• Title/Summary/Keyword: 합성 작용

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Inhibitory Effect of Galangin from Alpinia officinarum on Lipopolysaccharide-induced Nitric Oxide Synthesis in RAW 264.7 macrophages (고량강으로부터 분리된 galangin의 RAW 264.7 세포주에서 LPS로 유도된 nitric oxide 생성 저해활성)

  • Lee, Hwa Jin
    • Korean Journal of Food Science and Technology
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    • v.46 no.4
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    • pp.511-515
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    • 2014
  • In a screen for plant-derived inhibitors of nitric oxide (NO) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophage cells, a flavonol isolated from the chloroform extract of Alpinia officinarum was isolated. The structure of the flavonol was found to be 3,5,7-trihydroxy-2-phenylchromen-4-one (galangin, GLG) by using spectroscopy. GLG exhibited an inhibitory effect ($IC_{50}$ value: $26.8{\mu}M$) on NO production in LPS-stimulated RAW 264.7 murine macrophage cells. Moreover, GLG suppressed expressions of inducible nitric oxide synthase (iNOS) protein and mRNA in a dose-dependent manner.

Quantitative structure-activity relationship of N-substituted phenyl 5-chloro-1,3-dimethylpyrazol-4-carboxamides (N-치환 phenyl 5-chloro-1,3-dimethylpyrazole-4-carboxamide의 정량적구조활성상관관계)

  • Kim, Yong-Whan;Park, Chang-Kyu
    • Applied Biological Chemistry
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    • v.35 no.5
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    • pp.382-388
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    • 1992
  • Mycelial growth inhibition activity of forty-one N-substituted phenyl 5-chloro-1,3-dimethylpyrazole-4-carboxamides against Rhizoctonia solani was analysed quantitatively by multiple regression analysis using physicochemical parameters of substituents as independent variables and $pEC_{50}$ as dependent variable. As a result, a quantitative structure-activity relationship was formulated using eight physicochemical parameters, which explains 83% of variance of the fungicidal activity. The most important parameter for the biological activity was log k', as related to the penetration and transport processes in the biological system. The activity also correlated with other hydrophobic parameters$({\pi}_2,\;{\pi}_3)$, an electronic parameter$({\Sigma}{\sigma})$, and steric parameters$(STERIMOL\;parameters\;L_3,\;L_4)$.

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Analysis of Initial Synchronization Performance in OFDMA/TDD Systems (OFDMA/TDD 시스템의 초기 동기 성능 분석)

  • Seung Young-Min;Kim Ki-Nam;Cho Sung-Joon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2006.05a
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    • pp.410-414
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    • 2006
  • In the present, Orthogonal Frequency Division Multiple Access (OFDMA) that wireless access scheme for high speed data transmission is noticed in mobile communication market and OFDMA/TDD scheme will be used combining Time Division Duplex (TDD) scheme based on OFDMA. The Base Station's receiver synchronizes the symbol timing to anyone user's symbol and the other user's symbols have some Symbol Timing Offset (STO). Linear phase shift is occurred by each user's STO in an OFDMA symbol and the Multiple Access Interference (MAI) caused by the summation of each user's linear phase shift degrades the performance of ranging code detection. In this paper, we analyze the ranging code detection performance for each users STO in OFDMA/TDD system. Simulation results show that the more users access and mobile speed increase, the more ranging code detection performance degrades.

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A Study of Earth Pressure and Deformation acting on the Flexible Wall in Soft Soil (연약지반 흙막이벽에 작용하는 토압 및 변위에 관한 연구)

  • Park, Yeong-Mog;Chung, Youn-In
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.7 no.3
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    • pp.215-222
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    • 2003
  • Recently the deep and large excavations are performed near the existing buildings in urban areas for the practical use of underground space. The earth pressure due to the excavation are varied according to the conditions of ground, the depth of excavation, the construction methods, and the method of supporting the earth pressure etc.. In this study, not only the behavior of axial load and distribution of earth pressure on the flexible wall according to stage excavation depth but also magnitude and distribution of lateral deformation, and the equivalent earth pressure from strut axial loads were analyzed by the results measured from instruments such as, load cells, strain gauges, and in-situ inclinometer, on the field of subway construction. According to the results of this study in the case of stage excavation the earth pressure of soft clayey soil is compounded with Terzaghi-Peck and Tschebotarioff.

