• 생약학회지
• /
• 제54권1호
• /
• pp.38-43
• /
• 2023

A simple and accurate assay was developed for the quantitative analysis of 1-deoxynojirimycin (1-DNJ) derived from the silkworm (Bombyx mori). Normal-phase high-performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD) and a hydrophilic interaction liquid chromatography column was used. Various parameters were applied to optimize the analysis method. The limits of detection and quantification of 1-DNJ were 2.97 × 10^-3 and 9.00 × 10^-3 mg/mL, respectively. The calibration curve showed good linearity results. The concentration range and the r² value were 0.0625-1.0 mg/mL and 0.9997, respectively. The accuracy test demonstrated a significantly high recovery rate (89.95-103.22%). The relative standard deviation was ≤ 1.00%. Thus, a method for the accurate identification and quantitative analysis of 1-DNJ in silkworms was developed. Moreover, in this procedure, the process of derivatization of 1-DNJ, which was required in previous experiments, could be eliminated. This technique may be actively utilized for the development of pharmaceuticals and health functional foods using 1-DNJ.

• 한국식품영양학회지
• /
• 제36권6호
• /
• pp.445-451
• /
• 2023

This study investigated the antioxidant activities of wine made with aronia (Aronia melanocarpa). The ethanol concentration of the aronia wine was increased up to 7.8±0.1% on the 8th day of fermentation. Compared to other types of wine, the total amount of organic acids was highest in raspberry wine, followed by grape wine, arona wine, and aronia juice. Because, acetic, oxalic, and succinic acids were not detected in the aronia juice, but were detected in the aronia wine, it was determined that they were produced during alcohol fermentation. The polyphenol content in the aronia juice and wine was higher than in the grape wine and raspberry wine and was twice as much in the aroni wine than in the aronia juice. The flavonoid content in aronia juice and wine was higher than in commercial grape wine and raspberry wine. The DPPH radical scavenging ability was higher than 50% in the aronia wine and juice samples. ABTS radical scavenging activity was higher in aronia juice and wine than in raspberry wine and grape wine. The results of this study suggest that the development of wine with high antioxidant activity is possible if wine is made with aronia.

• 정보보호학회논문지
• /
• 제18권6A호
• /
• pp.27-37
• /
• 2008

일반서명은 누구나 서명의 정당성을 검증 가능하기 때문에 메시지에 대한 인증을 제 3자에게 전가함으로서 프라이버시 침해를 일으키기도 한다. 이에 Jakobsson 등은 지정된 사람만이 서명을 검증할 수 있게 하는 지정된 검증자 서명기법을 처음 소개 하였다. 지정된 검증자 서명기법은 오직 선택된 검증자만이 서명을 검증할 수 있게 하는 기법으로 메시지 인증성질의 문제점과 프라이버시 침해를 해결한다. 본 논문에서는 '인증서가 없는(Certificateless)' 개념을 기반으로, 서명자의 프라이버시를 강화한 인증서 없는 강한 지정된 검증자 서명기법을 제안하고자 한다. 제안하는 기법은 기존의 인증서 없는 지정된 검증자 서명기법에 비해 다소 효율성이 떨어지나 강한의 성질과 양도성의 문제를 가지지 않는 인증서가 없는 강한 지정된 검증자 서명 기법은 본 논문이 최초이다.

• 한국식품영양학회지
• /
• 제33권6호
• /
• pp.639-644
• /
• 2020

The purpose of this study was to investigate the quality characteristics of frankfurter sausage made with venison. The crude protein content of sausage showed a significant increase pattern in the proportion to the addition of the venison. The crude fat content showed a decrease pattern. The saturated fatty acid significantly increased in proportion to the content of the venison. The monounsaturated fatty acids and the polyunsaturated fatty acids decreased with increasing the venison content. The content of the free amino acids increased in proportion to the amount of the venison added. The amino acid content of the pork sausage was in the order of Alanine > glycine > glutamic acid, and was similar in the sausages made from venison. The essential amino acids were 36.4% in FSV, higher than 34.0% in the pork sausages. In the sensory test, it was confirmed that the texture, taste, and overall acceptability increased with the addition of the venison. Because the sausages made from the venison were superior to the pork sausages in all of the measured sensory characteristics, it is considered that industrialization is sufficiently feasible.

• 대한본초학회지
• /
• 제29권5호
• /
• pp.31-38
• /
• 2014

Objectives : Egg yolk is composed of various important chemical substances for human health. A calcium shortage causes the growth retardation on the body growth. In this study, we examined the therapeutic effects of calcium, vitamin D and egg yolk peptide (EYP) treatment on the retardation of the longitudinal bone growth induced by low-calcium diet in adolescent rats. Methods : Low calcium diets were administrated for 15 days. During the last five days, calcium and/or vitamin D and/or EYP were administrated. The body weights, longitudinal bone growth rates, the heights of growth plates, and bone morphogenetic protein (BMP)-2 and insulin-like growth factor (IGF)-1 expressions were measured using histochemical analysis. Results : Low calcium diets caused the significant reduction in body weight gains and the longitudinal bone growth. The heights of growth plates and the expressions of BMP-2 and IGF-1 showed the impairment of body growth as well. Calcium and/or vitamin D administration could not significantly increase the longitudinal bone growth. However, calcium, vitamin D, and EYP administration significantly increased the bone growth, the growth plate height, and BMP-2 and IGF-1 expressions. Conclusions : These results suggest that EYP enhances the longitudinal bone growth in the calcium and/or vitamin D deficiency and it could be a promising agent for the treatment of children suffering from malnutrition.

