• Korean Journal of Pharmacognosy
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• v.46 no.2
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• pp.123-132
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• 2015

In this study, we carried out quantification analysis of the six marker components, geniposidic acid, chlorogenic acid, geniposide, pinoresinol diglucoside, liriodendrin, and genipin in the 70% ethanol extracts of non-processed Eucommiae Cortex and processed Eucommiae Cortex using a high-performance liquid chromatography coupled with photodiode array detector. The six components were separated on Gemini C₁₈ column (5 μm, 4.6×250 mm) by the gradient elution with 1.0% (v/v) acetic acid in water and 1.0% (v/v) acetic acid in acetonitrile as mobile phase. The flow rate was 1.0 mL/min and the injection volume was 10 mL. The amount of geniposidic acid, chlorogenic acid, geniposide, pinoresinol diglucoside, liriodendrin, and genipin in non-processed Eucommiae Cortex were 1.31, 0.31, 0.66, 0.46, 0.46, and 0.03%, respectively, while the amount of the six compounds in non-processed Eucommiae Cortex were 0.04-0.78, 0.01-0.14%, 0.05-0.63%, 0.01-0.37%, 0.15-0.42%, and not detected, respectively. After processing treatment, the contents of three iridoids, two lingnan, and one phenylpropanoid decreased in Eucommiae Cortex.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.3
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• pp.846-853
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• 2004

This study was carried out for establishment of toxicological monitoring system in oriental medicinal plants. Hence on our research, we used Banha(Pinellia ternata) and Kangbanha, Bubbanha, Jaebanha, Geokbanha by distinguished processing methods. These are frequently used in Bangyakhabpeon, which is one of the most well-known clinical book in oriental medicine. As we reviewed the reported documents, we judge homogentisic acid(HA) and 3,4-dihydroxybenzaldehyde(3,4-DBA) as poisonous substance and to verify its existence, we established analysis condition of HPLC by gaining sequential fraction extracts and studied the degree of its virulence to provide basic information on the guidelines of using this medicine. Optimum condition of HPLC was H₂O : MeOH : CH₃COOH (57:35:8) in HA and 3,4-DBA analysis. HA content of raw Banha was 11.03mg/100g and HA contents of its processed product were decreased. Exceptionally, Jaebanha was increased in 175.97% than raw Banha. 3,4-DBA content of raw Banha was 2.93mg/100g and 3,4-DBA contents of its processed product were decreased. These results will be applies in intake guideline establishment, quality control and stability evaluation of oriental medicinal plants.

• The Korea Journal of Herbology
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• v.22 no.2
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• pp.189-199
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• 2007

Objective : This study was observed the variation of components from Scutellariae Radix (SR) by processing and storage. Methods : The main component of SR is analysed after various processing and also storage condition by HPLC. Result : 1. In wine-fried SR, the contents of baicalein, wogonin and oroxylin-A was significantly increased. However, the content of baicalin was decreased slightly. In charred SR, the contents of baicalein, wogonin and oroxylin- A was significantly increased. However, the content of baicalin was decreased greatly. 2. The contents variation of SR was not almost changed in room and cold condition. But in accelerated condition, the content of baicalin was slightly decreased, and the contents of baicalein, wogonin and oroxylin-A was significantly increased. 3. The contents variation of wine-fried SR was not almost changed in room and cold condition. But in accelerated condition, the content of baicalin was signifjcantly decreased. 4. The contents variation of charred SR was not almost changed in all conditions. Conclusion : The bioactivity of SR and wine fried SR might be changed by the contents varition during longterm storage. charred SR can be preserved more than two years in room and cold condition.

