Weed & Turfgrass Science

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Levels of Resistance and Fitness in Glufosinate-ammonium-Resistant Transgenic Rice Plants

Glufosinate-ammonium 저항성 형질전환벼의 저항성 수준과 적응성에 관한 연구

  • Yun, Young Beom (Dept. of Development in Oriental Medicine Resources, College of Life Science and Natural Resources, Sunchon National University) ;
  • Kuk, Yong In (Dept. of Development in Oriental Medicine Resources, College of Life Science and Natural Resources, Sunchon National University)
  • 윤영범 (순천대학교 생명산업과학대학 한약자원개발학과) ;
  • 국용인 (순천대학교 생명산업과학대학 한약자원개발학과)
  • Received : 2012.11.23
  • Accepted : 2012.12.09
  • Published : 2012.12.31
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Abstract

The objectives of this research were to quantify resistance levels of transgenic rice expressing the bar gene to glutamine synthetase (GS)-inhibiting, and methionine sulfoximine and photosynthesis-inhibiting herbicide, paraquat, and compare the ammonium accumulation, chilling injury, and yield between transgenic and non-transgenic rice. The transgenic rice lines were 45-96-fold more resistant to glufosinate-ammonium than non-transgenic rice. The transgenic rice lines were also 18-fold more resistant to methionine sulfoximine, but was not resistant to paraquat, which has different target site. Glufosinate-ammonium increased the ammonium accumulation in leaves of non-transgenic rice plants, but had minimal or no effect on leaves of transgenic lines. The transgenic lines except for 258, 411, 607 and 608 were more susceptible during chilling and recovery than non-transgenic rice plants. The yield of transgenic lines 142, 144, 258 and 608 was similar or higher than that of non-transgenic rice in pot conditions.

본 연구의 목적은 bar 유전자를 발현한 형질전환벼에 대한 글루타민 합성효소 저해제인 glufosinate-ammonium, L-metionine sulfoximine과 광합성저해제인 paraquat에 저항성 수준과 형질전환벼와 비형질전환벼의 암모늄 축적량, 저온반응 및 수량을 비교하는데 있다. 형질전환벼 계통은 비형질전환벼에 비해 glufosinate-ammonium에 45~95배 높은 저항성을 보였다. 한편 형질전환벼 계통은 L-metionine sulfoximine을 처리했을 때도 형질전환벼 계통이 비형질전환벼에 비해 18배 이상의 저항성을 보여 교차저항성이 있음을 확인할 수 있었다. 그러나 glufosinate-ammonium과 작용기작이 다른 제초제인 paraquat에 대한 다중저항성은 없었다. Glufosinate-ammonium 처리에 의해 ammonium 축적은 비형질전환벼에서 증가하였으나 모든 형질전환벼의 계통에서는 적거나 효과가 없었다. 형질전환벼 계통 258, 411, 607 및 608을 제외한 계통은 비형질전환벼에 비해 저온 내성 및 저온 후 회복정도가 유의적으로 낮은 경향을 보였다. 형질전환벼 계통 142, 144, 258 및 608의 수량은 비형질전환벼와 유사하거나 높았다.

