• The Korean Journal of Zoology
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• v.32 no.4
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• pp.412-419
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• 1989

Monoclonal antibodies (MAbs) reacting with the plasmalemma of Amoeba proteus were produced. Specificity of the 3 MAbs was determined by transfer blotting of the SDS polvacryfamide gel. AMS antibody reacted with the mucopolysaccharide bands of the spacer gel, 220 KD and 50 KD proteins of the resolving gel. The maior glycoprotein bands (175 KD, 165 KD) and 50 KD protein of the plasmalemma were recognized by AUG antibody. A third, AMP antibody reacted with the 50 KD protein only. In immunofluorescence microscopy of the enzyme treated cells, the antigens of these MAbs were sensitive to proteases, but not sensitive to neuraminidase. In the assay of cell to substratum attachment after binding with the antibody, AMG and AMP antibodies exerted no effect, but AMS hindered the attachment and cell spreading. Thus the effective components of the plasmalemma in cell to substratum attachment appear to be the mucopolysaccharides and 220 KD protein. The membranes of latex particle infested phagosomes did not show any distinction from the plasmalemma in fluorescence microscopy. Phagosome membranes of amoebae appear to be derived from the plasma membrane without selection in terms of the antigen composition. Amoeba Proteus의 세포막과 반응하는 단세포군 항체를 생산하였다. SDS polyacrylamide gel을 transfer blotting하여 이들 항체의 반응 특이성을 조사해 본 결과 AMS 단항체는 PAS로 염색되는 spacer gel의 mucopolysaccharide 린드, resolving gel의 220 KD 및 50 KD 단백질과 반응하였으며, 세포막의 주요 당단백질인 175 KD 및 165 KD 빈드와 50 KD 단백질은 AMG 단항체에 의해서 인지되었다. 그리고 AMP단항체는 공통인 50 KD 단백질과 특이하게 반응하였다. 효소처리한 아메바의 면역형광칠미경적 조사에서 이들 항체에 대한 항원분자들은 모두 단백질분해효소에 민감하였으며 neuraminidase에 대해서는 변화가 없었다. 이들 항체를 결합시킨 아메바의 용기표면 부착 가능성을 분석한 결과 AMP 및 AMG 단항체는 아무런 영향을 미치지 못하였으며 AMS 단항체는 세포의 용기표면 부착 및 세포의 펴짐을 저해하였다. 따라서 아메바의 용기표면 부착은 mucopolysaccharide 및 220 KD 단백질에 의해서 매게되는 것으로 나타났다. 그리고 latex particle을 담고 있는 식포막은 면역형광형미경적 조사에서 세포막과 차이가 없었다. 따라서 겐포막은 항원 조성에 있어서 비 선택적으로 세포막에서 유도되는 것으로 나타났다.

• The Korean Journal of Zoology
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• v.37 no.1
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• pp.19-32
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• 1994

가장 효과적인 H-2b 항원에 대한 시험관내 항체 생산 조건을 찾기 위하여 근교계 생쥐인 C57BL/6BySnj의 비장세포를 UV로 불활성학 시킨 후 항원으로 사용하고, A/wySnJ$\times$Sm/J(ASmJF1, hybrid)의 비장세포를 항원 수용자 계통으로 하여 5-7일동안 배양기에서 항체 생산을 유도하였다. 본 실험에서 T 임파구 대식세포와 임파구 분화 촉진 인자인Concanavalin A Lipopolvsaccharide. Pokeweed mitogen 등을 사용하여 20가지 조건으로 실험을 수행하여, 항체 생성 여부는 보체 의존성 세포 장애 실험과 면역 효소법에 의해 조사하였다 그 결과 모든 조건에서 항체생산이 확인되었으며. 가장 좋은 시험관내 항체 생산 조건으로는 T 임파구와 대식세포를 함께 사용하여 면역시킨 것이 가장 효과적이었다. 이 방법을 이용하여 항체 생산을 유도한 후 5일째 면역된 비장세포를 Sp2/0-Ag 14와 세포 융합시켜 H-2b 마우스의 체포 표면 항원에 대한 단일군항체 생산을 시도하였다. 또한 생체내 면역 방법과 비교하기 위해 6주간 C57BL/6BySnJ의 비장세포를 복강내에 주사하여 같은 조건으로 세포융합을 시도하였다. 그 결과 H-2b 세포의 표면 항원에 대한 항체 생산을 하는 세포군은 시험관내 면역 방법에서 3개 생체내 면역 방법에서 4개부 확인되었다.

• Journal of agricultural medicine and community health
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• v.31 no.3
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• pp.245-253
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• 2006

Objectives: Since 1983, vaccination for Hepatitis B virus has been recommended for all neonates in Korea. The objectives of this study was to assess the changing trend of the positive rate of HBsAg and anti-HBs in university students in one province. Methods: The study population consisted of one university students who taken physical exam in 2004, which included 8,527 students (3,391 males and 5,136 females). Serum HBsAg and anti-HBs were detected by immunochromatography method. Results: The positivities of HBsAg and anti-HBs were 2.7% and 61.1%. The positivity of HBsAg was significantly higher in males(p< .05), while that of anti-HBs was significantly higher in females(p< .05). There was some differences of HBsAg positivities among age groups. But there was no correlation between age and the positivities. There was some differences of anti-HBs positivities among age groups. But there was no correlation between age and the positivities. Conclusion: The positivity of HBsAg has significantly decreased and that of anti-HBs has increased with years. It was suggested that the changes of positivity were caused by nationwide hepatitis B vaccination program.

