• Title/Summary/Keyword: 항균 특성

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The Cultural Characteristics and Antibacterial Activities of Cordyceps militaris and Paecilomyces tenuipes (번데기동충하초와 눈꽃동충하초의 배양특성 및 항균활성)

  • Lee, Ki-Man;Hong, In-Pyo;Nam, Sung-Hee;Sung, Gyoo-Byung;Bae, Yoon-Hwan
    • Korean journal of applied entomology
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    • v.47 no.4
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    • pp.479-486
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    • 2008
  • The cultural characteristics and antibacterial activities of Cordyceps militaris and Paecilomyces tenuipes were compared. The mycelial growth was the highest on MCM (Mushroom Complete Medium) for C. militaris and on YMA (Yeast Malt Agar) for P. tenuipes. But the mycelial density on MMM (Mushroom Minimal Medium) was lower than other on media. The optimum mycelial growth was observed at $25^{\circ}C$. C. militaris was low mycelial growth when it was transferred over 5 times generation. The carbon source for the optimum mycelial growth was fructose of monosaccharide, maltose of disaccharide and dextrin of polysaccharide. The calcium nitrate of organonitrogen was found the best mycelial growth on C. militaris, while the sodium nitrate observed to be well for mycelial growth on P. tenuipes. The ammonium tartrate was observed to be the best among the inorganonitrogen used for mycelial growth. Antibacterial activities were found out just C. militaris against Bacillus cereus of Gram (+).

A Study on the Surface and Antibacterial Properties for M(Cd, Cu)-Activated Carbon (M(Cd, Cu)-활성탄의 표면 특성과 항균성에 관한 연구)

  • Oh, Won-Chun;Kim, Jong-Gyu;Kim, Myung-Kun
    • Analytical Science and Technology
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    • v.12 no.2
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    • pp.105-110
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    • 1999
  • The studies on the adsorption properties and the antibacterial effects of the Cd and Cu-treated activated carbon were carried out. From the adsorption studies on the series of these metal-treated activated carbons, typical Type-I isotherm was observed. The surface areas of the treated carbon obtained from BET equation were in the range of $1101-1418m^2/g$ for Cd-AC and of $1084-1361m^2/g$ for Cu-AC. Using ${\alpha}_s$-plot, the micropore volumes and pore size distribution were obtained. From the SEM study, it is also observed that many of micropores in activated carbon are blocked by window blocking effect of metals after the impregnation. Finally, antibacterial effects of M-activated carbon against Escherichia coli was discussed. From the study, the area of antibacterial activity becomes larger with the increase of the amount of metal treated.

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Characterization of the Bacteriocin Produced by a Leuconostoc mesenteroides Strain Inhibiting the Growth of Lactobacillus sakei (Lactobacillus sakei 생육저해활성 보유 Leuconostoc mesenteroides가 생산하는 Bacteriocin의 특성)

  • Lee, Kwang-Hee;Lee, Jong-Hoon
    • Microbiology and Biotechnology Letters
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    • v.39 no.4
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    • pp.390-396
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    • 2011
  • Lactobacillus sakei is known to be the most populous lactic acid bacteria in over-ripened kimchi. Twenty three strains of Leuconostoc species inhibiting the growth of Lb. sakei were isolated from kimchi and amongst these the Leuconostoc mesenteroides strain CK0122 exhibited the highest antagonistic activity against Lb. sakei. The culture supernatant of the strain CK0122 was fractionated by a molecular weight cutter and lyophilized. The fraction with a molecular weight of less than 3,000 Da showed antagonistic activity against Lb. sakei. The antibacterial activity of the active fraction was sensitive to proteinase K treatment, confirming its proteinaceous nature (bacteriocin). The crude bacteriocin was active in the pH range of 4 to 7 and extremely stable after 15 min of heat treatment at $121^{\circ}C$. The crude bacteriocin inhibited the growth of Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Alcaligenes xylosoxydans, Flavobacterium sp., and Salmonella typhimurium.

