• Title/Summary/Keyword: 항미생물

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Antimicrobial Substances in Leek (Allium tuberosum) (부추의 항미생물 활성물질)

  • Kim, Seon-Jae;Park, Keun-Hyung
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.604-608
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    • 1996
  • The antimicrobial activty of leek (Allium tuberosum) was investigated against 17 strains of microorganisms. Methanol extracts of leek showed the growth inhibition effects on the wide range of microorganisms including gram positive bacteria, gram negative bacteria and yeasts. The extracts were analysed by using solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 column chromatography, TLC, silica gel partition chromatography and HPLC techniques. Six components whose molecular weights range from 200 to 400 were confirmed to have the antimicrobial activity.

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Purification and Identification of Antimicrobial Substances in Phenolic Fraction of Fig Leaves (무화과잎 페놀성 분획중의 항미생물 활성물질의 정제 및 동정)

  • Kang, Seong-Kuk;Chung, Dong-Ok;Chung, Hee-Jong
    • Applied Biological Chemistry
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    • v.38 no.4
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    • pp.293-296
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    • 1995
  • Fig leaves were extracted with methanol and then fractionated with ethyl acetate and various buffers to get active fractions and determined the antimicrobial activities. The acidic and phenolic fractions fractionated from the methanol extract of fig leaves showed the strong antimicrobial activities, but the basic and neutral fractions did not show any activities. The degree of antimicrobial activities of phenolic fraction against tested bacteria was higher than those of acidic fraction, but these against yeasts and mold were almost equivalent to those of acidic fraction. Especially, phenolic fraction was mostly affected on Staphylococcus aureus and Pseudomonas aeruginosa. Four antimicrobial substances purified from the phenolic fraction which showed the strongest antimicrobial activities among the fractions from fig leaves, were identified as psoralen($C_{11}H_{6}O_{3}$, MW. 186), bergapten($C_{12}H_{8}O_{4}$, MW. 216), ${\beta}$-sitosterol($C_{29}H_{50}O$, MW. 414) and umbelliferone ($C_{9}H_{6}O_{3}$, MW. 162).

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Isolation and Identification of Pratensein with Antimicrobial Activity from the Peanut Shells (땅콩껍질에서 항미생물 활성을 지닌 pratensein의 분리 및 동정)

  • Wee, Ji-Hyang;Moon, Jae-Hak;Park, Keun-Hyung
    • Korean Journal of Food Science and Technology
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    • v.36 no.4
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    • pp.643-647
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    • 2004
  • Natural antimicrobial substance from peanut (Arachis hypogaea) shells was isolated and structurally elucidated. Peanut shells were extracted with methanol (MeOH) and concentrated in vacuo, MeOH extract was solvent-fractionated with ethyl acetate (EtOAc) and various buffer to obtain EtOAc acidic, neutral, and phenolic fractions. EtOAc neutral fraction, which showed antimicrobial activity, was purified through silica gel adsorption column, Sephadex LH-20 column, ODS column, and high performance liquid chromatographies, and its active substance was isolated and identified as pratensein (3',5,7-trihydroxy-4'-methoxyisoflavone) by spectroscopic methods of proton-nuclear magnetic resonance, mass spectrometry, and nuclear overhauser enhancement spectroscopy.

The anti-plaque effect of mouthwash containing Hinoki essential oil (편백 에션셜 오일 함유 구강양치액(가글)의 치면세균막 형성 억제 효과)

  • An, So-Youn;Seong, Jeong min;Jeong, Mi Ae
    • Proceedings of the Korea Contents Association Conference
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    • 2017.05a
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    • pp.409-410
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    • 2017
  • 본 연구는 편백 에션셜 오일을 이용하여 치아우식증과 치주질환을 예방하기 위한 효과적인 구강양치액을 연구개발하고자 수행되었다. Streptococcus mutans에 대한 항미생물 효과 및 치면세균막 형성에 미치는 영향을 실험실연구(in vitro)와 전임상연구(preclinical observations)를 통해 조사하였다. 편백에센셜오일은 실험실 연구결과 S. mutans 성장 억제효과와 항미생물 효과를 보였고, 전임상시험 결과 자원자들의 치면세균막 지수가 감소하였다. 편백 에센셜 오일은 뛰어난 항미생물 효과와 치면세균막 억제를 보이는 것으로 나타났다.

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신선한 돈육 등심과 삼겹에 첨가한 비타민 C 전구체, 마늘 그리고 양파 분말의 항산화 및 항미생물 효과

  • Park, Seong-Yong;Jin, Gu-Bok
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2006.05a
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    • pp.161-165
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    • 2006
  • 삼겹에 있어서 SA, 양파 그리고 마늘의 첨가로 FFA 값은 저장 28일에 대조구와 비교하여 각각 14, 31 그리고 53% 감소하였으나 등심에서는 효과가 없었다. 삼겹에서 과산화물의 생성률은 SA처리구를 제외하고 천연소제 첨가에 의해 감소되었다. 그러나 등심에서는 마늘을 제외하고는 천연소제 첨가에 의한 효과는 없었다. 삼겹과 등심 모두에서 휘발성 산화 생성물들은 천연소제에 의한 주목할 만한 효과는 없었다. 삼겹에서 마늘과 양파는 저장성을 연장시킴으로써 항미생물 효과를 보였다. 본 연구에 사용된 천연 물질들은 항산화 및 항미생물 효과를 보였다. 특히 마늘은 지방을 포함하는 식품에서 안정화를 위한 천연 자원으로써 고려되어져야 할 것이다.

