• Title/Summary/Keyword: 한국인 무릎 관절 모델

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Anti-osteoarthritis effect of Boswellia serrata gum resin extract in monosodium iodoacetate-induced osteoarthritic Sprague-Dawley rats (Monosodium iodoacetate 유도 골관절염 동물모델에서 보스웰리아 검레진 추출물의 항골관절염 효과 연구)

  • Jae In Jung;Ryong Kim;Eun Ji Kim
    • Journal of Nutrition and Health
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    • v.56 no.3
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    • pp.231-246
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    • 2023
  • Purpose: The aim of this study was to investigate the anti-osteoarthritic effect of the ethanol extract of Boswellia serrata gum resin (FJH-UBS) enriched with keto-β-boswellic acid and 3-O-acetyl-11-keto-β-boswellic acid compared to the conventional Boswellia serrata extract by adding the process of removing oil with hexane, in the monosodium iodoacetate (MIA)-induced osteoarthritis rat model. Methods: Sprague-Dawley (SD) rats were orally administered 0, 40, or 80 mg of FJH-UBS/kg body weight (BW)/day for 5 weeks and injected with MIA intra-articularly into right knee joints on day 15 to induce osteoarthritis. Changes in the knee joint microarchitecture, cartilage degradation, the expression of inflammatory mediators, cytokines, and matrix metalloproteinases (MMPs) in serum and synovia were observed. Results: Oral administration of FJH-UBS (80 mg/kg BW/day) reduced MIA-induced knee swelling and cartilage degradation and increased the expression of type II collagen and aggrecan in articular cartilage. Furthermore, FJH-UBS administration reduced MIA-induced increases in the serum levels of prostaglandin E2, leukotriene B4, interleukin (IL)-1β, IL-6, and MMP-13, and MIA-induced increases in the mRNA expressions of inducible nitric oxide synthase, cyclooxygenase-2, 5-lipoxygenase, IL-1β, IL-6, TNF-α, MMP-2, MMP-9, and MMP-13 in the synovia of knee joints. Conclusion: These results indicate that FJH-UBS exerts its anti-osteoarthritic effects by suppressing the expressions of inflammatory cytokines and MMPs and, thus, cartilage degradation. Furthermore, they suggest that FJH-UBS has potential use as a functional food that improves joint and cartilage health.

A Statistical Analysis of Joint Moments Acting on Men Performing a Seated Dynamic Task (앉은 자세에서 동적 작업을 수행할 때 작용하는 관절 모멘트의 통계학적 해석)

  • Jung, Ho-Il;Son, Kwon
    • Progress in Medical Physics
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    • v.2 no.2
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    • pp.161-173
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    • 1991
  • A statistical approach was carried out to analyze joint moments acting on the six subjects performing a right-handed seated task. The dynamic task analyzed consisted of moving a hand-held weight of lkg mass back and forth in front of a subject's chest at the shoulder level in an upright seated position. We used experimental data obtained in the Biomechanics Laboratory of the University of Michigan. Based on the acquired data from three trials by each subject, moments were calculated using a 3-dimensional biomechanical model at such articulations as wrist, elbow, shoulder, the third lumbar spine, hip, knee, and ankle joints. The linear correlation and the two way analysis of variance were applied to the calculated joint moments in order to investigate inter-subject and inter-trial varations. The results obtained showed that the largest magnitude and deviation of moment was found at the third lumbar spine, that any linear relationship could not be found between moment and its equivalents attempted in this study, and that the maximum value and deviation of moment acting on each joint were statistically the same for all three trials but those were statistically not the same for all six subjects.

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Measurement of the Compressive Force on the Knee Joint Model fabricated by 3D Printing (3D 프린팅으로 제작된 무릎 관절모델의 압축력 측정)

  • Jeong, Hoon Jin;Jee, Min-Hee;Kim, So-Youn;Lee, Seung-Jae
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.13 no.2
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    • pp.1-7
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    • 2014
  • Recent experimental observations support the hypothesis that mechanical stimuli play a role in regulating the specialized molecular expression of articular cartilage in vitro and in vivo. Other studies have demonstrated that the continuous passive motion(CPM)bioreactor for whole joints can provide a platform for possible future in vitro studies and applications, including possible interactions of bio-mechanical and biochemical signals. In this study, we have developed acustom-made bioreactor capable of bending and stretching with circular type motion, and a biomimetic knee joint model, using a 3D printer. This system could be used to investigate the effects of rehabilitative joint motion of dynamic culture.

