrence on July 2013. Total 237 sites of paddy fields in 9 City/Gun, Jeonnam Provinces in Korea were investigated. From the survey, 45 weed species in 18 families were identified and classified to 33 annuals and 15 perennials. Based on the occurrence ratio, the most weed species belonged to Poaceae (9 species) and 8 and 4 weed species belonged to Cyperaceae and Scrophulariaceae, respectively. These 21 weed species in the most four families accounted for 46% of total weed occurrence. The most dominant weed species in Korean paddy fields were Echinochloa spp. (18.5%), followed by Monochoria vaginalis (12.3%), Lemna perpusilla (8.2), Eleocharis kuroguwai (5.2%) and Scirpus juncoides (5.2%). The most dominant weed species in machine transplanting paddy fields were Echinochloa spp. (14%), followed by Monochoria vaginalis (12%) and in water seeded rice paddy fields were Echinochloa spp. (25%), followed by Monochoria vaginalis (11%). This information could be useful for estimation of future weed occurrence, weed population dynamics and establishment of weed control methods in paddy fields.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.86-86
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• 2019

현삼과(Scrophulariaceae)에 속하는 긴산꼬리풀(Veronica longifolia L.)은 벽자색의 긴 꽃이 총상화서로 달려 청량감을 자아내어 분화소재로 이용한다면 단조로운 실내의 분위기를 환기할 수 있다. 따라서, 조경 및 관상용 식재에 용이한 긴산꼬리풀의 재배는 2018년 5월 15일에 파종하여 생산된 유묘를 2018년 7월 16일에 정식하여 2018년 10월 15일까지 수행하였다. 기비실험은 코트비료(싱싱코트)를 포트당 0, 10 및 20알 처리하였다. 추비는 hyponex (N-P-K, 4-6-6)를 4주 간격으로 총 3회 각 0, 1000 및 $2000mg{\cdot}L-1$로 엽면시비 하였다. 광량 실험은 무차광, 55 및 75% 차광막을 설치하여 수행하였다. 왜화제 종류 및 농도 실험은 dinizonazole(빈나리)과 daminozide (B-9)를 각 0, 1000 및 $2000mg{\cdot}L-1$ 농도로 처리하였으며, 적심처리는 4주차에 1회 처리 유무를 달리하였다. 모든 실험의 공통사항은 플라스틱 10호 화분에 원예상토를 충진한 후 200구 트레이에 종자를 셀당 4립씩 파종하여 생산된 유묘를 정식하였다. 연구의 결과, 기비처리는 초장, 마디수, 엽수가 증가하고, 절간장 및 측지수를 감소시켰으나, 유의적인 차이는 나타나지 않았다. 추비 처리는 농도가 높을수록 엽수가 증가하는 경향을 보였으며, $2000mg{\cdot}L-1$에서 무처리에 비해 유의적으로 많았다. 차광정도에 따른 긴산꼬리풀의 생육은 무차광 조건에서 생육이 가장 양호하였다. 차광률이 증가할수록 전반적인 생육이 억제되고, 개화율도 크게 감소하는 결과를 보였다. 왜화제 처리별로는 종류에 관계없이 전반적인 생육이 억제 되었다. Diniconazole 및 daminozide의 농도가 높을수록 초장이 억제되는 경향을 보였으나, 측지수, 마디수, 엽수도 감소하여 관상적 이용에 적합하지 않다. 적심을 할 경우 전반적인 생육은 큰 차이가 나타나지 않았으며, 측지수가 감소하였다. 따라서, 긴산꼬리풀의 분화생산을 위한 재배기술은 기비는 처리하지 않고, 추비는 $2000mg{\cdot}L-1$로 시비하며, 광이 충분한 곳에서 재배하는 것이 효과적이다. 또한, 왜화제와 적심은 일반적으로 줄기신장을 억제하고 마디수와 엽수를 증가시켜 관상적 이용효과가 있는 것으로 알려져 있지만 본 연구에서는 반대의 결과를 보여 처리 하지 않는 것이 효과적인 것으로 판단된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.88-88
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• 2019

