• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.9
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• pp.871-877
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• 2015

Deep-sea equipment such as underwater robots and unmanned submersible vehicles, include various machine components and sensors, and it is important that their reliabilities be tested before use in the fields. This is necessary because they are affected by complex extreme-environment conditions, such as high pressures, extreme temperatures, and tidal forces that are present in the deep sea. We require test equipment that can conduct empirical tests in conditions that mimic these complex oceanic environments. In this study, we propose specifications that should be met, and a design plan for the primary components, which should limit their use to a maximum water pressure of 2.0 MPa, water temperature of $5{\sim}60^{\circ}C$, and a maximum flow velocity of 2 m/s. in work-in type underwater combined environment test equipment and. We present test system development procedures to verify the reliability of products and systems used in deep-sea environments.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.1
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• pp.43-51
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• 2005

This paper presents a waste load allocation study for the Incheon coastal environment, where a computer model, called AQUASEA, was applied. A finite element mesh was constructed and refined to cover the complicated geometry of Incheon coastal sea. The tidal height at 13 places of Incheon coastal boundary and flow of the Han River were given as an input condition to the tidal simulation. All pollution sources that discharge into Incheon coast were given as input data to the water quality simulation. The modeled parameters include tidal flow and COD(Chemical Oxygen Demand). The model was calibrated and verified with the field measurements. The model results showed reasonable agreements with field measurements in both tidal flow and water quality. Systems analysis showed that the pollution load from the Han River caused recognizable impacts on the water quality of Incheon coast from Yeomhwa waterway to northern area of Younghungdo. The loads from Incheon City affected water quality from the area below Youngjongdo to the area above Jawalldo. The discharge from the Sihwa Lake caused discernible impacts on the coastal zone from the dike outlet to the Incheon harbor, and pollution loads from Kyungkido affected the sea near the Oido. An effective water quality management plan was developed from the waste load allocation analysis of the validated model, that the maximum waste loads can be discharged without violating the water quality standard given in the Incheon coastal environment.

• Korean Journal of Microbiology
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• v.47 no.1
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• pp.22-29
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• 2011

Rhodopsin is a membrane protein with seven transmembrane region which contains a retinal as its chromophore. Although there have been recently reports on various photo-biochemical features of rhodopsins by a wide range of purifying and measurement methods, there was no actual comparison related to the difference of biochemical characteristics according to their physical environment of rhodopsins. First, proteorhodopsin (PR) was found in marine proteobacteria whose function is known for pumping proton using light energy. Second one is Anabaena sensory rhodopsin (Nostoc sp.) PCC7120 (ASR) which belongs to eubacteria acts as sensory regulator since it is co-expressed with transducer 14 kDa in an operon. In this study, we applied two types of rhodopsins (PR and ASR) to various environmental conditions such as in Escherichia coli membranes, membrane in acrylamide gel, in DDM (n-dodecyl-${\beta}$-D-maltopyranoside), OG (octyl-${\beta}$-D-glucopyranoside), and reconstituted with DOPC (1,2-didecanoyl-sn-glycero-3-phosphocholine). According to the light-induced difference spectroscopy, rhodopsins in 0.02% DDM clearly showed photointermediates like M, and O states which respond to the different wavelengths, respectively and showed the best signal/noise ratio. The laser-induced difference spectra showed the fast formation and decay rate of photointermediates in the DDM solubilized samples than gel encapsulated rhodopsin. Each of rhodopsins seemed to be adapted to its surrounding environment.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2009.04a
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• pp.108-108
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• 2009

