• Journal of the Korean Society for Marine Environment & Energy
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• v.13 no.4
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• pp.249-262
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• 2010

Regulatory test method for Total Petroleum Hydrocarbons (TPHs) in the marine sediment and biota has not still been established even though TPHs are one of the major pollutants in marine environment. Based on the Korean Soil Standard Method (SSM) for TPHs, we considered a new treatment method for determining TPHs in marine environmental samples by using a Gas chromatography coupled with Mass spectrometric detector. We suggested an improved recovery test for quality control procedures and introduced analytical procedures of removing sulfur, polar organic materials, water and saponification for removing neutral lipids in marine bottom sediments and biota.

• Environmental Analysis Health and Toxicology
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• v.17 no.4
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• pp.341-346
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• 2002

To investigate the residue of organophosphorus pesticides such as Monocrotophos［Dimethyl-1-methyl-2-methyl carbamoylvinylphosphate］and EPN［O-ethyl-O-4-nitrophenyl phenylphosphonothioate］in the coastal environment of Cheju island, samples of sea organism, water and sediment were collected at the Sackdaldong and Daepodong near the Jungmoon golf course in July and Aug. 1997. The qualified detection limit of mono-crotophos and EPN by GC -FPD were 0.024 ng/g and 0.020 ng/g in the sea organism, respectively Neither Monocrotophos nor EPN was detected in seaweed cava (Ecklonia cava), Agar (Gelidium amansii), turban sell (Batillus cornutus) and sea urchin (Anthocidaris Crassispina). They were not detected in seawater and sediment, either. These results showed that the coastal area near the Jungmoon golf course in the Cheju island was not polluted by the organophosphorus pesticides such as Monocrotophos and EPN.

• Nuclear industry
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• v.15 no.2 s.144
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• pp.22-28
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• 1995

1994년도에 각 원전주변의 환경시료를 채취하여 방사능을 조사한 결과, 인공방사성핵종은 육상토양시료에서 Cs-137이 최대 31.9Bq/kg-dry, 해저토에서 최대 4.24Bq/kg-dry로 나타났으며, 일부 해양생물시료중 검출된 Co-58, Co-60, Mn-54 및 Ag-110m은 170Bq/kg 이내로 허용치 대비 0.08$\%$ 이내로 안전한 것으로 평가된다.

• Journal of the Korean earth science society
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• v.42 no.1
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• pp.1-10
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• 2021

Silicon (Si) is the second most abundant element in the crust and consists of three stable isotopes, 28Si (92.23%), 29Si (4.67%), and 30Si (3.10%). Si isotopes are widely studied worldwide as a proxy for the biogeochemical cycle of Si to reconstruct the paleoenvironment and paleoclimate. However, in Korea, there have been no studies on biogenic silica using Si isotopes. In this study, we carried out Si isotope measurements of giant diatoms, summarizing the previously reported alkali fusion methods and establishing the best Si separation method for biogenic silica. Samples were completely digested using alkali fusion at high temperatures, effectively separating Si using an AG® 50W-X8 cation exchange resin. To evaluate the precision and accuracy of our measurements, Si isotope standard material (NBS-28) and USGS reference materials (AGV-2, GSP-2, BHVO-2) were analyzed. The results are in excellent agreement with the reported values within the acceptable error. The Si isotope measurement method developed in this study is expected to help in understanding the paleoclimate and paleoenvironment by tracing the Si cycle.

• Environmental Analysis Health and Toxicology
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• v.18 no.2
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• pp.77-87
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• 2003