현삼과(Scrophulariaceae)의 둥근산꼬리풀(Veronica rotunda Nakai)은 흰색의 꽃이 총상화서로 피며, 관상용으로 쓰인다. 또한, 접선골도라 하여, 전초를 약용하고 활혈, 지혈, 청폐열, 화간위에 효능이 있는 자생식물이다. 본 연구는 둥근산꼬리풀의 포트 재배방법 확립을 위하여 2018년 5월 15일에 파종하여 생산된 유묘를 7월 16일에 정식하여 10월 15일까지 약 14주 동안 수행하였다. 식재용기 실험은 비닐포트[3치($7{\times}7cm$), 4치($9{\times}8.5cm$)]와 플라스틱 화분[8호($7{\times}7cm$), 10호($9{\times}9cm$), 12호($10.5{\times}10.5cm$)]를 이용하였고, 토양 종류[중화 피트모스:펄라이트(3:1, 4:1), 마사토:중화 피트모스(2:1, 3:1)], 묘의 소질[파종 립수(1, 2, 4, 6립), 파종 용기(162, 200, 288구 트레이)] 등의 연구를 진행하였다. 대조구는 플라스틱 화분 10호에 원예상토를 충진하고 200구 트레이에 4립씩 파종하여 생산된 1셀을 정식하였다. 연구의 결과, 식재용기에 따른 둥근산꼬리풀의 생육은 플라스틱 12호 화분에서 가장 양호하였으며, 토양의 양이 많을수록 전반적인 생육이 증가하였다. 토양 종류별로는 원예상토에서 재배한 처리구에서 양호한 생육을 보였다. 중화 피트모스와 펄라이트 3:1 혼용토의 경우, 원예상토와 전반적인 생육에 큰 차이가 없어 이용가치가 있을 것으로 판단된다. 한편, 피트모스의 비율이 높은 4:1 혼용토에서는 비교적 생육이 억제되었으며, 잎이 황화되었다. 마사토와 중화 피트모스의 혼용토는 원예상토에 비해 왜화되는 결과를 보였으며, 측지수, 마디수, 엽수에는 큰 영향을 주지 않았다. 그러나 개화율을 감소시키는 결과를 보였다. 셀당 파종한 종자의 양을 달리하여 생산된 유묘를 정식하여 재배한 결과, 전반적인 생육은 1립 파종구에서 양호하였다. 한편, 파종량이 많을수록 초장, 절간장이 감소하며, 포트당 마디수, 측지수 및 개화율이 증가하여, 본 연구의 목표인 관상적 이용에 적합한 것으로 판단된다. 파종용기별 생산된 유묘이식 결과, 초기생육이 왕성할수록 후기생육도 우수하였다. 162구 생산묘 정식구는 다른 처리구보다 전반적으로 생육이 왕성하였으며, 다음으로 200구 순이었다. 파종용기에 따른 묘의 크기는 정식 후의 개화율에 유의적인 차이가 없었다. 따라서, 둥근산꼬리풀의 재배는 플라스틱 화분 12호에 원예상토를 충진하여 162구 트레이에 셀당 6립 파종하여 생산된 유묘를 정식하는 것이 분화용 재배에 효과적인 것으로 생각된다. 한편, 재배에 이용되는 토양의 종류에 따른 비율 및 개화촉진 등의 추가적인 연구가 필요하다.

• Korean Chemical Engineering Research
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• v.56 no.6
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• pp.832-840
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• 2018

In this study, both of the concentration of benzo(a)pyrene in 5 species (total 50 samples) of medicinal herbs and their transfer ratios in the preparation steps of water extract(decoction) and soft extract, were measured by HPLC/FLD. The calibration curve of benzo(a)pyrene shows excellent correlation over the concentration range of 3~40 ng/mL with the correlation coefficient ($R^2$) of 1.000. The detected benzo(a)pyrene concentrations from the medicinal herbs ranged from non-detection to $37.54{\mu}g/kg$, and their average was $6.73{\mu}g/kg$. Among the total samples, 15 samples (i.e., 30%) exceeded the limit of herbal medicine benzo(a)pyrene criteria (i.e., $5{\mu}g/kg$) according to the notification No. 2009-302 from Ministry of Food and Drug Safety. In particular, the concentration of benzo(a)pyrene in Coptidis Rhizome was turned out to be the highest of $37.54{\mu}g/kg$. The detected benzo(a)pyrene concentrations from water extract(decoction), soft extract and remnant after boiling, ranged from non-detection to $2.31{\mu}g/kg$, non-detection to $2.28{\mu}g/kg$, and 2.18 to $21.91{\mu}g/kg$, respectively. In preparation of water extract(decoction) and soft extract, transferred benzo(a)pyrene was not detected or, if transferred, the maximal transfer ratios of benzo(a)pyrene were shown to be 8.9% and 9.8%, respectively. Therefore, the content of benzo(a)pyrene in the samples of herbal medicine used in this study, were reduced by more than 90% in preparation steps of water extract (decoction) and soft extract.

• Korean Journal of Environment and Ecology
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• v.11 no.4
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• pp.407-414
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• 1998

The rare and endangered plants in Naesorak area were investigated for four months during May to August in 1997, and the results were summarized as 35 taxa which is classified as 19 families, 32 species, and 3 varieties. At the level of families, 19 families such as Pinaceae(1 species), Taxaceae(1 species), Cupressaceae(1 species and 1 variety), Liliaceae(5 species and 1 variety), Aristolochiaceae(2 species), Ranunculaceae(4 species), Berberidaceae(1 species), Saxifragaceae(2 species), Rosaceae(1 species), Oleaceae(2 species and 1 variety), Solanaceae(1 species), Scrophulariaceae(1 species), Valerianaceae(1 species), Campanulaceae(1species), and Compositae(1 species) were recorded. The most of the habitats of the species was easily found along the major mountain trails, and a kind of destructions of these plants by human interferences is due to the fact that the illegal collections for food and medicine are occurred. Those species of Taxus caespitosa, Rhododendron aureum, Vaccinium bracteatum, Allium victoralis var. platyphullum and Leontice microryncha has a few individuals in habitats and also shows the unfavorable growing state, so the intensive and continuous habitat management of the habitats were inevitably dimanded.