The main object of this research is to minimize the shock effects which frequently result in fatal damage in wind met mast on impact of barge. The collision between wind met mast and barge is generally a complex problem and it is often not practical to perform rigorous finite element analyses to include all effects and sequences during the collision. LS-dyna generally purpose explicit finite element code, which is a product of ANSYS software, is used to model and analyze the non-linear response of the met mast due to barge collision. A significant part of the collision energy is dissipated as strain energy and except for global deformation modes, the contribution from elastic straining can normally be neglected. On applying impact force of a barge to wind met mast, the maximum acceleration, internal energy and plastic strain were calculated for each load cases using the finite element method and then compare it, varying to the velocity of barge, with one varying to the thickness of rubber fender conditions. Hence, we restrict the present research mainly to the wind met mast and also parametric study has been carried out with various velocities of barge, thickness of wind met mast, thickness and Mooney-Rivlin coefficient of rubber fender with experimental data. The equation of motion of the wind met mast is derived under the assumption that it was ignored vertical movement effect of barge on sea water. Such an analyzing method which was developed so far, make it possible to determine the proper size and material properties of rubber fender and the optimal moving conditions of barge, and finally, application method can be suggested in designing process of rubber fender considering barge impact.

• Journal of Navigation and Port Research
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• v.38 no.3
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• pp.239-246
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• 2014

A study on the elution characteristics of biostimulating agents (sulfate and nitrate) from biostimulants which are used for in-situ bioremediation for the coastal sediment contaminated with organic matter was performed. The biostimulating agents were mixed with the coastal sediment, and then massed the mixture into ball. Two kinds of ball type biostimulant were prepared by coating the ball surface with two different polymers, cellulose acetate and polysulfone. A granular type biostimulant (GTB) was also prepared by impregnating a granular activated carbon in the biostimulating agent solution. The image of scanning electron microscopy for the biostimulant coated with cellulose acetate (CAB) showed that the inner side of the coating layer consisted of irregular and bigger size of pores, and the surface layer had tight structure like beehive. For the biostimulant coated with polyfulfone (PSB), the whole coating layer had a fine structure without pore. The elution rate of the biostimulating agents for the CAB was higher than that for the PSB, and the elution rate for the GTB was considerably higher than that for the PSB in distilled water as well as in sea water. The elution rate of the biostimulating agents in turbulent water flow was about 3 times higher than that in standing water, and the elution rate of nitrate was higher than that of sulfate from the stimulating agents.

• International Journal of Highway Engineering
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• v.5 no.3 s.17
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• pp.11-20
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• 2003

Concrete structures such as bridges, pavement, and offshore structures are normally subjected to repeated load. Because highway and airfield pavements are to resist tension in bending, fatigue failure behavior is very important the fatigue life of materials. Therefore, in this paper was carried according to the fatigue test method and experiment variables for pavement concrete. The fatigue tests were applied split tension($150{\times}75$ in size) and flexural($150mm{\times}150mm{\times}550mm$ in size) beam fatigue test method. Major experimental variable in the fatigue tests in order to consideration of fatigue life were conducted loading frequency of 1, 5, 10, 20Hz and loading shape of block, sine, triangle and moisture condition of dry and wet condition and curing age of 28day and 56day. The test results show that the effect of loading frequency increasing the frequency increased fatigue life, decreased significant at frequencies below 200 cycles. The effect of loading wave form on fatigue life show that a block decreased, triangular increased in comparison with sine. The effect of moisture condition decreased in wet condition in comparison with dry condition. The effect of curing age increased in 564ays in comparison with 28day.

• Journal of Life Science
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• v.21 no.11
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• pp.1599-1606
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• 2011

To evaluate the effects of microorganism agents on oil biodegradation, treatability and microcosm studies were conducted. Petroleum oil degrading bacteria were isolated from enriched cultures of oil-contaminated sediment samples using a mineral salts medium (MSM) containing 0.5% Arabian heavy crude oil as the sole carbon source. After a 5 day-incubation period using MSM, mixed microorganisms of three species (strains BS1, BS2 and BS4) degraded 48.4% of aliphatic hydrocarbons and 30.5% of aromatic hydrocarbons. Treatability and microcosm tests were performed in the three different treatment conditions (AO: Arabian heavy crude oil, AO+IN: Arabian heavy crude oil+inorganic nutrient, AO+IN+MM: Arabian heavy crude oil+inorganic nutrient+mixed microorganism agents). Among these, significantly enhanced biodegradation of aliphatic hydrocarbons were observed in AO+IN and AO+IN+MM conditions, without showing any different biodegradation rates in either condition. However, the degradation rates of aromatic hydrocarbons in an AO+IN+MM condition were increased by 50% in the treatability test and by 13% in the microcosm test compared to those in an AO+IN condition. Taken together, it can be concluded that mixed microorganism agents enhance the biodegradation of aliphatic and aromatic hydrocarbons in laboratory, a treatability test, and a microcosm test. This agent could especially be a useful tool in the application of bioremediation for removal of aromatic hydrocarbons.

