• Korean Journal of Poultry Science
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• v.35 no.4
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• pp.361-366
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• 2009

The purpose of this study was to assess the genetic variation and establish the relationship amongst breeds and strains using 7 chicken specific microsatellite markers. A total of 317 DNA samples from four Korean native chicken (KNC) strains (KR: Korean Native Red chicken strain, KY: Korean Native Yellow chicken strain, KL: Korean Native Black chicken strain, KO: Ogol chicken strain) and three introduced endemic chicken breeds (LE: Leghorn chicken breed, RI: Rhode Island Red chicken breed, CO: Cornish chicken breed). The size of microsatellite markers was decided using GeneMapper Software (v.4.0) after being analyzed using an ABI 3130 Genetic Analyzer. Frequencies of microsatellites markers were used to estimate heterozygosities and genetic distances. The lowest distance (0.074) was observed between the KY and KL breeds and the highest distance (0.779) between the KL and LE breeds. The KNC strains (KR, KY, KL) have comparatively near genetic distance each other. On the other side, each individual was not ramified to different groups and were spread evenly in phylogenetic dendrogram about all the KNC of each strain populations. But the endemic breed populations (LE, RI, CO) were ramified to different groups. The microsatellite polymorphism data were shown to be useful for assessing the genetic relationship between Korean native strains and other foreign breeds.

• Korean Journal of Poultry Science
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• v.37 no.3
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• pp.247-253
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• 2010

Telomeres are nucleoprotein structures at the ends of chromosomes consisting of DNA sequences arranged in tandemly repeated units $(TTAGGG)_n$. However, this hexamers can also occur at non-telomeric sites in some avians and vertbrate. This study was carried out to present the distribution of telomeric DNA sequences in Korean Native Chicken chromosomes. The fluorescence in situ hybridization technique using a telomeric DNA probe was performed to the metaphase spreads of chicken early embryonic cells. Telomeric DNA signals were detected at the ends of chromosomes including macrochromosomes and microchromosomes. In chicken, surprisingly, chromosome 1 showed very distinct interstitial telomeric DNA hybridization patterns which located two interstitial sites in the p-arm at 1p11 and 1p23, and one in the q-arm at 1q32. In chromosome number 2 and 3 also displayed interstitial telomeric signals (ITS) in the long arms at 2q24 and 3q32, respectively. The pattern of telomeric DNA distribution in Korean Native Chicken chromosomes was in agreement with a previously reported in Gallus domesticus. The relative amount of telomeric DNA sequences in each macrochromosomes ranged from 4.6% to 16.3%. Distribution of telomeric DNAs at the end of p-arm was much more than that of q-arm in almost chicken chromosomes. The distribution of ITS in chicken chromsomes implicate to tandem chromosome fusions that might have occurred during the process of karyotype evolution.

• Korean Journal of Poultry Science
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• v.37 no.1
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• pp.81-87
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• 2010

To estimate the genetic characteristics and cumulative power of discrimination (CPD) Korean Native Chicken. We used a total of 195 genomic DNAs from four breeds population (Korean Native Red chicken: R, Korean Native Yellow chicken: Y, Korean Native Black chicken: L, Ogal chicken: S). Frequencies of microsatellites markers were used to estimate heterozygosities and genetic distances. The lowest distance (0.05) was observed between the R and L strains and the highest distance (0.158) between the L and S strains. Korean native chicken strains (R, Y, K) have each other comparatively near genetic distance. Cumulative power of discriminate (CPD) was 99.999% by including the 10 microsatellites loci individual identification system. And then matching probability in that two different individuals incidentally have same genotype was estimated to $0.36{\times}10^{-7}$. The system employing the 10 markers therefore provided to be applicable to individual identification in Korea native chicken.

• Korean Journal of Poultry Science
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• v.35 no.4
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• pp.399-405
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• 2009

Two experiments were conducted to establish the basic data of feeding system in cross-bred Korean native chicks. A total of seven hundred twenty chicks were replaced the 36 floor pen for the first five weeks with $3{\times}3$ factorial design in Experiment 1. Four hundred eighty of five weeks old chicks were raised from six to ten weeks of age with $3{\times}2$ factorial design in Experiment 2. Dietary ME and CP were formulated to contain 3,000, 3,050, 3,100 kcal/kg and 21, 22, 23%, respectively in Experiment 1 and 3,050, 3,100, 3,150 kcal/kg and 18, 19% in Experiment 2. Weight gain, feed intake were measured and calculated the feed conversion. Blood were collected and analyzed at the end of experiments. In Experiment 1, weight gain showed significantly higher in 3,050, 3,100 kcal/kg treatments than 3,000 kcal/kg treatment (P<0.05), but was not different in CP treatments. Feed intake was statistically high in 3,000 kcal/kg treatment compared with 3,050 and 3,100 kcal/kg ones (P<0.05), and more increased in 21% CP treatments compared to that of 22 and 23 CP treatment (P<0.05). Feed conversion of birds fed 3,050 and 3,100 kcal/kg diet showed much lower than 3,000 kcal/kg treatments (P<0.05). FCR was signicantly improved (P<0.05) in chicks fed diets containing 21 and 22% CP as compared to that fed 20% CP. Blood protein, glucose, and total cholesterol tended to increase in high energy and diet treatments. Blood HDL was increased as dietary energy increased, whereas LDL increased in low CP treatments. In Experiment 2, weight gain was not consistent between treatments, but more increased in 18% CP treatments compared to that of 19% CP treatment from six to ten weeks old in cross bred chicks (P<0.05). Feed intake was similar to the result of weight gain, but more increased in 19% CP treatment than 18% CP treatment (P<0.05). There were no statistically difference in FCR, but seemed to improve as dietary ME increased. Blood total protein and glucose increased as dietary CP was high, but triglyceride and HDL increased in high versus low ME (P<0.05). The results of these experiments suggested that optimum dietary ME and CP, were 3,050, 3,150kcal/kg and 22, 19% for the first five weeks and second one, respectively.

