• Title/Summary/Keyword: 해양 생산

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Structure Elucidation of a Potent Anti-MRSA Antibiotic, AM3, Produced by Streptomyces sp. (방선균에 의해 생산된 항 MRSA 항생물질 AM3의 구조 연구)

  • Lim, Yoong-Ho;Chang, Jun-Hwan;Kim, Jong-Hoon;Suh, Jung-Woo;Jung, Jae-Kyung;Lee, Chul-Hoon
    • Applied Biological Chemistry
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    • v.38 no.6
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    • pp.516-521
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    • 1995
  • In order to find a potent anti-methicillin resistant Staphylococcus aureus (MRSA) antibiotic, actinomycetes isolated from the samples collected in Korean marine silt were screened. From the culture broth of the isolated Streptamyces strain AM045, a substance showing excellent biological activity against MRSA was found, isolated and named AM3. The compound showed strong activities against MRSA, S. epidermidis, E. faecium and E. faecalis, which were better than those of vancomycin and teicoplanin. Unfortunately, AM3 was identified as Actinomycin V. However, this paper reports the three dimensional study of AM3 based on high resolution nmr and Computer Aided Molecular Modeling(CAMM), and the fact that the structure of the pentapeptide lactone ring with oxo-proline in chloroform solution does not have 'C conformation' any more.

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A Study on Establishment of Local Water Level Network in Sub-river Area of the coastal basin (해안유역 지방하천의 효율적 물관리를 위한 수위관측망 구축)

  • Jang, Bok-Jin;Kim, Jin-Hoon;HwangBo, Jong-Gu;Ryu, Young-Yong;Kim, Dae-Hee
    • Proceedings of the Korea Water Resources Association Conference
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    • 2012.05a
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    • pp.666-666
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    • 2012
  • 수문조사는 효율적이고 안정적인 물관리를 위한 기초자료로서 매우 중요함에도 불구하고, 예산 부족 등의 문제로 인해 국가하천 및 방재상 중요 하천유역을 중심으로 이루어졌다. 따라서 국내에서는 지방하천, 특히 해안유역 지방하천의 수문조사가 매우 미흡한 실정이다. 본 연구에서는 영산강서해와 섬진강남해 해안유역의 지방하천을 대상으로 도상 및 문헌을 통해 우선 현황을 파악하였으며, 이를 토대로 현장조사 등을 통해 수리 수문학적 중요성, 사회 경제적 분석과 수문조사 적정성을 검토하여 영산강서해유역에 총 7 개 지점, 섬진강남해유역에 총 13 개 지점에서 수위 및 유량 관측이 필요한 것으로 판단되었다. 이 중 12개 지점은 관측소 신설이 필요하며 이 지점들에 대해서는 수위관측시설의 설치 및 운영과 유량측정에 필요한 제반사항을 제시하여 향후 수위관측소 설치 및 유량측정 등에 활용할 수 있도록 하였다. 본 연구의 결과는 국토해양부 영산강홍수통제소 관할 해안유역에서의 균형적인 수문조사에 기여할 수 있을 것으로 기대되며, 향후 수위관측소 신설 후 생산되고 제공되는 수문자료들은 대상 해안유역에서의 효율적 물관리를 위한 중요 기초자료로서 충분히 활용될 것이다.

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Complete genome sequence of an indigo producing strain Yangia sp. TSBP01, isolated from oil-contaminated sediment (인디고 생산능이 있는 Yangia sp. TSBP01의 유전체 분석)

  • Kim, Hae-Seon;Cha, Sun Ho;Suk, Ho Young;Park, Nyun-Ho;Woo, Jung-Hee
    • Korean Journal of Microbiology
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    • v.54 no.3
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    • pp.293-294
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    • 2018
  • Yangia sp. TSBP01, isolated from tidal flat sediment contaminated by the oil spill, is known to convert indole to indigo via an intermediate called indoxyl. Our analysis revealed that Yangia sp. TSBP01 contained the genome of 5,165,974 bp (G + C content: 66.5%) being composed of two chromosomes and five plasmids. This strain had genes encoding several oxygenases such as indole oxygenase directly involved in the conversion of indole to indoxyl.

A Development and Characterization of Refractory Panel (내화패널의 개발과 특성에 관한 연구)