• KSBB Journal
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• v.17 no.6
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• pp.571-576
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• 2002

Recombinant E. coli DH5 ${\alpha}$/pSB311 was made by cloning the genes encoding bacterial luciferase and aldehyde substrate proteins from Photohabdus luminescense, to complement defects of Lumistox, which is normally used in bioassays to monitor toxic substances in water environmental systems. The conditions for stable light production by the recombinant strains were investigated with respect to cell growth stage, cell number, and buffer conditions. The optimum growth stage was a middle-exponential stage with an OD$_{660nm}$ value of 0.6-0.7. ADout 10$^{6}$-10$^{7}$ cells per test tube was optimum for stable light emission. The effect of buffer was not significant if an optimum viable cell number was maintained. The bioluminescence of the recombinant E. coli harboring the lux operon of Photohabdus luminescense was not affected by temperature, while the bioluminescence of Lumistox was temperature sensitive. The recombinant E. coli was more sensitive to heavy metals (Cd, Cu, Hg, Zn) than Lumistox, because it does not require high concentrations of NaCl in the buffer.

• Journal of Life Science
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• v.24 no.8
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• pp.880-886
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• 2014

In this study, we isolate and identify bacteria from seawater collected from Jeju coast, to evaluate the antimicrobial activity against the fish pathogenic bacteria. 14 bacterial strains were isolated and identified using physiological, biochemical and molecular tools. Antibacterial activity of all the 14 isolates were screened against four major fish pathogens namely, two Gram-positive: Streptococcus iniae, Streptococcus parauberis and two Gram-negative: Vibrio anguillarum, Edwardsiella tarda. Results revealed that among the 14 isolates, MK-11 was found to have antibacterial activity against S. iniae, S. parauberis, V. anguillarum Particularly, S. iniae was susceptibility with the MIC value of $250{\mu}g/ml$. The biochemical and physio-chemical results reveal that MK-11 had the sugar-alcohol disassemble ability of the D-sorbitol and D-mannitol. Also the utilization of the yeast extract, sorbitol and di-potassium phosphate were noted to be high. The optimum culture condition such as pH and temperature was recorded as pH 6.0, $25^{\circ}C$ and along with 1% NaCl which differs from the previous reports particularly in nutrient resolutions. As results of the analysis of 16S rDNA sequences, MK-11 show the high similarity with Paenibacillus polymyxa, P. jamilae, P. brasilensis 99.78%, 99.43%, 99.39%, repectively. Hence, in the present study, the isolated Paemibacillus sp. MK-11 from Jeju seawater possesses the antibacterial activity against fish pathogens and it could be used as a new antibiotic agents against the gram positive fish pathogens.

• Journal of IKEEE
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• v.24 no.1
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• pp.347-353
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• 2020

In this paper, the durability of an electromagnetic metamaterial absorber is verified in a temperature varying condition mimicking a maritime environment for the purpose of applying it to reduce the radar cross section of an integrated mast of the next-generation destroyer. To validate the durability, the reflectance of the electromagnetic metamaterial absorber was measured after storing it in a chamber that can control the temperature according to Procedure I of Method 501.7 included in MIL-STD-810H. Before and after the environmental test, both of the measured reflectances were retained less than -10 dB over the X band, that can guarantee the stealth functionality.