• Journal of Digital Convergence
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• v.12 no.4
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• pp.419-425
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• 2014

The objective of this study is to estimate the inhibition of skin melanin formation by extract of Angelica koreana and Cnidium monnieri and the possibility of functional cosmetic materials through anti-irritation and stability test. The extract used in this experiment is White-$AK^{TM}$ and the INCI name is Osthole. The main component of White-$AK^{TM}$ was identified as coumarin and EC50 value was 2.7ppm by mouse melanoma B 16 cell test. White-$AK^{TM}$ showed inhibitory effects 100 times lower concentration than arbutin. The main mechanism for skin whitening effect thought to be inhibition of tyrosinase-related gene expression. The basic essence formulation of White-$AK^{TM}$ 5% solution applied to the skin showed the effect of relieving skin irritation. White-$AK^{TM}$ in an opaque container, under UV conditions for 4 weeks, and showed close to 100% recovery and 97% recovery under $50^{\circ}C$ for 4 weeks. Therefore, it is thought that White-$AK^{TM}$ which helps skin whitening, relieving skin irritation and stable in UV condition is able to be used as the functional component in the cosmetic formulation.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.10a
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• pp.187-188
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• 2000

최근 자외선 차단제의 사용이 증가하면서 자외선 차단제 도포로 인한 자극성 피부염, 접촉 피부염, 광알레르기성 또는 광독성 피부염 등의 보고가 증가하고 있어 피부에 자극이나 알레르기 반응, 독성을 나타내지 않는 적절한 천연소재 자외선 차단제의 개발에 대한 필요성이 증대되고 있다. 이에 본 연구에서는 새로운 자외선 차단제 개발을 위한 기초 자료를 확보하기 위하여, 국내산 해조류 중의 자외선 흡수 물질의 분포를 조사하였다. (중략)

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.4
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• pp.341-352
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• 2021

Acerola is an excellent ingredient because of its high natural vitamin C content, but it is difficult to stabilize and has hardly been studied as a cosmetic material. Therefore, this study developed a mixed liposome preparation for stabilizing acerola extract. As a safety test, the skin irritation test was evaluated by BCOP assay and HET-CAM assay. We evaluated the inhibition of tyrosinase activity, the whitening effect of melanin production, and the wrinkle effect of prochloragentype-I C-peptide production, and confirmed the possibility of functional cosmetics. In addition, a cream of liposomes containing acerola extract mixture was developed to evaluate the clinical studies of skin wrinkles and whitening. BCOP assay, HET-CAM assay and human skin primary irritation test results of liposomes containing acerola extract mixture showed no irritation and were safe from skin and eye. The result of tyrosinase activity by 75.8% at 1,000 ㎍/mL. As a result of the melanogenesis inhibition test, liposome with acerola extract showed the melanin content by 46.2% at 1,000 ㎍/mL that does not effect the viability of the B16F10 cell line. The result of collagen production test using ELISA kit, liposomes containing acerola extract mixture showed collagen synthesis ability by 152.1% at 1,000 ㎍/mL that does not affect the viability of the HS68 cell line. But it did not showed any inhibition of collagenase (MMP-1) activity at all concentrations in the MMP-1 activity inhibition test in the HS68 cell line. We performed clinical studies for the whitening and skin-wrinkle activity of cream containing acerola extract mixes liposome, was showed that the melanin contents and wrinkle was statistically significant reduction. These results suggest that liposomes containing acerola extract mixture have safe natural material, and skin wrinkle, whitening effects allowing their application in cosmetics as a natural product.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.21 no.1
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• pp.67-83
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• 1995

Skin irritations accompany the series of complex, physical and chemical changes. Alternative methods which reflect the biological phenomenon more sensitively are necessary for the purpose of reducing the animal tests and improving the accuracy and reliability. Chorioallantoic membrane (CAM) vascular assay is a in vitro method which utilizes the chorioallantoic membrane of hen's fertilized egg of about 10 days old. Test substances are placed directly onto the surface of the CAM and 1 hour later the CAM vasculature is subjectively evaluated to determine with a chemically related-injurious reponse including the appearance of haemorrage, congestion, coagulation, and so on. In this research, using the various surfactants, the correlations of CAMVA with in vivo models (intradermal safety test and human primary irritation test) were investigated. And CAMVA closely correlated to intradermal safety test (r=0.89) and human primary irritation test (r=0.90). From the result, it seems that CAMVA can also be used as a method for predicting the skin irritaions.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.10a
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• pp.386-388
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• 2018

본 논문은 사회적 상호작용 결여로 감정 기복이 심하고 스트레스로 인해 정서불안 증세를 보이는 자폐 범주성 장애아동의 감정 상태를 인식하기 위한 목적으로 4가지 감정 자극에 대하여 생체신호를 분석하고 K-Means 알고리즘을 적용하여 획득한 정보로부터 감정 상태를 인식하는 방법을 제안한다. 실험구성은 참가자가 주어지는 감정자극 영상을 시청하는 동안 맥파 및 피부전도 센서를 이용하여 생체신호를 측정한 후 자율신경 비율을 나타내는 LF/HF의 심박 정보와 피부 반응 정보를 정량적으로 분석하였고, 추출된 정보로부터 K-Means 알고리즘을 적용하여 감정 상태를 분류하는 과정으로 진행된다. 총 3명의 일반인을 대상으로 실험을 진행하였으며, 4가지 감정 자극에 대한 실험을 수행한 결과, 생체신호 측정을 이용한 감정인식 방법이 제시되는 감정 자극을 충분히 분류할 수 있음을 확인할 수 있었다.

