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http://dx.doi.org/10.15230/SCSK.2016.42.4.421

Anti-inflammatory and Whitening Effects of Protaetia brevitarsis Seulensis Extracts by Oriental Conversion Methods  

Sung, Gyeong Ah (Department of Fine Chemistry, Cosmetic R&D center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology)
Kim, Mi Hye (Department of Funeral Service Manegement, Sorabol College)
Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.42, no.4, 2016 , pp. 421-432 More about this Journal
Abstract
In order to find new functional materials for the cosmetics application, we investigated anti-inflammatory and whitening effects of the Protaetia brevitarsis seulensis (P. brevitarsis) extracts, which were prepared by the various oriental conversion methods, as follows; fresh, roasted one time, roasted two times, roasted three times, and steamed. 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of the various solvent extracts (80% ethanol, 50% ethanol, ethyl acetate, hexane) of P. brevitarsis extracts were 85.5, 22.4, 37.0 and 19.4% respectively. The 80% ethanol extract with the highest antioxidant activity was used for all experiments. In case of antioxidant activity test of the extracts, all the extracts showed the activities in concentration dependent manner regardless of the sample preparation methods. Superoxide dismutase-like (SOD-like) activities of the extracts roasted three times and steamed were 62.9 and 55.9%, respectively in $500{\mu}g/mL$. Effects of extracts on the inflammation of RAW 264.7 cell induced by lipopolysaccharide (LPS) showed decreasing tendency of $NO{\cdot}$ and prostaglandin $E_2$ ($PGE_2$) production; PBS fresh (38.0%), PBS roasted one time (41.0%), PBS roasted two times (69.8%), PBS roasted three times (70.1%), PBS steamed (78.5%). Intracellular tyrosinase and melanin biosynthesis inhibitory activities of the extracts were decreased in a concentration dependent manner. However, the fresh P. brevitarsis extracts without the oriental conversion method showed 90.7% decrease compared to the control group treated with ${\alpha}$-MSH alone at $500{\mu}g/mL$. Taken together, these results suggest the oriental conversion method can be applied in development of cosmetic materials in order to improve anti-inflammatory and whitening effects of the cosmetics products.
Keywords
Protaetia brevitarsis seulensis; anti-inflammation; whitening; oriental conversion method; cosmetics;
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