Screening and Characterization of Probiotic Strains for Prevention of Bacterial Fish Diseases (어류의 세균성 질병 예방을 위한 Probiotic균주의 선발 및 특성)

  • 허문수;양병규;전유진
    • Microbiology and Biotechnology Letters
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    • v.31 no.2
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    • pp.129-134
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    • 2003
  • The purpose of the present study was to screen the effective of lactic acid bacteria (LAB) as probiotics, which are able to protect aquacultural fish pathogenic bacteria, and investigate their characterization. Twenty strains of lactic acid bacteria were isolated from fish intestine, fermented fish foods and kimchis. These bacteria were screened for antagonistic activity against fish pathogenic bacteria. Seven tested LAB strains were able to inhibit the fish pathogenic bacteria, including Vibrio anguillarum, Edwardsiella tarda, and Streptococcus sp.. Of the probiotic candidates, BK19 strain isolated from fermented pollack viscera indicated the largest inhibition activity. Moreover, this strain showed a resistance over low pH and antibiotic agents. Therefore this probiotic candidate BK19 was finally selected and identified as a probiotic strain. This particular probiotic bacteria was identified as Lactobacillus sakei BK19 by biochemical characteristics and 165 rRNA PCR amplification.

A Study on the Characteristics of Natural Preservative Agent-treated Fabrics for Textile Cultural Properties Preservation (섬유문화재 보존에 활용하기 위한 천연보존제로 처리한 직물의 특성연구)

  • Baek, Young-Mee
    • Journal of Conservation Science
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    • v.25 no.2
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    • pp.197-206
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    • 2009
  • Natural preservative agents are generally made of antibiotic substances that are extracted from plants. They are used mainly to keep in an original good state food, natural cosmetic goods and medicines which are likely to get rotten. The purpose of this study is to investigate whether natural preservative agents can be applied to fabrics for the preservation of textile cultural properties. For the purpose, this researcher experimented with a certain natural preservative agent which was developed to preserve natural cosmetic goods. The study found that when treated with the foresaid natural preservative agent whose ratio to water was 1%, fabrics showed little changes in color and tensile strength, almost neutral pH, high antibiosis and anti-fungus and high resistance to Bacillus cereus that is much detrimental to silk fabrics. As a part of the study, a preliminary test on the possibility of using natural preservative agents to preserve textile cultural properties, found that when kept covered up with the 1% natural preservative agent-treated fabrics for 72 hours, excavated textile relics showed a dramatic decrease in microbial growth.

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Anti-acne and Anti-atopic Dermatitis Effect of Plant Extracts Including Eucommia ulmoides Oliv and Phellodendron amurense (두충나무, 황벽나무 등을 포함하는 수목추출물의 항여드름 및 항아토피 효과)

  • Kim, Gi Eun;Kim, Jin Hong;Hong, Seul Ki;Kim, Tagon;Kim, Donguk
    • Korean Chemical Engineering Research
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    • v.48 no.6
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    • pp.700-703
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    • 2010
  • In this study, plant extracts including Eucommia ulmoides Oliv. and Phellodendron amurense were studied to test possible application for cosmetics and skin related medicine. Anti-oxidation effect of plant extracts was measured by DPPH free radical scavenging activity and it was insignificant at low concentration, however, it was as good as vitamin C, excellent anti-oxidation agent, at $1000{\mu}g/ml$. Anti-bacterial effect was tested by disc diffusion method, and plant extracts showed mild anti-bacterial effect for normal skin flora, Staphylococcus epidermidis while it indicated strong anti-bacterial effect for acne inducing Propionibacterium acne. Therefore it had powerful potential for anti-acne material because of selectivity. Anti-atopic dermatitis effect was tested by hairless mouse and plant extracts recovered damaged skin to near normal condition after 14 days of treatment. IgE concentration in treated mouse was decreased 16% compared with control. From the research, plant extracts indicated strong anti-acne and anti-atopic dermatitis effect, and showed strong potential for cosmetics and skin related medicine.

Quality Characteristics and Functional Properties of Ethanol Extract of Propolis (Propolis 에탄올 추출물의 품질특성 및 기능성)

  • 서권일;오인석;오동환;최성희;손미예;문주석
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.6
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    • pp.969-972
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    • 2000
  • Quality characteristics and functional properties of four kinds of propolis collected from 4 different regions were compared. Ethanol extract of propolis (EEP) showed high absorbance of UV at 208 ~ 212 nm and at 292~294 nm. Total flavonoid contents of 4 propolis were at a range of 16.30~21.87 mg/g However, no significant difference in the flavonoid content was observed among four kinds of sample. All EEP showed the high antimicrobial activities, especially against Salmonella typhimurium. Hydrogen donating activities of the EEP were high appear 65.94 to 78.67% in all samples. The EEP from Sunchon showed the highest activity of hydrogen donation among samples. All extracts had the higher nitrite-scavenging activity than 0.1% BHT. Compared to control, a significant decrease in lipid oxidation was found in all 4 extracts.