• Korean Chemical Engineering Research
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• v.46 no.2
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• pp.227-232
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• 2008

In this research, organic extracts from roots of platycodon grandiflorum was tested to see the possibility for cosmetic natural surfactant. Interfacial properties of extracts of Platycodon grandiflorum was checked for interfacial tension, forming force, dispersion force, emulsion force, emulsion activity, and emulsion stability. At 0.005 wt% concentration, the interfacial tension against the caster oil was 11.5 dyn/cm which was lower than that of Tween 40 and quillaja bark. Extracts of Platycodon grandiflorum showed excellent emulsification activity and stability for cosmetic oils such as olive oil, soybean oil, and canola oil. In patch test using 2-5% of the extract and glycerin, the extract showed mild skin irritation. From the experiment, the extracts of platycodon grandiflorum root showed good interfacial properties as a cosmetic agent with minor skin irritation.

• Journal of the Institute of Convergence Signal Processing
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• v.22 no.1
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• pp.14-19
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• 2021

This study has attempted to use ozone for the treatment of skin diseases as research results that ozonated olive oil has an excellent therapeutic effect on skin diseases are known. However, there is hardly any accurate data in Korea. Usually, animal tests related to cosmetics are not performed, but toxicity tests were conducted because they were absolutely necessary. In general, there are not many cases of measuring actual data through animal tests for the purpose of confirming the performance of cosmetics, but in the case of toxicity tests, it is recommended to accurately measure skin reactions, so this experiment was conducted. In this experiment, in order to evaluate the skin irritation of ozonated oil (high concentration) on the rabbit, the test substance was applied to the back of the rabbit for 24 hours, and then mortality, general symptoms and skin irritation were evaluated. Experimental Results As a result of evaluating the treatment site of the test substance after a certain period of time, no skin irritation was observed in all animals.

• 월간산업보건
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• s.292
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• pp.11-15
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• 2012

페놀의 작업 노출에 대한 TLV-TWA는 5ppm ($19mg/m^3$)으로 눈과 호흡기계의 자극과 함께 심장, 간, 신경 독성 영향을 최소화하기 위해 설정되었다. 페놀은 증기상과 액상 또는 고체상 형태로 피부에 접촉되면 흡수되어 심각한 계통 영향을 유발하여 최종적으로 사망에 이르게 한다. 피부를 통한 흡수율은 흡입에 의한 체내 흡수율과 거의 동일한 수준이다. 따라서 현재 Skin notation(피부흡수 경고) 물질로 지정되어 있다. 발암성 여부를 결정하기 위한 동물 실험 결과에서는 음성 또는 발암성을 판정하기에는 데이터가 부족한 관계로 A4로 지정되어 있다. 또한 SEN과 TLV-STEL도 유용한 자료의 부족으로 아직 설정되지 않고 있다. 페놀은 BEIs로 활용될 수 있는 기질 중 하나이다.

• 월간산업보건
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• s.300
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• pp.4-7
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• 2013

m-Phenylenediamine의 직업적 노출기준 TLV-TWA $0.1mg/m^3$은 간 손상을 최소화하기 위하여 권고하였다. 또한 이 수준은 피부 자극이나 피부염을 예방하기 위한 기준이다. 흰쥐와 생쥐에게 m-phenylenediamine을 처치한 후의 발암성이 음성적이거나 명확하지 않은 결과로 A4(Not Classifiable as a Human Carcinogen, 사람에게 발암성으로 분류되지 않음)로 권고하였다 m-Phenylenediamine을 생산하는 근로자들에서 피부 알레르기반응이 있었다는 제한적인 자료도 있었지만 피부(Skin)와 감작제(SEN)의 경고주석 그리고 TLV-STEL을 권고하기에는 유용한 자료가 충분하지는 않다.

• 월간산업보건
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• s.371
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• pp.28-31
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• 2019

피부, 눈 및 상부 호흡기의 자극 가능성을 최소화하기 위해, 인산(Phosphoric acid)에 대한 직업적 노출기준 TLV-TWA를 $1mg/m^3$, 그리고 TLV-STEL을 $3mg/m^3$으로 권고하였다. 수분이 있는 상태에서 인산 먼지나 인산 케이크에 의한 피부 접촉은 특히 자극적이다. TLV-TWA의 권고수준은 산에 노출 경험이 없는 노동자들에게 인후염을 일으키는 인산의 공기 중 농도보다 낮은 수준이다. 또한 인산 노출에 순응된 노동자들에게 유발할 수 있는 농도보다는 훨씬 더 낮은 수준이다. 피부(Skin), 감작성(SEN) 또는 발암성 경고표지(Notation)를 권고하기에는 충분한 자료가 없다.