Synthesis and Characterization of Non-Conjugated Polymers with Hole-Conductor and Red-Emitter in Side-Chain (정공 전달물질 및 적색발광 물질이 곁사슬에 포함된 비공액 고분자의 합성과 특성 분석)

  • Shim, Na-Young;Lee, Hoo-Sung
    • Polymer(Korea)
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    • v.29 no.5
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    • pp.486-492
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    • 2005
  • Into a no-conjugated polymer chain we have introduced side chains with a styrene-linked triphenylamine segment as a $\pi-electron$ donor, styrene-]inked aminobenzaldehyde segment as a tunable reactive -CHO group, and PM (4-(dicyanomethylene)-2-(tert-butyl)-4H-pyran) moiety as a $\pi-electron$ acceptor for red emitting materials. The thermal stability and the optical properties of the statistical copolymers have been studied. All the polymers were electrochemically active and showed electroluminescent emission at around 700nm. The EL device of P5-PM based on the sturcture of $ITO/PPV/polymer/BCP/Alq_3/Al$ showed a maximum brightness of $120cd/m^2\;at\;50mA/cm^2$ with an external quantum efficiency of $0.67\%$. It was possible to enhance the external quantum efficiency by balancing the charge recombination. A red-emitting polymer with high external quantum efficiency was developed by incorporating bifunctionality.

Optimal Culture Conditions for Transformed Root Growth and Trichosanthin Formation in Trichosanthes kirilowii Max. (하늘타리 형질전환근의 생장 및 Trichosanthin의 생합성을 위한 최적화)

  • Hwang, Sung-Jin;Na, Myung-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.1
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    • pp.46-50
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    • 2007
  • Transformed hairy roots were induced from in vitro grown plantlets of Trichosanthes kirilowii by infection with Agrobacterium rhizogenes strain ATCC15834. Transformed hairy roots exhibited active growth with high branching of roots on plant growth regulators-free medium. Cloned line (TR-03) of hairy root was tested for its growth and extracellular protein accumulation in medium under various culture conditions. Among the culture media tested, a full-strength MS medium had a pronounced effect on root biomass and extracelluar protein accumulation in medium. The maximum root biomass (2.4 g DRW/flask) and extracellular total protein contents $(28.3ug/m\ell)$ in medium was obtained at inoculum size of 2 g (FRW) and in MS medium supplemented with 4% sucrose. In addition, the optimal shaking speed for root growth and extracellular protein accumulation in medium were 100 rpm. The total extracellualr protein concentration reached a maximum of $28.3ug/m\ell$ at 4 weeks and decreased thereafter. Protein translation inhibitory activity was observed in culture broths and reached levels of 21.3 unit. These studies demonstrate that the transformed hairy roots can be utilized for the in vitro production of ribosome-inactivating proteins.

Levels of Resistance and Fitness in Glufosinate-ammonium-Resistant Transgenic Rice Plants (Glufosinate-ammonium 저항성 형질전환벼의 저항성 수준과 적응성에 관한 연구)

  • Yun, Young Beom;Kuk, Yong In
    • Weed & Turfgrass Science
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    • v.1 no.4
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    • pp.50-56
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    • 2012
  • The objectives of this research were to quantify resistance levels of transgenic rice expressing the bar gene to glutamine synthetase (GS)-inhibiting, and methionine sulfoximine and photosynthesis-inhibiting herbicide, paraquat, and compare the ammonium accumulation, chilling injury, and yield between transgenic and non-transgenic rice. The transgenic rice lines were 45-96-fold more resistant to glufosinate-ammonium than non-transgenic rice. The transgenic rice lines were also 18-fold more resistant to methionine sulfoximine, but was not resistant to paraquat, which has different target site. Glufosinate-ammonium increased the ammonium accumulation in leaves of non-transgenic rice plants, but had minimal or no effect on leaves of transgenic lines. The transgenic lines except for 258, 411, 607 and 608 were more susceptible during chilling and recovery than non-transgenic rice plants. The yield of transgenic lines 142, 144, 258 and 608 was similar or higher than that of non-transgenic rice in pot conditions.