성게의 정자를 이용한 생물검정은 해양환경의 독성평가에 널리 사용되고 있다. 그러나, 국내산 성게류의 경우 산란기가 3~11월로 겨울에는 생물검정 실칩이 불가능한 단점이 있다. 이를 보완하기 위하여 겨울에 산란이 가능한 아무르불가사리(Arterias amurensis)를 생물검정용으로 개발하였다. 불가사리류의 생식과 발생과정은 성게류와 유사하므로 성게류를 이용한 실험 방법을 아무르불가사리에 적용할 수 있다. 그러나, 생물검정 실험 결과의 유효성은 실험조건에 의하여 달라질 수 있으므로, 본 연구에서는 실험 결과에 큰 영향을 미칠 수 있는 조건들 즉. 수정막의 발달 시간, 정자와 알의 비율, 정자의 활력이 유지되는 시간, 정자의 노출 시간, 그리고 시료의 염분 범위 등을 최적화 하고자 하였다. 수정막의 발달이 완전하게 이루어지는 최소 시간은 60분이었고, 시료에 주입할 정자와 알의 비율은 3,000이상일 때 수정률이 높게 유지되었다(70%이상). 정자의 활력은 해수에 희석한 후 3시간 이상이 경과하면 급격히 감소하였다. 정자의 노출시간은 20~60분의 범위에서 구리에 대한 $EC_{50}$이 크게 달라지지 않았다(변동계수: 17%).실험에 적절한 시료의 염분 범위는 26~38 psu이었다. 아무르불가사리 정자의 민감도는, 일반적으로 해양환경 평가에 흔히 사용되는 생물들의 민감도 범위의 중간 정도에 해당하였다. 따라서 아무르불가사리의 정자는 해양환경 평가를 위한 생물검정용으로 적합한 것으로 여겨진다.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.1
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• pp.83-88
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• 2008

Using micromill, discrete carbonate powders from the otolith of Argyrosomus argentatus were sampled along the growth band, and high-resolution stable isotope profiles were obtained. The ${\delta}^{18}O$ and ${\delta}^{13}C$ values are increasing gradually from the core to the margin. However, such increases do not seem to be attributed to the environmental property changes during the growth, but to the dominant effect of metabolic carbons during the early growth, and then, the isotopic composition seems to be equilibrium to the environmental condition. This paper allows the diverse applicability of high-resolution isotope research to the fish otoliths in the future.

• Proceedings of the Korean Quaternary Association Conference
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• 2004.11a
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• pp.75-76
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• 2004

일산 이산포 BH-4 발굴단면의 퇴적물 시료로부터 화분 포자 및 담수성과 해양성조류화석이 다양하고 풍부하게 산출되었다. 산출된 식물기원 미화석 중, 목본화분 $25{\sim}60%$, 초본화분 $15{\sim}70%$ 그리고 포자 $4{\sim}30%$ 각각 구성 되었다. 또한 담수성 조류(freshwater algae)는 전체 단면구간에서 산출된 반면, 와편모류(dinoflagellate), 아크리타치(acritarch)와 같은 해양성조류(marine algae)는 하부단면구간(절대고도: 3.31-2.74 m)에서만 산출되었다. 산출된 화분 포자 및 통계처리기법(CONISS)을 이용하여 2개의 지역화분대 (local pollen a assemblage zone) 및 4개의 아화분대(local pollen assemblage subzone)가 설정되었다. 제I화분대(시료 $Al-2{\sim}A6-1$, 고도 $2.70{\sim}3.31\;m$)는 다시 제Ia아화분대(시료 $Al-2{\sim}A3-1$, 고도 $2.70{\sim}3.00\;m$)와 제Ib아화분대(시료 $A4-2{\sim}A6-1$, 고도 $3.01{\sim}3.31\;m$)로 세분되었으며; 제II화분대(시료 $A7-2{\sim}A10-1$, 고도 $3.32{\sim}3.72\;m$) 역시 두개의 아화분대인 제IIa(시료 $A7-2{\sim}A8-1$, 고도 $3.32{\sim}3.51\;m$)와 제lIb(시료 $A9-2{\sim}A10-1$, 고도 $3.53{\sim}3.72\;m$)로 이루어졌다. 화분군집조성에 의하면, 미화석들이 퇴적되었던 시기동안에 2개의 산림(forest)형태가 발달했던 나타났다. 제I화분대(고도, $3.31{\sim}2.74\;m$)의 시기동안에는 침엽수와 낙엽활엽수로 이루어진 혼합림으로, 주요 식생은 2엽소나 무(Diploxylon), 가문비((Picea), 낙엽송(Larix)의 침엽수와 참나무(Quercus), 자작나무(Betula), 개암나무(Corylus), 중국굴피 나무속(Pterocarya)의 낙엽활엽수 등이 산악-구릉지에서 주로 서식하였던 것으로 나타났다. 또한 습생나무인 오리나무(Alnus), 버드나무(Salix)등과 해안가 등지에서 잘 서식하는 양치식물과 사초과(Cyperaceae), 명아주과(Chenopodiaceae) 같은 풀들이 하구언(estuary)의 해안가에 주로 서식하였던 것으로 나타났다. 이 시기동안에 해양성조류인 와편모류와 담수성 조류가 동시에 다양하게 산출되는 것은 하구언 퇴적환경이었음을 잘 지시해 준다. 제II화분대(고도, 43.72{\sim}3.36\;m$)가 시작되면서 혼합림은 침엽수림으로 바뀌었으며, 낙엽활엽수들의 서식지가 2엽소나무로 대치되면서 침엽수가 주요 수종을 이루었던 것으로 나타났다. 아마도 이런 산림형태는 자연 기후환경변화 뿐만 아니라 인간간섭에 의해 나타난 결과로 생각된다. 이 시기에 경작식물인 벼과(Gramineae), 옥수수(Zea), 메밀(Fagopyrum)과 논밭의 뚝 등지 에서 서식하는 돼지풀(Ambrosia) 등이 처음으로 산출되는 것으로 보아 인간간섭이 행해졌음을 잘 뒷 받침해준다. 또한 담수성 조류는 변함없이 산출되는 반면, 해양성조류인 와편모류가 산출되지 않는 것으로 보아 해안환경에서 다소 멀 어진 저지대의 습윤한 충적대지환경으로 변하였던 것으로 판단된다.

