• Journal of Bio-Environment Control
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• v.21 no.2
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• pp.127-133
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• 2012

Inhibition effects on spore germination and mycelia growth for pepper anthracnose fungi (Collectricum acutatum) were investigated in vitro using eco-friendly agricultural materials as well as ecofriendly pesticides. The inhibition effect on mycelia growth of anthracnose fungi is the highest when the anthracnose mycelia were treated with a pesticide (commercial name: Koreayeok) that contains a mixture of Bacillus subtilis and Panibacillus polymyxa, resulting in 100% inhibition of the mycelia growth. Meanwhile, the range of 20~40% inhibition effects on the growth of anthracnose mycelia was observed with other commercial agricultural materials. The significant inhibition effects on spore formation of anthracnose fungus were shown in vitro with two water dispersible pesticides containing sulfur [BTB (100%) and SulfurStar (95.1%)], Koreayeok (95.0%), Borstar (99.0%) containing Bordeaux mixture, and Jihabudea-KM containing Psedomonas spp. (96.1%), respectively. Taken from these in vitro results of inhibiting of the spore germination and mycelia growth together, Koreayeok is the most effective on control of pepper anthracnose disease in vitro. In addition, two water dispersible pesticides containing sulfur (BTB and SulfurStar) and Borstar (99.0%) containing Bordeaux mixture are also significantly applicable to prevent pepper plants from anthracnose disease in vitro. It remains to be determined whether the selected eco-friendly agricultural materials in effective control of anthracnose in vitro can be used to control pepper anthracnose in field.

• Journal of the Korean Institute of Landscape Architecture
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• v.31 no.4
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• pp.90-100
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• 2003

본 연구는 기존 건축물 옥상녹화에 이용 가능한 저토심 옥상녹화 시스템 을 연구하고 개발하고자 수행되었다. 연구목적을 달성하기위해서 시스템의 개념적 모델이 선행 연구로부터 유추되었고 개념적 모델로부터 실험을 위한 시스템들이 제안되었다. 건축물 옥상 위에 설치된 이 시스템들에서 기린초의 생육에 대하여 인공배지 종류, 토심, 그리고 배수 형태의 효과들이 2002년 4월 3일부터 10월 18일까지 연구되었다. 인공배지 종류는 단용과 혼용이고, 토심은 5cm, l0cm,그리고 15cm이며, 배수 형태는 저수$.$배수형과 배수형으로 하였다. 여기서, 인공배지 단용은 폐유리 미분 100에 발포제를 1∼2정도 첨가하고, 착색제를 1정도 첨가한 후, 6∼8$^{\circ}C$/min로 승온하여 750∼85$0^{\circ}C$의 온도에서 발포시킨 다공질 유리를 수냉식으로 급랭하고, 분쇄기로 이송하여 l0mm이하로 분쇄하고 입도를 조절하여 얻어진 다공질 유리 파쇄물과 수피를 부피 비 6:4로 혼합하여 조성된 것이며 인공배지 혼용은 인공배 지 단용에 양토(모래 46%, 미사 40%, 점토 14%)를 부피비 5:5로 혼합하여 조성된 것이다. 피복면적, 지상부와 지하부의 생체중과 건물중, 그리고 시각적 질을 조사하였다. 각 변수들은 던칸의 다중범위 검정으로 통계처리 하였으며 처리들간의 유의수준은 5%였다. 그리고 기존 건축물 옥상에 대한 과부하의 위험을 피하기 위해서 각 시스템의 중량이 평가되었다. 그 결과를 요약하면 다음과 같다. 실험기간 중에 피복면적에 대한 배수 형태의 효과는 유의성 있는 차이를 나타내지 않았다. 인공배지 혼용의 피복면적은 인공배지 단용의 것보다 통계적으로 유의성 있게 높았다. 토심 5cm처리의 피복면적은 나머지 처리들의 피복면적보다 통계적으로 유의성 있게 낮았다. 토심 l0cm처리와 토심 15cm처리는 통계적으로 유의성이 없는 것으로 나타났다. 지상부와 뿌리의 생체중 및 건물중과 시각적 질에 대한 처리들의 효과는 피복면적에 대한 것과 유사하거나 동일하였다. 결과적으로, 기린초의 생육은 인공배지 단용보다 인공배지 혼용에서 더 높았고, 토심 10∼15cm에서 더 높았으며, 배수 형태에서는 뚜렷한 차이를 나타내지 않았다. 이 결과를 토대로, 기존 건축물 옥상에 적용 가능한 시스템의 허용하중과 기린초의 생육을 동시에 고려해볼 때, 저토심 옥상녹화 시스템 은 인공배지 종류에서는 혼용이, 토심은 10cm, 그리고 배수형태는 배수형이 적합하다고 보았다. 제안된 조건으로 조성된 시스템은 인공배지가 포장용수량상태일 때 그 중량이 약 115kg/$m^2$정도로 나타났다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.5
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• pp.405-411
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• 1994