• Journal of the Korean Applied Science and Technology
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• v.16 no.2
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• pp.179-185
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• 1999

The effect of blowing agents and inner temperature on the machanical properties of the flexible polyurethane foams were investigated. In the study used that chemical blowing agents is $H_2O$ and support blowing agents. CFC-11, HCFC-114b, dichloromethane, n-penthane, iso-pentane, cyclopentane. The flexible polyurethane foams were foamed by the density of $0.015{\pm}0.002g/cm^3$ and $0.024{\pm}0.002g/cm^3$ which were used in mechanical properties measurements. Inner temperature was measure as long as the preparation of the flexible polyurethane foams of each blowing agents. The density, tensile strength, elongation, tear strength, compression strength and compression set were measured after 48 hours hardening. The result of the study was optimized dichloromethane and cyclopentane at the support blowing agents.

• Magazine of the Korea Concrete Institute
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• v.9 no.1
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• pp.173-181
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• 1997

The objective of this study is to obtain the mechanical characteristics of prefoarmed lightweight foamed concrete using the polymer ham agent which has high lightness. flowability and strength. For this purpose, the prefoarmed lightweight foamed concrete which was developed to have flow value over 180mm. unit weight between 0.38t/$m^3$ and 0.64t/$m^3$, and compressive strength about 30kg/$cm^2$ was used. This paper presents extensive test data on Young's modulus. Poisson's ratio, stress-strain curve, the characteristics of strength of the foamed concrete and also presents the mechanical characteristics of the foamed concrete with different foam sizes. It is expected that this study provides an importance guide to design and manufacture lightweight foam concrete, so that it helps to expand its structural use.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.4
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• pp.421-432
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• 2016

In order to find new functional materials for the cosmetics application, we investigated anti-inflammatory and whitening effects of the Protaetia brevitarsis seulensis (P. brevitarsis) extracts, which were prepared by the various oriental conversion methods, as follows; fresh, roasted one time, roasted two times, roasted three times, and steamed. 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of the various solvent extracts (80% ethanol, 50% ethanol, ethyl acetate, hexane) of P. brevitarsis extracts were 85.5, 22.4, 37.0 and 19.4% respectively. The 80% ethanol extract with the highest antioxidant activity was used for all experiments. In case of antioxidant activity test of the extracts, all the extracts showed the activities in concentration dependent manner regardless of the sample preparation methods. Superoxide dismutase-like (SOD-like) activities of the extracts roasted three times and steamed were 62.9 and 55.9%, respectively in $500{\mu}g/mL$. Effects of extracts on the inflammation of RAW 264.7 cell induced by lipopolysaccharide (LPS) showed decreasing tendency of $NO{\cdot}$ and prostaglandin $E_2$ ($PGE_2$) production; PBS fresh (38.0%), PBS roasted one time (41.0%), PBS roasted two times (69.8%), PBS roasted three times (70.1%), PBS steamed (78.5%). Intracellular tyrosinase and melanin biosynthesis inhibitory activities of the extracts were decreased in a concentration dependent manner. However, the fresh P. brevitarsis extracts without the oriental conversion method showed 90.7% decrease compared to the control group treated with ${\alpha}$-MSH alone at $500{\mu}g/mL$. Taken together, these results suggest the oriental conversion method can be applied in development of cosmetic materials in order to improve anti-inflammatory and whitening effects of the cosmetics products.

• Herbal Formula Science
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• v.25 no.1
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• pp.11-28
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• 2017

