The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Characterization of an Antifungal Substance Isolated from Aerial Parts of Vitis vinifero L.

포도나무 (Vitis vinifero L.) 지상부로부터 분리한 항진균성 활성물질의 특성규명

  • Lim, Tae-Heon (Research Institute of Agri-Bio Science, SamHoUB Co.) ;
  • Youl, Kwon-Soon (Regional Innovation Center, Sangju National University) ;
  • Choi, Yong-Hwa (Dept. of Plant Resources, Sangju National University)
  • 임태헌 ((주) 삼호유비 농생명과학연구소) ;
  • 권순열 (상주대학교 지역혁신센터) ;
  • 최용화 (상주대학교 식물자원학과)
  • Published : 2007.06.30
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Abstract

Methanol extract obtained from aerial parts of Vitis vinifero L. was successively fractionated with n-hexane, ethylacetate, n-butanol, and water. From ethylacetate fraction, an active compound was isolated through silica gel column chromatography and recrystallization, and was identified as Lup-20(29)-ene-3,28-diol on the basis of EI-MS data. The compound, at 100 mg $mL^{-1}$, inhibited the mycelial growth of Phytophthora capsici and Colletotrichum acutatum by 52.1 % and 40.8%, respectively.

포도나무 잎의 메탄올 추출물을 n-hexane, ethylacetate, n-butanol $H_2O$으로 순차적으로 용매분획 하였다. Ethylacetate 분획으로부터 silica gel chromatography와 재결정하여 활성물질을 분리 정제하였다. 이 화합물의 화학구조를 EI-MS의 조각 데이터를 근거로 분석한 결과 betulin으로 구조결정 되었다. 이 화합물이 고추 역병균(Phytophthora capsici), 고추 탄저병균(Colletotrichum acutatum)에 대한 항균활성(in vitro)을 측정한 결과, 100 ${\mu}g\;mL^{-1}$에서 각각 52.1%, 40.8%의 균사생육 억제효과를 보였으나, 잔디 마름병(Rhizoctonia solani) 균의 균사생육 억제 효과는 나타나지 않았다.

Keywords

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