• Journal of Sensor Science and Technology
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• v.7 no.4
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• pp.263-270
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• 1998

Surface Plasmon Resonance (SPR) sensor system, has rapid response and high sensitivity, can be applicable for detecting reaction times of many biospecific interactions. A SPR sensor system was constructed to detect the antigen-antibody reactions of salmonella and hIgG (human immunoglobulin G). Sensor chips made of gold thin film were used for detecting biological bindings of antigen and antibody reactions. The antigen and antibody reactions for salmonella and hIgG were carried out with various time intervals to observed characteristics of these reactions using SPR sensor system. The resonance angle shift changes were clearly observed at the time of salmonella or hIgG antibody injection into sample cell since each antibody was self-assembled on gold chip surface of the sensor. It was found that the antibodies of salmonella and hIgG reacted with its sensor chip surface in 10 minutes and 60 minutes respectively. And the antigens of both salmonella and hIgG were bound to its antibody within 1 minute.

• Microbiology and Biotechnology Letters
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• v.17 no.5
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• pp.494-498
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• 1989

Hybridoma cell lines, which secrete monoclonal antibodies (mAbs) against the surface antigens of Cryptosporidium parvum Sporozoites, were produced by fusing spleen cells of C. parvum Sporozoite-immunized mice with P3-X63-Ag8 myeloma cells. Two cloned antibody-secreting cell lines, Kor1 and Ea2, were established and produced IgG1 and IgG2a antibodies, respectively. Percoll-purified sporozoites were solubilized and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Western blot assay demonstrates that an antigen of 20-kDa was bound by monoclonals. By indirect immunofluorescence microscopy, mAb exhibited uniform binding to the sporozoite surface.

• Journal of the Korea Convergence Society
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• v.11 no.1
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• pp.77-82
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• 2020

In order to analyze the positive rate of hepatitis B surface antibody by age before and after hepatitis B vaccination, 13,855 serum specimens who were referred for the hepatitis B surface antibody test at the General Hospital of Jeju Hospital were examined by CMIA method. The positive rate of HBs Ab was 5,176 (37.36%). The positive rate according to gender was 40.13% for female and 34.77% for male. The age group with the highest HBs Ab average was in their 40's (399.86 mIU/mL), while the lowest age group was in the 90's (211.50 mIU/mL). It is remarkable that the age group with the highest HBs Ab positive rate is in the 30's, and that teenagers (age group of 10-20 years) had the lowest positive rate. Especially 15, 18, 19 years old was statistically significant. Consideration should be given to determining the titer of vaccination and to clarify the timing of vaccination.

• Environmental Mutagens and Carcinogens
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• v.11 no.2
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• pp.134-140
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• 1991

B형 간염바이러스의 표면항원, 파상풍, 디프테리아균의 toxoid, 백일해의 혼합백신인 PDT-Hepa의 효능을 실험하여 다음과 같은 결과를 얻었다. 시험물질을 기니픽에 접종하였을 때 B형 간염 바이러스의 표면 항원과 파상풍 및 디프테리아균의 toxoid에 대한 항체형성이 뚜렷이 관찰되었으며, 항체형성의 정도는 접종용량에 비례하였다. 백일해의 경우는 시험물질을 마우스에 접종시킨 후 마우스에 감수성이 있는 Bordetella pertussis 18323주로 직접 공격 접종하여 이 균에 대한 방어력이 형성되어 있음을 관찰하였으며, 이러한 방어정도는 투여 용량에 비레함을 알 수 있었다.

• The Korean Journal of Zoology
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• v.39 no.1
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• pp.12-20
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• 1996

A multipotential hematopojetic cell line, 1(562 cell, was differentiated into megakaryocyte by a chemical inducer, PMA, with an enhanced expression of gpIlla accompaning with a distinct morphological change. On the other hand, 1(562 cells were differentiated into erythrocytes by other chemical inducers, DMSO or butyrate, with a concomitant increase in hemoglobin accumulation. An antigen of apparent molecular weight of 61 kDa was identified on the surface of 1(562 cells by using monoclonal antibody raised against 1(562 cells. The antigen was considered to be a glycoprotein molecule rich in sialic acids and the epitope of antigen was sensitive to neuraminidase digestion or peroxidase oxidation, but resistant to heat treatment. The 61 kDa surface antigen was increased or decreased in its expression along differentiation of 1(562 cells into megakaryocytes or erythrocytes, respedively.

• The Korean Journal of Mycology
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• v.17 no.2
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• pp.82-90
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• 1989

Detection of antibody against pathogenic fungi in serum specimens of the patients with pulmonary tuberculosis or other lung diseases has been carried out(male) using the indirect fluorescence antibody technique and immunodiffusion tests. Immunodiffusion tests revealed that 104(36.5%) out of 285 patients examined showed a positive precipitin reaction against one or more of fungal antigens. The majority of ID positive patients 64(61.5%) reacted with Aspergillus fumigatus antigen and 49(47.1%) patients reacted with Candida albicans antigen ID positive reaction to A. fumigatus was found little more frequently among male patients, while Candida albicans reactors were found more frequently among female patients. Age distribution of ID positive reactors was high(49.1-43.3%) in age group of 40-59 years, but least or none in age group of less than 30 years. Age of fungal mycelium used as antigen did not effect sensitivity of the indirect flubrescence (IF) technique in detecting antibody to A. fumigatus. Antibody class against A. fumigatus that showed highest titer was IgG and thus FITC labeled anti-IgG immunoglobulin shoul be preferable. As relatively large amount of cell wall components of Aspergilli shared antigenically, a considerable cross-reaction was observed among A. fumigatus, A. flavus and A. niger, but not much with C. albicans. While (IF) has much better sensitivity when compared with ID, relative specificity of the latter procedure cannot to be overried, so that they could be batter used together in order to obtain quantitative measurement of antibody with relative specificity.

• 대한예방의학회:학술대회논문집
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• 1996.10a
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• pp.377-378
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• 1996