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Isolation and Characterization of 4-Hydroxy-3-methoxycinnamic Acid and 3,4-Dihydroxycinnamic Acid with Antimicrobial Activity from Root of Pulsatilla koreana (백두옹에서 항미생물 활성을 갖는 4-Hydroxy-3-methoxycinnamic Acid와 3,4-Dihydroxycinnamic Acid의 분리 및 동정)

  • Lee, Hyang-Hee;Ma, Seung-Jin;Moon, Jae-Hak;Park, Keun-Hyung
    • Applied Biological Chemistry
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    • v.41 no.2
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    • pp.191-196
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    • 1998
  • The MeOH extract from root of Pulsatilla koreana was showed antimicrobial activities against bacteria and yeast. The antimicrobial active substances of MeOH extract were successfully purified with solvent fractionation, silica gel adsorption column chromatography and Sephadex LH-20 column chromatography. The purified two active substances were isolated by HPLC and identified as 4-hydroxy-3-methoxycinnamic acid and 3,4-dihydroxycinnamic acid by MS, $^{1}H-NMR$ and $^{13}C-NMR$.

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Antibacterial Finish of Cotton Fabric with Silver Nanoparticles (은 콜로이드를 이용한 면직물의 항균가공)

  • 이은지;정성훈;이범수
    • Proceedings of the Korean Fiber Society Conference
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    • 2001.10a
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    • pp.390-391
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    • 2001
  • 은의 항균작용은 예로부터 알려져 왔는데, 특히 천연섬유에 기생하는 세균(bacteria)과 곰팡이(fungi) 등의 미생물을 제거하여 질병유발, 악취, 제품의 오염 방지에 효과적이다. 은은 다른 가공제와 달리 소량으로 항미생물 효과를 얻을 수 있으므로 나노 사이즈의 은 입자를 농도별로 면직물에 처리하고 세탁에 대한 내구성을 알아보고자 하였다. 이에 따라, 소량으로도 항미생물 효과를 나타내는 최적의 공정을 구하고 은입자가 균일하게 처리되는 조건에서 가공제의 내세탁성을 조사하였다. (중략)

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Comparison of biological activities of MeOH extracts in different cultivars and organs of Catharanthus roseus (L.) G.Don (일일초 품종 및 부위별 메탄올 추출물 활성 비교)

  • Lee, Hui-Kyoung;Kim, Ju-Sung;Kim, Myong-Jo;Heo, Kwon;Lee, Hyeon-Yong;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.3
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    • pp.212-216
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    • 2002
  • Catharanthus roseus was extracted with methanol and its Vinblastine contents and antioxidant and antimicrobial activities in each methanol extracts were determined. Vinblastine contents of leaves were much higher than those in other plant parts. The highest DPPH(1,1-diphenyl-2 -picrylhydrazy) radical scavenging activities in plnat parts showed in leaves of Stardust Orchid and Cooler Rose and the value was $RC_{50}=17\;and\;18\;{\mu}g/ml$ respectively. Leaves of Cooler Rose and Coller Strawberry showed strong antimicrobial activities. Particularly, Cooler Rose showed strong antimicrobial activites except to Penicillium oxalicum.

Solvent Fractionation of Fig Leaves and its Antimicrobial Activity (무화과 잎의 용매분획 및 항미생물 활성)

  • Kang, Seong-Kuk;Chung, Hee-Jong
    • Applied Biological Chemistry
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    • v.38 no.4
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    • pp.289-292
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    • 1995
  • Fig leaves were extracted with methanol and then fractionated with ethyl acetate and various buffers to get active fractions, and their antimicrobial activities in each fraction were determined. Acidic fraction and phenolic fraction of fig leaves showed strong antimicrobial activities, but the basic fraction and neutral fraction did not show any activities. The degree of antimicrobial activities in phenolic fraction against tested bacteria were higher than those in acidic fraction, but these against yeasts and mold were almost equivalent to those in acidic fraction. Especially, phenolic fraction showed the strongest activities against Staphylococcuns aureus and Pseudomonas aeruginosa. Although there were some differences among microorganisms, minimum inhibition concentrations(MIC) in acidic and phenolic fractions against tested microorganisms were 0.05 to 0.1 g eq./ml and 0.01 to 0.25 g eq./ml, on the basis of fresh sample weight, respectively. Minimum lethal concentrations(MLC) in acidic and phenolic fractions were 0.25 to 2.00 g eq./ml and 0.05 to 1.50 g eq./ml, respectively. These supposed the antimicrobial activities in phenolic fraction were generally higher than those in acidic fraction and also phenolic fraction had lower MIC and MLC values than acidic fraction.

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Screening of the Natural Plant Extracts for the Antimicrobial Activity on Dental Pathogens (구강 질환 유발 미생물에 대한 항생작용을 갖는 천연물 추출물 검색)

  • Baek, Dong-Heon
    • Korean Journal of Microbiology
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    • v.43 no.3
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    • pp.227-231
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    • 2007
  • Many of antimicrobial components used in the mouthwash have disadvantages such as coloring of periodontal tissue and homing sensation. To find the more biocompatible antimicrobial agent, we have screened the 2,125 kinds of natural plant extracts obtained from by the Plant Extract Bank (Daejeon, Korea). The antimicrobial activity was determined by spectrophotometric growth inhibition assay for the major dental pathogens. For the Porphyromonas gingivalis, 19 plant extracts had an antimicrobial activity, 17 plant extracts for the Prevotella intermedia, 45 plant extracts for the Haemophilus actinomycetemcomitans and 85 plant extracts for the Streptococcus mutans. Among these effective extracts, 8 kind of natural plant extracts had an antimicrobial activity for more than 3 species of dental pathogens. In our experiment, Cudrania tricuspidata, Morus bombycis and Mallotus japonicas have antibacterial effects on the all kinds of major dental pathogens. Therefore these plant extracts have a possibility to be a candidate for major antibacterial components in dental products.