Estimation of Muscle-tendon Model Parameters Based on a Numeric Optimization (최적화기법에 의한 근육-건 모델 파라미터들의 추정)

  • Nam, Yoon-Su
    • Journal of the Korean Society for Precision Engineering
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    • v.26 no.6
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    • pp.122-130
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    • 2009
  • The analysis of human movement requires the knowledge of the Hill type muscle parameters, the muscle-tendon and moment arm length change as a function of joint angles. However, values of a subject's muscle parameters are very difficult to identify. It turns out from a sensitivity analysis that the tendon slack length and maximum muscle force are the two critical parameters among the Hill-type muscle model. Therefore, it could be claimed that the variation of the tendon slack length and maximum muscle force from the Delp's reference data will change the muscle characteristics of a subject remarkably. A numeric optimization method to search these tendon parameters specific to a subject is proposed, and the accuracy of the developed algorithm is evaluated through a numerical simulation.

Development and Evaluation of a Joint Health Self-management Program for the Elderly with Knee Osteoarthritis in Communities: Applying the IMB Model (재가 무릎 골관절염 환자의 관절건강 자가관리 프로그램 개발 및 효과: IMB 모델 적용)

  • Kim, Young-il;Park, Jeong Sook
    • Research in Community and Public Health Nursing
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    • v.28 no.1
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    • pp.55-68
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    • 2017
  • Purpose: The purpose of this study is to develop and evaluate a joint health self-management program for knee osteoarthritis elders in communities. Methods: This program was developed based on the IMB (information-motivationbehavioral skills) model. The program methods include education, setting and achieving goals, sharing experiences, telephone counseling, and self-monitoring. The topics of the program include joint assessment, exercise, massage, joint protection, medication, depression management, diet, and healthcare approach. The research was conducted following the principles of the nonequivalent control group pretest-posttest design. Participants were 26 subjects in the experimental group, and 27 subjects in the control group. The experimental group participated in the self-management program, and the control group received general education. Results: Both post-test scores of personal motivation, social motivation, behavior skill, self-management behavior, joint pain, joint stiffness, physical function disability, right knee extension, left knee extension, and depression were significantly different between the experimental group and the control group. Conclusion: The results of the study can be used to develop and standardize a systematic joint health self-management program. Further research is highly recommended to develop a strategy to continuously facilitate self-management of osteoarthritis patients' procedures.

The effect of 3 weeks high altitude skiing training on isokinetic muscle function of cross-country skierst (3주간의 고지대 스키훈련이 크로스컨트리 스키 선수의 등속성 근기능에 미치는 영향)

  • Choi, Yong Chul
    • Journal of the Korea Convergence Society
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    • v.9 no.11
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    • pp.465-477
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    • 2018
  • The purpose of this study is to analyze the effect of three - week high altitude ski training on the myocardial performance of cross - country skiers and to provide basic data for the future improvement of cross - country skiers'. The subjects were 6 cross - country skiing male college athletes. To investigate the effects of periodic and high altitude training on cross - country skiers, a general linear model ANOVA with repeated measure And analyzed using the Paired Samples t-test. In high altitude ski training for 3 weeks, the body composition did not change but the isokinetic muscular function of the shoulder joint, hip joint, knee joint, and ankle joint was decreased. Therefore, further study is needed if it is considered that continuous strength training should be performed during the ski training period such as SP period.

Regulatory mechanism of Angelica Gigas extract powder on matrix metalloproteinases in vitro and in vivo model (참당귀 추출분말이 in vitro and in vivo model에서 MMPs 조절 기전)

  • Kwon, Jin-Hwan;Han, Min-Seok;Lee, Yong-Moon
    • Analytical Science and Technology
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    • v.28 no.6
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    • pp.361-369
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    • 2015
  • The precise mechanism underlying the therapeutic efficacy of an extraction powder of Angelica gigas (AGE) for the treatment of degenerative osteoarthritis was investigated in primary cultured rabbit chondrocytes and in a monosodium-iodoacetate (MIA)-induced osteoarthritis rat model. The treatment with AGE (50 μg/mL) effectively inhibited NF-B activation. The anti-inflammatory mechanism was clarified by gelatin zymography and western blotting measurements of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activities. The AGE (50 μg/mL) treatment significantly reduced MMP-9 activity. The constituents of AGE— decursinol, decursin, and decursinol angelate—were determined by LC-MS/MS after a 24 hr treatment of rabbit chondrocytes. The contents of the major products, decursin and decursinol angelate, were 3.62±0.47 and 2.14 ±0.36 μg/mg protein, respectively in AGE-treated (50 μg/mL) rabbit chondrocytes. An in vivo animal study on rats fed a diet containing 25, 50, and 100 mg/kg AGE for 3 weeks revealed a significant inhibition of the MMPs in the MIA-induced rat articular cartilage. The genetic expression of arthritic factors in the articular cartilage was examined by RT-PCR of collagen Type I, collagen Type II, aggrecan, and MMP (MMP3, MMP-9, MMP13). Specifically, AGE up-regulated the expression of collagen Type I, collagen Type II, and aggrecan and inhibited MMP levels at all tested concentrations. Collectively, AGE showed a strong specific site of action on MMP regulation and protected against the degeneration of articular cartilage via cellular regulation of MMP expression both in vitro and in vivo.