• Journal of the Korean Geotechnical Society
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• v.37 no.8
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• pp.5-13
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• 2021

Liquefaction behaviors of two natural silty samples containing fines including clay of 50% or more (Sample No.1 was silt 44.1% and clay 8.8%, sample No.2 was silt 57.2% and clay 12.4%) were examined by a cyclic triaxial test. According to the results on samples containing 50% or more of fine particles, an increase in the fine content decreases the liquefaction resistance of the sample. In other words, when the fine content increases, the liquefaction state of sample is reached with a small number of cyclic loads. In the relationship between the excess pore water pressure ratio and the number of cycles, the slope of the excess pore water pressure ratio increases more steeply as the fine content increases. As a result of analyzing the liquefaction behavior of the two silts with the content of clay contained in the fines, liquefaction occurred more easily in the No.2 silt with high clay content. This result shows that the clay contained in the fines affects the liquefaction behavior of the silt.

• Korean Journal of Environmental Biology
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• v.37 no.1
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• pp.31-41
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• 2019

The toxic dinoflagellate Gymnodinium catenatum isolated from the southern coast of Korea was described under light and scanning electron microscopy, and its large subunit (LSU) rDNA was sequenced. In addition, the effects of temperature and salinity on its growth were investigated. The cells of G. catenatum, as viewed under the electronic microscope, were green-brown color, $38.1-77.4{\mu}m$ in length and $26.1-40.8{\mu}m$ in width. The epicone was conical, while the hypocone was trapezoidal. The nucleus was located at the central part of the cell. The apical groove was horseshoe-shaped and small pores were irregularly distributed on the cell surface. Molecular phylogeny based on LSU rDNA gene sequences showed that the Korean G. catenatum and previously reported species formed a monophyletic clade within Gymnodinium sensu stricto clade. The maximum growth rate of $0.37day^{-1}$, was obtained at $25^{\circ}C$ and 35 psu, and the maximum cell density of $1,073cells\;mL^{-1}$, was observed at $20^{\circ}C$ and 25 psu. However, G. catenatum did not grow at temperature < $15^{\circ}C$ and < $30^{\circ}C$. These results suggest that environmental conditions of summer and autumn in the southern coast of Korea may be favorable for the growth of G. catenatum.

• Korean Journal of Environmental Biology
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• v.40 no.4
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• pp.387-397
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• 2022

The germination characteristics of the resting cysts of Pheopolykrikos hartmannii collected from the southern coastal sediments of Korea were studied at different temperature conditions, and the morphology and phylogeny of the germlings were examined. The resting cysts of Ph. hartmannii were round and characterized by a red accumulation body and many arrow-like spines and could germinate at temperature of 10 to 30℃. High germination rates (>90%) were observed at 15 and 20℃, indicating that the resting cysts could act as seed populations for the bloom initiation of Ph. hartmannii in Korean coastal waters in early summer or early fall. The morphology of the germlings was generally consistent with the previous description, and an apical groove characterized by a fully enclosed loop was observed. Phylogenetic analysis based on large SubUnit (LSU) rRNA gene sequences revealed that the germlings shared an identical sequence with the Korean and American isolates of Ph. hartmannii and was a sister clade of Polykrikos species.