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http://dx.doi.org/10.7847/jfp.2021.34.1.111

Production of monoclonal antibodies to immunoglobulin M of sevenband grouper (Epinephelus septemfasciatus)  

Kim, Si-Woo (Department of Aqualife Medicine, Chonnam National University)
Kim, Jong-Oh (Institute of Marine Life Science, Pukyong National University)
Kong, Kyoung-Hui (Department of Aqualife Medicine, Chonnam National University)
Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University)
Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University)
Publication Information
Journal of fish pathology / v.34, no.1, 2021 , pp. 111-115 More about this Journal
Abstract
Immunoglobulin M (IgM) of sevenband grouper (Epinephelus septemfasciatus) was purified by mannan-binding protein (MBP) affinity column. The purified IgM had an apparent molecular weights of 76 (heavy chain) and 28 (light chain) kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Eight hybridoma clones secreting monoclonal antibodies (mAbs) against sevenband grouper IgM were established. Antibody detection enzyme-linked immunosorbent assay (ELISA) with bovine serum albumin (BSA, antigen) and the 8 mAbs revealed that optical density (OD) values were clearly different between sera from BSA-immunization and non-immunization of sevenband grouper. These results suggest that the produced mAbs in this study are specifically reacted with IgM of sevenband grouper.
Keywords
Immunoglobulin M; sevenband grouper; monoclonal antibody; mannan-binding protein affinity column;
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