This study was carried out to determined the optimum seeding rate and number of seedling stands per unit area for high yield with two varieties of rice, Nakdongbyeo (Japonica type) and Samgangbyeo (Indica x Japonica type), in direct water-seeded rice culture at Crop Experiment Station. Seeds coated with $CaO_2$ after germination were sowed at May 4 in 7 levels, such as 1.6, 1.8, 2.1, 2.7, 3.5, 4.0, 4.3kg /10a, and the number of seedling stands per square meter was adjusted to be 30, 40, 60, 80, 100, 120, 140 plants, respectively. The maximum number of tillers increased as the seeding rates increased, but the ratio of effective tillers decreased. The critical dates of effective tillering of tested varieties were delayed by the lower seeding rates, and the date was June 18 at the plot of seeding rate with 3.5kg /10a. The number of panicles per unit area increased in accordance with the increased seeding rates. The number of spikelets per panicle in Nakdongbyeo was not affected by the seeding rates, but in Samgangbyeo it decreased as the seeding rate increased. The optimum number of seedling stand per square meter appeared to be 78 in Nakdongbyeo and 120 in Samgangbyeo, respectively. It meant that the optimum seeding rates for Nakdongbyeo and Samgangbyeo were about 2.7 and 4.0kg /10a, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.4
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• pp.284-291
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• 2010

A simulation modeling for predicting the harvesting date with high potato solids consists of development of mathematical models. The mathematical model on potato growth and its development should be obtained by using agricultural elements which analyze relations of solar radiation quantity, temperature, photon quantity, carbon dioxide exchange rate, water stress and loss, relative humidity, light intensity, and wind etc. But more reliable way to predict harvesting date against climatic change employs in vivo energy consumption for growth and induction shape in a slight environmental adaptation. Therefore, to calculate in vivo energy loss, we take a concept of estimate of the amount of basal metabolism in each tuber on the basis of $Wm={\int}^m_tf(x)dt$ and $Tp=\frac{Tm{\cdot}Wm^{Tp}}{Wm^{Tm}}$. In the validation experiments, results of measuring solid accumulation of potato harvested at simulated date agreed fairly well with the actual measured values in each regional field during the growth period of 2005-2009. The calculation method could be used to predict an appropriate harvesting date for a production of high potato solids according to weather conditions.

• Reproductive and Developmental Biology
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• v.32 no.3
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• pp.159-166
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• 2008

We report here the generation of transgenic chickens that produce human Thrombopoietin (hTPO) using replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G). For the retrovirus vectors, we used hCMV (human Cytomegalovirus) internal promoter to drive the hTPO gene. After confirming the expression of the hTPO gene in various target cells, the concentrated solution of recombinant retrovirus was injected beneath the blastoderm of non-incubated chicken embryos (stage X). The biological activity of the recombinant hTPO in target cell was significantly higher than its commercially available counterpart. Out of 132 injected eggs, 11 chicks hatched after 21 days of incubation and 4 hatched chicks were found to express vector-encoded hTPO gene. However, 3 out of the 4 transgenics died within one month of hatching. The major significance of this study is that it is one of the very few successful reports on the production of transgenic chickens as bioreactors aiming mass production of commercially valuable and biological active human cytokine proteins.

• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2009.12a
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• pp.292-292
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• 2009

본 연구는 유기종자 안전 생산기술 개발을 위한 효과적인 병해충 제어를 위해 대표적인 찰옥수수 품종인 미백2호와 흑미찰을 대상으로 병?해충 발생양상을 조사하였다. 2008년 유기농 찰옥수수 종자 생산지의 우점 비산포자의 종류 중Alternaria sp., Cladosporium sp., 및 Helminthosoprium sp.,은전 생육기간에 관찰되었다. 또한종자로부터 신초 출현 전후로 종자가 발아하지 않고 부패하거나 유묘의 시들음 증상, 심하면 고사하는 증상과 거세미나방에 의해 지재부가 절단된 전형적인 피해를 관찰할 수 있었고 발병주율은 14~6% 미만이었다. 특히, 포장내 조명나방 발생밀도는 2008년 트랩당 3.5~0.5 마리로 높게 나타났으며, 2009년 트랩당 0.5~6마리로 낮게 나타났다. 또한 피해주율은 2008년은 7.5(미백2호)~21(미흑찰)%였으며, 2009년은 1~46%로 매년 높은 피해주율을 보였으며 수확기까지 지속적으로 증가하였다. 옥수수잎마름병 (Leaf blight)의 발생 양상은 2008년도에 7%(미흑찰)~34%(미백2호)로 높게 나타났으며, 2009년은 6월18일 이후 초발생하여 발병주율이 8월 21일 (59%, 미흑찰)이후 급격히 증가하여 9월 25일이는 미흑찰 (69%)의 발병주율이 미백2호 (56%)보다 높게 나타났다. 옥수수깜부기병(Common Smut)의 발생 양상은 2008년도에 20주 중 2%~15%의 발병주율을 보였으며, 2009년은 초발생 시기가 7월17일로 2008년에 비해 17일 정도 늦추어졌으며 발병주율도 8% 미만 (9월 25일)으로 2008년에 비해 아주 낮게 나타났다. 옥수수깨씨무늬병 (Southern Leaf Spot)의 발생 양상은 2008년 20주 중 초기에 평균 11%(미흑찰)에서 증가하여 9월 19일 조사시에는 두 품종 모두 62%로 높게 나타났다. 2009년은 6월8일 이후 초발생하였으며 9월 25일까지 지속적 발병하여 약 86%의 발병 주율을 보였고 2008년에 비해 24% 이상 발병주율이 높았다.

• Korean Journal of Weed Science
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• v.4 no.2
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• pp.194-203
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• 1984

To evaluate the compatibility in systemic use of 4 kinds of herbicides on both weeds and soybeans, trials were conducted at Coll. Exp. Farm/Jonnam Nat'l. Univ., 1983. Among the experimented 4 herbicides, alachlor or metribuzin were applied at pre-emergence, and followed by postemergence spraying of acifluorfen or bentazon, respectively. All herbicides were applicated at rates of 0, 0.25, 0.5, and 0.75 kg active ingredients per ha. Under the conditions which Echinochloa crusgalli and Digitaria adscendense were dominating, most comprehensive compatibility was found from each rates 0.5 - 0.75 kg/ha at 60 days after crop seeding date. However, slight transient leaf burn was evident at the plots of metribuzin and/or acifluorfen applicated. The positive tendency of herbicide interactions in weeding efficacy was observed from the system in alachlor sequence. However, the results in crop growth and yields at hervest indicate the necessity of higher rates of each herbicides upto 0.75 kg/ha for the consistence weeding efficacies. And more excellent weeding results (crop growth and yields) on crop plants were provided from the plots which applicated by residual type herbicide, namely acifluorfen than bentzaon.