  • Kim, Dong-Joon;Kwon, Young-Jin;Kim, Yong-Ro;Han, Byung-Chan;Kim, Jae-Hwan
    • Proceedings of the Korea Institute of Fire Science and Engineering Conference
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    • 2009.04a
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    • pp.61-66
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    • 2009
  • 최근 초고층 건축물 등에 적용되는 고강도콘크리트의 내화성능에 대한 문제점이 제기됨에 따라 국토해양부에서는 고강도콘크리트 내화성능 관리 기준을 고시한 바 있으며, 건설업계에서도 이에 대응하기 위해 다양한 기술 검토가 이루어지고 있다. 본 연구에서는 고강도콘크리트의 취약점으로 제기되고 있는 화재시의 폭렬문제에 대한 대응방안으로서 기존 연구를 통해 내화성능이 우수한 것으로 보고되고 있는 ECC 를 영구거푸집으로 활용한 고강도콘크리트의 내화성능을 검토하였다. ECC 영구거푸집을 활용한 고강도콘크리트 기둥부재의 내화성능 검토 결과, 영구거푸집과 고강도콘크리트 계면으로의 열 침투를 제어할 수 있도록 부재 생산 및 구축 방안을 검토하고, ECC의 적정 배합 및 두께를 확보한다면 고강도콘크리트의 내화성능 확보기술로서의 활용이 가능할 것으로 판단된다. 또한, 내화성 영구거푸집으로서의 단순 활용 방안 이외에 ECC의 우수한 물리적 성능을 활용하여 구조성능을 분담할 수 있는 방안으로의 지속적 검토가 필요할 것으로 판단된다.

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Verification for applied water management technology of Global Seasonal forecasting system version 5 (확률장기예보GloSea5의 물관리 활용을 위한 검증)

  • Moon, Soojin;Hwang, Jin;Suh, Aesook;Eum, Hyungil
    • Proceedings of the Korea Water Resources Association Conference
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    • 2016.05a
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    • pp.236-236
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    • 2016
  • 현재 댐운영 계획 수립 시 매월 유지해야 하는 저수량의 범위를 나타낸 기준수위가 사용되고 있으며 매년 홍수기 말에 현재의 수문 상황과 장래의 전망을 통한 시기별 연간, 월간 댐운영 계획을 수립하고 있다. 물관리의 이수측면에서 댐수위 운영계획 수립과 홍수기 운영목표 수위를 결정하는데 활용하기 위해서는 계절단위, 연단위의 기상정보가 필요하다. 본 연구에서는 기상청에서 운영하고 제공하는 전지구 계절예측시스템 GloSea5(Global Seasonal forecasting system version 5)자료를 활용하여 금강유역에 적용하고자 하였다. GloSea5는 전지구계절예측시스템으로 대기(UM), 지면(JULES), 해양(NEMO), 해빙(CICE)모델이 서로 결합되어 하나의 시스템으로 구성되어 있으며 공간 수평해상도는 N216($0.83^{\circ}{\times}0.56^{\circ}$)으로 중위도에서 약60km이다. Hindcast자료는 유럽중기예보센터(ECMWF)에서 생산된 ERA-Interim 재분석장을 대기 모델의 초기장으로 사용하며 기간은 1996~2009년의 총 14년이다. 예보자료의 검증은 예보의 질을 결정하는 과정으로 Brier Skill Score (BSS), Reliability Diagrams, Relative Operating, Characteristics (ROC)등을 통해 정확성과 오차에 의한 예보의 성능을 검증하였다. 또한 Glosea5의 통계적 상세화를 수행하여 다양한 변수가 갖는 계통적인 지역 오차를 보정함으로써 자료의 신뢰도를 향상시키고자 하였으며 이는 이후 수문모델과의 연계 시 보다 정확하고 효율적인 댐운영에 활용할 수 있는 기후예측정보를 제공할 수 있을 것으로 판단된다.

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Performance Characteristics of a 50-kHz Split-beam Data Acquisition and Processing System (50 kHz Split Beam 데이터 수록 및 처리 시스템의 성능특성)

  • Lee, Dae-Jae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.5
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    • pp.798-807
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    • 2021
  • The directivity characteristics of acoustic transducers for conventional single-beam echo sounders considerably limit the detection of fish-size information in acoustic field surveys. To overcome this limitation, using the split-aperture technique to estimate the direction of arrival of single-echo signals from individual fish distributed within the sound beam represents the most reliable method for fish-size classification. For this purpose, we design and develop a split-beam data acquisition and processing system to obtain fish-size information in conjunction with a 50-kHz single-beam echo sounder. This split-beam data acquisition and processing system consists of a notebook PC, a field-programmable gate array board, an external single-transmitter module with a matching network, and four-channel receiver modules operating at a frequency of 50-kHz. The functionality of the developed split-beam data processor is tested and evaluated. Acoustic measurements in an experimental water tank showed that the developed data acquisition and processing system can be used as a fish-sizing echo sounder to estimate the size distribution of individual fish, although an external single-transmitter module with a matching network is required.