Objectives : The contents of aconitine in aconiti radix lateralis preparata, purified hot water extract of Aconiti Radix lateralis preparata, and purified hot water extract of Sambutang-P that contains Aconiti Radix lateralis preparata was analyzed to compare toxicity. Toxicity of Sambutang-P that contains Aconiti Radix lateralis preparata was assessed with a single oral toxicity test on 6-week-old male and female Sprague-Dawley rats. Methods : 1. The contents of aconitine in Aconiti Radix lateralis preparata, purified hot water extract of Aconiti Radix lateralis preparata, and purified hot water extract of Sambutang-P that contains Aconiti Radix lateralis preparata was analyzed using the purity test according to the "Korean Herbal Pharmacopoeia". 2. 2,000mg/kg was injected for the single oral toxicity test of purified hot water extract of Sambutang-P that contains Aconiti Radix lateralis preparata, and the test was done for a test group (injection) and a control group, each with 5 male and 5 female rats. For 14 days after injection, rats were observed for general symptoms and changes in weight. Afterwards, blood biochemical test, autopsy, and histophathological exam of the liver was conducted. Results : 1. The contents of aconitine was 0.0785% for Aconiti Radix lateralis preparata, 0.1510% for purified hot water extract of Aconiti Radix lateralis preparata, and 0.1248% for purified hot water extract of Sambutang-P that contains Aconiti Radix lateralis preparata. 2. There was no death of either male or female rats in both the control group and the test group (injection of 2,000mg/kg). 3. No unusual symptom was observed in both the control group and the test group (injection of 2,000mg/kg). 4. No significant change in weight was observed for both male and female rats in the test group (2,000mg/kg). 5. The histopathological exam of ALT, AST, ALP, GGT and LDH showed no significant changes for both male and female rats in the test group (2,000mg/kg). 6. According to the autopsy results, no visible abnormality of organs or tissues was found in both the control group and the test group (2,000mg/kg). 7. According to the histopathological exam of the liver, the effect of the injected material was not observed for either male or female rats in the test group (2,000mg/kg). Conclusions : The contents of aconitine in Aconiti Radix lateralis preparata was lower for decoction of Sambutang-P with ginseng radix alba than for decoction of only Aconiti Radix lateralis preparata. This suggests that ginseng radix alba can dilute toxicity of Aconiti Radix lateralis preparata. As for a single oral toxicity test of Sambutang-P that contains Aconiti Radix lateralis preparata, no abnormal reaction was observed even when the injection amount far exceeded a toxic dose or a lethal dose. Thus, it is deemed that using Sambutang-P at a clinically prescribed dose would not lead to hepatoxicity.

• The Korea Journal of Herbology
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• v.22 no.4
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• pp.261-270
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• 2007

Objective : The 5-HMF was not index material suitable to do the quality control of Rehmanniae Radix Preparata. In this study, We estimated the changes of oligosaccharide contents in Rehmanniae Radix Preparata using high-performance anion-exchange chromatography with pulsed amperometric detection(HPAEC-PAD). Methods : The analysis of oligosaccharide was conducted by HPAEC-PAD with Carbopac PA1, $250{\times}4mm$, 5um, and Carbopac PA1 guard column. Column temperature was kept at $30^{\circ}C$. Elution was carried out at 1000 ${\mu}l/min$ with 70mM NaOH and the injection volume was $10{\mu}l$. Each component was detected by PAD. Results : Nine constituents were found from merchandising Rehmanniae Radix Preparata(MR), while seven constituents were found in various processed Rehmanniae Radix Preparata. Not all constituents were defined but stachyose and raffinose were found in all cases. And The most common constituents of Rehmanniae radix was stachyose. In the course of processing, most of stachyose and raffinose were decreased. Stachyose was decreased slowly in the course of processing with rice wine(RR), amomi and rice wine(AR), and crataegi and rice wine(CR). However stachyose was decreased rapidly in the course of processing with fresh rehmannia juice(FR). The method with crataegi and rice wine(CR) showed the smallest decrease of stachyose. And processing method with crataegi and rice wine(CR) showed the most abundant amount for stachyose after the nineth processing. Conclusion : The changes of oligosaccharides in the course of processing were a very important direct barometers to do the quality control and set up a standard of Rehmanniae Radix Preparata.

