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Quantitative Determination of the Six Marker Compounds in Eucommiae Cortex by Processing Method  

Seo, Chang-Seob (K-herb Research Group, Korea Institute of Oriental Medicine)
Kim, Jung-Hoon (K-herb Research Group, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (K-herb Research Group, Korea Institute of Oriental Medicine)
Kim, Byoung-Soo (Department of Physiology, College of Korean Medicine, Daejeon University)
Publication Information
Korean Journal of Pharmacognosy / v.46, no.2, 2015 , pp. 123-132 More about this Journal
Abstract
In this study, we carried out quantification analysis of the six marker components, geniposidic acid, chlorogenic acid, geniposide, pinoresinol diglucoside, liriodendrin, and genipin in the 70% ethanol extracts of non-processed Eucommiae Cortex and processed Eucommiae Cortex using a high-performance liquid chromatography coupled with photodiode array detector. The six components were separated on Gemini C18 column (5 μm, 4.6×250 mm) by the gradient elution with 1.0% (v/v) acetic acid in water and 1.0% (v/v) acetic acid in acetonitrile as mobile phase. The flow rate was 1.0 mL/min and the injection volume was 10 mL. The amount of geniposidic acid, chlorogenic acid, geniposide, pinoresinol diglucoside, liriodendrin, and genipin in non-processed Eucommiae Cortex were 1.31, 0.31, 0.66, 0.46, 0.46, and 0.03%, respectively, while the amount of the six compounds in non-processed Eucommiae Cortex were 0.04-0.78, 0.01-0.14%, 0.05-0.63%, 0.01-0.37%, 0.15-0.42%, and not detected, respectively. After processing treatment, the contents of three iridoids, two lingnan, and one phenylpropanoid decreased in Eucommiae Cortex.
Keywords
Eucommiae Cortex; Processing Method; Geniposide; Geniposidic Acid; Pinoresinol Diglucoside;
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