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Silicon Isotope Measurement of Giant Diatoms Using MC-ICP-MS

다검출기 유도결합 플라즈마 질량분석기를 이용한 대형 규조류 규소 동위원소 분석법

  • Choi, Ah Yeong (Department of Geological Sciences, BK21 School of Earth and Environmental Systems, Pusan National University) ;
  • Ryu, Jong-Sik (Department of Earth and Environmental Sciences, Pukyong National University) ;
  • Hyeong, Kiseong (Global Ocean Research Center, Korea Institute of Ocean Science and Technology) ;
  • Kim, Mun Gi (Global Ocean Research Center, Korea Institute of Ocean Science and Technology) ;
  • Ra, Kongtae (Marine Environmental Research Center, Korea Institute of Ocean Science and Technology) ;
  • Jeong, Hyeryeong (Marine Environmental Research Center, Korea Institute of Ocean Science and Technology) ;
  • Lim, Hyoun Soo (Department of Geological Sciences, BK21 School of Earth and Environmental Systems, Pusan National University)
  • 최아영 (부산대학교 지질환경과학과) ;
  • 류종식 (부경대학교 지구환경과학과) ;
  • 형기성 (한국해양과학기술원 대양자원연구센터) ;
  • 김문기 (한국해양과학기술원 대양자원연구센터) ;
  • 나공태 (한국해양과학기술원 해양환경연구센터) ;
  • 정혜령 (한국해양과학기술원 해양환경연구센터) ;
  • 임현수 (부산대학교 지질환경과학과)
  • Received : 2021.02.09
  • Accepted : 2021.02.17
  • Published : 2021.02.28
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Abstract

Silicon (Si) is the second most abundant element in the crust and consists of three stable isotopes, 28Si (92.23%), 29Si (4.67%), and 30Si (3.10%). Si isotopes are widely studied worldwide as a proxy for the biogeochemical cycle of Si to reconstruct the paleoenvironment and paleoclimate. However, in Korea, there have been no studies on biogenic silica using Si isotopes. In this study, we carried out Si isotope measurements of giant diatoms, summarizing the previously reported alkali fusion methods and establishing the best Si separation method for biogenic silica. Samples were completely digested using alkali fusion at high temperatures, effectively separating Si using an AG® 50W-X8 cation exchange resin. To evaluate the precision and accuracy of our measurements, Si isotope standard material (NBS-28) and USGS reference materials (AGV-2, GSP-2, BHVO-2) were analyzed. The results are in excellent agreement with the reported values within the acceptable error. The Si isotope measurement method developed in this study is expected to help in understanding the paleoclimate and paleoenvironment by tracing the Si cycle.

규소(Si)는 지각의 구성 원소 중 두 번째로 흔히 존재하는 원소로, 3개의 안정동위원소, 28Si (92.23%), 29Si(4.67%), 30Si (3.10%)를 가진다. 규소 동위원소는 규소의 생지화학적 순환에 대한 지시자로 고환경 및 고기후 복원을 위해 전 세계에서 널리 연구되고 있다. 그러나 국내에서는 아직까지 생물 기원 규소에 대한 규소 동위원소 연구가 전무한 실정이다. 본 연구에서는 대형 규조류 시료에 대한 규소 동위원소 분석을 위해 기존 보고된 알칼리 용융법을 정리하고 생물 기원 규소 분석에 가장 적합한 규소 분리법을 구축하고자 하였다. 해당 시료를 고온 알칼리 용융을 통해 완전 용해시킨 후 시료 내 규소를 AG® 50W-X8 양이온 교환수지를 이용하여 효과적으로 분리하였다. 분리된 시료에 대한 신뢰성 검증을 위하여 Si 동위원소 표준물질(NBS-28) 및 USGS 암석 표준시료(AGV-2, GSP-2, BHVO-2)에 대한 분석을 함께 실시하였으며, 분석된 시료 모두 기존 연구결과와 오차범위 내에서 일치하는 값을 나타내었다. 본 연구에서 개발한 규소 동위원소 분석법은 향후 국내의 지구과학 및 관련 연구 발전에 많은 도움을 줄 것으로 기대된다.

Keywords

Acknowledgement

이 논문은 부산대학교 국립대학육성사업(2019-2020)의 지원을 받아 연구되었습니다. 퇴적물 시료를 제공해 주신 한국해양과학기술원 대양자원연구센터(PE99924)에도 감사드립니다.

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