• The Korea Journal of Herbology
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• v.25 no.4
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• pp.95-102
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• 2010

Objectives : Specifications by Processing Methods of Liriope platyphylla (LP) was not established in korea. We carried out method study for manufacture of LP. Above all, LP were purchased and were processed at four kinds. First group had hearts of swelling roots, not cut bodies(LP-A). Second group had not hearts of swelling roots, not cut bodies(LP-B). Third group had hearts of swelling roots, cut bodies(LP-C). Forth group had an aggregate of hearts of swelling roots(LP-D). Methods : We has gained losses on drying, contents of ethanol-soluble extracts, contents of water-soluble extracts and contents of spicatoside A. Results : 1. Losses on drying of all group were less than 18% that is a standard of chinese pharmacopoeia. 2. Contents of ethanol-soluble extracts at LP-C, LP-D were included more than contents of those in the other groups in significance levels. 3. Contents of water-soluble extracts at LP-A, LP-C were included more than contents of those in the other groups in significance levels. 4. Contents of spicatoside A in LP-B were included more than content of spicatoside A in the other groups in significance levels. Resultingly, standards of LP in korea were proposed compatible proposals suggested as below. 1. Losses on drying of LP were less than 15% that is less than that of chinese pharmacopoeia. 2. Form to boil in water must be cutting body with heart of swelling root. 3. Because there is no content of spicatoside A in heart of swelling root, it is not suited to purpose a index component. Conclusions : The subjects of loss on drying and form of LP to boil in water must be contained in korean pharmacopoeia. Moreover, because of much valuable LP in food and medicine, it is urgently required aspect of index component such as spicatoside B, spicatoside C, flavonoid, polysaccharide having medical actions and so on.

• Herbal Formula Science
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• v.25 no.4
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• pp.553-574
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• 2017

The purpose of this study is to find out effects of prescriptions according to the formula, manipulation of Rhei Rhizoma, configuration. The following results were reached through investigations on the prescriptions using Rhei Rhizoma as a main component. Objectives : Analysis of prescriptions According to the formula : Liquid Extract Prescriptions were used widely on three parts to treat fever and damp heat in the interior organs. Powder Prescriptions were taken with hot water, thin porridge, tea etc. to treat damp heat, congestion of phlegm, acute episodes. Liquid Mixed Pill treat congestion of QI, damp heat, phlegm. Honey Mixed Pill treat accumulated fever, distension, acute excessive fever. Paste Pill treat blood stagnation, excessive toxic-fever, epidemic diseases. External Application treat inflammation by injury, swelling due to severe fever by internal damage. Methods : Analysis of prescriptions According to the manipulation of Rhei Rhizoma : Prescriptions including Liquor processed Rhei Rhizoma treat excessive toxic-fever, congestion of phlegm, blocking orifices on the upper side. Steamed Rhei Rhizoma strengthen effects of making evacuate and cooling of heat. Processed Rhei Rhizoma with vinegar strengthen effects of removing blood stagnation by activating blood movement, releasing gathering. Results : Analysis of prescriptions According to the Composition of Rhei Rhizoma : 41% of the total prescriptions were on the area of less than 20%. In case of lower groups show increased frequency of combination with Pharbitidis Semen, Persicae Semen, Scutellariae Radix and manipulation of baking, steaming, roasting. In case of higher groups show increased frequency of treating excess syndrome, critical illness, acute severe illness, and using proccesed Rhei Rhizoma with vinegar. Treatment of damp heat on the liver and gallbladder, disorder of the spleen and stomach is done mostly by prescriptions on the area of less than 30%. Conclusions : Rhei Rhizoma-Coptidis Rhizoma pair treat damp heat, heat toxins in blood, and Constipation caused by excessive heat. Rhei Rhizoma-Glycyrrhizae Radix pair relieve effects of Rhei Rhizoma passing blocked feces, removing the poison, activating blood movement, releasing gathering with the effects of Glycyrrhizae Radix relaxing tension by harmonizing Middle. Rhei Rhizoma-Magnoliae Cortex pair are used to treat damp heat in middle area, excessive heat in the stomach and intestine. Rhei Rhizoma-Pharbitidis Semen pair act on both blood system and QI system treating edema, damp, stagnation, heat toxins, feces. Rhei Rhizoma-Persicae Semen pair treat blood stagnation with fever on blood system.