• Landscaping Tree
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• s.99
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• pp.47-48
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• 2007

• Korean Journal Plant Pathology
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• v.12 no.2
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• pp.197-201
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• 1996

우리나라 포도재배 지역인 포천, 남양주 및 수원의 포도나무 줄기혹병에 이병된 포장으로부터 채집한 줄기혹(gall), 수액(sap) 및 근권토양으로부터 56균주의 유사 Agrobacterium를 분리하였으며, 이들 중 7균주가 포도나무 유묘의 줄기에 혹ㅇ르 형성시키는 병원성 Agrobacterium biovar의 선택배지에서의 생장, oxidase의 생성, Na L-tartrate로부터 알칼리의 성장, 2% NaCl 첨가 배지에서 생장, 3-ketolactose의 비생산, mesoerythritol과 ethanol로부터 산을 생성하지 못하는 등 Agrobacterium biovar 3인 A. vitis로 동정되었다.

• Research in Plant Disease
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• v.12 no.1
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• pp.10-14
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• 2006

This study was carried out to know damages of the grapevine trees by the disease to the leaf spot from 2000 to 2003. The isolates collected from different varieties and locations were identified as Pseudocercospora vitis ($(L\acute{e}v)$.) Speg. based on the morphological and cultural characteristics. According to the in vitro test, the range of temperatures for the mycerial growth and the conidical germination of the fungus were from $10^{\circ}C\;to\;35^{\circ}C$ and optimum temperature was $25^{\circ}C$. There were remarkably different features between a low infection trees group and high infection trees group in terms of number of leaves per fruit branch, length and diameter of internode and leaf area.

• Research in Plant Disease
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• v.14 no.2
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• pp.90-94
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• 2008

To investigate the severity of crown gall disease on grapevine, the ratio of healthy vs. galled grapevines and the presence of the pathogen of the disease in soil were measured in Korean vineyards. In field and greenhouse cultivations, the crown gall incidence of the cv. Kyoho grapevines was $0.4{\sim}97.9%$ and $1.4{\sim}3.8%$ and those of cv. Campbell Early was $1.2{\sim}2.1%$ and $0{\sim}1.8%$, respectively. The higher populations of Agrobacterium spp. were isolated from soils of grapeveins with crown gall than from soils of noninfected vineyards. Based on the colony shapes and growth on plates, 480 isolates of Agrobacterium spp. from 21 soil samples were collected. Only 13 isolates out of 480 developed the gall on inoculated grapevines.

• Korean journal of applied entomology
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• v.41 no.2
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• pp.137-143
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• 2002

Grape phylloxera, Daktulosphaira vitifoliae(Fitch), is a destructive grape insect pest because it forms root galls and kills the plant by directly destroying root system. The effects of insecticide treatments were examined to establish the chemical control system of phylloxera, and the damage by this insect was investigated in grapevine yards. The treatments of 40kg carbofuran 3GR per ha and carbosulfan 20WP of 10$\ell$ (1,000$\times$) per tree successfully reduced phylloxera densities. After the insecticide treatments in early season, the phylloxera population rebuilt up again at harvest season. In a grapevine yard where two times of carbofuran treatment (mid-May and post-harvest treatment) were applied a year from 1998 to 2000, total yield per ha in 2000 showed an increase of 30% over that of 1998. But the yield in 2000 was low 20% compared to the average standard yield of Korean grapevine yards. In a grapevine yard without chemical treatment during the same years, total yield per ha in 2000 showed 53% reduction compared to that of 1998. Also, damaged vines by phylloxera abruptly increased from 3% in 1998 to 50% in 2000.