• Korean Journal of Poultry Science
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• v.25 no.3
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• pp.137-145
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• 1998

This study was conducted to compare the meat characteristics Korean Native Commercial Chicken(KNCC) by feeding system Feeding system of T$_1$ and T$_2$were same types from hatch to 8 weeks, starter diets( 0~4 weeks, mash, ME 3,l00kcal, CP 22.94%), grower diets(4~8 weeks, crumble, ME 3,l00kcal, CP 19.31%). Nutrient contents of finisher diets of T$_1$(pellet, M.E 3, 200kcal, CP 20.44%) was higher than T$_2$(mash, ME 3,l00kcal, CP 14.88%). Breast and legs meat of KNCC were used to analyze chemical composition, physicochemical characteristics, textural traits and sensory evaluation test. Live weight(L.W) /abdominal fat accumulation(A.F.A) was significantly different(P<0.05), but live weight(L.W) /dressed weight(D.W), percentage of cutted meat, chemical composition(moisture, CF, EE, CA), meat color(CIE), physicochemical properties, sensory evaluation score were not significantly by feeding systerns, at 12 ar'd 16 weeks of age.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2004.11a
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• pp.97-98
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• 2004

The observed means and standard divisions of the major laying traits per generation in korean native chickens were as follow. The age at first egg, the egg weight at the first egg, the weight at 270days and the egg production to 270days were 147.1$\pm$1.9 days. 32.3$\pm$0.9 g, 49.4$\pm$0.7 g and 76.2$\pm$1.7 eggs. The regression coefficients were -0.75$\pm$0.01 days, 0.33$\pm$0.08 g, 0.19$\pm$0.05 g and 0.47$\pm$0.3 eggs respectively.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2004.11a
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• pp.99-100
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• 2004

The observed means and standard divisions of the egg shape per generation in korean native chickens were as follow. The egg major axis, the egg miner axis and the egg shape index were 53.0$\pm$0.6～55.6$\pm$0.8 mm, 39.1$\pm$0.8～40.9$\pm$0.2 mm and 72.7$\pm$1.5～75.9$\pm$0.5 mm. The regression coefficients were -0.02～0.20 mm, 0.10～0.52 mm and 0.19～1.00 mm respectively.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2004.11a
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• pp.101-102
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• 2004

The observed means and standard divisions of the egg texture per generation in korean native chickens were as follow. The egg shell strength, the egg shell thickness and the egg shell colour were 3.8$\pm$0.2~4.2$\pm$0.1 kg/$\textrm{cm}^2$, 356.4$\pm$4.9~368.8$\pm$6.5${\mu}{\textrm}{m}$ and 47.0$\pm$1.7~50.0$\pm$3.3％. The regression coefficients were -0.03~0.03 kg/$\textrm{cm}^2$, -0.84~4.40${\mu}{\textrm}{m}$ and -0.88~-0.36％ respectively.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.115-116
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• 2003

The significant strain effects were investigated In all the major economic traits in Korean native chicken. The highest Least Square(LS) means of the body weights at 150 and 270days of age were observed in Black strain as 1, 594.38 and 1, 911.57 g. The earliest LS mean of the ages at first egg was 146.88 days in White strain. The heaviest LS means of egg weights at the first egg and 270 days of age were observed in Red brown strain as 33.20 and 50.74 9. The LS mean of the largest number of egg production was 79.50 eggs in Yellow strain.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.127-128
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• 2003

This study was conducted to obtain for genetic information with 11, 538 progenies of 5 strains in Korean native chicken. The heritability estimates of the body weights at 150 and 270days of age were 0.25~0.61 and 0.32~0.59 in 5 strains, the heritabilities of the body weight at 270 days were relatively higher than that of the body weight at 150 days. The medium or low heritability estimates were observed in the ages at first egg as 0.15 ~0.31, and heritability estimates of the egg weights at the first egg and 270 days of age were 0.06~0.09 and 0.24~0.41. Medium or low heritability estimates were observed for the number of egg production as 0.09 ~0.26, respectively.