• Title/Summary/Keyword: 핵다각체병 바이러스

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Structure of Spodoptera exigua Nucleopolyhedrovirus p10 Gene (파밤나방 핵다각체병 바이러스의 p10 유전자 구조)

  • 최재영;우수동;홍혜경;이해광;제연호;강석권
    • Korean journal of applied entomology
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    • v.38 no.2
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    • pp.145-149
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    • 1999
  • To develop the baculovirus expression vector system (BEVS) adopting p10 gene promoter of Spodoptera exigua nucleopolyhedrovirus (SeNPV), we characterized the p10 gene of SeNPV. The nucleotide sequence of 545 bases including the coding region of p10 gene was determined. Compared with the previously reported SeNPV p10 gene (Zuidema et al., 1993), 4 bases were different in the 5' and 3' flanking region but no difference was found in the coding region. The p10 gene was located within Xho I 1.5 Kb, Sph 1 2.4 Kb and Cla I 4.0 Kb fragments by Southern hybridization analysis. Also, the Sph I 2.4 Kb and the Cla I 4.0 Kb fragments were cloned and their restriction enzyme maps were determined.

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Effect of Nuclear Polyhedrosis Virus and NeemAzal-T/S on Spodoptera litura (Lepidoptera: Noctuidae) (담배거세미나방(Spodoptera litura)에 대한 핵다각체병바이러스와 NeemAzal-T/S의 혼합 살포효과)

  • 김선곤;김도익;박종대;박인진;임대준;김규진
    • Korean journal of applied entomology
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    • v.40 no.2
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    • pp.137-141
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    • 2001
  • This experiment was conducted to investigate the control effect of nuclear polyhedrosis virus and NeemAzal-T/S on Spodoptera litura larvae. In laboratory test, values of$ LT_{50}$ and $LT_{95}$ when treated with S. litura nuclear polyhedrosis virus (SINPV) $1$\times$10^{8}$ PIBs/ml plus NeemAzal-T/S 200 ppm were 1.94 and 8.33 days, respectively. Control effect of the combination of SINPV $1$\times$10^{8}$ plus NeemAzal-T/S 200 ppm was higher than the other concentrations. This mixed treatment could reduce LT$_{95}$ by 3 days. When SINPV alone was sprayed to the S. litura larvae reared on chinese cabbage seedling, the mortalities were 10.7~6.7% at 4 days after treatment. In combinations of SINPV plus NeemAzal-T/S at each level of concentration, the mortalities appeared faster and higher at 4 days after application than single treatment. Especially, the mortalities by combinations of SINPV $1$\times$10^{8}$ /PIBs/ml plus NeemAzal-T/S at 75~200 ppm were 100% at 9 days after treatment. The body weight of untreated larvae was increased 9.4-folds from 235 mg to 2194 mg after 7 days. However, the increasing levels of larval weight were 4.8- and 7.0-folds in the separate treatments of NeemAzal-T/S and SINPV, respectively. Whereas in the combinations of SINPV $10^{4~8}$ PIBs/ml plus NeemAzal-T/S 75~200 ppm, larval weight was increased 3.9 to 2.9-folds. These results showed that the mortality and inhibition of larval weight in the combination of SINPV and NeemAzal-T/S were highly enforced by synergistic effect.

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Pathogenicity and Production of Spodopetra exigua Nuclear Polyhedrosis Virus (파밤나방 핵다각체병 바이러스의 병원성 및 증식)

  • 최재영;김혜성;진병래;설광열;박호용;강석권
    • Korean journal of applied entomology
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    • v.35 no.3
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    • pp.228-231
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    • 1996
  • To produce Spodoptera exigua nuclear polyhedrosis virus (SeNPV) using S. exigua larvae, the efficiency of the in vivo production was analysed by larval instar, inoculum and mortality. The results revealed that the mortality of 4th instar larvae inoculated with 1.OX 10' EIBs per ml was 86.7% and the yields of SeNPV was maximal, demonstrating that 4th instar larvae inoculated with 1 . 0l~o6 PIBs per ml were effective to mass production of SeNPV.

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Application of Neo-PPS Fumigation to the Disinfection of the Silkworm Larvae, Bombyx mori(L.), for the Control of Silkworm Diseases(I) Effect of Neo-PPS Fumigation on the Virus Diseases (Neo-PPS 훈증에 의한 잠체 소독에 관한 연구(I) -누에 바이러스병에 대한 약효-)

  • 임종성;김근영
    • Journal of Sericultural and Entomological Science
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    • v.18 no.2
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    • pp.79-81
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    • 1976
  • Experiments on the disinfection of the silkworm larvae by the application of Neo-PPS fumigation have been carried out for the control of virus diseases. The results obtained are summarized as follows. The disinfection of 2, 4 and 6 hours'fumigation with Neo-PPS (para-formaldehyde) showed an outstanding effect on the inactivation of the both viruses, nuclear polyhedrosis virus and cytoplasmic polyhedrosis virus, without significance in the 2. 4. and 6 hours' treatment.