• Journal of agriculture & life science
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• v.50 no.5
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• pp.81-93
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• 2016

Rehmannia glutinosa(Gaertn.) DC. is a herbaceous perennial plant and belonging to the Scrophulariaceae and used as roots for medicinal part and purpose. R. glutinosa is and usually used for fresh rehmannia root or prepared rehmannia root. However, it is very difficult to propagate using the seeds because of lack germination so it is propagated using the vegetative method as the rootstock. Currently, propagation and harvesting using the rootstock of R. glutinosa has difficulties about production of the high quality and quantity in R. glutinosa because of root rot disease. To optimize in vitro cultures and to improve the rootstock and seedling of R. glutinosa after morphological and genetical determination, 5 plant culture media (MS, DJ, LS, QL, and WPM) were used in this study then WPM was selected for better growth, for multiplication condition(WPM + IAA 1.0 mg/L + IBA 0.5 mg/L), and for root enlargement condition(WPM + NAA 0.1 mg/L) of R. glutinosa. Based on these results, in vitro seedlings of R. glutinosa were transferred to soil for acclimation with environment adaptation and shown the positive effects about root enlargement and root formation. Therefore, it can be used for high quality of R. glutinosa production and production of the rootstock based on propagation using in vitro seedlings of R. glutinosa.

• Microbiology and Biotechnology Letters
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• v.36 no.3
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• pp.201-208
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• 2008

To produce bioactivity-strengthen medicinal herbs, the 36 medicinal herbs which have antioxidation or blood circulation activity, were solid fermented using Phellinus baumii mycelium. Most of medicinal herbs, except Chrysanthemum indicum (flower), Zizyphus jujuba Miller (fructus), Aconitum koreanum R. Raymond (root), Magnolia denu-data (flower), and Polygonatum sibiricum Redt (root bark), showed good fermentation at $25^{\circ}C$ for 20 days under 90% of relative humidity. The poor fermentations of the herbs could be explained by lack of nutrient, structural rigidity, and the content of antifungal substance. After fermentation, the average water content of herbs were increased to $67.21{\pm}11.43%$ from $30.84{\pm}15.67%$, but the average pH and average methanol extraction ratio were slightly decreased to $11.16{\pm}7.06%$ and $4.83{\pm}0.73$ from $13.91{\pm}12.22%$ and $5.06{\pm}0.87$, respectively. The analysis of thrombin inhibition and DPPH scavenging activity of the methanol extracts of herbs showed that thrombin inhibition activities of the fermented Drynaria fortunei Kunze, Melia azedarach var. japonica, Prunus persica and Orostachys japonicus, and DPPH scavenging activities of the fermented Polygala tenuifolia, Scrophularia buergeriana, Angelica dahurica, Drynariafortunei Kunze, Cyperus rotundus, and Boschniakia rossica were increased as compared with those activities of non-fermented its cognate herbs. Our results suggest that the production of bioactivity-strengthen medicinal herbs is possible by solid fermentation of Phellinus baumii mycelium, as fermented Drynaria fortunei Kunze showed increased antioxidant and thrombin inhibitory activities than those of non-fermented herbs.

• Journal of Life Science
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• v.20 no.8
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• pp.1268-1275
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• 2010

This study was carried out to investigate the anti-proliferative activity of solid-state fermented medicinal herbs which include Phellinus baumii. Methanol extracts were prepared from 36 different medicinal herbs and their fermented counterparts. These extracts were used to treat human colorectal HCT116 cell, human embryonic kidney cell HEK-293, pre-adipocyte cell 3T3-L1, and pre-osteoblast cell MC3T3-E1 for 24 hr. At a concentration of 100 ${\mu}g/ml$, the extracts of Amomum villosum, Cnidium officinale Makino, Dendrobium moniliforme, Dictamnus dasycarpus, Diospyros kaki Thunb, Eucommia ulmoides Oliv, Ginkgo biloba L, Magnolia denudata Desrousseaux, Orostachys japonicus, Panax notoginseng, Pharbitis nil Choisy, Polygala tenuifolia and Trichosanthes kirilowii (seed) led to a < 50% decrease in cell proliferation, and mycelium of P. baumii showed a 46.3% decrease in cell proliferation. Meanwhile, the extracts of the 25 fermented herbs showed similar anti-proliferative activities compared to those of individual non-fermented herbs. However, the extracts of the fermented Drynaria fortunei Kunze (1), Lycium chinense Mill (2), Fritillaria thunbergii Miquel (3) and Prunus persica showed increased anti-proliferative activity. The $IC_{50}s$ of (1), (2) and (3) were especially decreased to 28, 85 and 80 ${\mu}g/ml$ from 394, 917 and 149 ${\mu}g/ml$, respectively. Furthermore, the cytotoxicity of the extracts of fermented (1), (2) and (3) against HEK-293, 3T3-L1, and MC3T3-E1was negligible up to 200 ${\mu}g/ml$. These results suggest that solid-state fermentation using the mycellium of P. baumiiproduce potential anti-cancer agents or strengthen the bioactivity of medicinal herbs.