Reaction Mechanism of Acetylcholinesterase Catalyzed Hydrolysis of Thiocholine Esters (아세틸콜린에스터라제 촉매에 의한 티오에스테르의 가수분해 반응 메카니즘 연구)

  • 정대일;최순규;이용균;박유미;곽문정;신영주;최병욱;이봉호;한정태
    • Journal of Life Science
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    • v.12 no.1
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    • pp.32-42
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    • 2002
  • It was researched to react horse serum-BChE with hefanoylthiocholine chosen among choline esters. According as number of carbon of acyl group in choline esters was increased, reactivity was decreased but strength of ES complex was increased (Km=0,140mM). The pH-V/K profile for BChE-catalyzed hydrolysis of hexanoylthiocholine yields a p $K_{a}$ =4.974$\pm$0.028. This value is equal to recent literature that shows systematic shift from dependence of activity on the basic form fo a residue that huts a p $K_{a}$ =6.2~6.4 to catalysis by a residue or residues that has a p $K_{a}$ =4.7~5.0. The resulting kinetic solvent isotope effect of hexanoylthiocholine is $^{D/V}$K=1.18. The magnitude of the isotope effect suggests that proton transfer is not an element of transition-state stabilization.n.

Antioxidant Activity of Different Lipid Extracts from Mackerel Viscera (고등어 내장으로부터 추출한 지질성분의 항산화효과)

  • 변대석;정용실;홍정화
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.1
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    • pp.98-104
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    • 1995
  • To utilize the mackerel viscera effectively, antioxidant substances were extracted and the effects on the oxidation were evaluated. Total lipid(TL) extracted from mackerel viscera were fractionated into hexane extracts, tetracarbon chloride($CCl_4$)-methanol(1 : 1, 1 : 3, 1 : 9, v/v) extracts, phospholipid(PL), phosphatidylcholine(PC), and PC free fracitons(PCF). Inhibition of fish oil oxidation was investigated using these fractions. Hexane fraction showed lower antioxidant activity compared to the TL or $CCl_4-methanol$ fractions(C-M) ; in addition, antioxidant activities increased as polarity of C-M increased. Significant antioxidant activities were observed with PL, PC, and PCF ; among them, PC was the most effective. To determine the antioxidant activity of PC, effects of natural or synthetic antioxidant were compared. Addition of PC to fish oil proved to enhance antioxidant activity the antioxidant activity of phospholipids was derived mainly from PC.

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Some Applications of SAR Imagery to the Coastal Waters of Korea (한국 주변 해역에서의 SAR 영상 응용예)

  • 김태림
    • Korean Journal of Remote Sensing
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    • v.15 no.1
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    • pp.61-71
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    • 1999
  • Several physical phenomena on the sea surface are analyzed from SAR images of South Sea areas, Korea. Strong wave patterns propagating in southerly direction are seen in ERS-1 SAR image on October 11, 1994, and a wave directional spectrum is calculated from this image using the SAR modulation transfer function. RADARSAT SAR image of August 15, 1996 reveals internal waves in northern coastal waters of Cheju Island. Analysis indicates that the internal waves may have been generated by the tidal currents traveling over the shallow bottom of the stratified water in the summer during the tidal changeovers fro ebb to flood and shows patterns of trains of solitons. RADARSAT SAR image taken 3 days after the oil spill accident near Goeje Isalnd on April 3, 1997 detects distinct oil slicks from the accident area but also shows slicks near the coast caused by wind sheltering of coastal mountains and chemical-biological activities.