Phylogeny of Yeasts Isolated from the Flower of Aster spathulifolius Maxim. and Screening of Biosurfactant Producers (해국(Aster spathulifolius Maxim.)의 꽃에서 분리한 효모의 분자계통학적 분포해석 및 생물계면활성제 생산 균주의 스크리닝)

  • Kim, Jong-Shik;Kim, Dae-Shin
    • Korean Journal of Environmental Agriculture
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    • v.37 no.4
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    • pp.312-316
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    • 2018
  • BACKGROUND: Yeast biotechnology finds applications in various industries. Hence, we sought to explore the yeasts associated with the flower of Aster spathulifolius Maxim. This study aimed to isolate yeasts from the flower of the plant and screen for biosurfactant-producing yeasts. METHODS AND RESULTS: We collected flowers of Aster spathulifolius Maxim. and performed pure isolation using four types of media. In total, 117 strains belonging to 4 genera, namely, Cryptococcus (75 strains), Aureobasidium pullulans (30 strains), Candida (11 strains), and Rhodotorula (1 strain), were isolated and identified by ITS sequencing. Upon in-depth analysis, Cryptococcus, the most dominant genus (75 strains) was categorized into the 'Unknown group'. Upon in-depth analysis of A. pullulans, we discovered the 'Unknown group I' (27 strains) and the 'Unknown group II' (2 strains), which have not been reported previously. Two A. pullulans isolates with potent surfactant activity were selected via the screening procedure. CONCLUSION: In this study, a total of 117 strains were isolated from the flower of Aster spathulifolius Maxim. In addition, two biosurfactant-producing yeasts were identified from among the isolated yeasts.

Influence of Water Temperature on Air Temperature around Eastern and Western Coastal Areas of the Korean Peninsula during Winter (동계 한반도 동·서 연안역 기온에 미치는 수온의 영향)

  • Hong, Chul-Hoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.1
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    • pp.92-96
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    • 2019
  • The influence of water temperature (WT) on air temperature (AT) in the eastern and western coastal regions of the Korean peninsula in the winter was investigated using historical data from the Korean Meteorological Agency (KMA) and the National Institute of Fisheries Science (NIFS), focusing on the relationship between AT and WT. The data analysis shows that, during winter, the AT is generally higher by +1.9 to $+2.7^{\circ}C$ at Kangreung ($37.2^{\circ}N$) in the eastern region than at Inchon ($37.4^{\circ}N$) in the western region, i.e., the AT in the eastern region of the Korean Peninsula tends to be higher overall than that in the western region when similar latitudes are compared. On the other hand, in the winters of 1977-2006, the WT at Sokcho was higher by $+0.8^{\circ}C$ (January) to $+2.3^{\circ}C$ (March) than that at Sochungdo, directly resulting in increased AT ($+1.22^{\circ}C$) at Sokcho. This study suggests that higher AT in the eastern region during winter is caused by the influence of water flow, such as the East Korean Warm Current in the East/Japan Sea.

Production of monoclonal antibodies to immunoglobulin M of sevenband grouper (Epinephelus septemfasciatus) (능성어(Epinephelus septemfasciatus) immunoglobulin M에 대한 단클론 항체 생산)

  • Kim, Si-Woo;Kim, Jong-Oh;Kong, Kyoung-Hui;Oh, Myung-Joo;Kim, Wi-Sik
    • Journal of fish pathology
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    • v.34 no.1
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    • pp.111-115
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    • 2021
  • Immunoglobulin M (IgM) of sevenband grouper (Epinephelus septemfasciatus) was purified by mannan-binding protein (MBP) affinity column. The purified IgM had an apparent molecular weights of 76 (heavy chain) and 28 (light chain) kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Eight hybridoma clones secreting monoclonal antibodies (mAbs) against sevenband grouper IgM were established. Antibody detection enzyme-linked immunosorbent assay (ELISA) with bovine serum albumin (BSA, antigen) and the 8 mAbs revealed that optical density (OD) values were clearly different between sera from BSA-immunization and non-immunization of sevenband grouper. These results suggest that the produced mAbs in this study are specifically reacted with IgM of sevenband grouper.

Ecosystem-based Fishery Risk Assessment of Tuna Fisheries in the Western Indian Ocean (서부인도양 해역 다랑어어업의 생태계기반 어업 위험도 평가)

  • Young Shin Ha;Sung Il Lee;Youjung Kwon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.4
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    • pp.449-461
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    • 2023
  • The aim of this study was to conduct an ecosystem-based fishery risk assessment of tuna fisheries in the Western Indian Ocean. We selected gillnet, purse seine, hand line, baitboat, and longline fisheries as the target fisheries method, and selected longtail tuna (Thunnus tonggol), narrow-barred Spanish mackerel (Scomberomorus commerson), kawakawa (Euthynnus affinis), skipjack tuna (Katsuwonus pelamis), yellowfin tuna (T. albacares), bigeye tuna (T. obesus), albacore tuna (T. alalunga) and swordfish (Xiphias gladius) as the target species. The risk score for the size at the first capture in sustainability objective was high, especially, for the purse seine and baitboat fisheries using the fish aggregating devices (FADs). The risk score for the bycatch in the biodiversity objective was high for the gillnet fishery, and the gillnet fisheries using FADs showed high risks for the habitat quality objective due to the loss of the fishing gears. With regards to the socio-economic benefits objective, the risk score of the sales profits was low due to high sales of the tuna fisheries. The ecosystem risk score in the Western Indian Ocean was estimated to be moderate, although management is required for some of the indicators that have high-risk scores.