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Genomic Recombination of Bombyx mori and Autographa californica Nuclear Polyhedrosis Viruses (누에 및 Autographa californica 핵다각체병 바이러스에 대한 유전자 재조명)

  • 우수동;박범석;박지현;정인식;양재명;강석권
    • Korean journal of applied entomology
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    • v.32 no.4
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    • pp.407-413
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    • 1993
  • Twelve recombinant viruses with wider host range were plaque purified after coinfectian of Autographa cahjornica and Bombyx mOT! NPVs into Sf9 ar BmN-4 cells. Restriction endonucleases analysis of the recombinant's DNAs showed that the recombinatIOn between AcNPV and BmNPV genomes had occurred more than once. When the recombinam RecB-8, derived from BrnN-4 cells, was observed by electron rntcroscopy, the shape of the polyhedron was a regular tetrahedron, and few virions were occluded into a polyhedron.

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Pathogenicity of Nuclear Polythedrosis Virus Isolated from the Tobacco Cutworm, Spodoptera litura (담배거세미나방 핵다각체병 바이러스의 병원성)

  • 임대중;박범석;진병래;최궤문;강석권
    • Korean journal of applied entomology
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    • v.27 no.4
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    • pp.219-224
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    • 1988
  • Rearing of Spodoptera litura fed on arfificial diet basically formulated with kindby bean powder was much promisible for production of S. litura nuclear polyhedrosis virus(SINPV) than that on kidney bean leaves. The $LC_{50}$ of SINPV against 3rd and 5th instar were $4.48{\times}10^{3}$ PIB/ml and $4.52{\times}10^{4}$ PIB/ml, respectively. The $LC_{50}${/TEX} of SINPV varied from 5.8 days to 7.7 days at higher inocula in the testes. The larval growth of 1st and 3rd instar larvae fed on virus were much delayed and the larval were killed within 4th instar in the former and within 5th instar in latter. S. litura larvae were very susceptible to multinucleocapsid nuclear polyhedsosis virus (MNPV) of Autographa californica, S. littoralis and Trichoplusia ni.

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Experiment of the formulation for the viral pesticide of nuclear polyhedrosis virus of the fall webworm, Hyphantria cunea Drury. (흰불나방 핵다각체병 바이러스의 제제화에 관한 시험)

  • Jin, Byeong-Rae;Kim, Gwon-Yeong;Gang, Seok-Gwon
    • Journal of Sericultural and Entomological Science
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    • v.29 no.2
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    • pp.51-57
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    • 1987
  • The intent of this research is to acquire some basic informations about formulation of the viral pesticide, Hyphantria cunea nuclear polyhedrosis virus and its virulence under field condition. The nuclear polyhedrosis virus was formulated as wettable powder using spreader, sticker and U.V. protector. The formulated product and aqueous virus were diluted with water at the concentration of 1${\times}$106PIB/ml and sprayed on mulberry leaves in the field. The leaves were fed with 3rd instar larvae of H. cunea to determine the inactivation period of the viral pesticides. The aqueous virus was completely inactivated on 5th day after spray, while the formulated one showed a spare mortality to the larvae even on 20th day after spray. In field application test, The fromulated and aqueous virus were sprayed on individual mulberry tree and 3rd instar laevae of H. cunea were fed on the trees. The mortality of the larvae one day after spray of the formulated and aqueous virus were about 50% and 40%, respectively. The formulated virus exhibited a persistent virulence to the larvae up to 9th day after spray, which the mortality was approximately 30%. The residual virulence of the formulated and aqueous virus was extended up to 4th day and 2nd day after spray, respectively.

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Characterization of Spodoptera exigua Nuclear Polyhedrosis Virus Polyhedrin Gene Structure (파밤나방 핵다각체병 바이러스의 다각체 단백질 유전자 구조)

  • 최재영;김우진
    • Journal of Sericultural and Entomological Science
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    • v.38 no.2
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    • pp.144-149
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    • 1996
  • To develope the baculovirus expression vector system (BEVS) using Spodoptera exigua nuclear polyhedrosis virus (SeNPV), we characterized the polyhedrin of SeNPV. The SeNPV polyhedra was irregular and composed of the major protein molecular weight of 30 kDa determined by electronmicroscopy and SDS-AGE analysis, respectively. The nucleotid suquences of 876 bases including the coding region of polyhedrin gene was determined and it was revealed that the polyhedrin gene is located within Xho I 3.0Kb and Nco I 6.0 Kb by Southern blot analysis, respectively. Also, the Xho I 3.0 Kb and the Nco I 6.0 Kb fragments were cloned and restriction enzyme map of these clones were determined.

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