• 환경위생공학
• /
• 제8권2호
• /
• pp.155-168
• /
• 1993

The chemical components of rain water and aerosol in Bulkwang of Seoul and Yang-Paying of Kyunggi-do were compared in order to investigate that pollutants at urban area influences rain water and aerosol component. The following is the result of this study. 1) The pH of rain water was shown 4.82 at urban area and 5.69 at suburban area. 2) The mean concentration of $SO_4^{2-}$ within rain water at suburban was nearly the same at urban area but the mean concentration of $Na^{+},Ca^{2+},Mg^{2+}$ and $NH_4^{2+}$ at suburban area was higher than the corresponding levels at urban area. 3) The ion component of aerosol at suburban was shown 68% of that at urban area. This study proved that the concentration of rain water and aerosol component was under the influence of movement of air current.

• 환경위생공학
• /
• 제9권2호
• /
• pp.101-109
• /
• 1994

Acute toxicity of heavy metals( mercury, cadmium lead, chromium and copper ), organophosphours insecticides( EPN, 2, 4- D, and parathion ), and other chemicals( arsenic, cyanide, and phenol ) to Daphnia magna was analyzed. Acute toxicity of heavy metals, organophosphours insecticides and other chemicals on Daphnia magna, LC$_{50}$ was showed 0.07-0.73mg/1, 0.22-1.94mg/t and 1.73-39.92mg/1 respectively. Daphnia magna to most of toxic chemicals was sensitive and then it seems to be useful except phenol in toxic test of water. Toxic chemicals were classified as Group 1 which had a high LC$_{50}$ value and the low increase rate of toxicity according to the increase of concentration, Group 2 which had a high LC$_{50}$ value and the high increase rate of toxicity, Group 3 which had a low LC$_{50}$ value and the low increase rate of toxicity. To Daphnia magna, lead, chromium, EPN, and parathion were included in Group 1; Mercury and copper in Group 2; Arsenic and cadmium in Group 3; Cyanide 2,4- D, and phenol in Group 4.

• 센서학회지
• /
• 제28권3호
• /
• pp.171-176
• /
• 2019

The biomarkers related to the colorectal cancers and diseases were surveyed and summarized, and an optical electronic nose was researched and developed for their analysis. The prototyped sensor revealed that it could discriminate two gases: ethanol 2000 ppm and $CO_2$ 500 ppm. Furthermore, the sensor demonstrated the potential capability of estimation of $CO_2$ concentration with 95% confidence level. Based on the above experimental results, the developed optical electronic nose was tested with the mixtures of gases (Isopropyl Alcohol, Acetone, Methanol, and Toluene) and the biomarkers were successfully segregated using principal component analysis.

• 대한의료기공학회지
• /
• 제22권1호
• /
• pp.83-96
• /
• 2023

Objective : The purpose of this study is to report that patients with panic disorder were able to discontinue the psychotropic medication through Korean Medicine treatment alone. Methods : The patient diagnosed with panic disorder were hospitalized on the 9th and were treated steadily through outpatient treatment afterwards with herbal medicine, acupuncture, cupping therapy, and Whidam's Sugi therapy while taking the chemical. Treatment effects were evaluated through BAI, BDI, STAI-X-1, CSEI-s, GIS, NDI-K, and EQ-5D measurements. Results & Conclusions : The patient's anxiety was relieved and the psychotropic medication was discontinued, but it did not recur. And after inpatient treatment, he has been steadily receiving outpatient treatment on a regular basis, maintaining an improved state and leading a normal daily life.

• 생명과학회지
• /
• 제29권11호
• /
• pp.1192-1199
• /
• 2019

본 연구는 화장품 소재로서 우절 에탄올추출물의 가능성을 확인하기 위한 것이다. 이를 위해 우리는 연근의 마디부분을 70%에탄올로 추출하여 사용하였으며 미백효능 및 주름개선 효과에 대한 생물학적 활성 평가를 수행하였다. 시료의 미백활성을 알아보기 위해 멜라닌세포(B16F10 cells)의 MTT assay를 이용한 샘플의 독성평가와 멜라닌 생성에 영향을 주는 관련 단백질의 발현량을 확인하였다. 시료의 주름활성억제 평가를 위해 collagenase inhibition assay를 수행하였다. 또한 UVB로 유도된 섬유아세포(CCD-986sk cells)의 MTT assay를 이용한 샘플의 독성평가와 matrix metalloprotease (MMP-1) inhibition 및 procollagen synthesis를 평가하였다. 미백활성 평가를 알아보기 위한 western bolt 결과, 멜라닌 생성과 관련된 두 단백질 Tyrosinase related protein-1 (TRP-1, Tyrosinase related protein-2 TRP-2)의 합성이 농도의존적으로 감소됨을 나타내었다. 게다가 우절 에탄올추출물의 collagenase inhibiion 실험의 결과, $500{\mu}g/ml$의 농도에서 대조군인 EGCG와 효능이 비슷한 80% 이상의 효과를 나타내었다. Procollagen synthesis 결과 UVB 자극군에 의해 콜라겐 합성은 68.8%로 감소되었으며 시료의 $25{\mu}g/ml$의 농도에서 80.2%의 콜라겐 합성에 회복능을 보였다. MMP-1 inhibition 실험결과는 $25{\mu}g/ml$ 농도에서 20.2%의 저해능을 나타내었다. 실험 결과는 NRN의 미백 및 주름 억제 효과를 검증하고, 향후 안전한 천연 화장품 재료로 사용될 수 있음을 확인했다.