• Journal of Food Hygiene and Safety
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• v.23 no.4
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• pp.343-347
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• 2008

Recently, Sunshik has been issued because of easy-cook and well-being food. Sunshik basically was made of the heated cereals. Amount of spore-forming Bacillus cereus was detected and it has been caused some problem of food safety. B. cereus was isolate from 57 out of 161 Sunshik samples resulting in the isolation rate of 35.4%. Quantitative analysis of 57 samples showed that 21 samples were less than 100 CFU/g, 33 samples were between 100 and 1,000 CFU/g and distinctively even 3 (1.9%) samples had over 1,000 CFU/g. Typical morphology of B. cereus isolated from Sunshik was observed on MYP agar and then further characteristics was identified by using VITEK 2 (Biomeriux, France). 53 strains out of 57 strains isolated from Sunshik (about 93.0%) produced diarrheal enterotoxin in brain heart infusion broth which was detected by the Bacillus cereus enterotoxin reversed passive latex agglutination test kit (Oxoid England). The D-values of the B. cereus spores were $75^{\circ}C$ (37.1mim), $80^{\circ}C$ (22.5mim), $85^{\circ}C$ (4.9mim), and $90^{\circ}C$ (3.1mim) respectively. The Z-value was calculated $12.8^{\circ}C$ in Sunshik sample inoculated with B. cereus. Therefore, the management of B. cereus in Sunshik is required for the food-safety.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.3
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• pp.348-353
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• 1996

Mutation tachniques inducing more useful mutations and reducing somatic effects need to be improved for crop breeding. Seeds of barley varieties ; Dema, Grosso were treated with two types of mutagens ; 1) chemical treatment: single treatment or double treatment of two mutagens (N-nitroso-N-methylurea ; MNH, Sodium Azide; NaN$_3$) 2) gamma ray irradiation treatment. After treatment, half of seeds were used for germination test and half of seeds were sown to the field. With the higher dose of mutagen both chemical and gamma ray were plants treated, the higher rate of growth reduction rate was in M$_1$ seedling. In chemical treatment, germination rate of seeds, growth rate of coleoptile and root in double treatment of chemical mutagens were better than single treatments, especially in same dose. Growth inhibition rate of plant in double treatment of 1.0mM MNH(0.5mM MNH + 0.5mM MNH), for example, were less than one of plants of single treatment of 1.0mM MNH in pot and petri dish test. Growth reduction rate of culm and fertility rate in M$_1$ plants double treated in same dose of single treatment were also less than single one. With the higher dose of mutagen both chemical and gamma ray were plants treated, the higher frequency of chlorophyll mutants was in M$_2$ seedling. The rate of chlorophyll mutants in double treatment of chemical mutagens were higher than single treatment. Double treatment methods can be a improved method for induction of new good mutants, which were induced more useful mutations and reduced harmful somatic effects.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.1
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• pp.28-33
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• 1996

This experiment was carried out at National Crop Experiment Station in 1994 to obtain basic information of growth characters and yield of rice under various seeding methods in direct seeding on dry paddy. Hwaseongbyeo sown on April 27 by flat drill seeding, high ridged drill seeding and hill seeding under different seed conditions, soil convering after seeding and irrigation methods. Nitrogen applied 150kg /ha by 3 times split application at rate of 40:30:30％ (basal: 5th leaf stage:panicle initiation stage). Seedling stands were higher in flushed irrigation water after seeding than in irrigation at 3rd leaf stage, and it also was higher in high ridged drill seedings than in flat drill seedings. Days for seedling stand, heading date were shorten 6~10 days, 2~3 days by flushed irrigation water after seeding, respectively. Number of tiller was more in high ridged drill seedings than conventional seedling method(Flat drill seeding ＋ intact seed ＋ soil covering after seeding ＋ irrigation at 3rd leaf stage after seedling stand) at early growth stage, but the most tiller number was highest in developed seeding method(Flat drill seeding ＋ soaked seed ＋ non soil covering after seeding ＋ fulshed irrigation water after seeding) at heading stage among used seeding methods. Lodging index was slightly high in high ridged drill seeding and conventional seeding. Field lodging was degree 1 in high ridged drill seedings and developed seeding, Milled rice yield in developed seeding was increased 7％ compaired with conventional seeding(5.35t /ha).