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The Crack Resistance for PSG and Pe-Sin Films in the Semiconductor Device (반도체소자의 표면보호용 PSG, PE-SIN박막의 항균열특성에 대한 연구)

  • Ha, Jung-Min;Shin, Hong-Jae;Lee, Soo-Woong;Kim, Young-Wug;Lee, Jung-Kyu
    • Korean Journal of Materials Research
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    • v.3 no.2
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    • pp.166-174
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    • 1993
  • Abstract The crack resistance of PSG(Phosphosilicate Glass) and PE-SiN(Plasma Enhanced CVD S${i_2}{N_4}$)films deposited on aluminium thin films on Si substrate was analyzed in this study. PSG was deposited by AP-CVD and PE- SiN by PE-CVD. All the films underwent repeated heat cycles at 45$0^{\circ}C$for 30 min. Crack formation and development were examined between each heat cycle. The crack behavior was found to be closely related to the stresses in the films. The stress induced by the difference in thermal expansion behavior between the passivation layers and underlying aluminum film may cause the crack. Crack resistance decreases as the thickness of PSG films increases due to the high tensile stress of the films. Phosphorus in the PSG films releases tensile stress and consequently the stress of the films tends to show compressive stress. As a result, crack resistance increased as the concentratin of P in the PSG films increased. Crack resistance in the PE-SiN films also increased with compressive stress. An experimental model to predict crack generation in the PSG and PE-SiN films during heat cycle was suggested.

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Development of Fermented Isotonic Beverage with Anticariogenic Activity using Bacteriocin-Producing Lactic Acid Bacteria (박테리오신 생성 젖산균을 이용한 항충치 활성을 지닌 발효이온음료 개발)

  • Jung, Dong-Sun;Lee, Young-Kyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.3
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    • pp.399-404
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    • 2002
  • The fermented fruit and vegetable mixed broth was prepared by using bacteriocin-producing lactic acid bacteria to evaluate the possibility of developing isotonic beverage with anticariogenic activity. Optimum conditions were also established to produce bacteriocin by a mixed culture system consisting of Lc. lactis and Leu. mesenteroides in a fruit and vegetable mixture. Production of bacteriocin was not observed when both strains were simultaneously innoculated, but pH adjustment of the broth fermented by Leu. mesenteroides to nearneutral pH stimulated the production of bacteriocin by Lc. lactis. The concentration of sodium of the fermented broth was higher than those of commercial products. Color change of the fermented broth was not observed during storage. The fermented broth showed strong inhibitory effect against Streptococcus mutans which is an oral inhabitant with a cariogenic activity. Bacteriocin activity in the fermented broth was retained very stable for 4 weeks at 4$\^{C}$. The results indicated that bacteriocin-producing lactic acid bacteria can be used for the preparation of a Korean style thirst-quenching beverage containing bacteriocin.

Preparation and Properties of Antimicrobial Zinc Alginate Films according to Solution Concentration (용액 농도에 따른 항균성 알긴산 아연 필름의 제조 및 그 특성)

  • Lee, Ju-Hyun;Seo, Hye-Jin;Son, Tae-Won;Lim, Hak-Sang
    • Polymer(Korea)
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    • v.37 no.6
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    • pp.677-684
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    • 2013
  • Zinc alginate films were prepared by a film maker from sodium alginate solutions of different weight ratios and then they solidified into 3 wt% content $ZnCl_2$ coagulation solution and washed and dried at a $60^{\circ}C$ oven for 20 min. The characteristics were measured by several methods (antimicrobial activity, viscosity, FTIR, TGA, SEM, EDS, contact angle, tensile strength and solubility) and the film properties were investigated. The antimicrobial test showed that the zinc alginate films result in excellent antimicrobial activity in the two strains (Klebsiella pneumonia, Staphylococcus). The surface of zinc alginate film from the solution of 9 wt% sodium alginate showed more uniform shape than any other films and the cross-section were hard and rough when the films were well-solidified by the $ZnCl_2$ solution. The tensile strength of zinc alginate films increased along with the concentration of sodium alginate solution due to the cross-linking, and the initial thermal decomposition temperature increased gradually.