Influence of Diode Laser (808 nm) on a Rat Anterior Cruciate Ligament Transection Model of Osteoarthritis (전십자인대 단열을 통한 랫드 골관절염모델에서 다이오드레이져 (808 nm)의 영향)

  • Park, Seongkyu;Minar, Maruf;Hwang, Yawon;Kim, Somin;Park, Minhyeok;Choi, Seok-Hwa;Kim, Gonhyung
    • Journal of Veterinary Clinics
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    • v.30 no.5
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    • pp.346-352
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    • 2013
  • The study was aimed to investigate the influence of diode laser on osteoarthritis (OA) of stifle joint induced by anterior cruciate ligament transection (ACLT). Sixty 10-week-old male Sprague-Dawley rats were used in this study. Right stifle joint was operated to create ACLT or sham. There were five study groups: control, Sham, ACLT, ACLT + Laser irradiation (ACLT+L) and ACLT + meloxicam administration (ACLT+M). Low-level laser therapy (LLLT) was applied at the operated stifle joint twice a week using an 808-nm indium-gallium-arsenide (InGaAs) diode laser during 8-week experimental period. Radiographical, gross morphological and histopathological findings were examined at 2, 4 and 8 weeks post-surgery. Radiography, CBC and chemistry tests showed no significant difference between groups. ACLT+L group showed remarkable cartilage damages compared with sham group morphologically and histopathologically at 2, 4 and 8 weeks after surgery. ACLT+M group also had more cartilage damages compared with sham group. Low-level laser therapy (LLLT) showed limitation to prevent progression of OA in the rat anterior cruciate ligament transection models; on the contrary it accelerated cartilage damage. It is assumed that the aggravating results of LLLT in this study might be due to excessive unstable movement of stifle joint from the pain-relieving effect of LLLT, rather than direct damaging effect of irradiation since LLLT did not affect cell viability.

The Effect of Cultured Perichondrial Cell Sheet Covered Highly Active Engineered Cartilage: in vivo Comparative Assessment (배양연골막이 피복된 고효능 인공연골의 생체내 효과)

  • Park, Se-Il;Moon, Young-Mi;Jeong, Jae-Ho;Jang, Kwang-Ho;Ahn, Myun-Hwan
    • Journal of Veterinary Clinics
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    • v.28 no.5
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    • pp.486-496
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    • 2011
  • A special mesenchymal tissue layer called perichondrium has a chondrogenic capacity and is a candidate tissue for engineering of cartilage. To overcome limited potential for chondrocyte proliferation and re-absorption, we studied a method of cartilage tissue engineering comprising chondrocyte-hydrogel pluronic complex (CPC) and cultured perichondrial cell sheet (cPCs) which entirely cover CPC. For effective cartilage regeneration, cell-sheet engineering technique of high-density culture was used for fabrication of cPCs. Hydrogel pluronic as a biomimetic cell carrier used for stable and maintains the chondrocytes. The human cPCs was cultured as a single layer and entirely covered CPC. The tissue engineered constructs were implanted into the dorsal subcutaneous tissue pocket on nude mice (n = 6). CPC without cPCs were used as a controls (N = 6). Engineered cartilage specimens were harvested at 12 weeks after implantation and evaluated with gross morphology and histological examination. Biological analysis was also performed for glycosaminoglycan (GAG) and type II collagen. Indeed, we performed additional in vivo studies of cartilage regeneration using canine large fullthickness chondrial defect model. The dogs were allocated to the experimental groups as treated chondrocyte sheets with perichondrial cell sheet group (n = 4), and chondrocyte sheets only group (n = 4). The histological and biochemical studies performed 12 weeks later as same manners as nude mouse but additional immunofluorescence study. Grossly, the size of cartilage specimen of cPCs covered group was larger than that of the control. On histological examination, the specimen of cPCs covered group showed typical characteristics of cartilage tissue. The contents of GAG and type II collagen were higher in cPCs covered group than that of the control. These studies demonstrated the potential of such CPC/cPCs constructs to support chondrogenesis in vivo. In conclusion, the method of cartilage tissue engineering using cPCs supposed to be an effective method with higher cartilage tissue gain. We suggest a new method of cartilage tissue engineering using cultured perichondrial cell sheet as a promising strategy for cartilage tissue reconstruction.