Keywords

References

  1. Cao, M.X., Huang, J.Q., Wei, Z.M., Yao, Q.H., Wan, C.Z. and Lu, J.A. 2004. Engineering higher yield and herbicide resistance in rice by Agrobacterium-mediated multiple gene transformation. Crop Sci. 44:2206-2213. https://doi.org/10.2135/cropsci2004.2206
  2. Coetzer, E. and Al-Khatib, K. 2001. Photosynthetic inhibition and ammonium accumulation in Palmer amaranth after glufosinate application. Weed Sci. 49:454-459. https://doi.org/10.1614/0043-1745(2001)049[0454:PIAAAI]2.0.CO;2
  3. D'Halluin, K., De Block, M., Janssens, J., Leemans, J., Reynaerts, A. and Botterman, J. 1992. The bar gene as a selectable marker in plant engineering. Method. Enzymol. 216:415-441. https://doi.org/10.1016/0076-6879(92)16038-L
  4. De Block, M., Botterman, J., Vandewiele, M., Dockx, J., Thoen, C., Gossele, V., Rao Mowa, N., Thompson, C., Van Montaqu, M and Leemans, J. 1987. Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J. 6:2513-2518.
  5. De Block, M., De Brouwer, D. and Tenning, P. 1989. Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol. 91:694-701. https://doi.org/10.1104/pp.91.2.694
  6. Jeong, E.G., Yi, G.H., Won, Y.J., Park, H.M., Cheon, N.S., et al. 2005. Agronomic characteristics of transgenic japonica rice "Milyang 204" with herbicide resistance gene (bar). Korean J. Plant Biotechnol. 32(2):85-90. (In Korean) https://doi.org/10.5010/JPB.2005.32.2.085
  7. Krausz, R.F., Kapusta, G., Matthews, J.L., Baldwin, J.L. and Maschoff, J. 1999. Evaluation of glufosinate-resistant corn (Zea mays) and glufosinate: Efficacy on annual weeds. Weed Tech. 13:691-696. https://doi.org/10.1017/S0890037X00042093
  8. Kuk Y.I., Kim, K.H., Kwon, O.D., Lee, D.J., Burgos, N.R., Jung. S. and Guh, J.O. 2003. Cross-resistance pattern and alternative herbicides for Cyperus difformis resistant to sulfonylurea herbicides in Korea. Pest Manag. Sci. 60:85-94.
  9. Kuk, Y.I., Burgos, N.R. and Shivrain, V.K. 2008. Natural tolerance to imazethapyr in red rice (Oryza sativa). Weed Sci. 56:1-11. https://doi.org/10.1614/WS-07-073.1
  10. Manderscheid, R. and Wild, A. 1986. Studies on the mechanism of inhibition by phosphinothricin of glutamine synthetase isolated form Triticum aestivum L. J. Plant Physiol. 123:135-142. https://doi.org/10.1016/S0176-1617(86)80134-1
  11. Manickavasagam, M., Ganapathi, A., Anbazhagan, V.R., Sudhakar, B., Selvaraj, N., Vasudevan, A. and Kasthurirengan, S. 2004. Agrobacterium-mediated genetic transformation and development of herbicide-resistant sugarcane (Saccharum species hybrids) using axillary buds. Plant Cell Rep. 23:134-143.
  12. Oard, J.H., Linscombe, S.D., Braverman, M.P., Jodari, F., Blousin, D.C., Leech, M., Kohli, A., Vain, P., Cooley, J.C. and Christou, P. 1996. Development, field evaluation, and agronomic performance of transgenic herbicide resistant rice. Mol. Breed. 2:359-368. https://doi.org/10.1007/BF00437914
  13. Park, H.M., Kim, Y.H., Suh, J.P., Choi, M.S., Kim, K.J., Shin, D.B., Park, C.H. and Lee,J.Y. 2007. Evaluation of agronomic and molecular biological characteristics of the herbicide resistance transgenic rice. Korean J. Breed. Sci. 39(2):148-154. (In Korean)
  14. Pesticide Use Manual. 2012. Korea Crop Protection Association. p. 1308. (In Korean)
  15. Petersen, J. and Hurle, K. 2001. Influence of climatic conditions and plant physiology on glufosinate-ammonium efficacy. Weed Res. 41:31-39. https://doi.org/10.1046/j.1365-3180.2001.00214.x
  16. Shelp, B.J., Swanton, C.J., Mersey, B.G. and Hall, J.C. 1992. Glufosinate (phosphinothricin) inhibition of nitrogen metabolism in barley and greenfoxtail plants. J. Plant Physiol. 139:605-610. https://doi.org/10.1016/S0176-1617(11)80378-0
  17. Singh, R.J. and Khush, G.S. 2000. Cytogenetics of rice. In: Nanda, J.S. (Ed.), Rice Breeding and Genetics-Research Priorities and Challenges. Enfield, NH: Science Publishers, pp. 287-311.
  18. Strauch, E., Wohlleben, W. and Puhler, A. 1988. Cloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes TU494 and its expression in Streptomyces lividans and Escherichia coli. Gene 63:65-74. https://doi.org/10.1016/0378-1119(88)90546-X
  19. Wild, A. and Manderscheid, R. 1984. The effect of phosphinothricin on the assimilation of ammonia in plants. Z. Naturforsch. 39c:500-504.
  20. Wild, A. and Wendler, C. 1993. Inhibitory action of glufosinate on photosynthesis. Z. Naturforsch. 48c:367-373.
  21. Yi, G., Shin, Y.M., Choe, G., Shin, B., Kim, Y.S. and Kim, K.M. 2007. Production of herbicide-resistant sweet potato plants transformed with the bar gene. Biotechnol. Lett. 29:669-675. https://doi.org/10.1007/s10529-006-9278-1