• Research in Plant Disease
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• v.10 no.4
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• pp.341-344
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• 2004

Grapevine leaf spot caused by Pseudocercospora vitis also called leaf Isariopsis spot which is one of the most important disease in Korea. Spots on the leaves are irregular to angular. They are brown, measure 2${\sim}$20 mm in diameter. Campbell Early, the major grape cultivar in Korea, is very susceptible to this disease. Leaf spot of grape is outbreak about 95% in ratio of diseased leaves according to the areas and management state of orchards in Korea. The diseased grapevines have some difficulty in raise of the trees by early defoliation and decrease the quality of fruits by disadvantage of assimilation products accumulation. Severe outbreak of the grapevine leaf spot is one of the factors that decrease of sugar content of the fruits about 20%. The strong negative correlation was observed between disease increase of grapevine and decrease of sugar content of the fruits. The correlation equation between relative sugar content of fruits and accumulated percentage of diseased leaves was Y = 0.0009$X^2$ - 0.3717X + 116.45($r^2$= 0.98).

• Korean journal of applied entomology
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• v.48 no.4
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• pp.467-477
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• 2009

Host preference was tested on the 7 species plants against ggot-mae-mi, Lycorma delicatula (Hemiptera: Fulgoridae). This insect highly preferred Ailanthus altissima and Vitis vinifera however, didn't choose the other plants preferentially. Both nymphs and adults lived longest in A. altissima and V. vinifera but lived in short and low ecdysis rate against other plants and 3 species fruits. By analyzing the phloem-feeding behavior using EPG, L. delicatula was showed the short time in non-probing phase and it also exhibit the longest feeding time in A. altissima and V. vinifera, but other plants did not feed the phloem at all. In sugar contents analysis, A. altissima existed high sucrose proportion and followed by fructose>glucose, V. vinifera was analyzed by an order of glucose> fructose>maltose>sucrose>rhamnose, Malus pumila was as glucose> fructose, Pyrus calleryana was as glucose>unkown>fructose, Hibiscus syriacus was as sucrose>glucose. Nymphs and adults of L. delicatula lived longest in 5% sucrose solution, and next is in 5% fructose solution. However, they lived short in other sugar solutions. L. delicatula nymph and adult according to the combination of sugar proportion found in original plants lived longer in sugar combination solution of A. altissima and those of V. vinifera was next. Analyzed original sugar proportion from M. pumila, P. calleryana, H. syriacus respectively, L. delicatula lived short period comparing to the A. altissima, V. vinifera. This result was judged that sugar contents affected on choosing the host plants.

• Research in Plant Disease
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• v.15 no.2
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• pp.77-82
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• 2009

The roots of grapevine in the field in which the crown gall was occurred severely in Chungbuk province were collected and Agrobacterium spp. were isolated from the roots using the selective media. The selected 13 isolates were identified as A. tumefaciens with fatty acid analysis using MIDI system, nucleotide sequence of 16S rDNA, biochemical characteristics, and PCR with the species-specific primers. A. vitis, a pathogen of crown gall disease of grapevine was not isolated from the roots. All of the isolates did not show pathogenicity on the tomato seedlings and the stem and root of grapevine. Eric-PCR showed that DNA band patterns of the root isolates were a little more similar to A. tumefaciens than A. vitis. However, overall similarity between the root isolates and the pathogenic strains of A. tumefaciens and A. vitis was low by rep-PCR. These results suggest that a pathogen causing crown gall in grapevine in Chungbuk province may transmitted through the seedlings rather than via soil or roots.

• The Korean Journal of Pesticide Science
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• v.11 no.2
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• pp.82-86
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• 2007

Methanol extract obtained from aerial parts of Vitis vinifero L. was successively fractionated with n-hexane, ethylacetate, n-butanol, and water. From ethylacetate fraction, an active compound was isolated through silica gel column chromatography and recrystallization, and was identified as Lup-20(29)-ene-3,28-diol on the basis of EI-MS data. The compound, at 100 mg $mL^{-1}$, inhibited the mycelial growth of Phytophthora capsici and Colletotrichum acutatum by 52.1 % and 40.8%, respectively.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.13 no.5 s.110
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• pp.42-49
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• 1992